##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554391_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	258765
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.899217436670337	33.0	31.0	34.0	30.0	34.0
2	32.00448283191312	33.0	31.0	34.0	30.0	34.0
3	32.01460784882036	33.0	31.0	34.0	30.0	34.0
4	35.67667961277607	37.0	35.0	37.0	33.0	37.0
5	35.755484706200605	37.0	35.0	37.0	35.0	37.0
6	35.77483044461191	37.0	35.0	37.0	35.0	37.0
7	35.93001758352173	37.0	35.0	37.0	35.0	37.0
8	35.80889223813112	37.0	35.0	37.0	35.0	37.0
9	37.634958359901844	39.0	37.0	39.0	35.0	39.0
10	37.42285084922613	39.0	37.0	39.0	34.0	39.0
11	37.37601298475451	39.0	37.0	39.0	34.0	39.0
12	37.28004946573146	39.0	37.0	39.0	34.0	39.0
13	37.1771066411609	39.0	37.0	39.0	33.0	39.0
14	38.33351110080575	40.0	38.0	41.0	33.0	41.0
15	38.37432032925628	40.0	38.0	41.0	34.0	41.0
16	38.50333700461809	40.0	38.0	41.0	34.0	41.0
17	38.41784244391629	40.0	38.0	41.0	34.0	41.0
18	38.30452727378123	40.0	38.0	41.0	34.0	41.0
19	38.11418082043553	40.0	37.0	41.0	34.0	41.0
20	37.78245125886422	40.0	35.0	41.0	34.0	41.0
21	37.80847487102197	40.0	35.0	41.0	34.0	41.0
22	37.852120650010626	40.0	35.0	41.0	34.0	41.0
23	37.721724344482446	40.0	35.0	41.0	34.0	41.0
24	37.65427318223098	39.0	35.0	41.0	33.0	41.0
25	37.61555465383649	39.0	35.0	41.0	33.0	41.0
26	37.62292041041099	39.0	35.0	41.0	33.0	41.0
27	37.51333449268641	39.0	35.0	41.0	33.0	41.0
28	37.40001159353081	39.0	35.0	41.0	33.0	41.0
29	37.384464668714855	39.0	35.0	41.0	33.0	41.0
30	37.15312735493595	39.0	35.0	41.0	33.0	41.0
31	36.93136629760593	39.0	35.0	41.0	32.0	41.0
32	36.594860201340985	39.0	35.0	41.0	31.0	41.0
33	36.286093559793635	39.0	35.0	41.0	30.0	41.0
34	35.88760844781945	39.0	35.0	41.0	27.0	41.0
35	35.74077638011323	39.0	35.0	41.0	25.0	41.0
36	35.59320619094545	39.0	35.0	41.0	24.0	41.0
37	35.495016714006915	39.0	35.0	41.0	24.0	41.0
38	35.39227097946013	39.0	35.0	41.0	23.0	41.0
39	35.271249975846814	39.0	35.0	41.0	23.0	41.0
40	35.19679632098622	39.0	35.0	41.0	23.0	41.0
41	35.096720963035956	39.0	35.0	41.0	23.0	41.0
42	35.11415376886364	39.0	35.0	41.0	23.0	41.0
43	35.05980716093753	39.0	35.0	41.0	22.0	41.0
44	35.00250033814465	39.0	35.0	41.0	22.0	41.0
45	34.93277684385446	38.0	35.0	41.0	22.0	41.0
46	34.8530365389446	38.0	35.0	41.0	22.0	41.0
47	34.71322242188859	38.0	35.0	40.0	22.0	41.0
48	34.737850945838886	38.0	35.0	40.0	22.0	41.0
49	34.71751975730876	38.0	35.0	40.0	21.0	41.0
50	34.62597337352424	38.0	35.0	40.0	21.0	41.0
51	33.84766873417966	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	1.0
11	5.0
12	8.0
13	6.0
14	3.0
15	11.0
16	16.0
17	52.0
18	85.0
19	163.0
20	309.0
21	550.0
22	1032.0
23	1562.0
24	2267.0
25	3430.0
26	4833.0
27	5499.0
28	5070.0
29	4489.0
30	4431.0
31	5024.0
32	6259.0
33	8092.0
34	14585.0
35	21093.0
36	18493.0
37	23790.0
38	43169.0
39	84334.0
40	98.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.958669062663034	17.622166830908352	20.95762564489015	18.461538461538463
2	37.27745251483006	27.158232373002534	20.108979189612196	15.455335922555214
3	27.32556566769076	27.108380190520354	31.144088265414567	14.421965876374315
4	24.91101965103472	21.831777867949683	36.714006917473384	16.54319556354221
5	26.679419550557455	25.392924081695746	30.021061580971153	17.906594786775646
6	22.307112631151817	33.902575696094914	29.87962050509149	13.91069116766178
7	66.54802620137963	5.549436747628157	22.946302629799238	4.956234421192975
8	64.04034548721813	9.660889223813113	19.002569899329508	7.296195389639248
9	58.46965393310533	7.228566459915367	21.615751743860258	12.686027863119046
10	34.87140842076788	22.388653797847468	27.809402353486757	14.930535427897901
11	27.63627229339362	22.136687728247637	31.87564005951346	18.351399918845285
12	23.773694278592544	18.57360925936661	36.520781403976585	21.131915058064266
13	23.566170077096977	21.762216683090834	38.67833748768187	15.993275752130312
14	17.19513844608042	28.257685544799337	33.98373041176357	20.563445597356676
15	14.471818058856492	22.56564836821054	43.37371746565417	19.588816107278806
16	16.40175448766255	23.086970803624908	37.151855931057135	23.359418777655403
17	16.704345641798543	23.72693370432632	35.5349448341159	24.03377581975924
18	18.187544683399995	21.579811798349855	37.94794504666396	22.28469847158619
19	18.839487565938207	23.992039108843933	33.87629702625935	23.292176298958513
20	21.448031998145034	23.12020559194636	36.597685158348305	18.834077251560295
21	19.338395841786948	27.48748864800108	34.51278186771781	18.661333642494153
22	16.81873514578865	22.60236121577493	35.009371437404596	25.569532201031826
23	17.16731397213688	26.10128881417502	34.36361177129828	22.367785442389813
24	18.859583019341876	22.578014801074335	34.69866481170173	23.863737367882052
25	16.67613471682801	26.53488686646185	33.41526095105598	23.373717465654163
26	16.01568991169594	23.297586613336424	37.37367882055146	23.313044654416167
27	20.015458041079746	24.695766428999285	33.12464977875679	22.164125751164185
28	14.941742507680713	26.546480397271655	36.19693544335594	22.31484165169169
29	19.478291113558637	21.55662473673024	36.06167758390818	22.903406565802946
30	20.350897532510192	25.05477943307634	32.79809866094719	21.796224373466274
31	19.945510405193904	26.55961973218944	29.674801460784884	23.82006840183178
32	22.63327729793442	25.018453036538947	31.420787200741984	20.92748246478465
33	21.881243599404865	23.81079357718393	28.133248314107394	26.174714509303808
34	19.74146426294128	25.71367843410044	32.15311189689486	22.391745406063414
35	20.850965161439916	21.843371398759494	31.479141305818022	25.82652213398257
36	20.342395609916334	26.90085598902479	29.831700577744286	22.92504782331459
37	20.66778737464495	25.859756922304022	30.896373157111668	22.576082545939364
38	21.65864780785655	27.02954418101366	27.77694046721929	23.534867543910497
39	24.162850462775104	24.19763105520453	28.870983324638182	22.768535157382182
40	23.256622804475104	22.3202519660696	32.87384306223794	21.54928216721736
41	17.927849593260294	25.064440708751185	29.299171062547096	27.70853863544142
42	19.901068537089635	21.967035727397448	33.72596757675883	24.40592815875408
43	22.2901087859641	21.759511525901882	29.37839352308079	26.571986165053236
44	21.735551562228277	23.579695863041756	29.77759743396518	24.907155140764786
45	21.305431569184393	21.20379494908508	30.481324754120536	27.009448727609993
46	24.72011284369988	23.461828299808708	29.624176376248716	22.193882480242692
47	18.3927501787336	23.30570208490329	35.357563812725836	22.943983923637276
48	19.747261028346184	22.62902633663749	31.336154425830387	26.287558209185942
49	21.010569435588273	18.88277008096149	35.3050064730547	24.80165401039553
50	20.61329777983885	19.45935501323595	33.423376422622844	26.50397078430236
51	19.451625992696076	19.364674511622514	29.969663594381004	31.214035901300406
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	207.0
1	286.5
2	366.0
3	4119.5
4	7873.0
5	5048.5
6	2224.0
7	2230.5
8	2237.0
9	2275.0
10	2313.0
11	2241.0
12	2169.0
13	2079.5
14	1990.0
15	1847.5
16	1705.0
17	1656.0
18	1607.0
19	1500.5
20	1394.0
21	1314.5
22	1235.0
23	1297.0
24	1359.0
25	1448.0
26	1713.0
27	1889.0
28	2054.5
29	2220.0
30	2493.0
31	2766.0
32	3065.5
33	3365.0
34	3697.0
35	4029.0
36	4448.5
37	4868.0
38	5407.0
39	5946.0
40	6878.5
41	7811.0
42	9719.5
43	11628.0
44	13480.5
45	15333.0
46	24909.5
47	34486.0
48	33142.0
49	31798.0
50	30881.5
51	29965.0
52	24796.5
53	19628.0
54	16511.5
55	13395.0
56	12139.0
57	10883.0
58	9488.5
59	8094.0
60	7367.0
61	6640.0
62	5877.5
63	5115.0
64	4612.0
65	4109.0
66	3157.5
67	2206.0
68	1831.0
69	1456.0
70	1292.0
71	1128.0
72	939.5
73	751.0
74	625.0
75	381.0
76	263.0
77	206.5
78	150.0
79	127.0
80	104.0
81	60.5
82	17.0
83	9.0
84	1.0
85	3.5
86	6.0
87	3.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	258765.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.153020080510906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.74477765140848	29.26483780298919
2	10.863967682184784	8.507142896292049
3	4.080279029654393	4.792657403464381
4	2.076999967920808	3.252832858048957
5	1.2909963180048596	2.5273202381354958
6	0.8759973985655706	2.057876624190786
7	0.6529980576181125	1.7896792243719601
8	0.5409983906584468	1.6945376682379405
9	0.4599986316096108	1.6209302094376772
>10	4.283987256119264	31.202123473591804
>50	0.08099975904427167	2.1029024528711613
>100	0.03499989588332727	2.5190978182411192
>500	0.005999982151427532	1.6385490160614056
>1k	0.005999982151427532	5.0714756045503915
>5k	9.99997025237922E-4	1.9580367095156728
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5001	1.9326415859950148	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGC	3060	1.182540142600429	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCG	2672	1.0325971441269104	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTC	2514	0.9715378818619211	RNA PCR Primer, Index 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCTGC	2342	0.905068305219021	RNA PCR Primer, Index 40 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCTGC	1206	0.46605993855428673	RNA PCR Primer, Index 1 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCTGCT	1159	0.4478967402855873	TruSeq Adapter, Index 1 (96% over 27bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCAGGATCT	993	0.38374586980464903	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCAGGATCTCGTA	808	0.31225242981083223	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCT	733	0.2832686027863119	TruSeq Adapter, Index 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCTGC	574	0.22182288949432882	RNA PCR Primer, Index 40 (96% over 27bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTC	573	0.2214364384673352	RNA PCR Primer, Index 40 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCT	504	0.19477131760477653	RNA PCR Primer, Index 40 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCC	465	0.17969972755202596	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	374	0.144532684095608	No Hit
ACTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCTGC	370	0.14298687998763357	RNA PCR Primer, Index 40 (96% over 27bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	295	0.11400305296311325	No Hit
CGCTGTCTCTTATACACATCTGACGCTCAGGATCTCGTATGCCGTCTTCTG	277	0.10704693447722838	TruSeq Adapter, Index 1 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4556257608254594	0.0
2	0.0	0.0	0.0	2.2441211137518597	0.0
3	0.0	0.0	0.0	2.964465828067938	0.0
4	0.0	0.0	0.0	3.949143044847642	0.0
5	0.0	0.0	0.0	6.7895580932506325	0.0
6	0.0	0.0	0.0	7.976349197147991	0.0
7	0.0	0.0	0.0	9.440225687399764	0.0
8	0.0	0.0	0.0	11.911966456050857	0.0
9	0.0	0.0	0.0	12.88698239715572	0.0
10	0.0	0.0	0.0	15.772225764689969	0.0
11	0.0	0.0	0.0	18.070450022220935	0.0
12	0.0	0.0	0.0	21.15355631557591	0.0
13	0.0	0.0	0.0	22.046644638958128	0.0
14	0.0	0.0	0.0	22.429231155681798	0.0
15	0.0	0.0	0.0	23.311885301335188	0.0
16	0.0	0.0	0.0	24.550074391822697	0.0
17	0.0	0.0	0.0	25.908449751705216	0.0
18	0.0	0.0	0.0	27.644387764960484	0.0
19	0.0	0.0	0.0	28.845864007883602	0.0
20	0.0	0.0	0.0	29.75943423569648	0.0
21	0.0	0.0	0.0	30.66643479605047	0.0
22	0.0	0.0	0.0	31.542132823217976	0.0
23	0.0	0.0	0.0	32.356771588120495	0.0
24	0.0	0.0	0.0	32.99518868471393	0.0
25	3.8645102699360424E-4	0.0	0.0	33.53506076942399	0.0
26	3.8645102699360424E-4	0.0	0.0	34.0544509497034	0.0
27	3.8645102699360424E-4	0.0	0.0	34.63335458813982	0.0
28	3.8645102699360424E-4	0.0	0.0	35.17013506463393	0.0
29	3.8645102699360424E-4	0.0	0.0	35.70575618804707	0.0
30	3.8645102699360424E-4	0.0	0.0	36.337990068208605	0.0
31	3.8645102699360424E-4	0.0	0.0	36.93351110080575	0.0
32	3.8645102699360424E-4	0.0	0.0	37.50932313102622	0.0
33	3.8645102699360424E-4	0.0	0.0	37.99625142503816	0.0
34	3.8645102699360424E-4	0.0	0.0	38.49361389677893	0.0
35	3.8645102699360424E-4	0.0	0.0	39.0273027650571	0.0
36	3.8645102699360424E-4	0.0	0.0	39.55326261279539	0.0
37	3.8645102699360424E-4	0.0	0.0	40.05989990918401	0.0
38	3.8645102699360424E-4	0.0	0.0	40.537939829575095	0.0
39	3.8645102699360424E-4	0.0	0.0	41.07124224682627	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCCAT	20	7.0231454E-4	45.0	37
TCGTTGC	20	7.0231454E-4	45.0	42
CTGTATC	20	7.0231454E-4	45.0	22
CCCTCGA	20	7.0231454E-4	45.0	41
ATTAGGC	20	7.0231454E-4	45.0	23
CTCGTCT	20	7.0231454E-4	45.0	22
ACTTGCG	20	7.0231454E-4	45.0	14
TCCGACT	20	7.0231454E-4	45.0	11
ATCAGGT	20	7.0231454E-4	45.0	25
CGTTGCC	20	7.0231454E-4	45.0	43
CCAATGG	20	7.0231454E-4	45.0	2
TAAGTTA	20	7.0231454E-4	45.0	44
TAACGTT	20	7.0231454E-4	45.0	17
AGTATAC	20	7.0231454E-4	45.0	20
GTGGACC	20	7.0231454E-4	45.0	14
TTGAGCG	20	7.0231454E-4	45.0	1
CTTGCGT	20	7.0231454E-4	45.0	15
TCTCGTC	20	7.0231454E-4	45.0	21
TCGCTCG	20	7.0231454E-4	45.0	22
GTTCCGA	20	7.0231454E-4	45.0	9
>>END_MODULE