FastQCFastQC Report
Sat 18 Jun 2016
SRR3554355_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554355_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281053
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38711.3773202919022391No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC13040.4639694292535572TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC8500.302434060479696TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT6320.22486861908607986TruSeq Adapter, Index 20 (95% over 24bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4670.16616083087531533No Hit
TCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC4540.16153536877386115TruSeq Adapter, Index 23 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC3420.12168523374594827No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG3200.11385752865117967No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGC3140.11172269998897005No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGG207.0241815E-445.0000042
CGTTATT2400.045.0000041
TCTACGG207.0241815E-445.0000042
GCGATCA207.0241815E-445.0000049
TCCCGAC207.0241815E-445.00000430
GGGCATA600.045.0000047
CGGTCTA207.0241815E-445.00000431
TAGGATC207.0241815E-445.00000434
AAAACGG253.8832623E-545.02
AATATAC351.2075543E-745.016
CTACGAA351.2075543E-745.011
TACGAAT351.2075543E-745.012
CTACCGG253.8832623E-545.02
GCTACGA351.2075543E-745.010
CGTTTTT19500.042.0000041
CGACGGG603.6379788E-1241.2500043
GCGATTG501.0750227E-940.59
TAGGGAC1500.040.55
CAATTGG451.9193976E-840.02
TTACGGG451.9193976E-840.03
Produced by FastQC (version 0.11.5)