##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554378_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	358838
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.151174067406465	33.0	31.0	34.0	30.0	34.0
2	32.278025181279574	34.0	31.0	34.0	30.0	34.0
3	32.13099783189072	33.0	31.0	34.0	30.0	34.0
4	35.87404622698822	37.0	35.0	37.0	35.0	37.0
5	32.947931935859636	37.0	35.0	37.0	28.0	37.0
6	34.280589012311964	37.0	35.0	37.0	27.0	37.0
7	33.96760933903321	37.0	35.0	37.0	32.0	37.0
8	34.910873430350186	37.0	35.0	37.0	32.0	37.0
9	37.38078464376682	39.0	37.0	39.0	33.0	39.0
10	37.146274920716316	39.0	37.0	39.0	34.0	39.0
11	37.18126564076268	39.0	37.0	39.0	34.0	39.0
12	37.23776746052536	39.0	37.0	39.0	34.0	39.0
13	37.18197348106945	39.0	37.0	39.0	34.0	39.0
14	38.3647969278616	40.0	38.0	41.0	34.0	41.0
15	38.43604077606051	40.0	38.0	41.0	34.0	41.0
16	38.4085687691939	40.0	38.0	41.0	34.0	41.0
17	38.289576354789624	40.0	37.0	41.0	34.0	41.0
18	38.19574292577709	40.0	37.0	41.0	34.0	41.0
19	38.04318940580429	40.0	37.0	41.0	34.0	41.0
20	38.019501836483315	40.0	37.0	41.0	34.0	41.0
21	37.942227411812574	40.0	36.0	41.0	33.0	41.0
22	38.01591525981083	40.0	36.0	41.0	34.0	41.0
23	37.837857194611495	40.0	36.0	41.0	33.0	41.0
24	37.81124351378617	40.0	36.0	41.0	33.0	41.0
25	37.63649613474604	39.0	36.0	41.0	33.0	41.0
26	37.69577636705143	40.0	36.0	41.0	33.0	41.0
27	37.71027594624873	40.0	36.0	41.0	33.0	41.0
28	37.62774288118872	40.0	36.0	41.0	33.0	41.0
29	37.53981741064213	40.0	36.0	41.0	33.0	41.0
30	37.380436297159164	40.0	35.0	41.0	33.0	41.0
31	37.32408774990385	40.0	35.0	41.0	33.0	41.0
32	36.96292198708052	39.0	35.0	41.0	31.0	41.0
33	36.9409872978893	39.0	35.0	41.0	31.0	41.0
34	36.84894297705371	39.0	35.0	41.0	31.0	41.0
35	36.74708364220066	39.0	35.0	41.0	31.0	41.0
36	36.66871958934115	39.0	35.0	41.0	31.0	41.0
37	36.464591264024435	39.0	35.0	41.0	30.0	41.0
38	36.343207798505176	39.0	35.0	41.0	30.0	41.0
39	36.21560982950523	39.0	35.0	41.0	29.0	41.0
40	36.14912857612627	39.0	35.0	41.0	29.0	41.0
41	36.070307492517514	39.0	35.0	41.0	29.0	41.0
42	36.06938507070043	39.0	35.0	41.0	29.0	41.0
43	35.956177996756196	39.0	35.0	41.0	29.0	41.0
44	35.80148980877165	39.0	35.0	40.0	28.0	41.0
45	35.696227824254954	38.0	35.0	40.0	28.0	41.0
46	35.54688745339122	38.0	35.0	40.0	27.0	41.0
47	35.39510308272814	38.0	35.0	40.0	27.0	41.0
48	35.2853739013148	38.0	34.0	40.0	26.0	41.0
49	35.18870632430233	38.0	34.0	40.0	26.0	41.0
50	35.02192354209978	37.0	34.0	40.0	26.0	41.0
51	33.312383303886435	36.0	32.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	12.0
10	16.0
11	17.0
12	12.0
13	10.0
14	16.0
15	18.0
16	29.0
17	69.0
18	141.0
19	256.0
20	413.0
21	580.0
22	853.0
23	1253.0
24	1959.0
25	2826.0
26	3761.0
27	4533.0
28	4835.0
29	5290.0
30	6159.0
31	7612.0
32	10078.0
33	14311.0
34	22429.0
35	28734.0
36	32561.0
37	50159.0
38	73287.0
39	86578.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.68736309978319	17.660336976574385	21.724845194767557	13.927454728874869
2	34.61812851481727	25.176820738048928	23.450414950479047	16.754635796654757
3	31.854207190988692	25.519036445415477	26.87563747429202	15.75111888930381
4	28.628517604044163	23.986311371705337	28.806035035308415	18.579135988942085
5	23.77117250681366	34.655471271158575	23.338108004169012	18.235248217858754
6	24.103913186451827	35.92791175962412	26.12711028374921	13.841064770174844
7	73.23666947201801	10.17840919857986	12.374107535991172	4.210813793410955
8	76.71762745305682	6.816167741432066	11.488192443386708	4.978012362124413
9	70.452404706302	7.459633595104198	13.553191133603464	8.53477056499033
10	36.67281614544725	25.314765994682837	21.67997815170077	16.332439708169147
11	29.905974283660036	25.1497890412944	26.32302041589798	18.621216259147584
12	25.980526031245297	22.781589463769166	31.17646403112268	20.061420473862857
13	24.11143747317731	23.71153556758203	33.620185153188906	18.556841806051754
14	21.20232528327546	27.5712159804703	30.648370573908	20.57808816234624
15	20.077583756458345	24.750444490271377	36.05526727938513	19.11670447388515
16	22.496223922772952	23.76559896109108	32.281976825196885	21.456200290939087
17	22.43324285610777	23.277635033078994	31.156677943807516	23.132444167005726
18	22.89807656937114	23.602294071419415	31.687558173883478	21.81207118532597
19	23.690634771122348	25.637752969306483	28.809379162741962	21.862233096829208
20	25.325634408841875	25.05225199114921	30.2100112028269	19.412102397182014
21	23.721010595310418	26.385165450704772	29.871418300180025	20.022405653804782
22	22.509879109793278	23.488593738678734	29.287589385739526	24.71393776578846
23	21.88759272986696	26.539552667220306	29.262229752701774	22.310624850210957
24	21.87783902485244	24.71505247493298	30.09686822465848	23.3102402755561
25	21.948623055529236	26.918275099069778	28.71490756274419	22.4181942826568
26	21.85916764668179	25.594000635384212	29.786700405196775	22.760131312737226
27	23.284880642518353	25.30138948494864	29.220428159782408	22.193301712750603
28	21.553458663798146	25.37997647963705	30.754267942637064	22.312296913927735
29	24.39290153216772	24.73595327139266	28.327824812310848	22.54332038412877
30	24.565124094995515	24.72982237109782	29.413272841783755	21.29178069212291
31	25.904168454845923	25.104643320941484	27.133413963961456	21.857774260251144
32	24.832654289679464	25.95683846192432	28.404182388710225	20.806324859685986
33	23.44539875932872	24.974779705605314	27.057056387562074	24.52276514750389
34	24.16215673925281	26.107045519147913	28.004001805828814	21.72679593577046
35	23.14386993573702	24.771902641303317	29.104498408752693	22.979729014206967
36	25.76538716635362	27.435778819411542	26.509176843032233	20.289657171202606
37	24.006933490878893	26.810984343910064	29.129022009932058	20.053060155278985
38	22.728640779404635	26.69477591559422	27.648688266014187	22.927895038986954
39	22.640021402415574	25.741142242460384	28.709055339735478	22.909781015388557
40	23.154180995323795	24.45950540355258	30.412888267128903	21.97342533399473
41	21.686109051995608	27.917611847128786	28.233353212313077	22.162925888562526
42	23.008990129250524	25.975509840094972	29.621723451808336	21.393776578846165
43	23.888774321560145	25.131675017696008	28.658057396373852	22.321493264369995
44	22.97861430506245	24.605253624198106	28.80046148958583	23.615670581153616
45	22.836488889136604	23.942837715069196	28.476638483103795	24.744034912690406
46	24.247989343380578	25.345141818870907	28.357085927354404	22.049782910394107
47	21.813185894470486	25.167903064892794	31.307163678317234	21.711747362319485
48	22.281921089739658	25.249276832442497	28.905522826456508	23.563279251361337
49	22.61020293279976	24.381197086150298	30.735596564466416	22.273003416583528
50	21.77110562426499	24.23126870621283	29.735145107262888	24.26248056225929
51	21.49521511099716	23.735223136903002	28.09345721467626	26.676104537423573
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	162.0
1	433.0
2	704.0
3	2523.5
4	4343.0
5	3034.5
6	1726.0
7	1578.5
8	1431.0
9	1425.5
10	1420.0
11	1396.0
12	1372.0
13	1453.0
14	1534.0
15	1530.5
16	1527.0
17	1470.0
18	1413.0
19	1440.5
20	1468.0
21	1738.0
22	2008.0
23	2027.5
24	2047.0
25	2245.0
26	2764.5
27	3086.0
28	3751.5
29	4417.0
30	5035.0
31	5653.0
32	6187.5
33	6722.0
34	7450.5
35	8179.0
36	8647.5
37	9116.0
38	10250.5
39	11385.0
40	12815.5
41	14246.0
42	15839.0
43	17432.0
44	19698.0
45	21964.0
46	28563.5
47	35163.0
48	33893.5
49	32624.0
50	32568.5
51	32513.0
52	29176.5
53	25840.0
54	23092.5
55	20345.0
56	19176.0
57	18007.0
58	16848.0
59	15689.0
60	14797.0
61	13905.0
62	12682.5
63	11460.0
64	9988.0
65	8516.0
66	7183.5
67	5851.0
68	5119.5
69	4388.0
70	3741.0
71	3094.0
72	2679.0
73	2264.0
74	1815.0
75	1155.0
76	944.0
77	716.5
78	489.0
79	379.0
80	269.0
81	210.0
82	151.0
83	110.0
84	69.0
85	63.0
86	57.0
87	36.5
88	16.0
89	13.5
90	11.0
91	8.5
92	6.0
93	4.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	358838.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.56789474019638
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.6258276452578	33.25916031393887
2	9.123159971940472	8.13200066654832
3	3.6779626738236164	4.917571599160265
4	2.288335333892614	4.079451531647926
5	1.759377576582054	3.920587732068539
6	1.3548070245453816	3.622853811793033
7	1.1898480331089711	3.7120315327501228
8	1.0037612115554813	3.5788419216717346
9	0.8309504935693907	3.333034271854335
>10	4.082398653288041	25.565771379957415
>50	0.04068568475916092	1.2871421499350677
>100	0.019071414730856683	1.7036288240280788
>500	6.357138243618894E-4	0.1518618076964993
>1k	0.0031785691218094467	2.736062456949802
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTC	2275	0.6339908259437407	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2140	0.5963693923163099	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGC	1992	0.5551251539692006	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCG	1988	0.5540104448246841	No Hit
GCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	1262	0.35169073509494536	Illumina PCR Primer Index 7 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCT	536	0.14937102536520658	Illumina PCR Primer Index 7 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGCT	480	0.13376509734197606	Illumina PCR Primer Index 7 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	479	0.13348642005584693	Illumina PCR Primer Index 7 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCT	419	0.11676578288809994	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	379	0.10561869144293526	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1513217663681104	0.0
2	0.0	0.0	0.0	0.8429987905405782	0.0
3	0.0	0.0	0.0	1.0890708341925883	0.0
4	0.0	0.0	0.0	1.522971368695623	0.0
5	0.0	0.0	0.0	3.0250419409315623	0.0
6	0.0	0.0	0.0	3.806174373951477	0.0
7	0.0	0.0	0.0	4.546062568624282	0.0
8	0.0	0.0	0.0	5.715949815794314	0.0
9	0.0	0.0	0.0	6.2345682452806	0.0
10	0.0	0.0	0.0	7.858142114268834	0.0
11	0.0	0.0	0.0	9.081256723089528	0.0
12	0.0	0.0	0.0	10.751927053433583	0.0
13	0.0	0.0	0.0	11.21954753955824	0.0
14	0.0	0.0	0.0	11.434686404449918	0.0
15	0.0	0.0	0.0	11.851866301785206	0.0
16	0.0	0.0	0.0	12.496725541887983	0.0
17	0.0	0.0	0.0	13.256399823875956	0.0
18	0.0	0.0	0.0	14.096333164269113	0.0
19	0.0	0.0	0.0	14.580674287561518	0.0
20	0.0	0.0	0.0	15.008722599055842	0.0
21	0.0	0.0	0.0	15.551307275149231	0.0
22	0.0	0.0	0.0	16.08525295537262	0.0
23	2.786772861291168E-4	0.0	0.0	16.663229646804407	0.0
24	2.786772861291168E-4	0.0	0.0	17.11998171877003	0.0
25	2.786772861291168E-4	0.0	0.0	17.5427351618279	0.0
26	2.786772861291168E-4	0.0	0.0	17.945702517570602	0.0
27	2.786772861291168E-4	0.0	0.0	18.3227528857033	0.0
28	5.573545722582336E-4	0.0	0.0	18.729343046165678	0.0
29	5.573545722582336E-4	0.0	0.0	19.138441302203223	0.0
30	5.573545722582336E-4	0.0	0.0	19.646191317530473	0.0
31	5.573545722582336E-4	0.0	0.0	20.07005946973286	0.0
32	5.573545722582336E-4	0.0	0.0	20.47107608447266	0.0
33	5.573545722582336E-4	0.0	0.0	20.889649368238594	0.0
34	5.573545722582336E-4	0.0	0.0	21.290665982978393	0.0
35	5.573545722582336E-4	0.0	0.0	21.72986138591788	0.0
36	5.573545722582336E-4	0.0	0.0	22.155401601837042	0.0
37	8.360318583873502E-4	0.0	0.0	22.563106471443938	0.0
38	8.360318583873502E-4	0.0	0.0	22.976663564059546	0.0
39	8.360318583873502E-4	0.0	0.0	23.437595795317108	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	35	1.2088094E-7	45.000004	2
CGTGCAC	20	7.026793E-4	45.000004	18
ATATCGG	20	7.026793E-4	45.000004	2
AGTACGA	20	7.026793E-4	45.000004	6
GTTTACG	25	3.885424E-5	45.0	1
CGCATGG	25	3.885424E-5	45.0	2
CACGCCA	50	2.1827873E-11	45.0	27
TTGCGAG	25	3.885424E-5	45.0	1
CGTTATT	140	0.0	43.39286	1
TTTACGG	40	3.4493678E-7	39.375004	2
CGGGAAT	75	0.0	39.000004	6
CGGGTAC	35	6.2362815E-6	38.57143	6
CCGACGG	35	6.2362815E-6	38.57143	2
GCGTATG	35	6.2362815E-6	38.57143	1
AGACACG	95	0.0	37.894737	24
TCAAGCG	95	0.0	37.894737	17
TGGTCAA	95	0.0	37.894737	14
GTATTAG	60	1.546141E-10	37.500004	1
GCGTTAG	30	1.1383573E-4	37.500004	1
CATACGA	30	1.1383573E-4	37.500004	18
>>END_MODULE