SRR3554361_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554361_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	238467
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCG	4950	2.075758910037867	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGC	4668	1.9575035539508612	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTC	4355	1.8262484955989717	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3300	1.3838392733585778	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	2107	0.8835604087777345	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTGAGACT	1819	0.7627889812846222	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCT	1184	0.49650475747168366	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTA	936	0.3925071393526148	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	896	0.37573332997857145	TruSeq Adapter, Index 15 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCT	835	0.3501532706831553	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTC	805	0.33757291365262276	No Hit
CTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGCT	704	0.29521904498316326	TruSeq Adapter, Index 14 (95% over 22bp)
GAATAATACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCG	631	0.2646068428755341	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGC	515	0.2159627956908084	No Hit
GAATATGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTC	413	0.17318958178699778	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	402	0.16857678420913585	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGTGAGAC	348	0.1459321415541773	No Hit
TCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	278	0.11657797514960141	TruSeq Adapter, Index 15 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	259	0.1086104156969308	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCC	253	0.1060943442908243	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATG	248	0.1039976181190689	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCC	30	2.1582437E-6	45.000004	34
CGTTAGG	30	2.1582437E-6	45.000004	2
GTGCAAG	40	6.7757355E-9	45.0	1
CGGGTGA	20	7.0220325E-4	45.0	6
CACGGGT	25	3.8814796E-5	45.0	4
GCCGGCC	20	7.0220325E-4	45.0	43
GCGTTAG	25	3.8814796E-5	45.0	1
ACGGGCC	20	7.0220325E-4	45.0	5
CCACTCG	40	6.7757355E-9	45.0	40
GTTCCAC	25	3.8814796E-5	45.0	16
CCAACGC	25	3.8814796E-5	45.0	37
CGACTTT	45	3.8198777E-10	45.0	45
ATACGCC	20	7.0220325E-4	45.0	28
CGATCGA	20	7.0220325E-4	45.0	41
CCACGCT	25	3.8814796E-5	45.0	40
GTGCGTA	20	7.0220325E-4	45.0	44
AACAGTC	20	7.0220325E-4	45.0	2
AATACGG	165	0.0	45.0	5
TGCGTAC	20	7.0220325E-4	45.0	45
AGCACGG	25	3.8814796E-5	45.0	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content