##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554389_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	334537
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.98667113054759	33.0	31.0	34.0	30.0	34.0
2	32.00996900193402	33.0	31.0	34.0	30.0	34.0
3	32.07507689732376	33.0	31.0	34.0	30.0	34.0
4	35.678498940326485	37.0	35.0	37.0	33.0	37.0
5	35.7501173263346	37.0	35.0	37.0	35.0	37.0
6	35.75898331126303	37.0	35.0	37.0	35.0	37.0
7	35.87850073385007	37.0	35.0	37.0	35.0	37.0
8	35.84115957278268	37.0	35.0	37.0	35.0	37.0
9	37.62683649342225	39.0	38.0	39.0	35.0	39.0
10	37.319677644027415	39.0	37.0	39.0	34.0	39.0
11	37.27834888218643	39.0	37.0	39.0	34.0	39.0
12	37.20887973527592	39.0	37.0	39.0	34.0	39.0
13	37.162636718808386	39.0	37.0	39.0	33.0	39.0
14	38.37695083055088	40.0	38.0	41.0	34.0	41.0
15	38.444841676705416	40.0	38.0	41.0	34.0	41.0
16	38.52814785808446	40.0	38.0	41.0	34.0	41.0
17	38.42177696338521	40.0	38.0	41.0	34.0	41.0
18	38.411308764053004	40.0	38.0	41.0	34.0	41.0
19	38.35823242272155	40.0	37.0	41.0	34.0	41.0
20	38.206847075211414	40.0	37.0	41.0	34.0	41.0
21	38.16011681816959	40.0	37.0	41.0	34.0	41.0
22	38.16209268332053	40.0	37.0	41.0	34.0	41.0
23	38.07240753638611	40.0	37.0	41.0	34.0	41.0
24	38.04948331574684	40.0	36.0	41.0	34.0	41.0
25	37.925407354044545	40.0	36.0	41.0	33.0	41.0
26	37.90937624238873	40.0	36.0	41.0	33.0	41.0
27	37.822913459497755	40.0	36.0	41.0	33.0	41.0
28	37.66107485868529	40.0	36.0	41.0	33.0	41.0
29	37.57451343199706	40.0	36.0	41.0	33.0	41.0
30	37.477806640222155	40.0	36.0	41.0	33.0	41.0
31	37.50143930267803	40.0	36.0	41.0	33.0	41.0
32	37.369343301338866	40.0	35.0	41.0	33.0	41.0
33	37.26780296349881	40.0	35.0	41.0	32.0	41.0
34	37.11737715110735	40.0	35.0	41.0	32.0	41.0
35	37.0400942197724	40.0	35.0	41.0	32.0	41.0
36	36.929371041170334	40.0	35.0	41.0	31.0	41.0
37	36.86554551514481	40.0	35.0	41.0	31.0	41.0
38	36.80708860305437	40.0	35.0	41.0	31.0	41.0
39	36.670045465822916	39.0	35.0	41.0	31.0	41.0
40	36.644852437846936	39.0	35.0	41.0	31.0	41.0
41	36.520136786065514	39.0	35.0	41.0	30.0	41.0
42	36.43418216819067	39.0	35.0	41.0	30.0	41.0
43	36.33448915964453	39.0	35.0	41.0	30.0	41.0
44	36.325082128434225	39.0	35.0	41.0	30.0	41.0
45	36.26066473962521	39.0	35.0	41.0	30.0	41.0
46	36.19019121950636	39.0	35.0	41.0	30.0	41.0
47	36.06921506440244	39.0	35.0	41.0	30.0	41.0
48	36.03316524031722	39.0	35.0	41.0	30.0	41.0
49	35.899487949016105	38.0	35.0	41.0	29.0	41.0
50	35.80142106852157	38.0	35.0	41.0	29.0	41.0
51	35.01232748545004	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	4.0
10	8.0
11	13.0
12	7.0
13	12.0
14	18.0
15	24.0
16	34.0
17	63.0
18	107.0
19	183.0
20	374.0
21	648.0
22	1134.0
23	1602.0
24	2017.0
25	2733.0
26	3229.0
27	3701.0
28	3807.0
29	4197.0
30	4685.0
31	5862.0
32	7511.0
33	10140.0
34	18490.0
35	25917.0
36	23699.0
37	34178.0
38	58788.0
39	121190.0
40	156.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.35462445110705	18.71213049677614	22.81750598588497	13.115739066231837
2	32.88305927296532	26.3576226247022	23.644918200378434	17.114399901954044
3	31.629087365523095	25.856332782323033	26.364198877852075	16.150380974301797
4	27.85132885151717	24.738369746844146	29.269707087706294	18.14059431393239
5	26.881630432508214	29.03236413311532	24.721929113969455	19.364076320407012
6	24.53032101082989	35.785877197440044	24.929977849983707	14.753823941746354
7	76.89702484329088	6.202901323321486	12.583660402287341	4.316413431100297
8	77.23121807154367	7.875959908769434	9.747501771104542	5.145320248582369
9	71.37207543560204	7.505298367594617	11.981933239073705	9.14069295772964
10	38.571817168205605	26.4461031216278	19.448073008366787	15.534006701799802
11	30.360169428194787	26.183351916230492	24.929678929385986	18.526799726188734
12	26.781193111673744	22.36195099495721	29.719582587277344	21.137273306091704
13	26.383927637301703	24.049656689693517	31.16157555068647	18.40484012231831
14	21.38448064040749	28.79382549613346	29.273891976074395	20.547801887384654
15	19.45763846749388	24.458878987974426	34.90107222818403	21.18241031634767
16	21.99457758035733	24.42689448401821	30.505145918089777	23.073382017534684
17	21.461004313424226	25.11979242953694	28.79771146390384	24.62149179313499
18	24.758397426891495	21.88995537115476	29.567730923634755	23.78391627831899
19	24.37548014121009	24.697118704358562	27.5434406358639	23.383960518567452
20	26.574340058050378	23.91035969115524	28.621647231845802	20.893653018948577
21	24.36621360268072	27.62863300621456	27.96342407566278	20.041729315441938
22	22.350293091646066	23.96715460472235	28.945378239178325	24.73717406445326
23	21.545897763177166	26.608118085592924	28.551400891381224	23.294583259848686
24	22.123711278573072	24.727010764130725	29.089457967280154	24.059819990016052
25	21.8753082618664	27.5255054000006	26.92736528395962	23.671821054173382
26	20.588455088674795	26.202482834484677	29.327996604262008	23.88106547257852
27	24.20599216230193	24.998430666861964	27.625942720835067	23.16963445000105
28	21.12441972038967	26.76564924059222	29.17763954360803	22.932291495410073
29	21.875906103061844	25.742444034591095	29.596128380418307	22.785521481928754
30	23.620107790767538	25.836902943471124	28.167885764504376	22.37510350125696
31	23.36512852091099	25.90206763377444	25.600755671271042	25.132048174043533
32	24.59608354232865	26.95576274074318	27.201475472070353	21.24667824485782
33	23.859842110140285	23.80394395836634	27.814262697399688	24.521951234093688
34	22.213985299085003	25.373874937600327	28.725073758657487	23.687066004657183
35	23.97432869906767	25.045660121301978	28.0396488280818	22.940362351548558
36	23.606955284467787	27.204763598645293	26.858314625885924	22.329966491000995
37	22.87250737586575	25.728095845900455	28.841652791768922	22.557743986464875
38	22.273470498031607	25.147592045125055	27.01524793968978	25.56368951715356
39	23.577063224695625	23.18487940048485	29.157611863560685	24.080445511258844
40	24.546163802509138	22.383174357395443	29.416776021785335	23.653885818310084
41	21.6693519700362	23.975225460860834	28.922660273751482	25.432762295351484
42	24.12916956868747	24.073271416913524	28.678143224815194	23.119415789583815
43	23.29308865686008	24.309119768515888	28.785156798799534	23.6126347758245
44	22.29021005150402	23.604264999088294	28.948965286350987	25.156559663056704
45	21.855579502416774	22.94305263692805	28.228267725244145	26.97310013541103
46	23.211782254279793	24.868400206853053	27.477080263169697	24.442737275697457
47	21.429019809468013	24.70369495750844	30.85458409682636	23.01270113619719
48	22.36015747137088	23.75103501256961	29.235032298370584	24.653775217688924
49	22.668045687024154	22.128494008136617	31.87958282641382	23.323877478425405
50	21.354289660037605	23.237788346281576	30.542809913402703	24.865112080278116
51	21.46010755163106	23.09998595073191	28.215713060139834	27.224193437497195
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	180.0
1	417.0
2	654.0
3	1823.0
4	2992.0
5	2022.5
6	1053.0
7	1011.5
8	970.0
9	978.0
10	986.0
11	988.0
12	990.0
13	985.5
14	981.0
15	957.5
16	934.0
17	888.5
18	843.0
19	920.5
20	998.0
21	1379.5
22	1761.0
23	1716.5
24	1672.0
25	1760.5
26	2303.0
27	2757.0
28	3316.5
29	3876.0
30	4193.5
31	4511.0
32	4902.5
33	5294.0
34	6034.5
35	6775.0
36	7667.5
37	8560.0
38	9158.0
39	9756.0
40	11341.5
41	12927.0
42	14209.5
43	15492.0
44	17350.0
45	19208.0
46	25410.0
47	31612.0
48	31576.5
49	31541.0
50	31538.5
51	31536.0
52	29096.5
53	26657.0
54	24268.5
55	21880.0
56	19766.0
57	17652.0
58	16520.5
59	15389.0
60	14673.5
61	13958.0
62	12583.0
63	11208.0
64	9893.0
65	8578.0
66	7228.0
67	5878.0
68	4908.5
69	3939.0
70	3557.5
71	3176.0
72	2735.5
73	2295.0
74	1928.5
75	1181.0
76	800.0
77	619.0
78	438.0
79	340.0
80	242.0
81	165.0
82	88.0
83	70.0
84	52.0
85	39.0
86	26.0
87	16.5
88	7.0
89	4.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	334537.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.81430370381575
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.21698044286772	27.32246458003886
2	8.375840018684148	6.1670143644482405
3	3.202908641932631	3.537385544390518
4	1.9568281198733466	2.8815705880473668
5	1.4853989621812078	2.7341964257535856
6	1.2088292126184113	2.670132345562719
7	1.090776920372832	2.8109334973802804
8	0.8981077310056722	2.6450568614390146
9	0.8070699530235497	2.674054652474994
>10	6.6678567599367105	40.09483797541073
>50	0.056892969320575734	1.4739120145872073
>100	0.024382701137389603	1.6986192665066129
>500	0.004063783522898267	0.9203721796326126
>1k	0.004063783522898267	2.369449704327262
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1832	0.547622535026021	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTC	1602	0.47887079755004675	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGC	1526	0.4561528321232031	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC	1496	0.44718521419155427	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCG	1463	0.4373208344667406	No Hit
CCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC	684	0.20446168884159302	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	659	0.19698867389855232	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTT	613	0.18323832640335747	No Hit
CTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGCT	584	0.1745696290694303	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCTCTGTT	536	0.16022144037879218	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	387	0.11568227131826972	No Hit
TCTGTCTCTTATACACATCTGACGCAGCTCTGTTCGTATGCCGTCTTCTGC	358	0.10701357398434252	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9892059772162723E-4	0.0	0.0	0.17875451743753307	0.0
2	2.9892059772162723E-4	0.0	0.0	1.0372544740940464	0.0
3	2.9892059772162723E-4	0.0	0.0	1.418378236189121	0.0
4	2.9892059772162723E-4	0.0	0.0	1.8829008450485298	0.0
5	2.9892059772162723E-4	0.0	0.0	3.326986252641711	0.0
6	2.9892059772162723E-4	0.011060062115700207	0.0	4.171138020607586	0.0
7	2.9892059772162723E-4	0.011060062115700207	0.0	4.87539494883974	0.0
8	2.9892059772162723E-4	0.011060062115700207	0.0	6.0591205158173835	0.0
9	2.9892059772162723E-4	0.011060062115700207	0.0	6.537991313367431	0.0
10	2.9892059772162723E-4	0.011060062115700207	0.0	7.972212341235798	0.0
11	2.9892059772162723E-4	0.011060062115700207	0.0	9.268332052956772	0.0
12	2.9892059772162723E-4	0.011060062115700207	0.0	10.808370972418597	0.0
13	2.9892059772162723E-4	0.011060062115700207	0.0	11.416076547586664	0.0
14	2.9892059772162723E-4	0.011060062115700207	0.0	11.71290470112424	0.0
15	2.9892059772162723E-4	0.011060062115700207	0.0	12.133784902716291	0.0
16	2.9892059772162723E-4	0.011060062115700207	0.0	12.854183543225414	0.0
17	2.9892059772162723E-4	0.011060062115700207	0.0	13.701025596570783	0.0
18	2.9892059772162723E-4	0.011060062115700207	0.0	14.689257092638483	0.0
19	2.9892059772162723E-4	0.011060062115700207	0.0	15.322968759808331	0.0
20	2.9892059772162723E-4	0.011060062115700207	0.0	15.810807175290028	0.0
21	2.9892059772162723E-4	0.011060062115700207	0.0	16.351853457166172	0.0
22	2.9892059772162723E-4	0.011060062115700207	0.0	16.891106215455988	0.0
23	2.9892059772162723E-4	0.011060062115700207	0.0	17.46473484248379	0.0
24	2.9892059772162723E-4	0.011060062115700207	0.0	17.901158915157367	0.0
25	2.9892059772162723E-4	0.011060062115700207	0.0	18.291848136379535	0.0
26	2.9892059772162723E-4	0.011060062115700207	0.0	18.691206054935627	0.0
27	2.9892059772162723E-4	0.011060062115700207	0.0	19.114776541907172	0.0
28	2.9892059772162723E-4	0.011060062115700207	0.0	19.495601383404527	0.0
29	2.9892059772162723E-4	0.011060062115700207	0.0	19.87821974848821	0.0
30	2.9892059772162723E-4	0.011060062115700207	0.0	20.31554058295495	0.0
31	5.978411954432545E-4	0.011060062115700207	0.0	20.747779767260422	0.0
32	5.978411954432545E-4	0.011060062115700207	0.0	21.148333368207403	0.0
33	5.978411954432545E-4	0.011060062115700207	0.0	21.576985505340218	0.0
34	5.978411954432545E-4	0.011060062115700207	0.0	21.999360309920874	0.0
35	5.978411954432545E-4	0.011060062115700207	0.0	22.429208129444575	0.0
36	5.978411954432545E-4	0.011060062115700207	0.0	22.82677252441434	0.0
37	8.967617931648816E-4	0.011060062115700207	0.0	23.22553260177499	0.0
38	8.967617931648816E-4	0.011060062115700207	0.0	23.647309565160207	0.0
39	8.967617931648816E-4	0.011060062115700207	0.0	24.08881528799505	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGG	40	6.7902874E-9	45.000004	2
CCGATCG	20	7.026107E-4	45.000004	40
CGCGCCT	20	7.026107E-4	45.000004	17
CGTAGCA	20	7.026107E-4	45.000004	31
TACGAAG	45	3.8380676E-10	45.000004	1
GGACGTA	20	7.026107E-4	45.000004	9
CGTAAGG	20	7.026107E-4	45.000004	2
CGATCGA	20	7.026107E-4	45.000004	41
ACGACGG	20	7.026107E-4	45.000004	2
GCATAAG	25	3.884857E-5	45.0	1
TTAACGG	30	2.160872E-6	44.999996	2
ACGAACG	30	2.160872E-6	44.999996	1
CTTAGAG	125	0.0	43.199997	21
ACGAAGG	75	0.0	42.000004	2
CGATGAA	365	0.0	41.91781	19
CCGATGA	360	0.0	41.875004	18
TGGGTAC	60	3.6379788E-12	41.249996	6
AGACACG	165	0.0	40.909092	24
ACGGGTA	55	6.002665E-11	40.90909	5
TCTTAGA	150	0.0	40.500004	20
>>END_MODULE