FastQCFastQC Report
Sat 18 Jun 2016
SRR3554364_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554364_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366812
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26620.7257123540124096No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTC23840.6499242118578454No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCG22300.6079408525348134No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGC21290.5804063116800977No Hit
GCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGC14970.4081109669258367TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGC7370.2009203624745101TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGCT6940.18919773617002714TruSeq Adapter, Index 16 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCT5250.14312508860124534No Hit
GAACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCT4870.132765558378679No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTC4020.10959292498609642No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTA3760.10250482536012999No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGG207.0269994E-445.02
CTCGTAG207.0269994E-445.01
ATATGCG207.0269994E-445.01
TCCAACG207.0269994E-445.045
GGATCGT302.161447E-644.9999968
TCGTCCC302.161447E-644.99999638
CGTTTTT14500.041.5862051
CGTTATT1600.040.781251
GGACTAA1250.039.6000028
CGGGATA403.4496406E-739.3756
CTCGTCC356.2366635E-638.57142637
TATGCGG356.2366635E-638.5714262
ACGATGG356.2366635E-638.5714262
CGGGACG356.2366635E-638.5714266
GCTAGCG356.2366635E-638.5714261
GCTACGA356.2366635E-638.57142610
CGTTTTA700.038.5714261
ACGGGAT950.037.8947375
AATAGGG1250.037.8000033
TAATACG301.13840724E-437.4999964
Produced by FastQC (version 0.11.5)