##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554379_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	154925
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.816369210908505	33.0	31.0	34.0	30.0	34.0
2	31.881736324027756	33.0	31.0	34.0	30.0	34.0
3	31.89987413264483	33.0	31.0	34.0	30.0	34.0
4	35.61194771663708	37.0	35.0	37.0	33.0	37.0
5	31.802659351299017	37.0	35.0	37.0	0.0	37.0
6	33.55208649346458	37.0	35.0	37.0	17.0	37.0
7	35.25860254962078	37.0	35.0	37.0	32.0	37.0
8	35.19266741971922	37.0	35.0	37.0	32.0	37.0
9	37.093922865902854	39.0	37.0	39.0	33.0	39.0
10	36.932702920768115	39.0	37.0	39.0	33.0	39.0
11	37.064992738421815	39.0	37.0	39.0	34.0	39.0
12	36.9742778763918	39.0	37.0	39.0	33.0	39.0
13	36.908194287558494	39.0	37.0	39.0	33.0	39.0
14	38.01907374536066	40.0	37.0	41.0	33.0	41.0
15	38.13456833951912	40.0	37.0	41.0	33.0	41.0
16	38.065405841536226	40.0	37.0	41.0	33.0	41.0
17	37.935969017266416	40.0	37.0	41.0	33.0	41.0
18	37.96169759561078	39.0	37.0	41.0	33.0	41.0
19	37.73967403582379	39.0	37.0	41.0	33.0	41.0
20	37.455659189930614	39.0	35.0	41.0	32.0	41.0
21	37.524143940616426	39.0	35.0	41.0	33.0	41.0
22	37.5940358237857	39.0	35.0	41.0	33.0	41.0
23	37.3628013554946	39.0	35.0	41.0	33.0	41.0
24	37.410682588349204	39.0	35.0	41.0	33.0	41.0
25	37.31606906567694	39.0	35.0	41.0	33.0	41.0
26	37.279870905276745	39.0	35.0	41.0	33.0	41.0
27	37.15502985315475	39.0	35.0	41.0	32.0	41.0
28	37.00134258512183	39.0	35.0	41.0	32.0	41.0
29	36.955249314184286	39.0	35.0	41.0	32.0	41.0
30	36.76089720832661	39.0	35.0	41.0	31.0	41.0
31	36.66692270453445	39.0	35.0	41.0	31.0	41.0
32	36.23326125544618	39.0	35.0	41.0	30.0	41.0
33	35.94721962239793	39.0	35.0	41.0	27.0	41.0
34	35.57398095852832	39.0	35.0	41.0	25.0	41.0
35	35.450973051476524	39.0	35.0	41.0	24.0	41.0
36	35.20180732612555	39.0	35.0	41.0	23.0	41.0
37	35.047171211876716	39.0	35.0	41.0	21.0	41.0
38	34.91166048087784	39.0	35.0	41.0	21.0	41.0
39	34.91293851863805	39.0	35.0	41.0	21.0	41.0
40	34.8337776343392	38.0	35.0	41.0	21.0	41.0
41	34.77192189769243	38.0	34.0	41.0	21.0	41.0
42	34.646345005647895	38.0	35.0	41.0	20.0	41.0
43	34.57055994836211	38.0	35.0	41.0	18.0	41.0
44	34.5864127803776	38.0	35.0	40.0	19.0	41.0
45	34.52920445376795	38.0	34.0	40.0	20.0	41.0
46	34.37522026787155	38.0	34.0	40.0	20.0	41.0
47	34.210553493625945	37.0	34.0	40.0	20.0	41.0
48	34.224192351137646	37.0	34.0	40.0	20.0	41.0
49	34.18866548329837	37.0	34.0	40.0	20.0	41.0
50	34.083440374374696	37.0	34.0	40.0	18.0	41.0
51	33.27003388736485	36.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	2.0
11	0.0
12	5.0
13	2.0
14	6.0
15	11.0
16	22.0
17	28.0
18	53.0
19	194.0
20	405.0
21	857.0
22	1335.0
23	1602.0
24	1867.0
25	2407.0
26	2791.0
27	2999.0
28	2793.0
29	2644.0
30	2813.0
31	3285.0
32	4252.0
33	5930.0
34	9310.0
35	12785.0
36	12511.0
37	17567.0
38	27308.0
39	39113.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.7973212844925	14.97886073906729	19.221558818783283	18.00225915765693
2	35.088591253832504	27.694045505889946	21.17540745522027	16.041955785057286
3	28.276262707761816	28.270453445215427	29.19283524285945	14.260448604163306
4	24.74939486848475	22.231402291431337	36.27368081329676	16.745522026787153
5	25.538163627561723	33.26835565596256	25.216072293045023	15.977408423430692
6	21.420687429401323	35.89930611586251	28.83976117476198	13.84024527997418
7	68.39632080038729	5.3006293367758595	22.341778279812814	3.9612715830240437
8	67.65467161529773	11.596578989833791	15.289333548491205	5.459415846377279
9	62.54381152170405	6.865902856220751	17.405841536227207	13.184444085847991
10	37.21671776666129	25.554945941584638	23.610133935775377	13.618202355978697
11	32.85525254155237	22.468291108600937	28.853961594319834	15.822494755526867
12	24.48087784411812	20.132967564950782	32.81329675649508	22.572857834436018
13	26.820074229465867	22.24043892205906	35.04211715346135	15.897369695013715
14	19.333871228013557	29.66984024527997	32.09036630627723	18.90592222042924
15	17.469743424237535	21.56204615136356	38.906567694045506	22.061642730353398
16	20.927545586574148	22.355333225754396	32.47765047603679	24.239470711634663
17	20.0871389381959	25.41035985154107	33.32903017589156	21.17347103437147
18	23.515894787800548	19.282878812328548	33.18767145392932	24.013554945941586
19	21.466516056156205	23.205421978376634	31.33645312247862	23.991608842988544
20	26.738744553816364	22.014523156365982	33.436178796191705	17.810553493625946
21	22.126835565596256	28.851379699854768	31.573987413264483	17.44779732128449
22	20.351137647248667	21.190898822010652	35.3480716475714	23.109891883169276
23	22.289494916895272	25.480071002097787	31.77343876069066	20.45699532031628
24	20.216879135065355	25.04050346942069	32.48410521219945	22.258512183314508
25	18.632241407132483	28.946909795062126	31.240277553654995	21.180571244150396
26	19.826367597224465	21.4400516378893	36.45312247861869	22.28045828626755
27	24.748749394868486	22.662578667096984	31.150556720994025	21.438115217040504
28	17.442633532354364	27.18283040180733	34.557366467645636	20.817169598192674
29	22.039696627400353	23.28997902210747	34.12425367113119	20.54607067936098
30	22.445054058415362	26.488300790705182	32.147167984508634	18.919477166370825
31	23.805712441503953	22.193319348071647	30.086816201387766	23.91415200903663
32	26.22946587058254	25.827981281265128	29.103114410198483	18.83943843795385
33	24.98757463288688	24.080684202033243	27.837986122317247	23.093755042762627
34	23.948362110698724	23.491366790382443	30.9104405357431	21.649830563175733
35	24.64482814264967	25.700177505244476	28.66161045667258	20.993383895433272
36	23.95546232047765	29.291592706148133	27.008552525415524	19.74439244795869
37	23.84444085847991	26.183637243827658	29.20961755688236	20.76230434081007
38	20.909472325318703	29.31418428271744	26.992415685008876	22.78392770695498
39	24.316927545586577	27.497176052928836	26.44763595288043	21.738260448604162
40	23.888333064385993	26.398579958044216	28.216879135065355	21.49620784250444
41	22.38954332741649	24.48281426496692	26.11328061965467	27.014361787961917
42	23.73019202840084	24.537034048733258	28.945618847829596	22.78715507503631
43	23.512667419719218	23.597224463450058	28.115539777311604	24.774568339519124
44	23.09310956914636	23.30482491528159	30.47216395029853	23.12990156527352
45	21.468452477005002	23.233822817492335	29.60077456833952	25.696950137163142
46	24.840406648378245	26.443117637566566	27.701145715668872	21.015329998386317
47	19.985799580442148	26.868484750685816	32.087784411812166	21.057931257059867
48	22.010650314668386	25.70405034694207	29.15927061481362	23.126028723575924
49	22.04227852186542	22.468936582217204	32.90301758915604	22.585767306761337
50	21.767952234952396	23.5584960464741	30.837502017105052	23.836049701468454
51	21.591737937711798	22.10553493625948	28.866225593028883	27.43650153299984
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	49.0
1	196.5
2	344.0
3	2259.0
4	4174.0
5	2698.0
6	1222.0
7	1129.0
8	1036.0
9	1033.5
10	1031.0
11	973.0
12	915.0
13	957.0
14	999.0
15	957.5
16	916.0
17	910.0
18	904.0
19	791.5
20	679.0
21	740.0
22	801.0
23	821.0
24	841.0
25	859.5
26	1074.5
27	1271.0
28	1429.5
29	1588.0
30	1746.0
31	1904.0
32	2178.5
33	2453.0
34	2597.0
35	2741.0
36	3102.0
37	3463.0
38	3821.0
39	4179.0
40	4780.5
41	5382.0
42	5810.0
43	6238.0
44	7722.0
45	9206.0
46	12874.5
47	16543.0
48	15578.5
49	14614.0
50	14807.5
51	15001.0
52	13736.0
53	12471.0
54	11304.0
55	10137.0
56	9004.0
57	7871.0
58	6954.0
59	6037.0
60	5647.0
61	5257.0
62	4669.0
63	4081.0
64	3588.0
65	3095.0
66	2670.0
67	2245.0
68	1751.5
69	1258.0
70	1055.5
71	853.0
72	838.5
73	824.0
74	741.5
75	528.0
76	397.0
77	284.5
78	172.0
79	142.5
80	113.0
81	91.0
82	69.0
83	39.5
84	10.0
85	6.0
86	2.0
87	1.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	154925.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.93367758592867
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.4564308504786	35.88381474907213
2	8.645065463747386	8.114894303695335
3	3.674771702057432	5.174116507987736
4	2.302233469028496	4.322091334516702
5	1.691605237099791	3.969662740035501
6	1.3010232148751237	3.6637082459254477
7	1.0658488282539333	3.5016943682426978
8	0.9104411926504565	3.418428271744393
9	0.7454065353724282	3.1486203001452315
>10	3.1521619540103423	21.15797966758109
>50	0.02750577621300473	0.8842988542843312
>100	0.01925404334910331	1.8053897046958205
>500	0.002750577621300473	0.8868807487493949
>1k	0.005501155242600946	4.068420203324189
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2282	1.472970792318864	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCG	1358	0.8765531708891399	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGC	1345	0.8681620138776828	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTC	1318	0.8507342262385025	No Hit
GCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	794	0.5125060513151525	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTAAGACT	580	0.37437469743424234	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTA	300	0.19364208487978055	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	296	0.1910601904147168	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCT	278	0.17944166532192996	No Hit
CCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	260	0.16782314022914313	TruSeq Adapter, Index 14 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCT	254	0.16395029853154752	No Hit
CTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGCT	228	0.14716798450863322	TruSeq Adapter, Index 15 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTC	219	0.1413587219622398	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCG	167	0.10779409391641116	No Hit
GAATATGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTC	159	0.10263030498628369	No Hit
TCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	157	0.1013393577537518	TruSeq Adapter, Index 14 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1542681942875585	0.0
2	0.0	0.0	0.0	1.0521219945134743	0.0
3	0.0	0.0	0.0	1.3090204937873164	0.0
4	0.0	0.0	0.0	1.7453606583830885	0.0
5	0.0	0.0	0.0	3.333871228013555	0.0
6	0.0	0.0	0.0	4.027109891883169	0.0
7	0.0	0.0	0.0	4.827497176052929	0.0
8	0.0	0.0	0.0	6.159109246409553	0.0
9	0.0	0.0	0.0	6.725189607874778	0.0
10	0.0	0.0	0.0	8.804905599483622	0.0
11	0.0	0.0	0.0	9.997095368726804	0.0
12	0.0	0.0	0.0	12.092948200742295	0.0
13	0.0	0.0	0.0	12.656446667742456	0.0
14	0.0	0.0	0.0	12.913990640632564	0.0
15	0.0	0.0	0.0	13.486525738260449	0.0
16	0.0	0.0	0.0	14.249475552686784	0.0
17	0.0	0.0	0.0	15.100209778925286	0.0
18	0.0	0.0	0.0	16.091657253509762	0.0
19	0.0	0.0	0.0	16.98628368565435	0.0
20	0.0	0.0	0.0	17.518799419073744	0.0
21	0.0	0.0	0.0	18.001613684040663	0.0
22	0.0	0.0	0.0	18.525738260448605	0.0
23	0.0	0.0	0.0	19.024043892205906	0.0
24	0.0	0.0	0.0	19.421655639825723	0.0
25	0.0	0.0	0.0	19.790866548329838	0.0
26	0.0	0.0	0.0	20.13813135388091	0.0
27	0.0	0.0	0.0	20.549943521058577	0.0
28	0.0	0.0	0.0	20.87526222365661	0.0
29	0.0	0.0	0.0	21.197999031789575	0.0
30	0.0	0.0	0.0	21.63692109085041	0.0
31	0.0	0.0	0.0	21.993868000645474	0.0
32	0.0	0.0	0.0	22.338550911731485	0.0
33	0.0	0.0	0.0	22.663869614329514	0.0
34	0.0	0.0	0.0	23.00403421010166	0.0
35	0.0	0.0	0.0	23.360981119896724	0.0
36	0.0	0.0	0.0	23.709536872680328	0.0
37	0.0	0.0	0.0	24.049055994836213	0.0
38	0.0	0.0	0.0	24.384702275294497	0.0
39	0.0	0.0	0.0	24.810714862030014	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTAGG	30	2.1533142E-6	45.000004	2
CGGCGAG	70	0.0	45.000004	31
CAAACGA	30	2.1533142E-6	45.000004	15
ACGATGA	30	2.1533142E-6	45.000004	18
GCGATCC	30	2.1533142E-6	45.000004	33
TAATACG	50	2.1827873E-11	45.0	4
ACACGAA	25	3.8751412E-5	45.0	45
CGCGGAT	25	3.8751412E-5	45.0	23
GGCGTAA	20	7.014378E-4	45.0	7
CGCGATA	20	7.014378E-4	45.0	43
TCGATCA	20	7.014378E-4	45.0	17
GTAATAG	20	7.014378E-4	45.0	1
AATGCGG	20	7.014378E-4	45.0	2
TGACCTT	20	7.014378E-4	45.0	35
CACTCGA	40	6.7502697E-9	45.0	41
GCCGACT	40	6.7502697E-9	45.0	43
CGCTCGA	20	7.014378E-4	45.0	41
CCGAGAA	20	7.014378E-4	45.0	18
CGAATGC	100	0.0	45.0	45
CGGGAGT	45	3.8016879E-10	45.0	6
>>END_MODULE