SRR3554387_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554387_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	211599
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCG	1918	0.9064315048747866	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGC	1842	0.8705145109381425	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTC	1638	0.7741057377397813	No Hit
GCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	1423	0.6724984522611165	TruSeq Adapter, Index 27 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1184	0.5595489581708798	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCACTTTGT	643	0.3038766723850302	No Hit
CCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	507	0.23960415691945616	TruSeq Adapter, Index 27 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGCT	436	0.20605012311022264	TruSeq Adapter, Index 20 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCT	402	0.18998199424382914	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTA	373	0.17627682550484644	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTC	304	0.14366797574657725	No Hit
GGGGAGGGGGAAATACTGATTTATCAATGATTTTATGAAGAAAGATAATAT	301	0.14225019967013078	No Hit
TCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	280	0.13232576713500538	TruSeq Adapter, Index 27 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	269	0.12712725485470158	No Hit
ACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	232	0.10964134991186159	TruSeq Adapter, Index 20 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCT	232	0.10964134991186159	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCC	226	0.10680579775896862	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTA	30	2.1570795E-6	45.000004	5
CGTAAGG	30	2.1570795E-6	45.000004	2
ACACGGG	25	3.8799844E-5	45.0	3
CGAACGG	25	3.8799844E-5	45.0	2
CACGGGC	20	7.0202263E-4	45.0	4
ATAGCTA	20	7.0202263E-4	45.0	27
GTAGGGA	90	0.0	45.0	4
TATGCGG	20	7.0202263E-4	45.0	2
GTCAGCG	20	7.0202263E-4	45.0	35
ACGGCTA	20	7.0202263E-4	45.0	30
CCTCCGC	20	7.0202263E-4	45.0	45
TACTGGC	20	7.0202263E-4	45.0	13
TCTCGTA	25	3.8799844E-5	45.0	28
TATTACG	20	7.0202263E-4	45.0	1
CGCAGTA	20	7.0202263E-4	45.0	37
GACGGGT	20	7.0202263E-4	45.0	4
CATACGG	20	7.0202263E-4	45.0	2
CGTACGG	25	3.8799844E-5	45.0	31
GCTAGCA	20	7.0202263E-4	45.0	30
TCGTACG	25	3.8799844E-5	45.0	30

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content