##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554357_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	612854
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.92062220365699	33.0	31.0	34.0	30.0	34.0
2	31.991642381382842	33.0	31.0	34.0	30.0	34.0
3	31.98817336592402	33.0	31.0	34.0	30.0	34.0
4	35.62431835314773	37.0	35.0	37.0	33.0	37.0
5	32.08106172106244	37.0	35.0	37.0	0.0	37.0
6	33.715047955956884	37.0	35.0	37.0	17.0	37.0
7	35.36892963087456	37.0	35.0	37.0	32.0	37.0
8	35.49082489467312	37.0	35.0	37.0	33.0	37.0
9	37.49781350860074	39.0	37.0	39.0	35.0	39.0
10	37.102138519125276	39.0	37.0	39.0	34.0	39.0
11	37.14848560994952	39.0	37.0	39.0	34.0	39.0
12	37.0729439638152	39.0	37.0	39.0	34.0	39.0
13	37.08447199496128	39.0	37.0	39.0	33.0	39.0
14	38.113811772461304	40.0	38.0	41.0	33.0	41.0
15	38.30046797442784	40.0	38.0	41.0	33.0	41.0
16	38.16069406416536	40.0	37.0	41.0	33.0	41.0
17	38.17599134541016	40.0	37.0	41.0	33.0	41.0
18	38.092578330238524	40.0	37.0	41.0	34.0	41.0
19	37.92355764994599	40.0	37.0	41.0	34.0	41.0
20	37.9247177957556	40.0	36.0	41.0	34.0	41.0
21	37.791857440760765	40.0	35.0	41.0	33.0	41.0
22	37.875435258642355	40.0	35.0	41.0	34.0	41.0
23	37.87056134087401	40.0	35.0	41.0	34.0	41.0
24	37.74805581753566	40.0	35.0	41.0	33.0	41.0
25	37.485113909675064	39.0	35.0	41.0	33.0	41.0
26	37.585898109500796	39.0	35.0	41.0	33.0	41.0
27	37.588579009029885	40.0	35.0	41.0	33.0	41.0
28	37.45081373377673	39.0	35.0	41.0	33.0	41.0
29	37.39011901692736	39.0	35.0	41.0	33.0	41.0
30	37.11466842021101	39.0	35.0	41.0	32.0	41.0
31	37.05543897894115	39.0	35.0	41.0	32.0	41.0
32	36.8767651022919	39.0	35.0	41.0	32.0	41.0
33	36.670670339101974	39.0	35.0	41.0	31.0	41.0
34	36.426902655444856	39.0	35.0	41.0	30.0	41.0
35	36.28409865971341	39.0	35.0	41.0	30.0	41.0
36	36.12170924885862	39.0	35.0	41.0	29.0	41.0
37	36.07795984035349	39.0	35.0	41.0	29.0	41.0
38	35.90919860195087	39.0	35.0	41.0	28.0	41.0
39	35.879512901930966	39.0	35.0	41.0	28.0	41.0
40	35.76431743939013	39.0	35.0	41.0	27.0	41.0
41	35.60156089378547	39.0	35.0	41.0	27.0	41.0
42	35.620195021979136	39.0	35.0	41.0	27.0	41.0
43	35.557718477810376	39.0	35.0	41.0	27.0	41.0
44	35.47387469119888	38.0	35.0	41.0	26.0	41.0
45	35.394105284456	38.0	35.0	41.0	26.0	41.0
46	35.27523521099642	38.0	35.0	40.0	26.0	41.0
47	35.194393770783904	38.0	35.0	40.0	26.0	41.0
48	35.11688101897026	38.0	35.0	40.0	26.0	41.0
49	35.046518420374184	37.0	35.0	40.0	26.0	41.0
50	34.95217621162626	37.0	34.0	40.0	25.0	41.0
51	34.09408276685801	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	23.0
10	26.0
11	31.0
12	31.0
13	36.0
14	40.0
15	68.0
16	88.0
17	155.0
18	256.0
19	475.0
20	734.0
21	1239.0
22	1908.0
23	2777.0
24	4287.0
25	6612.0
26	8920.0
27	10270.0
28	10328.0
29	10050.0
30	10658.0
31	13003.0
32	16459.0
33	23380.0
34	39930.0
35	47741.0
36	51260.0
37	73103.0
38	113427.0
39	165442.0
40	92.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.823099792120146	19.089212112509667	22.668041654292868	17.41964644107732
2	38.08916968804969	21.901627467553446	22.992588773182522	17.01661407121435
3	32.320585327011	21.932793128542848	30.12658806175696	15.620033482689188
4	27.70415139658059	24.169867537782245	29.769243571878455	18.35673749375871
5	21.910928214550285	35.1250053030575	26.336452075045607	16.62761440734661
6	24.85241183055018	32.12543281107735	28.68187202824816	14.340283330124304
7	76.214726509087	5.629399498086004	13.910654087270377	4.245219905556625
8	76.88421712185936	5.0225665492923275	13.4423533174296	4.650863011418706
9	71.12803375681646	6.700127599721957	16.188684417495846	5.983154225965728
10	38.67462723585063	24.073107134815146	22.53570997333786	14.716555655996371
11	30.219432360725396	24.011428496836114	28.203780998410714	17.56535814402778
12	27.065336931797784	21.712185936617857	32.28370868102354	18.93876845056082
13	25.64313849628133	21.309316737754834	34.1926462093745	18.854898556589333
14	21.449970139707013	24.473365597679056	34.29005929634138	19.786604966272552
15	20.952788102876053	22.803310413246873	36.41356668961939	19.830334794257688
16	24.711105744598225	23.04512983516466	33.284762765683176	18.95900165455394
17	24.85159597555046	22.323424502410035	31.999954312120018	20.82502520991949
18	26.227780189082555	21.57952791366296	32.279629406024924	19.913062491229557
19	24.995186455501635	23.841893827893756	30.763281303540484	20.39963841306412
20	25.019825276493258	25.11071152346236	30.382929702669802	19.486533497374577
21	24.591664572638834	24.97185300250957	31.571304095265756	18.865178329585838
22	23.492218375012648	22.459835458363656	32.40021277498393	21.647733391639772
23	22.145894454470398	24.708984521598946	32.08366103509155	21.061459988839104
24	22.164659119464016	24.393738149706127	32.193475118054224	21.248127612775637
25	23.86539045188577	24.0282351098304	30.547569241613825	21.558805196670004
26	22.052071129502295	24.12417965779778	30.953375518475852	22.87037369422407
27	23.024407118171702	23.459747346023686	31.942354949139602	21.573490586665013
28	22.078341660493365	24.565230870647824	31.26796920636889	22.088458262489922
29	23.392847236046432	24.699683774602107	30.52929408962004	21.37817489973142
30	24.39096424270707	23.248277730095584	31.691071609225034	20.669686417972308
31	24.58464821964122	24.011591667836058	29.765653809879677	21.638106302643042
32	25.09227320046863	24.801012965567658	28.97623251214808	21.130481321815637
33	24.225672019763273	24.314110701733203	28.944903680158735	22.515313598344793
34	23.1838251851175	24.34217611372366	30.313745198693326	22.160253502465512
35	22.65841456529614	25.49791630633071	28.933808052162508	22.909861076210646
36	23.786252516912672	27.055873013801	27.69272942658447	21.46514504270185
37	23.167344914123102	27.106456023783803	27.033192244808713	22.693006817284378
38	23.03697128516743	26.091042891129046	27.630724446605555	23.24126137709797
39	22.789604049251537	24.235135937760056	28.176041928420148	24.799218084568267
40	24.10362011180477	23.080701113152564	30.023300818792077	22.792377956250593
41	21.12623887581708	24.784369523573314	29.078867071113184	25.01052452949642
42	22.94673772219811	25.20061874443179	28.613340208271467	23.239303325098636
43	22.608484239313114	24.837889611555116	29.734488148890275	22.819138000241495
44	23.276832655085876	24.134948943794118	29.27287739004722	23.315341011072785
45	22.867926129224905	23.55112310599262	28.63438926726431	24.946561497518168
46	22.557248545330534	24.46814412568083	28.87163990118364	24.10296742780499
47	21.64544899764055	25.465118935341856	29.97320732180911	22.91622474520848
48	21.759668697601715	25.09374173946813	29.67444122091069	23.47214834201947
49	23.421075819036837	24.11846867279972	29.51306510196556	22.947390406197886
50	21.236216129779688	23.994458712841883	30.379829453670858	24.389495703707574
51	21.679388565629008	23.874364856882714	29.1349978950941	25.311248682394176
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	374.0
1	905.0
2	1436.0
3	8313.0
4	15190.0
5	9727.0
6	4264.0
7	4239.0
8	4214.0
9	4145.5
10	4077.0
11	3952.5
12	3828.0
13	3732.5
14	3637.0
15	3424.0
16	3211.0
17	3168.5
18	3126.0
19	3105.0
20	3084.0
21	3158.0
22	3232.0
23	3316.5
24	3401.0
25	3909.5
26	4713.0
27	5008.0
28	5662.0
29	6316.0
30	7329.0
31	8342.0
32	9295.5
33	10249.0
34	11778.0
35	13307.0
36	14646.5
37	15986.0
38	17054.5
39	18123.0
40	19410.5
41	20698.0
42	22508.0
43	24318.0
44	26840.0
45	29362.0
46	31730.5
47	34099.0
48	39695.5
49	45292.0
50	48374.5
51	51457.0
52	51861.5
53	52266.0
54	48014.0
55	43762.0
56	40245.0
57	36728.0
58	34314.0
59	31900.0
60	29254.0
61	26608.0
62	24302.0
63	21996.0
64	19873.5
65	17751.0
66	15262.5
67	12774.0
68	10840.0
69	8906.0
70	7817.0
71	6728.0
72	5950.5
73	5173.0
74	4355.5
75	2753.0
76	1968.0
77	1600.5
78	1233.0
79	1036.5
80	840.0
81	630.5
82	421.0
83	275.0
84	129.0
85	86.5
86	44.0
87	31.0
88	18.0
89	17.0
90	16.0
91	9.0
92	2.0
93	3.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	612854.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.43119923052549
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.18948565427083	29.59138676067511
2	9.142962501431628	7.393218769052116
3	3.8822849286034735	4.708963062541004
4	2.3271833329464067	3.76363251921258
5	1.694922943461848	3.4263883603747334
6	1.31707020953491	3.1950436825377535
7	1.11011138922852	3.1418194322181603
8	0.9780346638117119	3.1634491477545073
9	0.8553419897535911	3.112425215816539
>10	5.4390886104932115	33.53406263282921
>50	0.03939462094065314	1.0775588577735769
>100	0.02090326812011892	1.6204014718073447
>500	0.002411915552321414	0.6531989483644653
>1k	4.0198592538690235E-4	0.18073083617349722
>5k	4.0198592538690235E-4	1.4377203028693855
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8846	1.4434106655092405	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1112	0.1814461519383083	No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC	893	0.14571170295045802	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	829	0.1352687589540086	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTC	621	0.10132919096554807	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6317099994452185E-4	0.0	0.0	0.10198187496532617	0.0
2	1.6317099994452185E-4	0.0	0.0	0.41168043286002864	0.0
3	1.6317099994452185E-4	0.0	0.0	0.6357142157838571	0.0
4	1.6317099994452185E-4	0.0	0.0	0.9093519826908203	0.0
5	1.6317099994452185E-4	0.0	0.0	1.5757423464642477	0.0
6	1.6317099994452185E-4	0.0	0.0	2.35129410920056	0.0
7	1.6317099994452185E-4	0.0	0.0	2.985376614984972	0.0
8	1.6317099994452185E-4	0.0	0.0	4.0747062106145995	0.0
9	1.6317099994452185E-4	0.0	0.0	4.6838235534075	0.0
10	1.6317099994452185E-4	0.0	0.0	5.607697755093382	0.0
11	3.263419998890437E-4	0.0	0.0	6.555721264771055	0.0
12	3.263419998890437E-4	0.0	0.0	7.361133320497214	0.0
13	3.263419998890437E-4	0.0	0.0	7.749806642365066	0.0
14	3.263419998890437E-4	0.0	0.0	7.969924321290225	0.0
15	3.263419998890437E-4	0.0	0.0	8.187594435216218	0.0
16	3.263419998890437E-4	0.0	0.0	8.577899467083514	0.0
17	3.263419998890437E-4	0.0	0.0	8.999370159940215	0.0
18	3.263419998890437E-4	0.0	0.0	9.493778289772115	0.0
19	3.263419998890437E-4	0.0	0.0	9.794828784669757	0.0
20	3.263419998890437E-4	0.0	0.0	10.105995881563961	0.0
21	3.263419998890437E-4	0.0	0.0	10.462035003442908	0.0
22	4.895129998335655E-4	0.0	0.0	10.85005564131098	0.0
23	4.895129998335655E-4	0.0	0.0	11.200383778191869	0.0
24	4.895129998335655E-4	0.0	0.0	11.504045009088625	0.0
25	4.895129998335655E-4	0.0	0.0	11.78355693199359	0.0
26	4.895129998335655E-4	0.0	0.0	12.086565478890568	0.0
27	4.895129998335655E-4	0.0	0.0	12.397243062784938	0.0
28	4.895129998335655E-4	0.0	0.0	12.683118654687739	0.0
29	8.158549997226092E-4	0.0	0.0	12.98661671458455	0.0
30	8.158549997226092E-4	0.0	0.0	13.387691032448185	0.0
31	8.158549997226092E-4	0.0	0.0	13.703100575340946	0.0
32	8.158549997226092E-4	0.0	0.0	14.051633831222444	0.0
33	8.158549997226092E-4	0.0	0.0	14.400003916103998	0.0
34	8.158549997226092E-4	0.0	0.0	14.74249984498755	0.0
35	8.158549997226092E-4	0.0	0.0	15.090217245869326	0.0
36	8.158549997226092E-4	0.0	0.0	15.403995078762641	0.0
37	8.158549997226092E-4	0.0	0.0	15.745185639646637	0.0
38	8.158549997226092E-4	0.0	0.0	16.157355585506497	0.0
39	8.158549997226092E-4	0.0	0.0	16.78556393529291	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCACGA	20	7.0307055E-4	45.000004	44
CTATCGG	25	3.888667E-5	45.0	2
TACCGGT	35	1.210683E-7	45.0	40
CGTTATT	525	0.0	42.85714	1
CGTTTTT	3925	0.0	42.13376	1
CGGTCTA	60	3.6379788E-12	41.249996	31
CGTATGG	45	1.925764E-8	40.0	2
GTTAGCG	45	1.925764E-8	40.0	1
ACCGGTA	40	3.4546974E-7	39.375004	41
CACGACG	70	0.0	38.571426	26
CCACCCG	195	0.0	38.076923	45
TCACGAC	65	9.094947E-12	38.07692	25
CTCACGA	65	9.094947E-12	38.07692	24
ACGGTCT	65	9.094947E-12	38.07692	30
CTAGCGG	65	9.094947E-12	38.07692	2
CGTTCAT	30	1.1393002E-4	37.499996	17
CGTAACT	30	1.1393002E-4	37.499996	33
TATAGCG	30	1.1393002E-4	37.499996	1
ATAACGC	30	1.1393002E-4	37.499996	11
CGACTCT	60	1.546141E-10	37.499996	36
>>END_MODULE