##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554364_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	366812
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10317274244027	33.0	31.0	34.0	30.0	34.0
2	32.222083792242344	34.0	31.0	34.0	30.0	34.0
3	32.054553831390464	33.0	31.0	34.0	30.0	34.0
4	35.8103333587778	37.0	35.0	37.0	35.0	37.0
5	32.9740848172906	37.0	35.0	37.0	28.0	37.0
6	34.268131904081656	37.0	35.0	37.0	28.0	37.0
7	33.98187354830267	37.0	35.0	37.0	32.0	37.0
8	34.895131566033825	37.0	35.0	37.0	32.0	37.0
9	37.342551497769975	39.0	37.0	39.0	33.0	39.0
10	37.14592216176134	39.0	37.0	39.0	34.0	39.0
11	37.155101250776966	39.0	37.0	39.0	34.0	39.0
12	37.18799548542577	39.0	37.0	39.0	34.0	39.0
13	37.13793987110564	39.0	37.0	39.0	33.0	39.0
14	38.27387599097085	40.0	38.0	41.0	33.0	41.0
15	38.32821445318037	40.0	38.0	41.0	34.0	41.0
16	38.29269762166996	40.0	37.0	41.0	34.0	41.0
17	38.17056693892239	40.0	37.0	41.0	33.0	41.0
18	38.08304799188685	40.0	37.0	41.0	33.0	41.0
19	37.92706618104097	40.0	37.0	41.0	33.0	41.0
20	37.91063269467738	40.0	36.0	41.0	33.0	41.0
21	37.82589173745679	40.0	36.0	41.0	33.0	41.0
22	37.89773235335812	40.0	36.0	41.0	33.0	41.0
23	37.70191269642215	40.0	36.0	41.0	33.0	41.0
24	37.70190179165349	40.0	36.0	41.0	33.0	41.0
25	37.51255411491445	39.0	35.0	41.0	33.0	41.0
26	37.57990469232195	39.0	35.0	41.0	33.0	41.0
27	37.60308550429102	39.0	35.0	41.0	33.0	41.0
28	37.46740292029705	39.0	35.0	41.0	33.0	41.0
29	37.41971364077511	39.0	35.0	41.0	33.0	41.0
30	37.28382113998452	39.0	35.0	41.0	33.0	41.0
31	37.15174258203112	39.0	35.0	41.0	32.0	41.0
32	36.854879338734825	39.0	35.0	41.0	31.0	41.0
33	36.82666597603132	39.0	35.0	41.0	31.0	41.0
34	36.6889959979499	39.0	35.0	41.0	31.0	41.0
35	36.63905215750848	39.0	35.0	41.0	31.0	41.0
36	36.49160060194323	39.0	35.0	41.0	30.0	41.0
37	36.33123780029007	39.0	35.0	41.0	30.0	41.0
38	36.17993413519732	39.0	35.0	41.0	30.0	41.0
39	36.17948431349029	39.0	35.0	41.0	30.0	41.0
40	36.12932510386792	39.0	35.0	41.0	29.0	41.0
41	36.053975878651734	39.0	35.0	41.0	29.0	41.0
42	35.988958921736476	39.0	35.0	41.0	29.0	41.0
43	35.86954080019192	39.0	35.0	40.0	29.0	41.0
44	35.732037119832505	39.0	35.0	40.0	28.0	41.0
45	35.672060892228174	38.0	35.0	40.0	27.0	41.0
46	35.53115492404829	38.0	35.0	40.0	27.0	41.0
47	35.40354732124358	38.0	35.0	40.0	26.0	41.0
48	35.3249484749681	38.0	35.0	40.0	26.0	41.0
49	35.27794346967929	38.0	35.0	40.0	26.0	41.0
50	35.1434222435471	38.0	34.0	40.0	26.0	41.0
51	33.4128927079812	36.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	15.0
10	11.0
11	20.0
12	11.0
13	4.0
14	19.0
15	21.0
16	39.0
17	78.0
18	148.0
19	229.0
20	390.0
21	592.0
22	900.0
23	1371.0
24	2078.0
25	3116.0
26	4294.0
27	5040.0
28	5274.0
29	5686.0
30	6437.0
31	7897.0
32	10264.0
33	14576.0
34	23715.0
35	28948.0
36	33115.0
37	50119.0
38	75504.0
39	86875.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.80980992988234	16.709104391350337	21.71166701198434	14.769418666782986
2	36.2771665049126	25.113136974799076	22.649204497126593	15.960492023161729
3	32.26721045113028	25.44218836897375	27.132700129766746	15.157901050129222
4	28.1094947820682	24.110443496941212	29.479406344394405	18.300655376596186
5	23.863450486897918	33.79006139384753	23.775939718438874	18.570548400815674
6	23.735592074414143	35.33581235074098	26.995300044709552	13.933295530135329
7	72.02872316063814	9.737140551563199	13.641320349388788	4.592815938409867
8	74.9492928257527	6.616468381623283	12.817465077478381	5.616773715145633
9	68.82435689126855	7.0725603306325855	15.057304559283773	9.045778218815089
10	36.171117629739484	24.862872534159187	22.20047326695964	16.765536569141688
11	28.572674830703466	24.086180386683097	28.023074490474688	19.318070292138753
12	25.602488468207145	22.09306129570461	32.48148915520757	19.82296108088067
13	24.650229545380196	22.058711274440313	34.99449309182906	18.296566088350435
14	20.627733007644242	26.16681024612063	31.917167377294092	21.288289368941037
15	19.678200276981123	23.960775547146767	37.13755275181837	19.22347142405374
16	22.386126953316683	23.25169296533374	32.62434162459243	21.73783845675714
17	21.796451588279556	22.012911246087917	32.615345190451784	23.575291975180747
18	22.970895172458917	22.845217713706205	32.93049300459091	21.253394109243974
19	22.58977350795503	25.13549175054251	30.1579555739725	22.11677916752996
20	24.151881617831478	24.93348091120247	30.71164520244703	20.202992268519022
21	23.50168478675725	26.650436735984645	30.29344732451501	19.554431152743096
22	21.273295312039956	23.569294352420314	29.849623240242956	25.307787095296774
23	20.92052604603993	25.990425613120614	30.686564234539766	22.402484106299685
24	23.100934538673762	23.39263710020392	30.60450585040838	22.901922510713934
25	21.545641909206896	26.14636380489188	28.799766637950775	23.50822764795045
26	21.605345517594845	24.421229403618202	30.272183025637112	23.701242053149844
27	23.39181924255477	24.013936294341516	30.149231759048234	22.445012704055483
28	20.82183788970917	24.437041318168433	32.21977470747958	22.521346084642815
29	23.909795753683085	24.40405439298605	29.454052757270755	22.23209709606011
30	24.877321352627504	23.190626260863876	30.980447749800987	20.951604636707632
31	23.43462045952695	24.082091098437346	29.389714622204288	23.093573819831413
32	24.40132820082222	26.247778153386474	28.786953534780757	20.563940111010545
33	23.816559981679987	22.994067805851497	29.76293032943306	23.42644188303545
34	23.111294068896328	23.376825185653686	30.97090607722757	22.540974668222415
35	23.4010882959118	23.28222631756867	30.245738961647927	23.070946424871597
36	25.194104882064927	24.598704513483746	29.39789319869579	20.809297405755537
37	23.213253655823692	25.52043008407577	30.278453267613926	20.987862992486615
38	21.502022834585564	25.808043357360173	30.704557102821063	21.985376705233197
39	23.351198979313654	23.861814771599622	29.206514508794694	23.58047174029203
40	23.42044426027502	22.011548150005996	32.55891301266043	22.009094577058548
41	22.410390063574802	23.809744501270405	29.49712659345932	24.282738841695473
42	23.991036280165318	23.59164912816375	30.62958681831565	21.78772777335529
43	24.925574953927352	22.937090389627386	29.19370140562468	22.943633250820582
44	22.47663653315595	23.667709889534695	30.213297274898316	23.642356302411045
45	22.380401949772637	22.194203024982826	29.780650578497976	25.644744446746564
46	24.58752712561203	23.278954886972073	29.591180223111568	22.542337764304328
47	21.280110792449538	23.10638692300143	33.25818130268367	22.355320981865372
48	22.049169601866897	23.182447684372377	30.708919010283196	24.05946370347753
49	22.750891464837576	21.8757837802471	32.57772373859088	22.795601016324436
50	21.665594364415558	21.996826712321297	31.705614865380632	24.631964057882513
51	21.16533810235216	21.404425155120336	29.705952913208943	27.724283829318562
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	234.0
1	528.5
2	823.0
3	3152.5
4	5482.0
5	3698.0
6	1914.0
7	1835.0
8	1756.0
9	1723.5
10	1691.0
11	1645.5
12	1600.0
13	1574.0
14	1548.0
15	1473.5
16	1399.0
17	1397.0
18	1395.0
19	1398.5
20	1402.0
21	1420.0
22	1438.0
23	1534.0
24	1630.0
25	1883.0
26	2453.0
27	2770.0
28	3349.5
29	3929.0
30	4538.5
31	5148.0
32	5747.5
33	6347.0
34	7110.5
35	7874.0
36	8536.5
37	9199.0
38	10022.0
39	10845.0
40	12154.0
41	13463.0
42	15216.0
43	16969.0
44	19631.0
45	22293.0
46	30138.5
47	37984.0
48	36824.5
49	35665.0
50	35665.5
51	35666.0
52	31523.5
53	27381.0
54	24158.5
55	20936.0
56	19345.0
57	17754.0
58	16587.0
59	15420.0
60	14709.0
61	13998.0
62	12714.5
63	11431.0
64	9968.5
65	8506.0
66	7173.0
67	5840.0
68	4956.5
69	4073.0
70	3619.5
71	3166.0
72	2750.5
73	2335.0
74	1917.0
75	1180.0
76	861.0
77	658.0
78	455.0
79	365.0
80	275.0
81	200.0
82	125.0
83	90.0
84	55.0
85	47.0
86	39.0
87	34.0
88	29.0
89	22.0
90	15.0
91	13.0
92	11.0
93	8.0
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	366812.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.02646294213204
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.3975701440541	34.70283468162107
2	9.272212478308434	8.53534288048875
3	3.6458835645902266	5.03421374330821
4	2.185518464570658	4.023667384756269
5	1.6364095079715364	3.7659070788402227
6	1.3465666747208238	3.718662069188878
7	1.0561782233564143	3.4028503500320615
8	0.9704717886785073	3.5733907054396727
9	0.826180303876118	3.4223541425886177
>10	3.597529430541692	23.172199002717218
>50	0.038446631534093674	1.2807208315495393
>100	0.022226958855647905	1.8126955465541403
>500	0.0018021858531606409	0.5408225273123701
>1k	0.003003643088601068	3.0143390556029956
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2662	0.7257123540124096	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTC	2384	0.6499242118578454	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCG	2230	0.6079408525348134	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGC	2129	0.5804063116800977	No Hit
GCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGC	1497	0.4081109669258367	TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGC	737	0.2009203624745101	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGCT	694	0.18919773617002714	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCT	525	0.14312508860124534	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCT	487	0.132765558378679	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTC	402	0.10959292498609642	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTA	376	0.10250482536012999	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.21455132329367632	0.0
2	0.0	0.0	0.0	1.1128316412767303	0.0
3	0.0	0.0	0.0	1.4895913983184847	0.0
4	0.0	0.0	0.0	2.0195631549676674	0.0
5	0.0	0.0	0.0	3.790497584593743	0.0
6	0.0	0.0	0.0	4.735941027011112	0.0
7	0.0	0.0	0.0	5.679748754130181	0.0
8	0.0	0.0	0.0	7.126538935476484	0.0
9	0.0	0.0	0.0	7.763104805731547	0.0
10	0.0	0.0	0.0	9.628638103442636	0.0
11	0.0	0.0	0.0	11.177115252499918	0.0
12	0.0	0.0	0.0	13.133430749266655	0.0
13	0.0	0.0	0.0	13.697479907963752	0.0
14	0.0	0.0	0.0	13.93356814935171	0.0
15	0.0	0.0	0.0	14.441457749473845	0.0
16	0.0	0.0	0.0	15.277308266905118	0.0
17	0.0	0.0	0.0	16.213755275181835	0.0
18	0.0	0.0	0.0	17.174738012933055	0.0
19	0.0	0.0	0.0	17.743149079092287	0.0
20	0.0	0.0	0.0	18.282662508314885	0.0
21	0.0	0.0	0.0	18.920046236219097	0.0
22	0.0	0.0	0.0	19.582510932030576	0.0
23	0.0	0.0	0.0	20.279053029889972	0.0
24	0.0	0.0	0.0	20.794575968070838	0.0
25	0.0	0.0	0.0	21.271659596741657	0.0
26	0.0	0.0	0.0	21.693128905270274	0.0
27	0.0	0.0	0.0	22.15521847704001	0.0
28	0.0	0.0	0.0	22.600405657393978	0.0
29	0.0	0.0	0.0	23.088121435503744	0.0
30	0.0	0.0	0.0	23.621364622749528	0.0
31	0.0	0.0	0.0	24.09708515533843	0.0
32	0.0	0.0	0.0	24.56299139613753	0.0
33	0.0	0.0	0.0	25.02644406398918	0.0
34	0.0	0.0	0.0	25.481718155349334	0.0
35	0.0	0.0	0.0	25.96261845304952	0.0
36	0.0	0.0	0.0	26.42034611735712	0.0
37	0.0	0.0	0.0	26.891432123267506	0.0
38	0.0	0.0	0.0	27.357338364066607	0.0
39	0.0	0.0	0.0	27.850233907287656	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGG	20	7.0269994E-4	45.0	2
CTCGTAG	20	7.0269994E-4	45.0	1
ATATGCG	20	7.0269994E-4	45.0	1
TCCAACG	20	7.0269994E-4	45.0	45
GGATCGT	30	2.161447E-6	44.999996	8
TCGTCCC	30	2.161447E-6	44.999996	38
CGTTTTT	1450	0.0	41.586205	1
CGTTATT	160	0.0	40.78125	1
GGACTAA	125	0.0	39.600002	8
CGGGATA	40	3.4496406E-7	39.375	6
CTCGTCC	35	6.2366635E-6	38.571426	37
TATGCGG	35	6.2366635E-6	38.571426	2
ACGATGG	35	6.2366635E-6	38.571426	2
CGGGACG	35	6.2366635E-6	38.571426	6
GCTAGCG	35	6.2366635E-6	38.571426	1
GCTACGA	35	6.2366635E-6	38.571426	10
CGTTTTA	70	0.0	38.571426	1
ACGGGAT	95	0.0	37.894737	5
AATAGGG	125	0.0	37.800003	3
TAATACG	30	1.13840724E-4	37.499996	4
>>END_MODULE