FastQCFastQC Report
Sat 18 Jun 2016
SRR3554389_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554389_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334537
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18320.547622535026021No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTC16020.47887079755004675No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGC15260.4561528321232031No Hit
GCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC14960.44718521419155427No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCG14630.4373208344667406No Hit
CCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC6840.20446168884159302No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG6590.19698867389855232No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTT6130.18323832640335747No Hit
CTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGCT5840.1745696290694303Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCTCTGTT5360.16022144037879218No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA3870.11568227131826972No Hit
TCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC3580.10701357398434252No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGG406.7902874E-945.0000042
CCGATCG207.026107E-445.00000440
CGCGCCT207.026107E-445.00000417
CGTAGCA207.026107E-445.00000431
TACGAAG453.8380676E-1045.0000041
GGACGTA207.026107E-445.0000049
CGTAAGG207.026107E-445.0000042
CGATCGA207.026107E-445.00000441
ACGACGG207.026107E-445.0000042
GCATAAG253.884857E-545.01
TTAACGG302.160872E-644.9999962
ACGAACG302.160872E-644.9999961
CTTAGAG1250.043.19999721
ACGAAGG750.042.0000042
CGATGAA3650.041.9178119
CCGATGA3600.041.87500418
TGGGTAC603.6379788E-1241.2499966
AGACACG1650.040.90909224
ACGGGTA556.002665E-1140.909095
TCTTAGA1500.040.50000420