SRR3554388_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554388_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	330832
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2014	0.6087681965468879	No Hit
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	1071	0.32372926440005806	Illumina PCR Primer Index 7 (96% over 27bp)
GAATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC	998	0.3016636842868888	Illumina PCR Primer Index 7 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG	987	0.298338733858877	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC	933	0.2820162499395463	No Hit
CTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGCT	640	0.19345166126614113	Illumina PCR Primer Index 7 (96% over 28bp)
CCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	587	0.17743144556753881	Illumina PCR Primer Index 7 (96% over 27bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	483	0.1459955506117909	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAGCATCT	388	0.11728006964259806	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCC	360	0.10881655946220438	No Hit
TCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	354	0.1070029501378343	Illumina PCR Primer Index 7 (96% over 27bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGAT	20	7.0259935E-4	45.000004	34
TCGATTG	20	7.0259935E-4	45.000004	1
GGTATTA	20	7.0259935E-4	45.000004	8
CATCGTT	20	7.0259935E-4	45.000004	36
ATAACGC	20	7.0259935E-4	45.000004	11
CAGTCCG	20	7.0259935E-4	45.000004	9
CGCACGG	35	1.2084274E-7	45.000004	2
GCCAACG	20	7.0259935E-4	45.000004	1
TGCGTAG	30	2.1607975E-6	45.000004	1
CCCGAAT	20	7.0259935E-4	45.000004	37
CAACACG	25	3.884763E-5	45.0	22
AATTGCG	25	3.884763E-5	45.0	1
CGAGGGT	25	3.884763E-5	45.0	4
CGATGCG	50	2.1827873E-11	45.0	10
ATTGCGG	65	0.0	44.999996	2
CGATGAA	320	0.0	43.593754	19
CGTTCAG	100	0.0	42.75	38
TATGGGC	75	0.0	42.0	4
CTCGAAT	60	3.6379788E-12	41.250004	43
GTGGCGT	100	0.0	40.5	34