FastQCFastQC Report
Sat 18 Jun 2016
SRR3554390_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554390_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330025
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCG24160.7320657525945005No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTC23730.7190364366335884No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22970.6960078781910461No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGC19860.6017725929853799No Hit
GCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC19280.584198166805545No Hit
CCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC11490.3481554427694872No Hit
CTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGCT8750.2651314294371639Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTTCGGAT7600.23028558442542232No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCT5390.16332096053329292No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG4860.147261571093099No Hit
GAACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCT4630.1402924020907507No Hit
TCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTTCTGC4260.12908113021740777No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTC3990.12089993182334673No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCCGTCTT3980.12059692447541853No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTTCGGATCGTATGCC3870.11726384364820848No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTTCGGATCGTA3840.11635482160442391No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA3580.10847663055829103No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAG207.025969E-445.0000041
CGAAAGG302.160783E-645.0000042
ACGGGTA302.160783E-645.0000045
CACCTCG207.025969E-445.00000438
CACTCGA253.8847415E-545.00000444
ACTACGG207.025969E-445.0000042
TTACGGG600.045.0000043
CGAACCC351.2084092E-745.034
GCGAACC351.2084092E-745.033
CGATGAA5450.044.1743119
GATGAAT6100.043.5245920
CCGATGA5400.041.66666818
CGATCGA650.041.5384641
ATGAATG6550.040.5343521
ATAGGGA1900.040.2631574
TTTGCGC451.9212166E-840.013
CGTTTTT10900.039.6330261
ACAGGGC1200.039.3750044
GTAGTTG403.44824E-739.3750041
GTTGATC1550.039.19354616
Produced by FastQC (version 0.11.5)