##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554375_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	205334
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.906936990464317	33.0	31.0	34.0	30.0	34.0
2	31.974758198837016	33.0	31.0	34.0	30.0	34.0
3	31.968787439001822	33.0	31.0	34.0	30.0	34.0
4	35.645046606991535	37.0	35.0	37.0	33.0	37.0
5	31.81594864951737	37.0	35.0	37.0	0.0	37.0
6	33.59307761987786	37.0	35.0	37.0	17.0	37.0
7	35.33949078087409	37.0	35.0	37.0	32.0	37.0
8	35.37357670916653	37.0	35.0	37.0	33.0	37.0
9	37.36890139967078	39.0	37.0	39.0	34.0	39.0
10	37.116030467433546	39.0	37.0	39.0	34.0	39.0
11	37.185293229567435	39.0	37.0	39.0	34.0	39.0
12	37.12693952292363	39.0	37.0	39.0	34.0	39.0
13	37.09852240739478	39.0	37.0	39.0	33.0	39.0
14	38.20177369553995	40.0	38.0	41.0	33.0	41.0
15	38.35901993824695	40.0	38.0	41.0	34.0	41.0
16	38.23831416131766	40.0	37.0	41.0	34.0	41.0
17	38.22677686111409	40.0	37.0	41.0	34.0	41.0
18	38.15296541244996	40.0	37.0	41.0	34.0	41.0
19	37.97551793663008	40.0	37.0	41.0	34.0	41.0
20	37.8792601322723	40.0	35.0	41.0	34.0	41.0
21	37.81771163080639	40.0	35.0	41.0	33.0	41.0
22	37.86110921717787	40.0	35.0	41.0	34.0	41.0
23	37.81212073986773	40.0	35.0	41.0	34.0	41.0
24	37.71915026249915	40.0	35.0	41.0	33.0	41.0
25	37.51232138856692	39.0	35.0	41.0	33.0	41.0
26	37.58988769516982	39.0	35.0	41.0	33.0	41.0
27	37.56147544975504	40.0	35.0	41.0	33.0	41.0
28	37.45585728617764	40.0	35.0	41.0	33.0	41.0
29	37.42107006146084	40.0	35.0	41.0	33.0	41.0
30	37.161103373040994	39.0	35.0	41.0	33.0	41.0
31	37.076217285008816	39.0	35.0	41.0	32.0	41.0
32	36.792099700974994	39.0	35.0	41.0	31.0	41.0
33	36.52749666397187	39.0	35.0	41.0	31.0	41.0
34	36.24173298138643	39.0	35.0	41.0	30.0	41.0
35	36.079981883175705	39.0	35.0	41.0	29.0	41.0
36	35.87870006915562	39.0	35.0	41.0	27.0	41.0
37	35.76643419988896	39.0	35.0	41.0	26.0	41.0
38	35.628916789231205	39.0	35.0	41.0	26.0	41.0
39	35.52261680968568	39.0	35.0	41.0	25.0	41.0
40	35.366870562108566	39.0	35.0	41.0	25.0	41.0
41	35.201856487478935	38.0	35.0	41.0	24.0	41.0
42	35.124538556693	38.0	35.0	41.0	24.0	41.0
43	35.01068990035747	38.0	35.0	41.0	23.0	41.0
44	34.918522991808466	37.0	35.0	40.0	23.0	41.0
45	34.810615874623785	37.0	34.0	40.0	23.0	41.0
46	34.63738591757819	37.0	34.0	40.0	23.0	41.0
47	34.47645786864329	37.0	34.0	40.0	23.0	41.0
48	34.423027847312184	36.0	34.0	40.0	23.0	41.0
49	34.35050697887345	36.0	34.0	40.0	24.0	41.0
50	34.24954464433557	36.0	34.0	40.0	24.0	41.0
51	33.44731510611979	35.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	2.0
12	6.0
13	5.0
14	12.0
15	23.0
16	24.0
17	43.0
18	78.0
19	165.0
20	286.0
21	539.0
22	840.0
23	1073.0
24	1607.0
25	2480.0
26	3351.0
27	3850.0
28	3713.0
29	3674.0
30	3652.0
31	4441.0
32	5556.0
33	7853.0
34	12782.0
35	16955.0
36	17598.0
37	25848.0
38	38543.0
39	50296.0
40	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.728588543543694	17.605462319927533	19.774611121392464	17.891338015136316
2	38.56399816883712	23.563559858571885	21.60090389316918	16.27153807942182
3	31.17360008571401	24.017941500189934	29.289352956646248	15.519105457449815
4	27.14309369125425	23.089697760721556	32.219213573981904	17.54799497404229
5	22.494082811419442	35.138359940389805	25.65283878948445	16.714718458706304
6	23.245054399174027	34.6045954396252	28.973282554277418	13.177067606923353
7	71.87898740588504	5.769137113191191	17.868448479063378	4.483427001860384
8	71.50106655497872	7.806305823682392	15.247353093009439	5.4452745283294535
9	65.41488501660709	7.623189535098912	17.80075389365619	9.161171554637809
10	36.86530238538187	24.013071386131863	24.761120905451605	14.360505323034664
11	29.3887032834309	24.346187187703936	29.304450310226265	16.960659218638902
12	25.819396690270484	21.17525592449375	33.23414534368395	19.771202041551813
13	24.439206366213096	23.25284658166694	34.65232255739429	17.655624494725668
14	20.086785432514827	27.588709127567768	32.86255564105311	19.46194979886429
15	19.337761890383472	23.302521745059266	37.988837698578905	19.37087866597836
16	21.235645338813836	22.959665715371052	34.96206181148763	20.842627134327486
17	21.440677140658636	23.103821091489962	33.16450271265353	22.290999055197872
18	22.220382401355838	22.16583712390544	34.09420748633933	21.51957298839939
19	22.939211236327157	23.125736604751282	32.077980266297835	21.857071892623726
20	24.93011386326668	23.38726172966971	33.224892126973614	18.45773228009
21	23.10236005727254	25.294398394810408	32.652653725150245	18.95058782276681
22	20.451069964058558	23.479793896773064	32.84697127606728	23.222164863101096
23	21.40756036506375	24.967613741513826	32.535770987756536	21.08905490566589
24	20.753017035658974	24.391966259849806	33.42505381476034	21.429962889730877
25	20.556751439118703	26.100402271421196	32.03853234242746	21.30431394703264
26	20.128668413414243	24.458686822445383	33.59891688663348	21.813727877506892
27	22.886126993094177	24.667614715536637	31.940643049860228	20.505615241508956
28	19.66844263492651	25.29050230356395	34.21693436060272	20.824120700906814
29	22.628984970828018	23.209502566550107	32.91904896412674	21.242463498495134
30	22.751224833685605	25.670371200093506	31.602657134230082	19.975746831990808
31	23.034665471865353	24.73677033516125	29.274255603066223	22.95430858990718
32	24.989529254775146	25.5812481128308	28.992763010509705	20.436459621884346
33	23.648786854588135	26.190986392901323	27.847312184051347	22.312914568459195
34	21.973954630017435	25.48725491151003	29.934156057934874	22.60463440053766
35	23.9814156447544	25.87199392209766	28.836919360651425	21.309671072496517
36	24.043753104697714	28.566141019022666	26.92345154723524	20.46665432904439
37	23.506579524092455	27.351534572939702	28.485297125658683	20.656588777309164
38	21.28045038814809	29.195846766731275	26.97069165359853	22.553011191522103
39	22.776062415381766	27.996337674228332	27.94033136256051	21.28726854782939
40	23.307878870523147	27.045204398687016	29.22945055373197	20.417466177057868
41	19.95285729591787	28.85299073704306	27.260950451459575	23.933201515579494
42	22.515511313274956	26.721828825231086	28.330427498612014	22.432232362881937
43	23.906902899665912	26.173453982292266	27.875071834182357	22.04457128385947
44	22.016811633728462	25.86274070538732	29.343898234096642	22.776549426787575
45	22.198953899500324	24.6788159778702	28.583186418225914	24.539043704403557
46	23.03661351748858	27.487897766565695	28.151694312680803	21.323794403264927
47	20.76714036642738	27.180593569501397	30.99876299102925	21.05350307304197
48	21.79424742127461	25.716150272239375	29.705747708611334	22.78385459787468
49	22.023629793409764	24.953003399339615	31.351846260239412	21.671520547011212
50	21.089541917071696	25.120048311531455	30.138213836968063	23.652195934428786
51	21.116814555796896	24.622809666202382	28.8490946457966	25.411281132204117
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	144.0
1	327.5
2	511.0
3	2988.5
4	5466.0
5	3590.5
6	1715.0
7	1673.5
8	1632.0
9	1620.0
10	1608.0
11	1526.0
12	1444.0
13	1448.0
14	1452.0
15	1359.5
16	1267.0
17	1181.5
18	1096.0
19	1080.0
20	1064.0
21	1166.0
22	1268.0
23	1324.0
24	1380.0
25	1346.5
26	1564.5
27	1816.0
28	2040.5
29	2265.0
30	2686.5
31	3108.0
32	3494.0
33	3880.0
34	4131.5
35	4383.0
36	4663.0
37	4943.0
38	5492.0
39	6041.0
40	6817.0
41	7593.0
42	8539.5
43	9486.0
44	10985.5
45	12485.0
46	14964.5
47	17444.0
48	17913.5
49	18383.0
50	18202.5
51	18022.0
52	16316.5
53	14611.0
54	13372.5
55	12134.0
56	11113.5
57	10093.0
58	9301.0
59	8509.0
60	7874.5
61	7240.0
62	6730.0
63	6220.0
64	5425.5
65	4631.0
66	3908.5
67	3186.0
68	2673.0
69	2160.0
70	2043.5
71	1927.0
72	1645.0
73	1363.0
74	1116.5
75	703.0
76	536.0
77	445.5
78	355.0
79	259.5
80	164.0
81	117.0
82	70.0
83	54.5
84	39.0
85	22.0
86	5.0
87	8.5
88	12.0
89	6.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	205334.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.47193353268333
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.64610802050024	33.6412868789387
2	8.700556309956633	7.7386112382752
3	3.545928424372509	4.730828796010403
4	2.106969205834684	3.7480397790916262
5	1.5900827894344913	3.5357028061597204
6	1.3217836961759166	3.526936600855192
7	1.0655306846554822	3.317034684952322
8	0.9122169170791536	3.245444008298674
9	0.816943361513864	3.26979457858903
>10	4.234745280126155	27.803481157528708
>50	0.03942354023391301	1.1639572598790262
>100	0.01423627841780192	1.3144437842734276
>500	0.004380393359323668	1.4970730614510992
>1k	0.001095098339830917	1.4673653656968646
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3013	1.4673653656968646	No Hit
GCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	849	0.41347268353024824	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCG	759	0.3696416570076071	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGC	743	0.3618494745146931	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTC	723	0.3521092463985507	No Hit
CTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGCT	382	0.1860383570183214	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	361	0.17581111749637177	No Hit
CCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	312	0.1519475586118227	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGCGAAAAT	244	0.11883078301693827	No Hit
TCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	213	0.10373342943691742	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.19188249388800685	0.0
2	0.0	0.0	0.0	0.9589254580342271	0.0
3	0.0	0.0	0.0	1.206327252184246	0.0
4	0.0	0.0	0.0	1.5676897152931322	0.0
5	0.0	0.0	0.0	2.702426290823731	0.0
6	0.0	0.0	0.0	3.3160606621407074	0.0
7	0.0	0.0	0.0	3.9253119308054196	0.0
8	0.0	0.0	0.0	4.93780864347843	0.0
9	0.0	0.0	0.0	5.424820049285554	0.0
10	0.0	0.0	0.0	6.791374053980344	0.0
11	0.0	0.0	0.0	8.015233716773647	0.0
12	0.0	0.0	0.0	9.37107347054068	0.0
13	0.0	0.0	0.0	9.825455112158727	0.0
14	0.0	0.0	0.0	10.028538868380297	0.0
15	0.0	0.0	0.0	10.427888221142139	0.0
16	0.0	0.0	0.0	11.119931428794063	0.0
17	0.0	0.0	0.0	11.862136811244119	0.0
18	0.0	0.0	0.0	12.7221989538995	0.0
19	0.0	0.0	0.0	13.289080230258993	0.0
20	0.0	0.0	0.0	13.740539803442196	0.0
21	0.0	0.0	0.0	14.286479589351982	0.0
22	0.0	0.0	0.0	14.827549261203698	0.0
23	0.0	0.0	0.0	15.34524238557667	0.0
24	0.0	0.0	0.0	15.778195525339203	0.0
25	0.0	0.0	0.0	16.165369592955866	0.0
26	0.0	0.0	0.0	16.51893987357184	0.0
27	0.0	0.0	0.0	16.914393135087224	0.0
28	0.0	0.0	0.0	17.276242609601915	0.0
29	0.0	0.0	0.0	17.676565985175372	0.0
30	0.0	0.0	0.0	18.151889117243126	0.0
31	0.0	0.0	0.0	18.572179960454672	0.0
32	0.0	0.0	0.0	18.9910097694488	0.0
33	0.0	0.0	0.0	19.386463030964187	0.0
34	0.0	0.0	0.0	19.79555261184217	0.0
35	0.0	0.0	0.0	20.236297934097617	0.0
36	0.0	0.0	0.0	20.63710832107688	0.0
37	0.0	0.0	0.0	21.026230434316773	0.0
38	0.0	0.0	0.0	21.416326570368277	0.0
39	0.0	0.0	0.0	21.834669367956597	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGG	35	1.2054261E-7	45.000004	2
CGCATGG	35	1.2054261E-7	45.000004	2
CACGACC	30	2.1567666E-6	45.000004	27
TGTGCGG	30	2.1567666E-6	45.000004	2
GCGATGT	35	1.2054261E-7	45.000004	9
CCTAATA	20	7.0197415E-4	45.0	26
CTGACTA	20	7.0197415E-4	45.0	22
ACGGGAT	50	2.1827873E-11	45.0	5
CACTCTA	25	3.8795817E-5	45.0	10
TGCGCGC	25	3.8795817E-5	45.0	15
AATGACC	20	7.0197415E-4	45.0	14
CTACGGG	25	3.8795817E-5	45.0	3
TACGAAT	20	7.0197415E-4	45.0	12
AGACCTA	20	7.0197415E-4	45.0	32
CGAATGC	40	6.7684596E-9	45.0	45
CGAATAT	20	7.0197415E-4	45.0	14
CGTAAGG	25	3.8795817E-5	45.0	2
ACCCGTC	20	7.0197415E-4	45.0	39
GCTACGA	20	7.0197415E-4	45.0	10
ATAGTTC	25	3.8795817E-5	45.0	23
>>END_MODULE