##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554387_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	211599
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.93227283682815	33.0	31.0	34.0	30.0	34.0
2	32.028076692233896	33.0	31.0	34.0	30.0	34.0
3	32.09267529619705	33.0	31.0	34.0	30.0	34.0
4	35.705773656775314	37.0	35.0	37.0	33.0	37.0
5	35.75854328234065	37.0	35.0	37.0	35.0	37.0
6	35.76202628556846	37.0	35.0	37.0	35.0	37.0
7	35.911081810405534	37.0	35.0	37.0	35.0	37.0
8	35.81985737172671	37.0	35.0	37.0	35.0	37.0
9	37.62649161858043	39.0	37.0	39.0	35.0	39.0
10	37.30762905306736	39.0	37.0	39.0	34.0	39.0
11	37.24508149849479	39.0	37.0	39.0	34.0	39.0
12	37.12877187510338	39.0	37.0	39.0	34.0	39.0
13	37.037367851454874	39.0	37.0	39.0	33.0	39.0
14	38.187685196999986	40.0	37.0	41.0	33.0	41.0
15	38.25064863255498	40.0	37.0	41.0	33.0	41.0
16	38.35014343167973	40.0	37.0	41.0	34.0	41.0
17	38.239566349557414	40.0	37.0	41.0	34.0	41.0
18	38.23348881610972	40.0	37.0	41.0	34.0	41.0
19	38.14173507436236	40.0	37.0	41.0	34.0	41.0
20	37.900273630782756	40.0	36.0	41.0	33.0	41.0
21	37.92002325152765	40.0	36.0	41.0	33.0	41.0
22	37.89205525545962	40.0	36.0	41.0	33.0	41.0
23	37.75232869720556	40.0	35.0	41.0	33.0	41.0
24	37.726733113105446	40.0	35.0	41.0	33.0	41.0
25	37.692583613344105	40.0	35.0	41.0	33.0	41.0
26	37.6270067438882	40.0	35.0	41.0	33.0	41.0
27	37.515139485536324	40.0	35.0	41.0	33.0	41.0
28	37.410209878118515	40.0	35.0	41.0	33.0	41.0
29	37.40175048086238	40.0	35.0	41.0	33.0	41.0
30	37.28743992173876	40.0	35.0	41.0	32.0	41.0
31	37.23094154509237	40.0	35.0	41.0	32.0	41.0
32	36.92069905812409	40.0	35.0	41.0	31.0	41.0
33	36.799096404047276	40.0	35.0	41.0	31.0	41.0
34	36.628018090822735	40.0	35.0	41.0	31.0	41.0
35	36.48591912060076	39.0	35.0	41.0	30.0	41.0
36	36.401476377487604	39.0	35.0	41.0	30.0	41.0
37	36.35710943813534	39.0	35.0	41.0	30.0	41.0
38	36.17055373607626	39.0	35.0	41.0	30.0	41.0
39	36.1017396112458	39.0	35.0	41.0	29.0	41.0
40	36.039395271244196	39.0	35.0	41.0	29.0	41.0
41	35.99798203205119	39.0	35.0	41.0	29.0	41.0
42	35.93645055033341	39.0	35.0	41.0	29.0	41.0
43	35.83448409491538	39.0	35.0	41.0	29.0	41.0
44	35.81450762999825	39.0	35.0	41.0	29.0	41.0
45	35.69680858605192	38.0	35.0	41.0	28.0	41.0
46	35.62245568268281	38.0	35.0	41.0	28.0	41.0
47	35.458007835575785	38.0	35.0	41.0	28.0	41.0
48	35.49039456708208	38.0	35.0	40.0	28.0	41.0
49	35.41966644454841	38.0	35.0	40.0	27.0	41.0
50	35.307529808742004	38.0	35.0	40.0	27.0	41.0
51	34.582275908676316	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	7.0
11	8.0
12	4.0
13	9.0
14	12.0
15	13.0
16	29.0
17	33.0
18	72.0
19	134.0
20	306.0
21	561.0
22	929.0
23	1231.0
24	1727.0
25	2119.0
26	2654.0
27	2747.0
28	2841.0
29	2906.0
30	3128.0
31	3971.0
32	4957.0
33	6768.0
34	12406.0
35	17902.0
36	15287.0
37	21655.0
38	37167.0
39	69941.0
40	71.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.64058903870056	17.6049981332615	20.644237449137286	13.110175378900657
2	33.908005236319646	25.982636968983787	22.187250412336542	17.922107382360032
3	30.930202883756543	27.178767385479137	25.629138133923128	16.261891596841195
4	27.02895571340129	23.070997499988184	31.3843638202449	18.515682966365627
5	28.441533277567476	26.949560253120293	25.319590357232315	19.289316112079923
6	24.130548821119195	34.85791520753879	26.096059055099502	14.915476916242515
7	73.82549066867045	5.463636406599275	16.20848869796171	4.502384226768558
8	73.21631954782394	9.583221092727282	11.44334330502507	5.7571160544236974
9	68.64257392520759	5.974980978170975	13.590801468816016	11.791643627805424
10	38.74640239320602	25.243503041129685	20.635258200653123	15.374836365011177
11	31.337577209722163	24.039811152226616	26.06864871762154	18.553962920429683
12	24.86637460479492	22.137628249660914	30.60836771440319	22.38762943114098
13	25.529893808571874	25.05871955916616	32.25298796308111	17.158398669180855
14	20.26096531647125	29.77660574955458	29.81110496741478	20.15132396655939
15	18.531278503206536	23.504364387355327	35.8139688750892	22.150388234348934
16	21.060118431561587	24.089433314902244	30.537951502606347	24.312496750929824
17	20.24158904342648	26.01430063469109	30.385304278375607	23.35880604350682
18	24.758623622984985	20.73970103828468	31.064419018993473	23.43725631973686
19	21.920708509964605	24.935373040515316	28.55873609988705	24.585182349633033
20	26.5492748075369	23.862589142670807	30.77282973927098	18.815306310521315
21	23.08281230062524	28.475559903402193	29.431613570952603	19.010014225019965
22	22.762394907348334	21.59462001238191	31.45525262406722	24.187732456202536
23	21.589421500101608	26.648991724913635	29.622540749247396	22.13904602573736
24	21.377700272685598	24.62677044787546	30.705721671652515	23.289807607786425
25	20.050189273106206	28.93870008837471	28.286995685234807	22.72411495328428
26	20.4424406542564	23.92922461826379	31.53181253219533	24.096522195284475
27	23.76523518542148	24.273744204840288	28.367336329566772	23.593684280171455
28	17.48448716676355	28.002022693869066	30.703831303550583	23.809658835816805
29	20.135255837692995	23.4018119178257	30.89570366589634	25.567228578584967
30	20.550664228091815	25.230270464416183	31.03228276126069	23.186782546231317
31	22.56910476892613	25.104088393612443	27.071488995694686	25.25531784176674
32	23.36494973983809	24.70711109220743	28.212798737234106	23.71514043072037
33	22.63290469236622	23.0572923312492	27.97508494841658	26.334718027967995
34	19.74158668046635	24.061550385398796	31.43304079886956	24.76382213526529
35	20.234027571018768	23.9528542195379	30.028497299136575	25.78462091030676
36	22.308233970859977	28.39663703514667	26.28982178554719	23.005307208446165
37	20.56058866062694	24.697186659672305	28.91034456684578	25.831880112854975
38	20.799720225520915	27.032263857579668	27.731227463267782	24.43678845363163
39	22.753415658864174	23.628183498031653	28.614501958893946	25.00389888421023
40	22.504832253460556	21.91834554983719	31.680206428196733	23.896615768505523
41	20.794049121215128	23.734989295790623	28.26100312383329	27.20995845916096
42	22.76806601165412	22.62959654818785	28.75486179046215	25.847475649695888
43	24.20852650532375	22.951431717541197	26.559199240072022	26.28084253706303
44	21.94008478300937	22.59793288248054	29.169797588835483	26.2921847456746
45	21.2028412232572	22.224112590324154	29.081895472095802	27.49115071432285
46	23.643306442847084	23.010978312751952	29.33473220572876	24.010983038672208
47	19.070978596307167	25.388116200927225	32.94580787243796	22.595097330327647
48	21.49773864715807	24.32242118346495	29.289835963307954	24.890004206069026
49	22.66315058199708	21.43299353966701	32.59561718155568	23.308238696780233
50	21.905585565149174	22.708992008468847	30.569615168313653	24.81580725806833
51	21.768533877759346	20.66550409028398	28.2633660839607	29.302595947995975
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	118.0
1	249.5
2	381.0
3	1345.0
4	2309.0
5	1601.5
6	894.0
7	890.0
8	886.0
9	841.0
10	796.0
11	779.5
12	763.0
13	784.5
14	806.0
15	777.5
16	749.0
17	743.0
18	737.0
19	731.0
20	725.0
21	737.5
22	750.0
23	823.0
24	896.0
25	941.0
26	1209.5
27	1433.0
28	1603.5
29	1774.0
30	2121.5
31	2469.0
32	2953.5
33	3438.0
34	3680.0
35	3922.0
36	4640.5
37	5359.0
38	5800.0
39	6241.0
40	6838.5
41	7436.0
42	8165.5
43	8895.0
44	10673.5
45	12452.0
46	17442.0
47	22432.0
48	21841.5
49	21251.0
50	21693.5
51	22136.0
52	20010.0
53	17884.0
54	16122.5
55	14361.0
56	12801.5
57	11242.0
58	10139.0
59	9036.0
60	8158.0
61	7280.0
62	6611.5
63	5943.0
64	5200.0
65	4457.0
66	3788.5
67	3120.0
68	2651.5
69	2183.0
70	1897.5
71	1612.0
72	1456.0
73	1300.0
74	1154.0
75	801.5
76	595.0
77	447.0
78	299.0
79	230.0
80	161.0
81	113.5
82	66.0
83	38.5
84	11.0
85	6.5
86	2.0
87	3.0
88	4.0
89	2.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	211599.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.59847636330985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.21503808542195	27.903723552568778
2	8.865230397948666	6.666383111451378
3	3.3070212926418465	3.730168857130705
4	1.9671183287664347	2.958426079518334
5	1.4341738103019182	2.6961375053757344
6	1.214208501973403	2.7391433796946107
7	1.0633751476909927	2.7986899749053635
8	0.9364237411699641	2.8166484718736857
9	0.7566806606500918	2.5605035940623537
>10	6.136403630056059	36.93826530371126
>50	0.06913195404610473	1.7599327028955714
>100	0.026395836999421805	2.105397473523032
>500	0.002513889238040172	0.5434808293044863
>1k	0.00628472309510043	3.783099163984707
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCG	1918	0.9064315048747866	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGC	1842	0.8705145109381425	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTC	1638	0.7741057377397813	No Hit
GCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	1423	0.6724984522611165	TruSeq Adapter, Index 27 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1184	0.5595489581708798	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCACTTTGT	643	0.3038766723850302	No Hit
CCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	507	0.23960415691945616	TruSeq Adapter, Index 27 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGCT	436	0.20605012311022264	TruSeq Adapter, Index 20 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCT	402	0.18998199424382914	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTA	373	0.17627682550484644	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTC	304	0.14366797574657725	No Hit
GGGGAGGGGGAAATACTGATTTATCAATGATTTTATGAAGAAAGATAATAT	301	0.14225019967013078	No Hit
TCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	280	0.13232576713500538	TruSeq Adapter, Index 27 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	269	0.12712725485470158	No Hit
ACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC	232	0.10964134991186159	TruSeq Adapter, Index 20 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCT	232	0.10964134991186159	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCC	226	0.10680579775896862	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20983085931407994	0.0
2	0.0	0.0	0.0	1.4064338678349142	0.0
3	0.0	0.0	0.0	1.8119178256986093	0.0
4	0.0	0.0	0.0	2.3842267685575074	0.0
5	0.0	0.0	0.0	4.269868950231333	0.0
6	0.0	0.0	0.0	4.901251896275502	0.0
7	0.0	0.0	0.0	5.700405011365838	0.0
8	0.0	0.0	0.0	6.868652498357743	0.0
9	0.0	0.0	0.0	7.284533480782045	0.0
10	0.0	0.0	0.0	9.128587564213442	0.0
11	0.0	0.0	0.0	10.470748916582782	0.0
12	0.0	0.0	0.0	12.409793997136092	0.0
13	0.0	0.0	0.0	12.899399335535612	0.0
14	0.0	0.0	0.0	13.101668722441978	0.0
15	0.0	0.0	0.0	13.66594360086768	0.0
16	0.0	0.0	0.0	14.506684814200446	0.0
17	0.0	0.0	0.0	15.453286641241215	0.0
18	0.0	0.0	0.0	16.45565432728888	0.0
19	0.0	0.0	0.0	17.319552549870274	0.0
20	0.0	0.0	0.0	17.877683731964705	0.0
21	0.0	0.0	0.0	18.52371703079882	0.0
22	0.0	0.0	0.0	19.154154792792028	0.0
23	0.0	0.0	0.0	19.761908137562088	0.0
24	0.0	0.0	0.0	20.258129764318358	0.0
25	0.0	0.0	0.0	20.688661099532606	0.0
26	0.0	0.0	0.0	21.10690504208432	0.0
27	0.0	0.0	0.0	21.58091484364293	0.0
28	0.0	0.0	0.0	21.98356324935373	0.0
29	0.0	0.0	0.0	22.451901946606554	0.0
30	0.0	0.0	0.0	23.010033128700986	0.0
31	0.0	0.0	0.0	23.488296258488933	0.0
32	0.0	0.0	0.0	23.929697210289273	0.0
33	4.7259202548216204E-4	0.0	0.0	24.369680386013165	0.0
34	4.7259202548216204E-4	0.0	0.0	24.785088776411985	0.0
35	4.7259202548216204E-4	0.0	0.0	25.272331154684096	0.0
36	9.451840509643241E-4	0.0	0.0	25.693410649388703	0.0
37	9.451840509643241E-4	0.0	0.0	26.102675343456255	0.0
38	9.451840509643241E-4	0.0	0.0	26.561562200199432	0.0
39	9.451840509643241E-4	0.0	0.0	27.097008965070724	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTA	30	2.1570795E-6	45.000004	5
CGTAAGG	30	2.1570795E-6	45.000004	2
ACACGGG	25	3.8799844E-5	45.0	3
CGAACGG	25	3.8799844E-5	45.0	2
CACGGGC	20	7.0202263E-4	45.0	4
ATAGCTA	20	7.0202263E-4	45.0	27
GTAGGGA	90	0.0	45.0	4
TATGCGG	20	7.0202263E-4	45.0	2
GTCAGCG	20	7.0202263E-4	45.0	35
ACGGCTA	20	7.0202263E-4	45.0	30
CCTCCGC	20	7.0202263E-4	45.0	45
TACTGGC	20	7.0202263E-4	45.0	13
TCTCGTA	25	3.8799844E-5	45.0	28
TATTACG	20	7.0202263E-4	45.0	1
CGCAGTA	20	7.0202263E-4	45.0	37
GACGGGT	20	7.0202263E-4	45.0	4
CATACGG	20	7.0202263E-4	45.0	2
CGTACGG	25	3.8799844E-5	45.0	31
GCTAGCA	20	7.0202263E-4	45.0	30
TCGTACG	25	3.8799844E-5	45.0	30
>>END_MODULE