SRR3554378_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554378_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	358838
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTC	2275	0.6339908259437407	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2140	0.5963693923163099	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGC	1992	0.5551251539692006	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCG	1988	0.5540104448246841	No Hit
GCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	1262	0.35169073509494536	Illumina PCR Primer Index 7 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCT	536	0.14937102536520658	Illumina PCR Primer Index 7 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGCT	480	0.13376509734197606	Illumina PCR Primer Index 7 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	479	0.13348642005584693	Illumina PCR Primer Index 7 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCT	419	0.11676578288809994	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	379	0.10561869144293526	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	35	1.2088094E-7	45.000004	2
CGTGCAC	20	7.026793E-4	45.000004	18
ATATCGG	20	7.026793E-4	45.000004	2
AGTACGA	20	7.026793E-4	45.000004	6
GTTTACG	25	3.885424E-5	45.0	1
CGCATGG	25	3.885424E-5	45.0	2
CACGCCA	50	2.1827873E-11	45.0	27
TTGCGAG	25	3.885424E-5	45.0	1
CGTTATT	140	0.0	43.39286	1
TTTACGG	40	3.4493678E-7	39.375004	2
CGGGAAT	75	0.0	39.000004	6
CGGGTAC	35	6.2362815E-6	38.57143	6
CCGACGG	35	6.2362815E-6	38.57143	2
GCGTATG	35	6.2362815E-6	38.57143	1
AGACACG	95	0.0	37.894737	24
TCAAGCG	95	0.0	37.894737	17
TGGTCAA	95	0.0	37.894737	14
GTATTAG	60	1.546141E-10	37.500004	1
GCGTTAG	30	1.1383573E-4	37.500004	1
CATACGA	30	1.1383573E-4	37.500004	18