SRR3554393_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554393_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	316166
Sequences flagged as poor quality	0
Sequence length	51
%GC	41

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11339	3.5864071405527476	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCG	1816	0.5743818120860561	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGC	1761	0.5569858871605423	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTC	1673	0.5291524072797201	No Hit
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	1361	0.4304700695204418	Illumina PCR Primer Index 5 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCATGATTT	673	0.212862863179469	No Hit
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	438	0.13853482031591	Illumina PCR Primer Index 5 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCATGATTTCGTA	410	0.129678713081103	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCC	409	0.12936242353700272	No Hit
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT	402	0.12714839672830094	Illumina PCR Primer Index 5 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCT	387	0.12240405356679718	No Hit
GAACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT	340	0.10753844499408538	Illumina PCR Primer Index 5 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	326	0.10311039137668188	Illumina PCR Primer Index 5 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGAG	20	7.02552E-4	45.000004	16
CGAATGC	35	1.208191E-7	45.000004	45
GGGCCTA	20	7.02552E-4	45.000004	7
GCTAGCG	20	7.02552E-4	45.000004	1
GCGCTTT	20	7.02552E-4	45.000004	21
TAGTACG	20	7.02552E-4	45.000004	1
CACGGGT	25	3.88437E-5	45.0	4
ATTCGGT	25	3.88437E-5	45.0	13
CGTTATT	95	0.0	45.0	1
TGCGATG	25	3.88437E-5	45.0	1
CTACGGG	45	3.8380676E-10	45.0	3
TGCGAAG	25	3.88437E-5	45.0	1
CGGACGG	45	3.8380676E-10	45.0	2
CGTTTTT	5540	0.0	43.294228	1
ACGGGCC	55	6.002665E-11	40.909092	5
CCCGCAT	55	6.002665E-11	40.909092	40
TCGGCGT	50	1.0768417E-9	40.5	4
CGTAAGG	50	1.0768417E-9	40.5	2
TTACGGG	50	1.0768417E-9	40.5	3
GATGAAT	435	0.0	39.827587	20