FastQCFastQC Report
Sat 18 Jun 2016
SRR3554373_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554373_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480510
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43160.8982123160808307No Hit
GCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC10580.22018272252398494RNA PCR Primer, Index 33 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC8950.18626043162473205Illumina PCR Primer Index 5 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT7430.15462737508064348Illumina PCR Primer Index 5 (96% over 26bp)
CGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG5750.11966452311086137Illumina PCR Primer Index 5 (95% over 24bp)
GAATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTC5120.1065534536221931Illumina PCR Primer Index 5 (95% over 22bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4880.1015587604836528No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATACG207.029183E-445.0000041
TATACGG302.1628548E-645.0000042
CGATACG207.029183E-445.00000410
GCGTATG207.029183E-445.0000041
TAGCCGT253.887406E-545.044
CGTTAGG453.8380676E-1045.02
TAAGCCG253.887406E-545.01
CACGTGA1800.042.543
CGTTTTT21000.042.1071431
ACACGAC2050.041.7073226
CACGACC2050.041.7073227
ACGACCA2000.041.62528
CGGGTCT556.002665E-1140.9090926
AACACGT1950.040.38461741
GCGTTAG451.9243089E-840.01
GACACGA2050.039.51219625
TCGGCGT800.039.3750044
CATACGA750.039.018
CGGTAGT1050.038.5714312
AAACACG2000.038.2540