##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554372_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	327811
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07059250604769	33.0	31.0	34.0	30.0	34.0
2	32.201137240666114	33.0	31.0	34.0	30.0	34.0
3	31.902477342127018	33.0	31.0	34.0	30.0	34.0
4	35.76958064250437	37.0	35.0	37.0	35.0	37.0
5	32.71796553501866	37.0	35.0	37.0	26.0	37.0
6	34.160699305392434	37.0	35.0	37.0	26.0	37.0
7	33.88628508500325	37.0	35.0	37.0	32.0	37.0
8	34.95352199895672	37.0	35.0	37.0	32.0	37.0
9	37.49540131356178	39.0	37.0	39.0	34.0	39.0
10	37.00938345571076	39.0	37.0	39.0	33.0	39.0
11	37.10811717727593	39.0	37.0	39.0	34.0	39.0
12	37.236593646949004	39.0	37.0	39.0	34.0	39.0
13	37.17363663818481	39.0	37.0	39.0	34.0	39.0
14	38.322896425074205	40.0	38.0	41.0	34.0	41.0
15	38.44728212293059	40.0	38.0	41.0	34.0	41.0
16	38.39710686950713	40.0	38.0	41.0	34.0	41.0
17	38.336837384956574	40.0	38.0	41.0	34.0	41.0
18	38.17443587921089	40.0	37.0	41.0	34.0	41.0
19	37.94002946819966	39.0	37.0	41.0	34.0	41.0
20	37.835414308854794	39.0	36.0	41.0	34.0	41.0
21	37.745557653647985	39.0	35.0	41.0	33.0	41.0
22	37.79657790617154	39.0	35.0	41.0	34.0	41.0
23	37.61696526352075	39.0	35.0	41.0	33.0	41.0
24	37.62874339177148	39.0	35.0	41.0	33.0	41.0
25	37.441486100222384	39.0	35.0	41.0	33.0	41.0
26	37.53831323537038	39.0	35.0	41.0	33.0	41.0
27	37.57594467543798	39.0	35.0	41.0	33.0	41.0
28	37.450460783805305	39.0	35.0	41.0	33.0	41.0
29	37.44551586127372	39.0	35.0	41.0	33.0	41.0
30	37.255998120868426	39.0	35.0	41.0	33.0	41.0
31	37.03443447596328	39.0	35.0	41.0	32.0	41.0
32	36.56711336715363	39.0	35.0	41.0	31.0	41.0
33	36.34994554789193	39.0	35.0	41.0	30.0	41.0
34	36.03496221908356	39.0	35.0	41.0	29.0	41.0
35	35.85298845981374	39.0	35.0	41.0	26.0	41.0
36	35.67293959019069	39.0	35.0	41.0	25.0	41.0
37	35.45343810915436	39.0	35.0	41.0	23.0	41.0
38	35.31634386887566	39.0	35.0	41.0	23.0	41.0
39	35.30076782048192	39.0	35.0	41.0	23.0	41.0
40	35.24592524350922	39.0	35.0	41.0	23.0	41.0
41	35.19511547812611	39.0	35.0	41.0	23.0	41.0
42	35.12156394995897	39.0	35.0	41.0	22.0	41.0
43	35.0069033680993	39.0	35.0	40.0	22.0	41.0
44	34.87374432218565	39.0	34.0	40.0	21.0	41.0
45	34.84930035904836	39.0	34.0	40.0	21.0	41.0
46	34.721699393858046	38.0	34.0	40.0	20.0	41.0
47	34.61023272556442	38.0	34.0	40.0	20.0	41.0
48	34.51730722886053	38.0	34.0	40.0	20.0	41.0
49	34.45535079664807	38.0	34.0	40.0	20.0	41.0
50	34.30314724033055	38.0	34.0	40.0	20.0	41.0
51	32.713954077196924	36.0	31.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	6.0
10	9.0
11	6.0
12	9.0
13	5.0
14	14.0
15	17.0
16	24.0
17	57.0
18	109.0
19	208.0
20	383.0
21	600.0
22	994.0
23	1565.0
24	2880.0
25	4648.0
26	6585.0
27	6962.0
28	6359.0
29	5715.0
30	5845.0
31	6872.0
32	8449.0
33	12116.0
34	18862.0
35	24685.0
36	28022.0
37	44143.0
38	66086.0
39	75550.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.717974686633454	17.428640283578037	23.25821891272715	18.595166117061353
2	37.61466210712881	22.67129535006452	23.908288617526562	15.805753925280117
3	29.214699933803317	23.094099953936873	32.64716559236877	15.044034519891033
4	25.360039779019004	23.263404827781862	34.25052850575484	17.12602688744429
5	20.536833724310654	34.271577219800434	29.592661625143755	15.598927430745155
6	20.905948854675408	33.66329988926546	32.14321667058152	13.287534585477609
7	71.59674324534564	8.719048476103609	15.8484614610248	3.8357468175259526
8	76.46631748171964	3.8995030673162283	15.07423484873906	4.5599446022250625
9	71.64738218058577	4.667018495413515	17.386847909313598	6.298751414687122
10	34.31916561677308	20.134772780657148	29.28577747543554	16.260284127134234
11	27.09945669913456	25.441184096933906	29.59632227106473	17.863036932866805
12	23.02027692786392	22.243914938790947	36.04088941493727	18.694918718407862
13	22.280521398000676	23.45070787740497	38.50572433505892	15.763046389535434
14	20.338243683097883	26.119318753794108	35.29289743175183	18.249540131356177
15	18.61316429283947	24.385392802560013	40.05051691370942	16.95092599089109
16	20.824194429107017	23.245101598176998	36.0430247917245	19.887679180991487
17	20.818703460225557	23.21154567723475	34.67638364789467	21.293367214645023
18	21.156092992608546	22.94645390179097	37.221752778277725	18.675700327322755
19	20.24581237359332	25.808773958164917	34.60042524503449	19.344988423207273
20	20.803755822714916	25.17029629878192	35.561039745463084	18.464908133040076
21	20.74060968057814	26.14433316758742	35.55524372275488	17.55981342907956
22	19.42369231050819	23.531547141493117	34.515315227371865	22.529445320626827
23	17.813313159106926	26.385630744544876	34.59676459911351	21.20429149723469
24	19.547239110341017	24.347261074216544	35.117491481371886	20.988008334070546
25	18.51676728358719	26.19527715665429	34.171519564627175	21.11643599513134
26	19.023766743641914	24.62119941063601	35.54273651585822	20.812297329863856
27	20.989838657031033	25.035767561186173	34.17579031820165	19.79860346358115
28	17.637602154900232	25.654416721830568	36.076275658840004	20.6317054644292
29	21.00356607923468	24.201445345031132	33.73712291533841	21.057865660395777
30	21.12863814820125	24.783182992639052	33.858534338384004	20.229644520775693
31	20.171379239866873	26.8319244930768	32.32563885897667	20.671057408079655
32	21.306789583021924	28.053054961547964	30.62496377485807	20.015191680572038
33	21.308924959809158	25.138875754626905	31.011161919520696	22.541037366043238
34	20.363563150717944	25.943302695760668	32.3476027345025	21.345531419018887
35	21.001430702447447	26.814231371125434	30.237850468715205	21.946487457711914
36	24.08460972938675	26.634554667171024	29.425492128086	19.85534347535623
37	21.169515360985447	28.314181037244023	31.230495620952315	19.285807980818216
38	19.814161208745283	30.188126694955326	27.991129034718178	22.006583061581217
39	21.148161593113105	26.192531672213565	30.778100795885432	21.881205938787897
40	22.002312308006747	24.682210175985553	31.57673171431099	21.73874580169671
41	21.85985217091556	25.564120789113236	30.045361504037388	22.530665535933817
42	23.100201030471826	25.58272907254485	30.22747863860578	21.089591258377542
43	23.423863140651168	25.277370191970373	30.015161175189363	21.283605492189096
44	20.87605357965413	25.37254698591567	30.510263536000927	23.241135898429278
45	20.750371403034066	24.652009847137528	30.261034559548033	24.336584190280377
46	22.39949239043229	25.6705845746482	30.489519875782083	21.440403159137432
47	19.96241736854468	25.668144144034216	33.10169579422289	21.267742693198215
48	20.77508076300063	25.53422551409196	31.013907403961426	22.676786318945975
49	21.70641009606145	23.29299504897639	32.903105753010124	22.09748910195204
50	20.906253908502155	23.323500431651166	31.793624985128627	23.976620674718056
51	19.92123510193374	23.43698045520132	29.67990701959361	26.961877423271336
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	146.0
1	260.5
2	375.0
3	3851.0
4	7327.0
5	5227.5
6	3128.0
7	3012.0
8	2896.0
9	2870.0
10	2844.0
11	2800.5
12	2757.0
13	2703.5
14	2650.0
15	2623.0
16	2596.0
17	2382.0
18	2168.0
19	2064.5
20	1961.0
21	1971.0
22	1981.0
23	2154.5
24	2328.0
25	2441.5
26	2704.0
27	2853.0
28	3199.5
29	3546.0
30	4274.5
31	5003.0
32	5675.0
33	6347.0
34	6923.0
35	7499.0
36	8461.5
37	9424.0
38	10544.5
39	11665.0
40	12815.5
41	13966.0
42	15628.5
43	17291.0
44	19952.5
45	22614.0
46	27974.5
47	33335.0
48	34781.0
49	36227.0
50	35305.5
51	34384.0
52	29737.0
53	25090.0
54	21123.5
55	17157.0
56	15217.0
57	13277.0
58	11524.5
59	9772.0
60	8404.5
61	7037.0
62	6168.0
63	5299.0
64	4429.0
65	3559.0
66	2993.0
67	2427.0
68	2053.5
69	1680.0
70	1343.0
71	1006.0
72	820.5
73	635.0
74	515.0
75	314.5
76	234.0
77	188.5
78	143.0
79	119.0
80	95.0
81	74.0
82	53.0
83	46.5
84	40.0
85	33.0
86	26.0
87	16.0
88	6.0
89	6.5
90	7.0
91	4.0
92	1.0
93	2.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	327811.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.17645005689461
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.05366483580023	37.12172035662627
2	9.466207568898811	9.120965523425028
3	3.461344070721986	5.002658092585981
4	1.9496920075049173	3.75716958503549
5	1.3360738750397638	3.2183648156587417
6	1.0473560744759638	3.027473856826588
7	0.8598210163942003	2.8996186977928486
8	0.7022297306244131	2.7064748436714874
9	0.6359481636595216	2.75739524507851
>10	3.421910252928997	24.522602254901766
>50	0.04576883020131522	1.417398258506124
>100	0.014826522459580988	1.1978319638602366
>500	0.0019338942338583895	0.5875805225884282
>1k	0.003223157056430649	2.6627459834424863
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3610	1.101244314559304	No Hit
GCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	1511	0.46093633221581953	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC	1234	0.3764364222066984	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCG	1112	0.3392198553434754	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGC	1107	0.3376945862097367	No Hit
CTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGCT	720	0.21963875525836535	No Hit
CCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	619	0.18882831875684464	No Hit
TCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	553	0.16869476619149448	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2431278999179405	0.0
2	0.0	0.0	0.0	1.280615964686969	0.0
3	0.0	0.0	0.0	1.650646256531965	0.0
4	3.0505382674772964E-4	0.0	0.0	2.1454435635167823	0.0
5	3.0505382674772964E-4	0.0	0.0	3.6270900000305053	0.0
6	3.0505382674772964E-4	0.0	0.0	4.415959196000134	0.0
7	3.0505382674772964E-4	0.0	0.0	5.231673128723563	0.0
8	3.0505382674772964E-4	0.0	0.0	6.652308799887741	0.0
9	3.0505382674772964E-4	0.0	0.0	7.178831704854321	0.0
10	3.0505382674772964E-4	0.0	0.0	8.910622279301183	0.0
11	3.0505382674772964E-4	0.0	0.0	10.652784683857467	0.0
12	3.0505382674772964E-4	0.0	0.0	12.350104175881834	0.0
13	3.0505382674772964E-4	0.0	0.0	12.895235364280028	0.0
14	3.0505382674772964E-4	0.0	0.0	13.092910244012556	0.0
15	3.0505382674772964E-4	0.0	0.0	13.523036139726855	0.0
16	3.0505382674772964E-4	0.0	0.0	14.446129019465484	0.0
17	3.0505382674772964E-4	0.0	0.0	15.596487000131173	0.0
18	3.0505382674772964E-4	0.0	0.0	16.770639179283183	0.0
19	3.0505382674772964E-4	0.0	0.0	17.41491286137439	0.0
20	3.0505382674772964E-4	0.0	0.0	18.03569739880602	0.0
21	3.0505382674772964E-4	0.0	0.0	18.818770572067443	0.0
22	3.0505382674772964E-4	0.0	0.0	19.607029660383574	0.0
23	3.0505382674772964E-4	0.0	0.0	20.459350052316733	0.0
24	3.0505382674772964E-4	0.0	0.0	21.07616889000064	0.0
25	3.0505382674772964E-4	0.0	0.0	21.66736320623774	0.0
26	3.0505382674772964E-4	0.0	0.0	22.182904173441404	0.0
27	3.0505382674772964E-4	0.0	0.0	22.72528987739887	0.0
28	3.0505382674772964E-4	0.0	0.0	23.26920085049007	0.0
29	3.0505382674772964E-4	0.0	0.0	23.80518042408583	0.0
30	3.0505382674772964E-4	0.0	0.0	24.422304315596488	0.0
31	3.0505382674772964E-4	0.0	0.0	24.981773033851823	0.0
32	3.0505382674772964E-4	0.0	0.0	25.529954760517494	0.0
33	3.0505382674772964E-4	0.0	0.0	26.101930685669487	0.0
34	6.101076534954593E-4	0.0	0.0	26.624487890888346	0.0
35	6.101076534954593E-4	0.0	0.0	27.205615430842773	0.0
36	6.101076534954593E-4	0.0	0.0	27.732138335809353	0.0
37	6.101076534954593E-4	0.0	0.0	28.30106372269387	0.0
38	6.101076534954593E-4	0.0	0.0	28.831247273581422	0.0
39	6.101076534954593E-4	0.0	0.0	29.417255674763812	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCGG	25	3.884684E-5	45.000004	33
AGCCGTT	25	3.884684E-5	45.000004	45
TAGCCGT	25	3.884684E-5	45.000004	44
GCTTACG	25	3.884684E-5	45.000004	1
ATGACGG	30	2.1607375E-6	45.000004	2
TTTGCGC	30	2.1607375E-6	45.000004	13
CTATCGT	20	7.025902E-4	45.0	37
CGTTATT	305	0.0	45.0	1
ATCGTCT	20	7.025902E-4	45.0	39
CCCTTCG	20	7.025902E-4	45.0	32
TATCGTC	20	7.025902E-4	45.0	38
TCGGTGC	20	7.025902E-4	45.0	36
TCTATCG	20	7.025902E-4	45.0	36
CGTTTTT	2350	0.0	43.276596	1
CGCGGGT	55	6.002665E-11	40.909092	4
ACGGGAC	55	6.002665E-11	40.909092	5
CTCTAAC	50	1.0768417E-9	40.500004	23
CGTTTTA	145	0.0	40.344826	1
AGGCGAT	40	3.448149E-7	39.375	7
CGAGGAT	40	3.448149E-7	39.375	4
>>END_MODULE