##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554360_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	221121
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10217482735697	33.0	31.0	34.0	30.0	34.0
2	32.22623812301862	33.0	31.0	34.0	30.0	34.0
3	31.97477851493074	33.0	31.0	34.0	30.0	34.0
4	35.79753619059248	37.0	35.0	37.0	35.0	37.0
5	32.8589595741698	37.0	35.0	37.0	28.0	37.0
6	34.22872544896233	37.0	35.0	37.0	26.0	37.0
7	33.91708612026899	37.0	35.0	37.0	32.0	37.0
8	34.894238900873276	37.0	35.0	37.0	32.0	37.0
9	37.377463017985626	39.0	37.0	39.0	33.0	39.0
10	37.22590798702973	39.0	37.0	39.0	34.0	39.0
11	37.2123633666635	39.0	37.0	39.0	34.0	39.0
12	37.15881350030074	39.0	37.0	39.0	34.0	39.0
13	37.050085699684786	39.0	37.0	39.0	33.0	39.0
14	38.1924738039354	40.0	37.0	41.0	33.0	41.0
15	38.24680152495692	40.0	37.0	41.0	33.0	41.0
16	38.23669845921464	40.0	37.0	41.0	34.0	41.0
17	38.10780522881137	40.0	37.0	41.0	33.0	41.0
18	37.942194545068084	39.0	37.0	41.0	33.0	41.0
19	37.712591748409245	39.0	36.0	41.0	33.0	41.0
20	37.61288163494196	39.0	35.0	41.0	33.0	41.0
21	37.53889951655429	39.0	35.0	41.0	33.0	41.0
22	37.60389108225813	39.0	35.0	41.0	33.0	41.0
23	37.404990932566335	39.0	35.0	41.0	33.0	41.0
24	37.399147977804006	39.0	35.0	41.0	33.0	41.0
25	37.183998806083544	39.0	35.0	41.0	33.0	41.0
26	37.22322619742132	39.0	35.0	41.0	33.0	41.0
27	37.2569633820397	39.0	35.0	41.0	33.0	41.0
28	37.17118229385721	39.0	35.0	41.0	33.0	41.0
29	37.09340587280268	39.0	35.0	41.0	33.0	41.0
30	36.904174637415714	39.0	35.0	41.0	32.0	41.0
31	36.76900882322349	39.0	35.0	41.0	32.0	41.0
32	36.34609557662999	39.0	35.0	41.0	31.0	41.0
33	36.11119251450563	39.0	35.0	41.0	30.0	41.0
34	35.85019514202631	39.0	35.0	41.0	28.0	41.0
35	35.696415084953486	39.0	35.0	41.0	27.0	41.0
36	35.51609752126664	39.0	35.0	41.0	25.0	41.0
37	35.39858267645317	39.0	35.0	41.0	25.0	41.0
38	35.24847933936623	38.0	35.0	40.0	24.0	41.0
39	35.16382433147462	38.0	35.0	40.0	23.0	41.0
40	35.01231904703759	38.0	35.0	40.0	23.0	41.0
41	34.921346231248954	38.0	34.0	40.0	23.0	41.0
42	34.88673169893407	38.0	34.0	40.0	23.0	41.0
43	34.753551223086	38.0	34.0	40.0	23.0	41.0
44	34.62345503140814	38.0	34.0	40.0	22.0	41.0
45	34.534725331379654	38.0	34.0	40.0	22.0	41.0
46	34.339529036138586	37.0	34.0	40.0	22.0	41.0
47	34.200691024371274	37.0	34.0	40.0	21.0	41.0
48	34.115913911387885	37.0	34.0	40.0	20.0	41.0
49	34.08502584557776	37.0	34.0	40.0	20.0	41.0
50	33.92696758788175	37.0	33.0	40.0	20.0	41.0
51	32.14824010383455	35.0	31.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	8.0
10	10.0
11	12.0
12	7.0
13	7.0
14	11.0
15	15.0
16	24.0
17	44.0
18	103.0
19	201.0
20	345.0
21	480.0
22	656.0
23	1108.0
24	1687.0
25	2753.0
26	3887.0
27	4522.0
28	4440.0
29	4417.0
30	4538.0
31	5196.0
32	6761.0
33	9563.0
34	15292.0
35	19469.0
36	19149.0
37	28410.0
38	41780.0
39	46211.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.41857173221901	15.50056304014544	20.462099936234008	18.61876529140154
2	36.86804961989137	24.68738835298321	22.273777705419203	16.170784321706215
3	30.738826253499212	24.486141072082706	30.26849552959692	14.506537144821161
4	26.734231484119555	22.202775855753185	33.50518494398994	17.55780771613732
5	22.904654012961227	32.78250369707084	26.845030548885003	17.467811741082937
6	22.575874747310294	33.77200718158836	30.028807756839015	13.623310314262326
7	68.51542820446723	9.468119265017796	17.56640029667015	4.450052233844818
8	71.65759923299913	6.952302133221178	15.971346005128414	5.418752628651282
9	66.34195757074181	6.207008832268306	18.391740268902545	9.059293328087337
10	37.616960849489644	22.105091782327325	24.647591137883783	15.630356230299247
11	30.038757060613875	22.128156077441762	29.48611846002867	18.346968401915692
12	26.26073507265253	19.634046517517557	35.310531338045685	18.794687071784228
13	24.227006932855765	20.80037626457912	37.60927275111817	17.363344051446948
14	21.6736537913631	24.62769253033407	33.80185509291293	19.8967985853899
15	19.27903726918746	21.930978966267336	39.92067691444955	18.86930685009565
16	22.547835800308427	21.001623545479625	34.78638392554303	21.664156728668917
17	22.341161626439824	20.802185228901823	34.66020866403463	22.196444480623732
18	22.76717272443594	20.98941303630139	35.554289280529666	20.689124958733
19	23.184591241899234	23.986414677936516	32.340664161251084	20.488329918913173
20	24.029829821681343	24.071436001103468	33.32881092252658	18.569923254688607
21	23.055702533906775	25.88854066325677	33.17640567833901	17.879351124497447
22	21.19699169233135	21.524866475820932	32.97470615635783	24.30343567548989
23	20.714450459250816	25.159980282288885	33.16283844591875	20.96273081254155
24	23.0186187652914	21.98931806567445	32.585326585896404	22.40673658313774
25	20.551643670207714	25.3042451870243	31.447035785836714	22.697075356931272
26	19.550834158673304	23.86973647912229	33.21032375938965	23.36910560281475
27	21.934144653832067	23.40754609467215	32.190067881386206	22.468241370109578
28	19.091357220707213	23.508395855662737	34.430470195051576	22.96977672857847
29	22.67129761533278	23.786524120278038	31.78124194445575	21.76093631993343
30	23.4468910686909	22.866665762184503	33.3229317884778	20.363511380646795
31	23.871093202364317	22.660443829396577	29.894492155878456	23.573970812360653
32	25.52358211115181	24.3197163543942	29.94016850502666	20.216533029427328
33	23.671654885786513	22.674011061816834	30.01569276549943	23.63864128689722
34	21.56421144983968	24.573423600653037	30.603153929296628	23.259211020210653
35	22.83953129734399	23.79285549540749	30.4810488375143	22.88656436973422
36	21.938667064638818	27.41982896242329	28.65082918402142	21.990674788916476
37	23.757580691114818	25.003052627294558	29.044278924208918	22.195087757381703
38	21.528484404466333	25.878591359481913	29.264972571578458	23.327951664473296
39	24.220675557726313	23.862952862912163	28.451390867443614	23.46498071191791
40	22.572256818664894	21.796211124226105	33.01631233577996	22.615219721329048
41	20.087192080354196	25.860953957335575	28.583897504081474	25.467956458228752
42	21.53345905635376	24.731707978889386	30.675512502204676	23.059320462552176
43	22.752248768773658	24.01174017845433	29.31336236721071	23.9226486855613
44	22.428896396090828	22.398596243685585	29.803139457582045	25.36936790264154
45	22.334378010229695	21.86269056308537	29.986749336336217	25.816182090348722
46	23.86476182723486	23.661253340930983	30.1644800810416	22.309504750792552
47	20.365320344969497	23.294033583422653	33.33876022630144	23.001885845306415
48	20.897155855843632	23.85029011265325	31.317694836763582	23.934859194739534
49	22.220865498980196	21.627072960053546	32.95661651313082	23.195445027835436
50	21.345779007873517	21.174379638297584	32.31579090181394	25.16405045201496
51	20.46752682920211	21.218247023123084	30.185735411833342	28.12849073584146
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	106.0
1	326.5
2	547.0
3	3218.5
4	5890.0
5	4002.0
6	2114.0
7	1907.0
8	1700.0
9	1706.0
10	1712.0
11	1732.0
12	1752.0
13	1665.5
14	1579.0
15	1509.5
16	1440.0
17	1364.5
18	1289.0
19	1292.0
20	1295.0
21	1185.0
22	1075.0
23	1009.5
24	944.0
25	1110.0
26	1459.0
27	1642.0
28	1731.0
29	1820.0
30	2081.5
31	2343.0
32	2751.5
33	3160.0
34	3594.5
35	4029.0
36	4479.0
37	4929.0
38	5267.5
39	5606.0
40	6180.0
41	6754.0
42	7906.5
43	9059.0
44	10892.0
45	12725.0
46	17411.5
47	22098.0
48	20950.5
49	19803.0
50	20147.0
51	20491.0
52	18122.0
53	15753.0
54	14418.0
55	13083.0
56	12091.5
57	11100.0
58	10284.0
59	9468.0
60	8813.0
61	8158.0
62	7713.5
63	7269.0
64	6274.0
65	5279.0
66	4584.5
67	3890.0
68	3582.0
69	3274.0
70	2801.0
71	2328.0
72	2046.5
73	1765.0
74	1447.0
75	854.0
76	579.0
77	477.0
78	375.0
79	307.5
80	240.0
81	183.0
82	126.0
83	89.5
84	53.0
85	41.5
86	30.0
87	25.5
88	21.0
89	16.0
90	11.0
91	7.0
92	3.0
93	4.5
94	6.0
95	4.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	221121.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.495804876849824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.48740650069867	35.5634352819278
2	8.762370287764437	8.148269183172035
3	3.4286661650770855	4.782557789978434
4	2.157544640583584	4.0126709848666975
5	1.609651262403461	3.742101550824316
6	1.226733884720831	3.422278760387984
7	1.0787659687374838	3.5110664393142748
8	0.9387963336118755	3.4920072917375826
9	0.8188223612383536	3.4264624263245937
>10	3.4292560427873195	22.42982479070794
>50	0.035992191702723414	1.2402390114527788
>100	0.019995662057068563	1.8696556611930568
>500	9.99783102853428E-4	0.26124974679027796
>1k	0.004998915514267141	4.098181081322226
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2678	1.211101614048417	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCG	1754	0.7932308555044523	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGC	1727	0.7810203463262196	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTC	1575	0.712279702063576	No Hit
GCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC	1082	0.48932484929065984	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGCT	562	0.25415948733951094	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCT	368	0.1664247176885054	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTA	366	0.16552023552715484	No Hit
CCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC	355	0.16054558363972668	TruSeq Adapter, Index 16 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCT	320	0.14471714581609166	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTC	313	0.14155145825136464	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	270	0.12210509178232733	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.28943429163218326	0.0
2	0.0	0.0	0.0	1.2540645167125692	0.0
3	0.0	0.0	0.0	1.5670153445398673	0.0
4	0.0	0.0	0.0	2.0690029440894353	0.0
5	0.0	0.0	0.0	3.7676204431058107	0.0
6	0.0	0.0	0.0	4.519697360268812	0.0
7	0.0	0.0	0.0	5.328304412516224	0.0
8	0.0	0.0	0.0	6.655631984298189	0.0
9	0.0	0.0	0.0	7.121440297393734	0.0
10	0.0	0.0	0.0	9.099995025348113	0.0
11	0.0	0.0	0.0	10.562542680251989	0.0
12	0.0	0.0	0.0	12.71159229562095	0.0
13	0.0	0.0	0.0	13.234835225962255	0.0
14	0.0	0.0	0.0	13.463669212783952	0.0
15	0.0	0.0	0.0	13.998670411222815	0.0
16	0.0	0.0	0.0	14.75029508730514	0.0
17	0.0	0.0	0.0	15.56206782711728	0.0
18	0.0	0.0	0.0	16.526698052197666	0.0
19	0.0	0.0	0.0	17.08205009926692	0.0
20	0.0	0.0	0.0	17.57861080584838	0.0
21	0.0	0.0	0.0	18.1620017999195	0.0
22	0.0	0.0	0.0	18.755342097765478	0.0
23	0.0	0.0	0.0	19.334210681029845	0.0
24	0.0	0.0	0.0	19.788712967108506	0.0
25	0.0	0.0	0.0	20.223768886718133	0.0
26	0.0	0.0	0.0	20.586013992339037	0.0
27	0.0	0.0	0.0	20.975845803881132	0.0
28	0.0	0.0	0.0	21.37879260676281	0.0
29	0.0	0.0	0.0	21.78671406153192	0.0
30	0.0	0.0	0.0	22.269255294612453	0.0
31	0.0	0.0	0.0	22.671749856413456	0.0
32	0.0	0.0	0.0	23.10002215981295	0.0
33	0.0	0.0	0.0	23.52331981132502	0.0
34	0.0	0.0	0.0	23.90048887260821	0.0
35	0.0	0.0	0.0	24.3581568462516	0.0
36	0.0	0.0	0.0	24.78145449776367	0.0
37	0.0	0.0	0.0	25.21108352440519	0.0
38	0.0	0.0	0.0	25.676891837500733	0.0
39	0.0	0.0	0.0	26.171191338678824	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTCA	20	7.0209167E-4	45.000004	16
ACGCCGG	20	7.0209167E-4	45.000004	27
TATATGG	20	7.0209167E-4	45.000004	2
TTGAGGT	20	7.0209167E-4	45.000004	22
TACGAGG	40	6.7720975E-9	45.000004	2
CGGTAGT	20	7.0209167E-4	45.000004	12
TCACGAC	25	3.8805563E-5	45.0	25
GTGATAC	25	3.8805563E-5	45.0	9
CGTTATT	200	0.0	43.875	1
TACGGGA	75	0.0	42.0	4
GTTATTT	225	0.0	42.0	2
CGTTTTT	1640	0.0	41.29573	1
ACGGGAC	45	1.9163053E-8	40.0	5
CGAACGG	40	3.441346E-7	39.375004	2
GCGAACG	40	3.441346E-7	39.375004	1
TTTCCGC	40	3.441346E-7	39.375004	33
TCCTAGC	40	3.441346E-7	39.375004	15
GGCGACT	40	3.441346E-7	39.375004	10
AGTACGG	40	3.441346E-7	39.375004	2
CTAATCC	40	3.441346E-7	39.375004	11
>>END_MODULE