FastQCFastQC Report
Sat 18 Jun 2016
SRR3554363_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554363_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375873
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26940.7167314491862943No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTC22320.593817592644324No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGC21800.579983132600639No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCG21420.5698733348764078No Hit
GCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGC16040.42673988288597475TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGC8210.21842484030510306TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGCT6980.18570102135561745TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTATCTGT6420.17080237207780288No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCT5420.14419764122456255No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG5120.13621622196859046No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCC4630.12317990385050269No Hit
GAACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCT4390.116794768445725No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTA4340.115464531903063No Hit
TCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGC3960.10535473417883168TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTC3930.10455659225323446No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTATC207.027221E-445.043
TAATACG700.045.04
TACGGTA207.027221E-445.030
CTACGAA351.2090095E-745.011
CTACCAA253.8857797E-545.023
CATCGTT253.8857797E-545.036
CCGCATC253.8857797E-545.020
GCGTAAG351.2090095E-745.01
CCCGATC351.2090095E-745.041
CATACGA600.044.99999618
CGGTCTA302.1615906E-644.99999631
CGTTATT1900.040.2631571
TTGACGG451.922308E-840.02
CGAATGC451.922308E-840.045
CGTTTTT13700.039.580291
ATAGCGG403.44995E-739.3752
TACGGGT403.44995E-739.3754
CCCCGAT403.44995E-739.37540
TACGAAT403.44995E-739.37512
GCTACGA403.44995E-739.37510
Produced by FastQC (version 0.11.5)