Basic Statistics
Measure | Value |
---|---|
Filename | SRR3554373_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 480510 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 46 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 4316 | 0.8982123160808307 | No Hit |
GCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC | 1058 | 0.22018272252398494 | RNA PCR Primer, Index 33 (96% over 27bp) |
CCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC | 895 | 0.18626043162473205 | Illumina PCR Primer Index 5 (96% over 25bp) |
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT | 743 | 0.15462737508064348 | Illumina PCR Primer Index 5 (96% over 26bp) |
CGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG | 575 | 0.11966452311086137 | Illumina PCR Primer Index 5 (95% over 24bp) |
GAATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTC | 512 | 0.1065534536221931 | Illumina PCR Primer Index 5 (95% over 22bp) |
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 488 | 0.1015587604836528 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ATATACG | 20 | 7.029183E-4 | 45.000004 | 1 |
TATACGG | 30 | 2.1628548E-6 | 45.000004 | 2 |
CGATACG | 20 | 7.029183E-4 | 45.000004 | 10 |
GCGTATG | 20 | 7.029183E-4 | 45.000004 | 1 |
TAGCCGT | 25 | 3.887406E-5 | 45.0 | 44 |
CGTTAGG | 45 | 3.8380676E-10 | 45.0 | 2 |
TAAGCCG | 25 | 3.887406E-5 | 45.0 | 1 |
CACGTGA | 180 | 0.0 | 42.5 | 43 |
CGTTTTT | 2100 | 0.0 | 42.107143 | 1 |
ACACGAC | 205 | 0.0 | 41.70732 | 26 |
CACGACC | 205 | 0.0 | 41.70732 | 27 |
ACGACCA | 200 | 0.0 | 41.625 | 28 |
CGGGTCT | 55 | 6.002665E-11 | 40.909092 | 6 |
AACACGT | 195 | 0.0 | 40.384617 | 41 |
GCGTTAG | 45 | 1.9243089E-8 | 40.0 | 1 |
GACACGA | 205 | 0.0 | 39.512196 | 25 |
TCGGCGT | 80 | 0.0 | 39.375004 | 4 |
CATACGA | 75 | 0.0 | 39.0 | 18 |
CGGTAGT | 105 | 0.0 | 38.57143 | 12 |
AAACACG | 200 | 0.0 | 38.25 | 40 |