##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554385_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	241413
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.934870947297785	33.0	31.0	34.0	30.0	34.0
2	32.030516169386075	33.0	31.0	34.0	30.0	34.0
3	32.05748240566995	33.0	31.0	34.0	30.0	34.0
4	35.67764784829318	37.0	35.0	37.0	33.0	37.0
5	35.75824002849888	37.0	35.0	37.0	35.0	37.0
6	35.77070000372805	37.0	35.0	37.0	35.0	37.0
7	35.93823447784502	37.0	35.0	37.0	35.0	37.0
8	35.877148289445884	37.0	35.0	37.0	35.0	37.0
9	37.71698292966825	39.0	38.0	39.0	35.0	39.0
10	37.34423166937986	39.0	37.0	39.0	34.0	39.0
11	37.31654053427115	39.0	37.0	39.0	34.0	39.0
12	37.21172430647894	39.0	37.0	39.0	34.0	39.0
13	37.130125552476464	39.0	37.0	39.0	33.0	39.0
14	38.28902751715939	40.0	38.0	41.0	33.0	41.0
15	38.382808713698104	40.0	38.0	41.0	34.0	41.0
16	38.467551457460864	40.0	38.0	41.0	34.0	41.0
17	38.39053406403135	40.0	37.0	41.0	34.0	41.0
18	38.345209247223636	40.0	37.0	41.0	34.0	41.0
19	38.23389378368191	40.0	37.0	41.0	34.0	41.0
20	38.022355879757924	40.0	36.0	41.0	34.0	41.0
21	38.029178213269375	40.0	36.0	41.0	34.0	41.0
22	38.038137962744344	40.0	36.0	41.0	34.0	41.0
23	37.920136860898126	40.0	35.0	41.0	34.0	41.0
24	37.882139735639754	40.0	35.0	41.0	34.0	41.0
25	37.81222635069363	40.0	35.0	41.0	34.0	41.0
26	37.789191137179856	40.0	35.0	41.0	34.0	41.0
27	37.68166585892226	40.0	35.0	41.0	33.0	41.0
28	37.588974081760306	40.0	35.0	41.0	33.0	41.0
29	37.59413536139313	40.0	35.0	41.0	33.0	41.0
30	37.41151056488259	40.0	35.0	41.0	33.0	41.0
31	37.35003914453654	40.0	35.0	41.0	33.0	41.0
32	37.09047151561846	40.0	35.0	41.0	32.0	41.0
33	36.92830957736327	40.0	35.0	41.0	31.0	41.0
34	36.70166892420872	40.0	35.0	41.0	31.0	41.0
35	36.53889392866167	40.0	35.0	41.0	30.0	41.0
36	36.40696234254162	40.0	35.0	41.0	30.0	41.0
37	36.29172414078778	39.0	35.0	41.0	30.0	41.0
38	36.261477219536644	39.0	35.0	41.0	30.0	41.0
39	36.1951634750407	39.0	35.0	41.0	30.0	41.0
40	36.14107359587098	39.0	35.0	41.0	29.0	41.0
41	36.098358414832674	39.0	35.0	41.0	29.0	41.0
42	36.020280597979394	39.0	35.0	41.0	29.0	41.0
43	35.93212047404241	39.0	35.0	41.0	29.0	41.0
44	35.89467841416991	39.0	35.0	41.0	28.0	41.0
45	35.834023022786674	39.0	35.0	41.0	27.0	41.0
46	35.76483867894438	39.0	35.0	41.0	27.0	41.0
47	35.621905199802825	39.0	35.0	41.0	27.0	41.0
48	35.620819922705074	39.0	35.0	41.0	27.0	41.0
49	35.56842837792497	39.0	35.0	41.0	27.0	41.0
50	35.4519764884244	38.0	35.0	41.0	27.0	41.0
51	34.684905121099526	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	5.0
11	5.0
12	6.0
13	8.0
14	12.0
15	11.0
16	23.0
17	45.0
18	76.0
19	153.0
20	357.0
21	621.0
22	875.0
23	1317.0
24	1766.0
25	2498.0
26	3238.0
27	3499.0
28	3438.0
29	3321.0
30	3718.0
31	4336.0
32	5256.0
33	7239.0
34	12953.0
35	19967.0
36	15611.0
37	22461.0
38	41290.0
39	87206.0
40	98.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.09554994967131	19.275266866324515	23.589450443845195	16.03973274015898
2	34.26658879182148	26.105470707874055	23.15616806054355	16.471772439760908
3	28.608649907005834	26.57810474166677	29.401896335325773	15.411349016001624
4	26.302643188229304	22.65453807375742	33.17592673136907	17.866892006644218
5	26.272404551536166	26.47123394349103	27.91647508626296	19.339886418709845
6	23.110188763653987	34.7881017178031	27.71888837800781	14.382821140535098
7	73.26821670746811	5.287204914399805	16.61426683732856	4.830311540803519
8	73.98731634170488	7.678956808456877	12.4193808949808	5.914345954857443
9	69.59940019800094	5.845998351372959	14.604018839084887	9.950582611541217
10	37.550173354376106	22.418428170811016	23.41713163748431	16.61426683732856
11	30.3753319001048	23.969711655958875	27.650126546623422	18.004829897312906
12	25.60591186058746	20.596653867024557	32.205804989789286	21.591629282598703
13	24.47714083334369	23.10977453575408	34.08888502276182	18.324199608140407
14	19.686180943031236	28.386623752656238	31.007029447461402	20.92016585685112
15	18.16679300617614	23.59939191344294	37.549759126476204	20.68405595390472
16	19.847729823994563	22.734898286339178	33.85898853831401	23.558383351352248
17	20.291782132693765	23.62300290373758	31.767966099588673	24.317248863979984
18	21.80329973945065	21.773061102757516	32.98993840431129	23.43370075348055
19	21.121480616205424	24.9497748671364	30.983004229266857	22.945740287391317
20	23.29493440701205	24.473412782244537	32.343328652558064	19.88832415818535
21	21.626838654090708	27.288919817905416	31.63168512051961	19.45255640748427
22	19.085964716067487	23.453583692676037	32.32178880176295	25.13866278949352
23	19.47616739777891	25.97167509620443	31.65736725031378	22.894790255702883
24	21.20018391718756	24.986226922328125	31.63872699481801	22.174862165666305
25	19.466640156081073	27.4194016063758	29.67114447026465	23.44281376727848
26	19.095077729865416	25.216123406775942	33.02141972470414	22.667379138654507
27	22.387775306217975	24.432404220153845	32.0057329141347	21.174087559493483
28	17.78321797086321	25.686272073169214	34.76656186700799	21.763948088959584
29	22.330611856030952	21.823596906546044	33.455530563805596	22.39026067361741
30	21.04484845472282	24.929891927940915	32.90667859643018	21.11858102090608
31	22.50665871349099	23.491692659467386	30.364561974707243	23.637086652334382
32	25.143633524292397	26.53585349587636	28.025831251838135	20.294681727993108
33	22.92171506919677	23.04391229966903	29.283841383852565	24.75053124728163
34	22.560094112578856	22.457779821302086	32.095620368414295	22.886505697704763
35	22.501273750792212	23.908820154672696	30.28751558532473	23.30239050921036
36	24.95723096933471	24.413763964658074	30.48593074937969	20.143074316627523
37	22.37700538082042	23.417545865384216	33.95923169009125	20.24621706370411
38	22.101129599483045	25.360688943843122	27.980680410748388	24.55750104592545
39	24.32801878937754	23.112259903153518	29.595754992481766	22.96396631498718
40	22.57832014017472	22.681462887251307	30.6458227187434	24.094394253830572
41	21.386172244245337	23.953142539962638	29.8550616578229	24.805623557969124
42	23.427901562881868	24.204164647305653	29.88654297821575	22.481390811596725
43	25.206181937178197	22.855438605211813	29.43669147891787	22.501687978692118
44	23.186406697236688	22.246109364450135	30.391072560301225	24.176411378011956
45	22.079175520788027	22.366235455422864	29.611909880578096	25.942679143211013
46	24.931548839540536	23.668567972727235	28.870856167646313	22.529027020085913
47	20.196095487815484	24.637861258507204	33.45925861490475	21.70678463877256
48	21.512925981616565	23.64330007083297	30.34343635181204	24.500337595738422
49	22.54352499658262	20.560201811832833	33.12373401598091	23.772539175603633
50	21.89277296583034	21.125208667304577	31.899276343858862	25.082742023006215
51	21.911827449226013	21.108225323408433	28.855529735349794	28.12441749201576
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	147.0
1	237.0
2	327.0
3	2005.0
4	3683.0
5	2455.0
6	1227.0
7	1267.0
8	1307.0
9	1266.5
10	1226.0
11	1218.0
12	1210.0
13	1183.0
14	1156.0
15	1126.5
16	1097.0
17	1044.5
18	992.0
19	920.0
20	848.0
21	844.0
22	840.0
23	907.0
24	974.0
25	979.0
26	1070.0
27	1156.0
28	1505.0
29	1854.0
30	2130.5
31	2407.0
32	2684.5
33	2962.0
34	3851.5
35	4741.0
36	5056.0
37	5371.0
38	6001.5
39	6632.0
40	7703.0
41	8774.0
42	10373.0
43	11972.0
44	13770.5
45	15569.0
46	22114.5
47	28660.0
48	28648.5
49	28637.0
50	28083.5
51	27530.0
52	24172.5
53	20815.0
54	18047.5
55	15280.0
56	13168.0
57	11056.0
58	10068.0
59	9080.0
60	7921.5
61	6763.0
62	6149.5
63	5536.0
64	4762.5
65	3989.0
66	3253.5
67	2518.0
68	1949.0
69	1380.0
70	1218.0
71	1056.0
72	917.5
73	779.0
74	580.5
75	295.0
76	208.0
77	204.0
78	200.0
79	136.0
80	72.0
81	42.0
82	12.0
83	7.0
84	2.0
85	1.0
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	241413.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.842978629982646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.59476176257304	28.974827370522714
2	9.654267798489954	7.500010355697498
3	3.558631574457194	4.146835505958668
4	1.9163502964637633	2.9774701445241143
5	1.3650130102802542	2.6510585593982094
6	1.102674572367018	2.5698698910166398
7	0.9256494475962974	2.5168487198286753
8	0.7731519003540502	2.4025218194546274
9	0.644115514225995	2.2517428638888544
>10	5.3651409802499685	35.28558942559017
>50	0.06398498485688692	1.6743091714199319
>100	0.02666041035703622	2.103035047822611
>500	0.004265665657125795	1.1793068310322974
>1k	0.005332082071407243	3.7665742938449878
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2159	0.8943180358969898	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGC	1865	0.7725350333246345	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTC	1828	0.7572086010281137	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCG	1675	0.6938317323425003	No Hit
GCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC	1566	0.6486808912527495	No Hit
CCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC	886	0.36700591931668963	No Hit
CTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGCT	700	0.28995952993417917	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	674	0.27918960453662395	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTGCTAGT	587	0.24315177724480455	No Hit
TCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC	466	0.19303020135618215	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCC	407	0.1685907552617299	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCT	400	0.1656911599623881	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTA	391	0.16196310886323437	No Hit
GAACTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCT	323	0.1337956116696284	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTC	297	0.12302568627207318	No Hit
ACTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC	281	0.11639803987357764	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2974156321324867	0.0
2	0.0	0.0	0.0	1.6660246134218124	0.0
3	0.0	0.0	0.0	2.20286397169995	0.0
4	0.0	0.0	0.0	2.912436364238877	0.0
5	0.0	0.0	0.0	5.034525895457163	0.0
6	0.0	0.0	0.0	5.916002866457068	0.0
7	0.0	0.0	0.0	6.88819574753638	0.0
8	0.0	0.0	0.0	8.486701213273518	0.0
9	0.0	0.0	0.0	9.080704021738681	0.0
10	4.1422789990597025E-4	0.0	0.0	11.129060986773704	0.0
11	4.1422789990597025E-4	0.0	0.0	13.016283298745304	0.0
12	4.1422789990597025E-4	0.0	0.0	15.181038303653905	0.0
13	4.1422789990597025E-4	0.0	0.0	15.851673273601671	0.0
14	4.1422789990597025E-4	0.0	0.0	16.09731041824591	0.0
15	4.1422789990597025E-4	0.0	0.0	16.68137175711333	0.0
16	4.1422789990597025E-4	0.0	0.0	17.61338453190176	0.0
17	4.1422789990597025E-4	0.0	0.0	18.676707550960387	0.0
18	4.1422789990597025E-4	0.0	0.0	20.02709050465385	0.0
19	4.1422789990597025E-4	0.0	0.0	20.903596740854884	0.0
20	4.1422789990597025E-4	0.0	0.0	21.544821529909324	0.0
21	4.1422789990597025E-4	0.0	0.0	22.27220572214421	0.0
22	4.1422789990597025E-4	0.0	0.0	22.993376495880504	0.0
23	4.1422789990597025E-4	0.0	0.0	23.704605800019053	0.0
24	4.1422789990597025E-4	0.0	0.0	24.28203949248798	0.0
25	4.1422789990597025E-4	0.0	0.0	24.797338999971004	0.0
26	4.1422789990597025E-4	0.0	0.0	25.298968986757135	0.0
27	4.1422789990597025E-4	0.0	0.0	25.84616404253292	0.0
28	4.1422789990597025E-4	0.0	0.0	26.363948917415385	0.0
29	4.1422789990597025E-4	0.0	0.0	26.887118754996624	0.0
30	4.1422789990597025E-4	0.0	0.0	27.441769912970717	0.0
31	4.1422789990597025E-4	0.0	0.0	27.968253573751205	0.0
32	4.1422789990597025E-4	0.0	0.0	28.45621403984044	0.0
33	4.1422789990597025E-4	0.0	0.0	28.95784402662657	0.0
34	4.1422789990597025E-4	0.0	0.0	29.459059785512792	0.0
35	4.1422789990597025E-4	0.0	0.0	29.997141827490648	0.0
36	4.1422789990597025E-4	0.0	0.0	30.459834391685618	0.0
37	4.1422789990597025E-4	0.0	0.0	30.96312129007137	0.0
38	4.1422789990597025E-4	0.0	0.0	31.4369980075638	0.0
39	4.1422789990597025E-4	0.0	0.0	31.96223898464457	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGCT	20	7.0222036E-4	45.000004	9
ACACCAC	20	7.0222036E-4	45.000004	25
ATAGCGG	20	7.0222036E-4	45.000004	2
CGCGCCT	20	7.0222036E-4	45.000004	17
CCGTCGA	20	7.0222036E-4	45.000004	41
TCAATTA	20	7.0222036E-4	45.000004	22
GCCGATC	20	7.0222036E-4	45.000004	9
CCCGTCG	20	7.0222036E-4	45.000004	40
ATGACGG	20	7.0222036E-4	45.000004	2
CGTCGAA	20	7.0222036E-4	45.000004	42
CGCACGG	20	7.0222036E-4	45.000004	2
GCGTAAG	20	7.0222036E-4	45.000004	1
TGCGTAG	20	7.0222036E-4	45.000004	1
CTGCGCT	25	3.8816237E-5	45.0	13
CATACGA	25	3.8816237E-5	45.0	18
CCGACGG	25	3.8816237E-5	45.0	2
ATACGAA	25	3.8816237E-5	45.0	19
TCGCGAG	25	3.8816237E-5	45.0	1
GCGATAT	30	2.1583564E-6	44.999996	9
GCATACC	30	2.1583564E-6	44.999996	13
>>END_MODULE