FastQCFastQC Report
Sat 18 Jun 2016
SRR3554372_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554372_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327811
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36101.101244314559304No Hit
GCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC15110.46093633221581953No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC12340.3764364222066984No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCG11120.3392198553434754No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGC11070.3376945862097367No Hit
CTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGCT7200.21963875525836535No Hit
CCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC6190.18882831875684464No Hit
TCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC5530.16869476619149448No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGG253.884684E-545.00000433
AGCCGTT253.884684E-545.00000445
TAGCCGT253.884684E-545.00000444
GCTTACG253.884684E-545.0000041
ATGACGG302.1607375E-645.0000042
TTTGCGC302.1607375E-645.00000413
CTATCGT207.025902E-445.037
CGTTATT3050.045.01
ATCGTCT207.025902E-445.039
CCCTTCG207.025902E-445.032
TATCGTC207.025902E-445.038
TCGGTGC207.025902E-445.036
TCTATCG207.025902E-445.036
CGTTTTT23500.043.2765961
CGCGGGT556.002665E-1140.9090924
ACGGGAC556.002665E-1140.9090925
CTCTAAC501.0768417E-940.50000423
CGTTTTA1450.040.3448261
AGGCGAT403.448149E-739.3757
CGAGGAT403.448149E-739.3754