##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554386_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	354400
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08798250564334	33.0	31.0	34.0	30.0	34.0
2	32.164723476297965	34.0	31.0	34.0	30.0	34.0
3	32.21133182844244	34.0	31.0	34.0	30.0	34.0
4	35.78176918735892	37.0	35.0	37.0	35.0	37.0
5	35.85023984198646	37.0	35.0	37.0	35.0	37.0
6	35.864173250564335	37.0	35.0	37.0	35.0	37.0
7	35.98119638826185	37.0	35.0	37.0	35.0	37.0
8	35.93416760722348	37.0	35.0	37.0	35.0	37.0
9	37.73778781038375	39.0	38.0	39.0	35.0	39.0
10	37.45022573363431	39.0	37.0	39.0	35.0	39.0
11	37.41079571106095	39.0	37.0	39.0	35.0	39.0
12	37.33316591422122	39.0	37.0	39.0	35.0	39.0
13	37.29643058690745	39.0	37.0	39.0	34.0	39.0
14	38.54204853273138	40.0	38.0	41.0	34.0	41.0
15	38.62380925507901	40.0	38.0	41.0	35.0	41.0
16	38.662615688487584	40.0	38.0	41.0	35.0	41.0
17	38.57215293453724	40.0	38.0	41.0	34.0	41.0
18	38.562999435665915	40.0	38.0	41.0	35.0	41.0
19	38.50154345372461	40.0	37.0	41.0	34.0	41.0
20	38.368716139954856	40.0	37.0	41.0	34.0	41.0
21	38.349477990970655	40.0	37.0	41.0	34.0	41.0
22	38.33519751693002	40.0	37.0	41.0	34.0	41.0
23	38.27189898419864	40.0	37.0	41.0	34.0	41.0
24	38.20134593679458	40.0	37.0	41.0	34.0	41.0
25	38.11512133182844	40.0	37.0	41.0	34.0	41.0
26	38.099994356659145	40.0	37.0	41.0	34.0	41.0
27	38.03387133182844	40.0	36.0	41.0	34.0	41.0
28	37.9397262979684	40.0	36.0	41.0	34.0	41.0
29	37.947327878103835	40.0	36.0	41.0	34.0	41.0
30	37.80948645598194	40.0	36.0	41.0	33.0	41.0
31	37.771599887133185	40.0	36.0	41.0	33.0	41.0
32	37.62756489841986	40.0	36.0	41.0	33.0	41.0
33	37.559889954853276	40.0	36.0	41.0	33.0	41.0
34	37.422409706546276	40.0	36.0	41.0	33.0	41.0
35	37.34206546275395	40.0	36.0	41.0	33.0	41.0
36	37.241080699774265	40.0	35.0	41.0	33.0	41.0
37	37.17619356659142	40.0	35.0	41.0	33.0	41.0
38	37.08153781038375	40.0	35.0	41.0	32.0	41.0
39	37.01524830699774	40.0	35.0	41.0	32.0	41.0
40	36.953354966139955	40.0	35.0	41.0	32.0	41.0
41	36.883236455981944	40.0	35.0	41.0	31.0	41.0
42	36.84692720090293	40.0	35.0	41.0	31.0	41.0
43	36.79943284424379	39.0	35.0	41.0	31.0	41.0
44	36.740953724604964	39.0	35.0	41.0	31.0	41.0
45	36.68687641083521	39.0	35.0	41.0	31.0	41.0
46	36.583713318284424	39.0	35.0	41.0	31.0	41.0
47	36.45920711060948	39.0	35.0	41.0	31.0	41.0
48	36.43022009029345	39.0	35.0	41.0	31.0	41.0
49	36.36540914221219	39.0	35.0	41.0	31.0	41.0
50	36.304475169300225	39.0	35.0	41.0	31.0	41.0
51	35.516927200902934	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	9.0
10	5.0
11	7.0
12	7.0
13	11.0
14	12.0
15	21.0
16	44.0
17	50.0
18	80.0
19	144.0
20	305.0
21	563.0
22	1033.0
23	1426.0
24	1813.0
25	2236.0
26	2943.0
27	3290.0
28	3572.0
29	3753.0
30	4408.0
31	5625.0
32	7115.0
33	9821.0
34	18807.0
35	27359.0
36	23826.0
37	34877.0
38	62110.0
39	138926.0
40	202.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.30389390519187	18.658860045146728	22.622460496613993	12.414785553047404
2	34.331264108352144	25.594243792325056	22.920711060948083	17.153781038374717
3	32.568284424379236	25.172968397291196	25.952031602708804	16.306715575620768
4	28.679176072234764	24.759593679458238	27.127257336343114	19.43397291196388
5	27.699492099322796	28.418171557562076	23.908013544018058	19.974322799097067
6	25.806997742663658	35.79937923250564	23.864841986455982	14.528781038374719
7	77.26693002257336	6.552200902934537	11.580699774266366	4.600169300225733
8	77.48814898419865	7.730530474040632	9.420428893905191	5.360891647855531
9	71.58747178329571	7.94469525959368	12.042042889390519	8.42579006772009
10	37.41337471783296	27.844243792325056	19.37584650112867	15.366534988713317
11	29.91083521444695	25.960214446952595	25.8244920993228	18.30445823927765
12	26.518058690744923	23.36230248306998	29.068566591422123	21.05107223476298
13	25.032449209932278	25.298250564334086	30.334367945823924	19.334932279909705
14	21.340575620767492	28.584650112866818	28.864277652370202	21.210496613995485
15	20.198081264108353	25.284424379232505	32.89588036117381	21.62161399548533
16	22.85186230248307	24.992945823927766	30.443566591422123	21.711625282167045
17	22.56518058690745	24.577313769751694	29.37161399548533	23.48589164785553
18	23.964446952595935	23.55502257336343	29.683690744920995	22.79683972911964
19	24.432562076749438	25.497460496613993	28.422686230248306	21.647291196388263
20	26.721501128668173	24.939334085778782	28.67183972911964	19.66732505643341
21	25.062923250564335	26.92127539503386	28.217832957110613	19.797968397291196
22	22.66196388261851	23.465575620767495	29.40349887133183	24.46896162528217
23	22.486738148984198	26.631207674943568	28.443002257336342	22.439051918735892
24	22.18453724604966	25.494920993227993	30.053893905191874	22.266647855530476
25	22.105530474040634	27.646726862302483	27.812641083521445	22.43510158013544
26	20.909424379232505	26.314051918735892	30.161963882618508	22.614559819413095
27	23.49520316027088	25.946388261851016	28.089446952595935	22.468961625282166
28	20.381489841986454	27.823081264108353	29.635440180586908	22.159988713318285
29	22.418453724604966	25.61597065462754	29.511851015801355	22.45372460496614
30	22.81941309255079	26.775959367945823	28.886004514672685	21.5186230248307
31	24.794018058690746	26.658295711060948	25.771726862302486	22.775959367945823
32	25.554176072234764	26.315462753950335	27.009593679458238	21.12076749435666
33	23.456828442437924	27.183972911963885	26.020033860045146	23.33916478555305
34	22.855530474040634	26.163656884875845	28.47686230248307	22.50395033860045
35	23.40265237020316	26.40321670428894	27.927483069977427	22.266647855530476
36	22.75931151241535	29.979966139954854	26.098194130925506	21.16252821670429
37	23.77285553047404	28.097347629796836	26.93312641083521	21.196670428893903
38	23.494356659142213	27.685383747178328	25.129796839729117	23.69046275395034
39	24.16732505643341	26.15660270880361	26.08718961625282	23.58888261851016
40	23.151805869074494	24.74153498871332	28.998024830699777	23.108634311512414
41	21.07816027088036	26.976015801354404	27.625564334085777	24.32025959367946
42	23.705417607223474	26.370203160270883	27.20711060948081	22.71726862302483
43	23.66168171557562	26.243510158013546	27.35158013544018	22.743227990970656
44	22.891083521444695	25.753103837471787	27.522009029345373	23.83380361173815
45	23.369074492099323	24.288092550790065	26.99915349887133	25.343679458239276
46	23.775959367945823	26.09706546275395	26.91309255079007	23.21388261851016
47	21.489277652370202	27.36032731376975	29.335214446952595	21.81518058690745
48	22.279627539503384	26.48109480812641	28.1489841986456	23.090293453724605
49	23.040632054176072	24.719808126410836	29.58860045146727	22.650959367945823
50	21.752539503386004	25.11597065462754	28.51044018058691	24.62104966139955
51	21.88431151241535	24.638261851015802	27.19300225733634	26.284424379232508
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	288.0
1	545.5
2	803.0
3	1758.0
4	2713.0
5	1879.5
6	1046.0
7	1004.5
8	963.0
9	988.5
10	1014.0
11	1010.0
12	1006.0
13	989.5
14	973.0
15	991.5
16	1010.0
17	952.5
18	895.0
19	1045.0
20	1195.0
21	1382.5
22	1570.0
23	1771.5
24	1973.0
25	2096.0
26	2704.0
27	3189.0
28	3720.0
29	4251.0
30	4737.5
31	5224.0
32	5970.5
33	6717.0
34	7734.0
35	8751.0
36	9362.0
37	9973.0
38	11152.5
39	12332.0
40	13467.5
41	14603.0
42	15898.0
43	17193.0
44	19449.0
45	21705.0
46	25771.5
47	29838.0
48	31477.5
49	33117.0
50	33377.0
51	33637.0
52	30822.5
53	28008.0
54	25161.0
55	22314.0
56	20405.5
57	18497.0
58	17093.5
59	15690.0
60	14822.0
61	13954.0
62	12309.0
63	10664.0
64	9761.0
65	8858.0
66	7264.5
67	5671.0
68	4819.5
69	3968.0
70	3390.5
71	2813.0
72	2449.5
73	2086.0
74	1943.5
75	1358.0
76	915.0
77	737.0
78	559.0
79	401.0
80	243.0
81	160.0
82	77.0
83	63.5
84	50.0
85	36.0
86	22.0
87	12.5
88	3.0
89	2.0
90	1.0
91	1.5
92	2.0
93	3.0
94	4.0
95	2.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	354400.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.65605753847699
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.36743062328985	24.356024089691854
2	8.930871335745492	6.011558390891705
3	3.336221797042434	3.3685221828714385
4	2.096473224883364	2.8223609393820364
5	1.4748587504854587	2.481896548373244
6	1.2756577482166844	2.5760166344030844
7	1.0593286474608803	2.495697813777028
8	0.9478474579779856	2.5520646866725
9	0.8548193044944247	2.589286292735872
>10	7.52659223927873	44.29169741403284
>50	0.0821296088401383	1.8454991117283301
>100	0.04106480442006915	2.300457092351561
>500	0.002514171699187907	0.5971147210039546
>1k	0.004190286165313179	1.711804082084554
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1405	0.3964446952595937	No Hit
GCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC	1325	0.37387133182844245	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGC	1170	0.33013544018058694	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTC	1104	0.31151241534988716	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCG	1065	0.3005079006772009	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	766	0.21613995485327314	No Hit
CTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGCT	708	0.19977426636568849	Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTAGGCAGATCGTATGCCGTCTTCTGC	643	0.18143340857787812	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGGCAGAT	463	0.1306433408577878	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20682844243792325	0.0
2	0.0	0.0	0.0	0.9446952595936795	0.0
3	0.0	0.0	0.0	1.2107787810383748	0.0
4	0.0	0.0	0.0	1.588882618510158	0.0
5	0.0	0.0	0.0	2.724887133182844	0.0
6	0.0	0.0	0.0	3.2483069977426635	0.0
7	0.0	0.0	0.0	3.80558690744921	0.0
8	0.0	0.0	0.0	4.7508465011286685	0.0
9	0.0	0.0	0.0	5.095936794582393	0.0
10	0.0	0.0	0.0	6.167889390519187	0.0
11	0.0	0.0	0.0	7.385440180586907	0.0
12	0.0	0.0	0.0	8.633747178329571	0.0
13	0.0	0.0	0.0	9.058690744920993	0.0
14	0.0	0.0	0.0	9.222911963882618	0.0
15	0.0	0.0	0.0	9.620203160270881	0.0
16	0.0	0.0	0.0	10.308408577878104	0.0
17	0.0	0.0	0.0	11.031038374717832	0.0
18	0.0	0.0	0.0	11.872460496613995	0.0
19	0.0	0.0	0.0	12.455135440180587	0.0
20	0.0	0.0	0.0	12.914785553047404	0.0
21	0.0	0.0	0.0	13.443002257336342	0.0
22	0.0	0.0	0.0	13.999435665914222	0.0
23	0.0	0.0	0.0	14.575902934537247	0.0
24	0.0	0.0	0.0	15.04176072234763	0.0
25	0.0	0.0	0.0	15.442155756207676	0.0
26	0.0	0.0	0.0	15.818284424379232	0.0
27	0.0	0.0	0.0	16.19920993227991	0.0
28	0.0	0.0	0.0	16.591139954853272	0.0
29	2.821670428893905E-4	0.0	0.0	16.996613995485326	0.0
30	2.821670428893905E-4	0.0	0.0	17.484480812641085	0.0
31	2.821670428893905E-4	0.0	0.0	17.90208803611738	0.0
32	2.821670428893905E-4	0.0	0.0	18.31010158013544	0.0
33	2.821670428893905E-4	0.0	0.0	18.736738148984198	0.0
34	2.821670428893905E-4	0.0	0.0	19.12443566591422	0.0
35	2.821670428893905E-4	0.0	0.0	19.572234762979683	0.0
36	2.821670428893905E-4	0.0	0.0	19.973476297968396	0.0
37	2.821670428893905E-4	0.0	0.0	20.387133182844245	0.0
38	2.821670428893905E-4	0.0	0.0	20.812641083521445	0.0
39	2.821670428893905E-4	0.0	0.0	21.24181715575621	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGG	30	2.1612377E-6	45.000004	2
TCCATAA	20	7.0266775E-4	45.0	30
GCGACAG	35	1.2087548E-7	45.0	1
CCGATCG	35	1.2087548E-7	45.0	40
GTTAGCG	20	7.0266775E-4	45.0	1
ACGATAT	20	7.0266775E-4	45.0	32
TACGATA	20	7.0266775E-4	45.0	31
GCTTACG	20	7.0266775E-4	45.0	28
TTACGAT	20	7.0266775E-4	45.0	30
CGCACGG	20	7.0266775E-4	45.0	2
CGATCGA	25	3.885328E-5	45.0	41
CGATCAT	20	7.0266775E-4	45.0	10
CGATATG	25	3.885328E-5	45.0	10
TCGAATG	45	3.8380676E-10	45.0	44
CCGTTGG	20	7.0266775E-4	45.0	2
CTTACGA	20	7.0266775E-4	45.0	29
TCGGCGT	140	0.0	41.785713	4
GCTACGA	65	0.0	41.53846	10
AGCTACG	65	0.0	41.53846	9
GGCACCG	60	3.6379788E-12	41.250004	8
>>END_MODULE