##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3554388_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 330832 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.031061082362044 33.0 31.0 34.0 30.0 34.0 2 32.07837814963486 33.0 31.0 34.0 30.0 34.0 3 32.149208661798134 34.0 31.0 34.0 30.0 34.0 4 35.742663950282925 37.0 35.0 37.0 33.0 37.0 5 35.80683851622576 37.0 35.0 37.0 35.0 37.0 6 35.82047383566282 37.0 35.0 37.0 35.0 37.0 7 35.9467554529187 37.0 35.0 37.0 35.0 37.0 8 35.87918036949267 37.0 35.0 37.0 35.0 37.0 9 37.67023141654979 39.0 38.0 39.0 35.0 39.0 10 37.390306862697685 39.0 37.0 39.0 34.0 39.0 11 37.33266431300479 39.0 37.0 39.0 34.0 39.0 12 37.23469615998452 39.0 37.0 39.0 34.0 39.0 13 37.19252672051071 39.0 37.0 39.0 34.0 39.0 14 38.392359263916426 40.0 38.0 41.0 34.0 41.0 15 38.477215021521495 40.0 38.0 41.0 34.0 41.0 16 38.527968878463994 40.0 38.0 41.0 34.0 41.0 17 38.45139527010688 40.0 38.0 41.0 34.0 41.0 18 38.43387882671568 40.0 38.0 41.0 34.0 41.0 19 38.345761595008945 40.0 37.0 41.0 34.0 41.0 20 38.19150505392465 40.0 37.0 41.0 34.0 41.0 21 38.1712319243604 40.0 37.0 41.0 34.0 41.0 22 38.1862969724815 40.0 37.0 41.0 34.0 41.0 23 38.07720837162064 40.0 36.0 41.0 34.0 41.0 24 38.03354270445423 40.0 36.0 41.0 34.0 41.0 25 37.93132163756831 40.0 36.0 41.0 34.0 41.0 26 37.89819304057649 40.0 36.0 41.0 34.0 41.0 27 37.836696571069304 40.0 36.0 41.0 33.0 41.0 28 37.708933839531845 40.0 36.0 41.0 33.0 41.0 29 37.69605419064661 40.0 36.0 41.0 33.0 41.0 30 37.54088177685351 40.0 35.0 41.0 33.0 41.0 31 37.477840716738406 40.0 35.0 41.0 33.0 41.0 32 37.31793175992649 40.0 35.0 41.0 33.0 41.0 33 37.23362915316535 40.0 35.0 41.0 33.0 41.0 34 37.02397893795038 40.0 35.0 41.0 32.0 41.0 35 36.953175629926974 40.0 35.0 41.0 32.0 41.0 36 36.86500398994051 40.0 35.0 41.0 31.0 41.0 37 36.74139744643807 40.0 35.0 41.0 31.0 41.0 38 36.65169330657252 39.0 35.0 41.0 31.0 41.0 39 36.56546827392755 39.0 35.0 41.0 31.0 41.0 40 36.475177733713785 39.0 35.0 41.0 30.0 41.0 41 36.39608018571359 39.0 35.0 41.0 30.0 41.0 42 36.35378379358708 39.0 35.0 41.0 30.0 41.0 43 36.25443125211588 39.0 35.0 41.0 30.0 41.0 44 36.16985962663829 39.0 35.0 41.0 30.0 41.0 45 36.08831672873241 39.0 35.0 41.0 30.0 41.0 46 35.99595262852445 38.0 35.0 41.0 30.0 41.0 47 35.872938530734636 38.0 35.0 41.0 30.0 41.0 48 35.843914736180295 38.0 35.0 41.0 30.0 41.0 49 35.75913454563041 38.0 35.0 41.0 29.0 41.0 50 35.66800369976302 38.0 35.0 40.0 29.0 41.0 51 34.83658170914543 37.0 34.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 1.0 10 10.0 11 8.0 12 6.0 13 11.0 14 19.0 15 22.0 16 35.0 17 65.0 18 92.0 19 171.0 20 384.0 21 734.0 22 1090.0 23 1456.0 24 1990.0 25 2653.0 26 3259.0 27 3659.0 28 3812.0 29 4098.0 30 4718.0 31 5670.0 32 7430.0 33 10076.0 34 18320.0 35 26993.0 36 24162.0 37 35194.0 38 58182.0 39 116377.0 40 133.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.19977511244378 19.140228272960293 22.703668327126756 13.95632828746917 2 35.09757218165111 24.931082845673938 23.211781206171107 16.759563766503845 3 32.70058519127533 25.06408086279441 26.073052183585627 16.162281762344634 4 28.811904531605165 24.14881269042898 28.33643662039948 18.702846157566377 5 26.781266624752142 28.9370435749867 24.759696764520967 19.521993035740195 6 24.84130918411762 35.84115200464284 24.54478405958311 14.77275475165643 7 76.77280311457174 6.423199690477342 12.18775692798762 4.616240266963293 8 77.35466943947381 7.293127629733521 10.174952846157566 5.1772500846351015 9 71.35948154954781 7.6981670455095035 12.527808676307009 8.414542728635682 10 39.40005803549838 25.65864245296707 19.7033539681772 15.237945543357354 11 32.02652705905112 25.408062097983265 24.580149441408327 17.98526140155729 12 27.19930357401944 22.868706775644434 28.998404023794556 20.933585626541568 13 25.940356434685885 23.831733326884944 30.411205687478844 19.81670455095033 14 21.33711370121391 27.7938651641921 29.62228563137786 21.246735503216133 15 20.623458432074283 23.425787106446776 33.940489432703004 22.010265028775937 16 23.787299898437876 23.673647047444017 30.344404410697877 22.194648643420226 17 22.849059341297092 23.97893795037965 29.379866518353726 23.792136189969533 18 25.681614837742416 21.938627460463316 29.603545001692698 22.77621270010156 19 24.703474875465492 25.14660008705325 27.260361754606567 22.889563282874693 20 27.798096919282294 23.908509454949943 28.019659525076175 20.27373410069159 21 24.48825990230691 26.831745417613774 28.29442133771824 20.385573342361077 22 23.451177636987957 23.636165788073704 28.599409972433136 24.3132466025052 23 23.55697151424288 26.210281955796294 27.740665957343907 22.492080572616917 24 22.663164385549162 24.60191275330077 29.049185084876918 23.685737776273154 25 22.22366639261015 26.638898292789086 27.753965759055955 23.38346955554481 26 21.224972191323694 25.41864148570876 29.720825071335298 23.635561251632247 27 24.219241185858685 24.542365913817285 28.434673792136188 22.803719108187842 28 20.227184794699422 28.21704067321178 28.84938579097548 22.706388741113315 29 23.78881123954152 25.191033515500315 28.431651109928907 22.58850413502926 30 23.94629298254099 25.86085989263433 27.956787735164674 22.23605938966001 31 23.88372346085022 27.12917734681047 25.774108913285293 23.212990279054022 32 25.98055810804275 26.65189582628041 26.10176766455482 21.26577840112202 33 24.45893988489626 25.672849059341296 24.930176041011755 24.93803501475069 34 22.003010591478454 27.95588093050249 27.50822169560381 22.532886782415243 35 24.286949267301832 25.56493930454128 27.203837597330367 22.94427383082652 36 23.17550901968371 30.07145620738018 24.722820041592108 22.030214731344007 37 24.337428060163464 27.636081152971904 26.056427431445567 21.970063355419065 38 23.09601247763215 27.368573777627315 25.30740678048073 24.228006964259805 39 24.473146491270494 25.44372974802921 25.496324418435947 24.58679934226435 40 23.42699617932969 24.257326981670456 28.53744498718383 23.778231851816027 41 20.631619674033953 26.15285099385791 27.25612999951637 25.959399332591772 42 23.312134255452918 25.130277603133916 27.210487498186392 24.347100643226774 43 23.28462784736664 24.984282052522126 27.206558011316922 24.524532088794313 44 23.440295981041736 25.50660153794071 27.00222469410456 24.050877786912995 45 23.216617497702764 24.652089277941673 26.856833679934226 25.27445954442134 46 23.17097499637278 26.135621705276396 26.548217826570585 24.145185471780238 47 21.131571311118634 26.33209604874982 29.59991778304396 22.936414857087588 48 22.04049185084877 25.45310006287179 28.558906030855542 23.9475020554239 49 23.20392223243217 23.665183537263626 29.162535667650047 23.968358562654156 50 21.52875175315568 24.66538907965372 28.467621028195577 25.338238138995017 51 21.131571311118634 24.45924215311699 26.90187164482275 27.507314890941625 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 231.0 1 512.5 2 794.0 3 2206.0 4 3618.0 5 2476.5 6 1335.0 7 1258.5 8 1182.0 9 1141.5 10 1101.0 11 1077.0 12 1053.0 13 1104.0 14 1155.0 15 1116.5 16 1078.0 17 1039.5 18 1001.0 19 1041.5 20 1082.0 21 1448.0 22 1814.0 23 1734.0 24 1654.0 25 1756.0 26 2242.5 27 2627.0 28 3150.0 29 3673.0 30 4174.0 31 4675.0 32 4819.5 33 4964.0 34 5754.5 35 6545.0 36 7227.5 37 7910.0 38 8707.5 39 9505.0 40 10818.0 41 12131.0 42 13465.0 43 14799.0 44 16571.5 45 18344.0 46 22734.0 47 27124.0 48 27798.5 49 28473.0 50 29323.5 51 30174.0 52 28003.0 53 25832.0 54 23685.5 55 21539.0 56 19798.5 57 18058.0 58 17145.0 59 16232.0 60 15397.0 61 14562.0 62 13461.0 63 12360.0 64 10999.5 65 9639.0 66 8113.0 67 6587.0 68 5688.5 69 4790.0 70 4373.0 71 3956.0 72 3304.5 73 2653.0 74 2263.0 75 1596.0 76 1319.0 77 1023.0 78 727.0 79 600.0 80 473.0 81 359.0 82 245.0 83 152.5 84 60.0 85 38.0 86 16.0 87 12.5 88 9.0 89 5.0 90 1.0 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 330832.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.94036190853751 #Duplication Level Percentage of deduplicated Percentage of total 1 68.55951430230832 21.898156990892815 2 9.932877471788029 6.3451940248413745 3 3.827775379800407 3.6678159280624385 4 2.4366321470178742 3.1130765045491073 5 1.838194653034907 2.935630123813673 6 1.4831998757856306 2.8424364489174554 7 1.3098256395361214 2.928541347470572 8 1.1076160016114163 2.830212475772472 9 1.021953565691691 2.9377410067721677 >10 8.357903992906685 44.26144048803739 >50 0.07968446113211586 1.6906162375906846 >100 0.03785011903775502 2.2489586344615264 >500 0.004980278820757241 1.3187061168260046 >1k 0.001992111528302896 0.9814736719923339 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2014 0.6087681965468879 No Hit GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC 1071 0.32372926440005806 Illumina PCR Primer Index 7 (96% over 27bp) GAATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC 998 0.3016636842868888 Illumina PCR Primer Index 7 (95% over 24bp) GAATGATACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG 987 0.298338733858877 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC 933 0.2820162499395463 No Hit CTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGCT 640 0.19345166126614113 Illumina PCR Primer Index 7 (96% over 28bp) CCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC 587 0.17743144556753881 Illumina PCR Primer Index 7 (96% over 27bp) CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG 483 0.1459955506117909 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAGCATCT 388 0.11728006964259806 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCC 360 0.10881655946220438 No Hit TCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC 354 0.1070029501378343 Illumina PCR Primer Index 7 (96% over 27bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.19768341635633796 0.0 2 0.0 0.0 0.0 0.8962252744595445 0.0 3 0.0 0.0 0.0 1.1894254485660396 0.0 4 0.0 0.0 0.0 1.5612153600619045 0.0 5 0.0 0.0 0.0 2.6351743483097163 0.0 6 0.0 0.0 0.0 3.188929728684045 0.0 7 0.0 0.0 0.0 3.8203680417855588 0.0 8 0.0 0.0 0.0 4.9330173622865985 0.0 9 0.0 0.0 0.0 5.3870242298205735 0.0 10 0.0 0.0 0.0 6.66108478019055 0.0 11 0.0 0.0 0.0 7.948747400493302 0.0 12 0.0 0.0 0.0 9.253337041156842 0.0 13 0.0 0.0 0.0 9.704018958262804 0.0 14 0.0 0.0 0.0 9.901702374619141 0.0 15 0.0 0.0 0.0 10.30764859505731 0.0 16 0.0 0.0 0.0 10.989867969241185 0.0 17 0.0 0.0 0.0 11.747654398607148 0.0 18 0.0 0.0 0.0 12.577985200947913 0.0 19 0.0 0.0 0.0 13.14685399235866 0.0 20 0.0 0.0 0.0 13.594815495478068 0.0 21 0.0 0.0 0.0 14.114716835130821 0.0 22 0.0 0.0 0.0 14.613459399332593 0.0 23 0.0 0.0 0.0 15.101924844029599 0.0 24 0.0 0.0 0.0 15.514823233544519 0.0 25 0.0 0.0 0.0 15.882381389950186 0.0 26 0.0 0.0 0.0 16.231501184891425 0.0 27 0.0 0.0 0.0 16.621124921410264 0.0 28 0.0 0.0 0.0 16.991403491802487 0.0 29 0.0 0.0 0.0 17.37044784059583 0.0 30 0.0 0.0 0.0 17.808434492431203 0.0 31 0.0 0.0 0.0 18.20168544759878 0.0 32 0.0 0.0 0.0 18.597656816752913 0.0 33 0.0 0.0 0.0 18.996046331672872 0.0 34 0.0 0.0 0.0 19.363302219857815 0.0 35 0.0 0.0 0.0 19.7417420322097 0.0 36 3.022682207283455E-4 0.0 0.0 20.1256226725347 0.0 37 3.022682207283455E-4 0.0 0.0 20.517966823040094 0.0 38 3.022682207283455E-4 0.0 0.0 20.9094041688833 0.0 39 3.022682207283455E-4 0.0 0.0 21.321093485515306 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACGAT 20 7.0259935E-4 45.000004 34 TCGATTG 20 7.0259935E-4 45.000004 1 GGTATTA 20 7.0259935E-4 45.000004 8 CATCGTT 20 7.0259935E-4 45.000004 36 ATAACGC 20 7.0259935E-4 45.000004 11 CAGTCCG 20 7.0259935E-4 45.000004 9 CGCACGG 35 1.2084274E-7 45.000004 2 GCCAACG 20 7.0259935E-4 45.000004 1 TGCGTAG 30 2.1607975E-6 45.000004 1 CCCGAAT 20 7.0259935E-4 45.000004 37 CAACACG 25 3.884763E-5 45.0 22 AATTGCG 25 3.884763E-5 45.0 1 CGAGGGT 25 3.884763E-5 45.0 4 CGATGCG 50 2.1827873E-11 45.0 10 ATTGCGG 65 0.0 44.999996 2 CGATGAA 320 0.0 43.593754 19 CGTTCAG 100 0.0 42.75 38 TATGGGC 75 0.0 42.0 4 CTCGAAT 60 3.6379788E-12 41.250004 43 GTGGCGT 100 0.0 40.5 34 >>END_MODULE