FastQCFastQC Report
Sat 18 Jun 2016
SRR3554385_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554385_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241413
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21590.8943180358969898No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGC18650.7725350333246345No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTC18280.7572086010281137No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCG16750.6938317323425003No Hit
GCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC15660.6486808912527495No Hit
CCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC8860.36700591931668963No Hit
CTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGCT7000.28995952993417917No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG6740.27918960453662395No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTGCTAGT5870.24315177724480455No Hit
TCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC4660.19303020135618215No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCC4070.1685907552617299No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCT4000.1656911599623881No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTA3910.16196310886323437No Hit
GAACTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCT3230.1337956116696284No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTC2970.12302568627207318No Hit
ACTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC2810.11639803987357764No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCT207.0222036E-445.0000049
ACACCAC207.0222036E-445.00000425
ATAGCGG207.0222036E-445.0000042
CGCGCCT207.0222036E-445.00000417
CCGTCGA207.0222036E-445.00000441
TCAATTA207.0222036E-445.00000422
GCCGATC207.0222036E-445.0000049
CCCGTCG207.0222036E-445.00000440
ATGACGG207.0222036E-445.0000042
CGTCGAA207.0222036E-445.00000442
CGCACGG207.0222036E-445.0000042
GCGTAAG207.0222036E-445.0000041
TGCGTAG207.0222036E-445.0000041
CTGCGCT253.8816237E-545.013
CATACGA253.8816237E-545.018
CCGACGG253.8816237E-545.02
ATACGAA253.8816237E-545.019
TCGCGAG253.8816237E-545.01
GCGATAT302.1583564E-644.9999969
GCATACC302.1583564E-644.99999613