SRR3554391_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554391_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	258765
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5001	1.9326415859950148	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGC	3060	1.182540142600429	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCG	2672	1.0325971441269104	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTC	2514	0.9715378818619211	RNA PCR Primer, Index 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCTGC	2342	0.905068305219021	RNA PCR Primer, Index 40 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCTGC	1206	0.46605993855428673	RNA PCR Primer, Index 1 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCTGCT	1159	0.4478967402855873	TruSeq Adapter, Index 1 (96% over 27bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCAGGATCT	993	0.38374586980464903	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCAGGATCTCGTA	808	0.31225242981083223	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCT	733	0.2832686027863119	TruSeq Adapter, Index 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCTGC	574	0.22182288949432882	RNA PCR Primer, Index 40 (96% over 27bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTC	573	0.2214364384673352	RNA PCR Primer, Index 40 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCT	504	0.19477131760477653	RNA PCR Primer, Index 40 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCC	465	0.17969972755202596	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	374	0.144532684095608	No Hit
ACTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCTGC	370	0.14298687998763357	RNA PCR Primer, Index 40 (96% over 27bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	295	0.11400305296311325	No Hit
CGCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCTG	277	0.10704693447722838	TruSeq Adapter, Index 1 (96% over 25bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCCAT	20	7.0231454E-4	45.0	37
TCGTTGC	20	7.0231454E-4	45.0	42
CTGTATC	20	7.0231454E-4	45.0	22
CCCTCGA	20	7.0231454E-4	45.0	41
ATTAGGC	20	7.0231454E-4	45.0	23
CTCGTCT	20	7.0231454E-4	45.0	22
ACTTGCG	20	7.0231454E-4	45.0	14
TCCGACT	20	7.0231454E-4	45.0	11
ATCAGGT	20	7.0231454E-4	45.0	25
CGTTGCC	20	7.0231454E-4	45.0	43
CCAATGG	20	7.0231454E-4	45.0	2
TAAGTTA	20	7.0231454E-4	45.0	44
TAACGTT	20	7.0231454E-4	45.0	17
AGTATAC	20	7.0231454E-4	45.0	20
GTGGACC	20	7.0231454E-4	45.0	14
TTGAGCG	20	7.0231454E-4	45.0	1
CTTGCGT	20	7.0231454E-4	45.0	15
TCTCGTC	20	7.0231454E-4	45.0	21
TCGCTCG	20	7.0231454E-4	45.0	22
GTTCCGA	20	7.0231454E-4	45.0	9

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content