##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554373_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	480510
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0229152358952	33.0	31.0	34.0	30.0	34.0
2	32.0751888618343	33.0	31.0	34.0	30.0	34.0
3	32.07377161765624	33.0	31.0	34.0	30.0	34.0
4	35.71894237372791	37.0	35.0	37.0	33.0	37.0
5	32.22137520551081	37.0	35.0	37.0	0.0	37.0
6	33.8384508126782	37.0	35.0	37.0	17.0	37.0
7	35.49292210359826	37.0	35.0	37.0	32.0	37.0
8	35.59190859711556	37.0	35.0	37.0	33.0	37.0
9	37.62820961062205	39.0	37.0	39.0	35.0	39.0
10	37.22937503902104	39.0	37.0	39.0	34.0	39.0
11	37.25632973299203	39.0	37.0	39.0	34.0	39.0
12	37.22202451561882	39.0	37.0	39.0	34.0	39.0
13	37.24297517221286	39.0	37.0	39.0	34.0	39.0
14	38.29147988595451	40.0	38.0	41.0	33.0	41.0
15	38.461686541383116	40.0	38.0	41.0	34.0	41.0
16	38.32471748766935	40.0	38.0	41.0	34.0	41.0
17	38.36787371750848	40.0	38.0	41.0	34.0	41.0
18	38.309877005681464	40.0	38.0	41.0	34.0	41.0
19	38.194919980853676	40.0	37.0	41.0	34.0	41.0
20	38.23841751472394	40.0	37.0	41.0	34.0	41.0
21	38.11413498158207	40.0	37.0	41.0	34.0	41.0
22	38.21151692992862	40.0	37.0	41.0	34.0	41.0
23	38.20902374560363	40.0	37.0	41.0	34.0	41.0
24	38.11757507648124	40.0	36.0	41.0	34.0	41.0
25	37.87821273230526	40.0	36.0	41.0	34.0	41.0
26	37.981604961395185	40.0	36.0	41.0	34.0	41.0
27	38.00014151630559	40.0	36.0	41.0	34.0	41.0
28	37.90596865830056	40.0	36.0	41.0	34.0	41.0
29	37.85391355018626	40.0	36.0	41.0	34.0	41.0
30	37.63192441364384	40.0	35.0	41.0	33.0	41.0
31	37.587207342198916	40.0	35.0	41.0	33.0	41.0
32	37.4007200682608	40.0	35.0	41.0	33.0	41.0
33	37.2686603816778	40.0	35.0	41.0	33.0	41.0
34	37.06335976358452	40.0	35.0	41.0	32.0	41.0
35	36.91860106969678	40.0	35.0	41.0	31.0	41.0
36	36.80997689954423	40.0	35.0	41.0	31.0	41.0
37	36.750681567501196	40.0	35.0	41.0	31.0	41.0
38	36.60183138748413	39.0	35.0	41.0	31.0	41.0
39	36.5923310649102	39.0	35.0	41.0	31.0	41.0
40	36.534384300014565	39.0	35.0	41.0	30.0	41.0
41	36.4373103577449	39.0	35.0	41.0	30.0	41.0
42	36.49717799837673	39.0	35.0	41.0	31.0	41.0
43	36.44695427774656	39.0	35.0	41.0	31.0	41.0
44	36.36443986597573	39.0	35.0	41.0	30.0	41.0
45	36.27875382406194	39.0	35.0	41.0	30.0	41.0
46	36.185330170027676	39.0	35.0	41.0	30.0	41.0
47	36.124960978959855	39.0	35.0	41.0	30.0	41.0
48	36.09118644773262	39.0	35.0	41.0	30.0	41.0
49	36.05521425152442	39.0	35.0	41.0	30.0	41.0
50	35.98281825560342	39.0	35.0	41.0	30.0	41.0
51	35.13227612328568	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	9.0
10	16.0
11	20.0
12	16.0
13	12.0
14	16.0
15	32.0
16	39.0
17	70.0
18	125.0
19	263.0
20	401.0
21	627.0
22	1003.0
23	1607.0
24	2536.0
25	3883.0
26	5358.0
27	6173.0
28	6122.0
29	6175.0
30	6857.0
31	8407.0
32	11085.0
33	16803.0
34	28698.0
35	34042.0
36	37955.0
37	57925.0
38	95665.0
39	148461.0
40	106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.88485151193523	18.81126303302741	22.243657780275125	16.06022767476223
2	36.49518220224345	23.239474724771597	23.52999937566336	16.735343697321596
3	32.06072714407608	23.633223033859856	28.794614055898943	15.511435766165116
4	28.315539739027283	24.623837173003686	28.127614409689706	18.93300867827933
5	22.513579321970408	35.49541112567896	24.96909533620528	17.021914216145344
6	25.00135272939169	32.59432686104348	27.46456889554848	14.939751514016358
7	77.59651203929158	5.469397098915736	12.26842313375372	4.665667728038959
8	78.39545482924393	5.082516492892968	11.614326444819048	4.907702233044057
9	72.87611079894279	6.834613223450084	14.019687415454413	6.2695885621527125
10	36.33576824623837	29.24705000936505	20.46929304280868	13.947888701587896
11	27.912634492518368	24.631537324925603	29.90426838151131	17.55155980104472
12	25.10249526544713	23.039895111444093	32.605356808391086	19.252252814717696
13	23.90855549312189	23.17454371397057	34.48648311169383	18.430417681213708
14	21.110070550040582	25.14099602505671	34.153295456910364	19.595637967992342
15	20.056606522236788	24.464423216998608	35.714345174918314	19.764625085846287
16	23.90460136105388	24.669621860106968	31.994547459990425	19.43122931884872
17	24.077750723189943	24.25631100289276	31.116730140891967	20.549208133025328
18	25.076273126469793	22.607854154960354	32.385590310295306	19.93028240827454
19	23.66922644690017	25.14599071819525	30.744001165428397	20.44078166947618
20	24.53975983850492	26.007367172379347	30.546086449813743	18.906786539301994
21	23.381823479219996	25.82339597510978	32.00703419283678	18.787746352833448
22	22.99535909762544	23.146032340638072	32.27154481696531	21.587063744771182
23	21.157103910428503	25.67626063973695	32.23637385278142	20.930261597053132
24	21.49382947285176	24.97263324384508	32.100060352542094	21.433476930761067
25	22.32440531934819	26.022975588437287	30.113004932259475	21.539614159955047
26	20.993527710141308	26.197997960500302	30.598114503340202	22.210359826018188
27	22.596407983184534	24.778880772512537	31.29258496181141	21.33212628249152
28	20.993735822355415	25.639632890054315	31.38873280472831	21.97789848286196
29	22.116084993028238	25.666895590102186	30.73421989136542	21.482799525504152
30	24.247986514328527	24.900418305550353	30.930677821481346	19.920917358639777
31	24.55495203013465	25.15160974797611	29.6262304634659	20.66720775842334
32	23.94705625273147	26.21964163076731	29.58065388857672	20.252648227924496
33	24.869409585648583	24.63445089592308	28.986285405090424	21.509854113337912
34	23.10003954132068	26.05627354269422	30.517991300909454	20.32569561507565
35	22.01411000811638	26.910782293812822	30.228507211088218	20.84660048698258
36	24.742877359472224	27.100996857505567	27.47851241389357	20.677613369128633
37	23.366839399804373	26.860627250213316	28.860585627770497	20.911947722211817
38	23.03885455037356	26.76114961187072	29.080768350294477	21.119227487461238
39	23.044265467940313	25.09396266466879	28.81022247195688	23.05154939543402
40	24.2007450417265	24.575763251545233	30.124034879607088	21.099456827121184
41	21.244927264781168	25.581777694532892	29.291377910969597	23.881917129716342
42	22.7198185261493	25.38511165220287	29.36151172712326	22.533558094524565
43	23.130840149008343	24.323531248048948	29.635595513100665	22.910033089842045
44	23.334582006617968	24.4785748475578	29.422696718070384	22.764146427753847
45	22.763313978897422	24.0013735406131	29.380449938606894	23.854862541882582
46	23.06694969927785	24.849222700880315	29.370044327901606	22.71378327194023
47	21.13254666916401	25.820274291898194	31.08863499198768	21.958544046950117
48	21.399346527647708	25.769911136084577	30.129653909387944	22.701088426879775
49	23.19473059873884	24.28336559072652	30.30113837381116	22.22076543672348
50	21.722752908368193	24.534973257580486	30.57418159871803	23.168092235333294
51	21.84012819712389	23.775779900522362	29.034359326548874	25.34973257580487
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	192.0
1	489.5
2	787.0
3	4303.0
4	7819.0
5	5117.5
6	2416.0
7	2362.5
8	2309.0
9	2271.0
10	2233.0
11	2282.5
12	2332.0
13	2199.5
14	2067.0
15	2057.5
16	2048.0
17	2015.5
18	1983.0
19	1933.0
20	1883.0
21	2023.0
22	2163.0
23	2404.5
24	2646.0
25	2969.5
26	3894.0
27	4495.0
28	5187.0
29	5879.0
30	6700.0
31	7521.0
32	9037.0
33	10553.0
34	11698.0
35	12843.0
36	14089.5
37	15336.0
38	16493.0
39	17650.0
40	19258.5
41	20867.0
42	22549.5
43	24232.0
44	26670.5
45	29109.0
46	33791.0
47	38473.0
48	41162.0
49	43851.0
50	44774.0
51	45697.0
52	41233.0
53	36769.0
54	32405.0
55	28041.0
56	25530.0
57	23019.0
58	21459.0
59	19899.0
60	18098.5
61	16298.0
62	14452.5
63	12607.0
64	11501.0
65	10395.0
66	9018.5
67	7642.0
68	6303.0
69	4964.0
70	4204.5
71	3445.0
72	3041.0
73	2637.0
74	2249.5
75	1545.0
76	1228.0
77	890.5
78	553.0
79	404.0
80	255.0
81	181.5
82	108.0
83	87.0
84	66.0
85	44.5
86	23.0
87	18.0
88	13.0
89	8.0
90	3.0
91	3.5
92	4.0
93	2.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	480510.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.85364389857518
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.9462006799152	31.260105076910882
2	9.51587837312485	8.155801263680907
3	4.096227263929356	5.266147934881907
4	2.419720936362417	4.147754373632076
5	1.769259642190401	3.790961133527399
6	1.4655157559019754	3.768161419870468
7	1.1860370057091147	3.557820524523355
8	0.9815333100747302	3.3649823155625804
9	0.9000025933542518	3.471155157905754
>10	4.662546545178344	29.097266254428373
>50	0.037729455527486105	1.0915774276459598
>100	0.01644617292211655	1.349443420800296
>500	0.001934843873190182	0.5648591891982806
>1k	9.67421936595091E-4	1.113964507431765
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4316	0.8982123160808307	No Hit
GCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	1058	0.22018272252398494	RNA PCR Primer, Index 33 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	895	0.18626043162473205	Illumina PCR Primer Index 5 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	743	0.15462737508064348	Illumina PCR Primer Index 5 (96% over 26bp)
CGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	575	0.11966452311086137	Illumina PCR Primer Index 5 (95% over 24bp)
GAATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTC	512	0.1065534536221931	Illumina PCR Primer Index 5 (95% over 22bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	488	0.1015587604836528	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16045451707560718	0.0
2	0.0	0.0	0.0	0.6774052569145284	0.0
3	0.0	0.0	0.0	1.0041414330607064	0.0
4	0.0	0.0	0.0	1.3119393977232523	0.0
5	0.0	0.0	0.0	2.1814322282574765	0.0
6	0.0	0.0	0.0	3.031570622879857	0.0
7	0.0	0.0	0.0	3.7472685271898607	0.0
8	0.0	0.0	0.0	4.93912717737404	0.0
9	0.0	0.0	0.0	5.5461905059207925	0.0
10	0.0	0.0	0.0	6.648144679611247	0.0
11	0.0	0.0	0.0	7.872676947410043	0.0
12	0.0	0.0	0.0	8.89388358202743	0.0
13	0.0	0.0	0.0	9.321346069800837	0.0
14	0.0	0.0	0.0	9.543818026679986	0.0
15	0.0	0.0	0.0	9.854945786768225	0.0
16	0.0	0.0	0.0	10.387296830450978	0.0
17	0.0	0.0	0.0	11.046388212524192	0.0
18	0.0	0.0	0.0	11.727955713720839	0.0
19	0.0	0.0	0.0	12.171859066408608	0.0
20	0.0	0.0	0.0	12.591621402260099	0.0
21	0.0	0.0	0.0	13.08401490083453	0.0
22	0.0	0.0	0.0	13.598052069675969	0.0
23	0.0	0.0	0.0	14.104180974381386	0.0
24	0.0	0.0	0.0	14.5318515743689	0.0
25	0.0	0.0	0.0	14.902707539905517	0.0
26	0.0	0.0	0.0	15.276060852011405	0.0
27	0.0	0.0	0.0	15.694366402364155	0.0
28	0.0	0.0	0.0	16.083120018313874	0.0
29	2.0811221410584586E-4	0.0	0.0	16.517450209152774	0.0
30	2.0811221410584586E-4	0.0	0.0	17.061039312397245	0.0
31	2.0811221410584586E-4	0.0	0.0	17.451249713845705	0.0
32	2.0811221410584586E-4	0.0	0.0	17.871012049697196	0.0
33	2.0811221410584586E-4	0.0	0.0	18.272876735135586	0.0
34	2.0811221410584586E-4	0.0	0.0	18.684522694636946	0.0
35	2.0811221410584586E-4	0.0	0.0	19.116563651120686	0.0
36	2.0811221410584586E-4	0.0	0.0	19.520509458700133	0.0
37	2.0811221410584586E-4	0.0	0.0	19.968575055670016	0.0
38	2.0811221410584586E-4	0.0	0.0	20.419137999209173	0.0
39	2.0811221410584586E-4	0.0	0.0	21.053255915589688	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATACG	20	7.029183E-4	45.000004	1
TATACGG	30	2.1628548E-6	45.000004	2
CGATACG	20	7.029183E-4	45.000004	10
GCGTATG	20	7.029183E-4	45.000004	1
TAGCCGT	25	3.887406E-5	45.0	44
CGTTAGG	45	3.8380676E-10	45.0	2
TAAGCCG	25	3.887406E-5	45.0	1
CACGTGA	180	0.0	42.5	43
CGTTTTT	2100	0.0	42.107143	1
ACACGAC	205	0.0	41.70732	26
CACGACC	205	0.0	41.70732	27
ACGACCA	200	0.0	41.625	28
CGGGTCT	55	6.002665E-11	40.909092	6
AACACGT	195	0.0	40.384617	41
GCGTTAG	45	1.9243089E-8	40.0	1
GACACGA	205	0.0	39.512196	25
TCGGCGT	80	0.0	39.375004	4
CATACGA	75	0.0	39.0	18
CGGTAGT	105	0.0	38.57143	12
AAACACG	200	0.0	38.25	40
>>END_MODULE