FastQCFastQC Report
Sat 18 Jun 2016
SRR3554360_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554360_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221121
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26781.211101614048417No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCG17540.7932308555044523No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGC17270.7810203463262196No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTC15750.712279702063576No Hit
GCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC10820.48932484929065984TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGCT5620.25415948733951094TruSeq Adapter, Index 13 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCT3680.1664247176885054No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTA3660.16552023552715484No Hit
CCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC3550.16054558363972668TruSeq Adapter, Index 16 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCT3200.14471714581609166No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTC3130.14155145825136464No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2700.12210509178232733No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCA207.0209167E-445.00000416
ACGCCGG207.0209167E-445.00000427
TATATGG207.0209167E-445.0000042
TTGAGGT207.0209167E-445.00000422
TACGAGG406.7720975E-945.0000042
CGGTAGT207.0209167E-445.00000412
TCACGAC253.8805563E-545.025
GTGATAC253.8805563E-545.09
CGTTATT2000.043.8751
TACGGGA750.042.04
GTTATTT2250.042.02
CGTTTTT16400.041.295731
ACGGGAC451.9163053E-840.05
CGAACGG403.441346E-739.3750042
GCGAACG403.441346E-739.3750041
TTTCCGC403.441346E-739.37500433
TCCTAGC403.441346E-739.37500415
GGCGACT403.441346E-739.37500410
AGTACGG403.441346E-739.3750042
CTAATCC403.441346E-739.37500411
Produced by FastQC (version 0.11.5)