FastQCFastQC Report
Sat 18 Jun 2016
SRR3554370_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554370_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271764
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28291.0409767298096877No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTC10330.3801092124048807No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGC9610.3536156370968929No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCG9080.33411342193962407No Hit
GCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC7670.2822301702948146No Hit
CTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGCT3130.11517345932500257No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGG302.1593642E-645.0000042
ACGGGTA302.1593642E-645.0000045
TCGTTTA207.0237706E-445.038
CGCATCG207.0237706E-445.021
GAATAAC207.0237706E-445.09
CGTTCAT253.882921E-545.017
TGCATAG207.0237706E-445.01
AACGGGC502.1827873E-1145.04
CCCCGAT253.882921E-545.040
TAGGGTC207.0237706E-445.05
GACGGGT351.2073542E-745.04
CGTAAGG750.045.02
ACGTAAG207.0237706E-445.01
AGTAACG207.0237706E-445.01
GATAGCG253.882921E-545.01
GTACGAG253.882921E-545.01
CGTTATT1250.043.21
CGTTTTT13100.042.0801541
GCGAGAC1450.041.8965521
CGTTTTA700.041.7857131