##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554355_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	281053
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.865189128029233	33.0	31.0	34.0	30.0	34.0
2	31.929885110637496	33.0	31.0	34.0	30.0	34.0
3	31.927753840023055	33.0	31.0	34.0	30.0	34.0
4	35.59624341316407	37.0	35.0	37.0	33.0	37.0
5	31.874162524506055	37.0	35.0	37.0	0.0	37.0
6	33.599203708908995	37.0	35.0	37.0	17.0	37.0
7	35.352321448267766	37.0	35.0	37.0	32.0	37.0
8	35.443809530586755	37.0	35.0	37.0	33.0	37.0
9	37.45248405105087	39.0	37.0	39.0	35.0	39.0
10	37.12544964828698	39.0	37.0	39.0	34.0	39.0
11	37.17521606245086	39.0	37.0	39.0	34.0	39.0
12	37.071726685002474	39.0	37.0	39.0	34.0	39.0
13	37.056448427876596	39.0	37.0	39.0	33.0	39.0
14	38.13148765535326	40.0	37.0	41.0	33.0	41.0
15	38.283160115707716	40.0	37.0	41.0	33.0	41.0
16	38.14243576834262	40.0	37.0	41.0	33.0	41.0
17	38.14911778205534	40.0	37.0	41.0	34.0	41.0
18	38.0989030538724	39.0	37.0	41.0	34.0	41.0
19	37.927825000978466	39.0	37.0	41.0	34.0	41.0
20	37.93031207636994	40.0	36.0	41.0	34.0	41.0
21	37.806862050929894	39.0	35.0	41.0	34.0	41.0
22	37.88204360031738	40.0	35.0	41.0	34.0	41.0
23	37.8851248696865	40.0	35.0	41.0	34.0	41.0
24	37.75966098920844	40.0	35.0	41.0	34.0	41.0
25	37.51342985130918	39.0	35.0	41.0	33.0	41.0
26	37.61280968358281	39.0	35.0	41.0	33.0	41.0
27	37.602167562701695	39.0	35.0	41.0	33.0	41.0
28	37.47731922448791	39.0	35.0	41.0	33.0	41.0
29	37.485798052324654	39.0	35.0	41.0	33.0	41.0
30	37.22334577464037	39.0	35.0	41.0	33.0	41.0
31	37.16622487573518	39.0	35.0	41.0	33.0	41.0
32	36.99838464631226	39.0	35.0	41.0	32.0	41.0
33	36.76993663116921	39.0	35.0	41.0	31.0	41.0
34	36.53075398590301	39.0	35.0	41.0	31.0	41.0
35	36.39814910354986	39.0	35.0	41.0	30.0	41.0
36	36.25663842762753	39.0	35.0	41.0	30.0	41.0
37	36.19674936755701	39.0	35.0	41.0	30.0	41.0
38	36.03906736451844	39.0	35.0	41.0	29.0	41.0
39	36.02140877343419	39.0	35.0	41.0	29.0	41.0
40	35.90882858393257	39.0	35.0	41.0	28.0	41.0
41	35.77585010656353	39.0	35.0	41.0	28.0	41.0
42	35.77206434373588	39.0	35.0	41.0	28.0	41.0
43	35.69710339331016	39.0	35.0	41.0	28.0	41.0
44	35.601039661558495	39.0	35.0	41.0	27.0	41.0
45	35.528921591301284	38.0	35.0	41.0	27.0	41.0
46	35.39797120116135	38.0	35.0	40.0	26.0	41.0
47	35.299367023301656	38.0	35.0	40.0	26.0	41.0
48	35.26316744528612	38.0	35.0	40.0	26.0	41.0
49	35.19391716153181	38.0	35.0	40.0	26.0	41.0
50	35.065144296627324	37.0	35.0	40.0	26.0	41.0
51	34.138760304995856	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	4.0
10	13.0
11	7.0
12	5.0
13	10.0
14	15.0
15	23.0
16	28.0
17	60.0
18	103.0
19	184.0
20	272.0
21	483.0
22	726.0
23	1145.0
24	1883.0
25	2815.0
26	3990.0
27	4690.0
28	4466.0
29	4563.0
30	4826.0
31	5888.0
32	7510.0
33	10740.0
34	18487.0
35	23775.0
36	23132.0
37	33190.0
38	52279.0
39	75691.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.19061885124869	18.039658000448313	22.84337829519699	16.926344853106
2	37.802478536076826	22.027162136678847	23.16538161841361	17.00497770883072
3	32.00890935161696	22.289390257353595	30.304604469619612	15.39709592140984
4	27.924270511255884	24.085492771825955	29.529661665237516	18.460575051680646
5	21.489896923356095	36.29208725756352	25.39912400863894	16.818891810441446
6	23.758863986507883	32.866398864271154	28.733726379010367	14.641010770210599
7	74.07357331179529	5.85120955833953	15.31988628479326	4.755330845071926
8	73.99494045607057	5.419974168573187	14.678014467022235	5.907070908334015
9	68.56144570597004	6.979466506317314	17.494209277253756	6.964878510458881
10	36.87382806801564	24.65335719597371	23.625081390342746	14.8477333456679
11	29.101272713687454	23.46247860723778	28.946141830900224	18.49010684817454
12	25.55994776785873	21.05652670492754	34.577464037032165	18.806061490181566
13	23.181748638157217	23.51656093334709	35.26914852358808	18.032541904907614
14	20.467313994157685	26.288991756003316	33.03682935247089	20.206864897368114
15	19.248682632813026	23.699088783966012	38.1148751303135	18.93735345290746
16	21.619409862196807	22.76759187768855	35.12718241755114	20.4858158425635
17	20.13179008941374	23.01914585505225	32.832953215229864	24.01611084030414
18	22.974314453145848	21.94105738063639	35.03823122329241	20.046396942925355
19	22.590045293948116	23.87556795337534	31.95909668283206	21.575290069844478
20	23.845324547327372	23.848882595097724	32.44761664170103	19.858176215873875
21	22.625269966874576	25.00916197300865	32.56467641334553	19.800891646771248
22	20.765122592535928	22.965063528942938	32.31241082642776	23.957403052093376
23	20.730965333940574	24.461222616374847	32.12917136625476	22.678640683429816
24	21.261826061276697	24.022159521513736	33.23003134640086	21.485983070808707
25	20.867950173099022	24.070193166413453	31.802542580936695	23.259314079550833
26	20.598605956883578	24.109687496664332	32.52447047354058	22.767236072911516
27	21.979484296556166	24.329930653648958	32.20566939331728	21.484915656477604
28	18.895012684440303	25.62932969938054	33.413626611350885	22.06203100482827
29	21.991581658975353	23.377797070303465	31.54138187459305	23.089239396128132
30	22.877535553792345	23.41551237666917	31.937392591432932	21.769559478105553
31	22.58791046528591	25.475266230924415	29.722863659167487	22.21395964462219
32	23.533995367421802	24.980697590845853	29.119418757316236	22.36588828441611
33	22.773996363675177	24.703881474312674	28.756142079963563	23.765980082048582
34	20.67296915528388	23.952065980437855	32.06761713982772	23.307347724450548
35	21.737892852949443	23.80262797408318	30.323106318025427	24.13637285494195
36	23.78234710179219	26.537699295150734	28.442322266618753	21.237631336438323
37	21.640046539264837	26.740863822837685	30.194660793515816	21.424428844381666
38	19.856752996765735	27.73071271254888	28.312453523001004	24.100080767684386
39	21.559278854877906	24.36515532657541	29.99398689926811	24.08157891927857
40	22.322480101617845	22.075195781578564	32.447260836923995	23.155063279879595
41	20.06632201043931	24.710285960299302	30.713779963209788	24.5096120660516
42	22.26306070385301	24.840510508694088	29.859848498325935	23.036580289126963
43	22.375139208619014	24.44485559663124	29.574137262366882	23.60586793238286
44	21.481357608707256	24.061653851764614	28.935111882812137	25.521876656715992
45	21.63364205327821	22.550195158920204	29.622526712043634	26.193636075757954
46	22.06914710036897	23.647497091295946	30.382169911013225	23.90118589732186
47	19.94534838624743	24.67506128737284	33.03576193813978	22.34382838823994
48	20.11435565533903	23.688058835877932	31.63104467840585	24.56654083037719
49	21.207032125613317	22.977872500916195	32.883121688791796	22.93197368467869
50	20.764766787758894	21.939278356751217	32.45188629902545	24.84406855646444
51	20.03501119006024	22.472985522303624	29.590860086887528	27.901143200748614
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	135.0
1	382.0
2	629.0
3	3747.0
4	6865.0
5	4450.0
6	2035.0
7	1951.0
8	1867.0
9	1820.0
10	1773.0
11	1722.5
12	1672.0
13	1630.5
14	1589.0
15	1526.0
16	1463.0
17	1486.0
18	1509.0
19	1387.0
20	1265.0
21	1373.0
22	1481.0
23	1537.5
24	1594.0
25	1714.0
26	2097.5
27	2361.0
28	2752.0
29	3143.0
30	3310.0
31	3477.0
32	4200.0
33	4923.0
34	5537.0
35	6151.0
36	6475.0
37	6799.0
38	6758.0
39	6717.0
40	8323.0
41	9929.0
42	11525.0
43	13121.0
44	15006.5
45	16892.0
46	19891.5
47	22891.0
48	25706.5
49	28522.0
50	28570.5
51	28619.0
52	25045.5
53	21472.0
54	18994.5
55	16517.0
56	15050.5
57	13584.0
58	12523.0
59	11462.0
60	10934.5
61	10407.0
62	9609.0
63	8811.0
64	7820.5
65	6830.0
66	5539.0
67	4248.0
68	3468.0
69	2688.0
70	2341.5
71	1995.0
72	1795.0
73	1595.0
74	1240.0
75	766.0
76	647.0
77	485.0
78	323.0
79	250.5
80	178.0
81	129.0
82	80.0
83	52.0
84	24.0
85	27.0
86	30.0
87	24.5
88	19.0
89	10.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	281053.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.075963801894524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.60707029697816	27.26508216587457
2	10.934770390608348	8.327038431496629
3	4.182200112913543	4.777239003347259
4	2.2544070600985378	3.4335488646018946
5	1.508074652960733	2.8710697948343755
6	1.154247672722551	2.636945556300291
7	0.9709680592276039	2.5879381273162214
8	0.8205870985289073	2.4995715727910666
9	0.707058401190012	2.422973708058268
>10	5.753063739757704	36.4196735123697
>50	0.06572653695293178	1.7139026686566035
>100	0.03784255157896072	2.520801978148031
>500	0.0019917132409979325	0.5619477333958156
>1k	0.0019917132409979325	1.962266882809275
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3871	1.3773202919022391	No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	1304	0.4639694292535572	TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	850	0.302434060479696	TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT	632	0.22486861908607986	TruSeq Adapter, Index 20 (95% over 24bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	467	0.16616083087531533	No Hit
TCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	454	0.16153536877386115	TruSeq Adapter, Index 23 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC	342	0.12168523374594827	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	320	0.11385752865117967	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGC	314	0.11172269998897005	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.23269632418084846	0.0
2	0.0	0.0	0.0	1.2858784642042604	0.0
3	0.0	0.0	0.0	1.6705034281790268	0.0
4	0.0	0.0	0.0	2.1519072915072956	0.0
5	0.0	0.0	0.0	3.4911564722667965	0.0
6	0.0	0.0	0.0	4.323383845751513	0.0
7	0.0	0.0	0.0	5.15347639057402	0.0
8	0.0	0.0	0.0	6.601957637883246	0.0
9	0.0	0.0	0.0	7.215009268714442	0.0
10	0.0	0.0	0.0	8.551767816034697	0.0
11	0.0	0.0	0.0	10.195585885936104	0.0
12	0.0	0.0	0.0	11.544797600452585	0.0
13	0.0	0.0	0.0	12.078504766004988	0.0
14	0.0	0.0	0.0	12.296613094327405	0.0
15	0.0	0.0	0.0	12.73745521307369	0.0
16	0.0	0.0	0.0	13.61522559801888	0.0
17	0.0	0.0	0.0	14.694737291542877	0.0
18	0.0	0.0	0.0	15.836870625825023	0.0
19	0.0	0.0	0.0	16.476251810156803	0.0
20	0.0	0.0	0.0	17.04696267252084	0.0
21	0.0	0.0	0.0	17.687055466406694	0.0
22	0.0	0.0	0.0	18.378739952962608	0.0
23	0.0	0.0	0.0	19.024881428058052	0.0
24	0.0	0.0	0.0	19.558232788833422	0.0
25	0.0	0.0	0.0	20.03501119006024	0.0
26	0.0	0.0	0.0	20.476564918360594	0.0
27	0.0	0.0	0.0	20.975403215763574	0.0
28	0.0	0.0	0.0	21.460720931639226	0.0
29	0.0	0.0	0.0	21.946038647514882	0.0
30	0.0	0.0	0.0	22.478678398736182	0.0
31	0.0	0.0	0.0	22.965419333719975	0.0
32	0.0	0.0	0.0	23.452160268703768	0.0
33	0.0	0.0	0.0	23.956335637762272	0.0
34	0.0	0.0	0.0	24.46229003070595	0.0
35	0.0	0.0	0.0	24.96504218065632	0.0
36	0.0	0.0	0.0	25.42794419557877	0.0
37	0.0	0.0	0.0	25.897962306041922	0.0
38	0.0	0.0	0.0	26.392886750897517	0.0
39	0.0	0.0	0.0	26.930507768997305	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGG	20	7.0241815E-4	45.000004	2
CGTTATT	240	0.0	45.000004	1
TCTACGG	20	7.0241815E-4	45.000004	2
GCGATCA	20	7.0241815E-4	45.000004	9
TCCCGAC	20	7.0241815E-4	45.000004	30
GGGCATA	60	0.0	45.000004	7
CGGTCTA	20	7.0241815E-4	45.000004	31
TAGGATC	20	7.0241815E-4	45.000004	34
AAAACGG	25	3.8832623E-5	45.0	2
AATATAC	35	1.2075543E-7	45.0	16
CTACGAA	35	1.2075543E-7	45.0	11
TACGAAT	35	1.2075543E-7	45.0	12
CTACCGG	25	3.8832623E-5	45.0	2
GCTACGA	35	1.2075543E-7	45.0	10
CGTTTTT	1950	0.0	42.000004	1
CGACGGG	60	3.6379788E-12	41.250004	3
GCGATTG	50	1.0750227E-9	40.5	9
TAGGGAC	150	0.0	40.5	5
CAATTGG	45	1.9193976E-8	40.0	2
TTACGGG	45	1.9193976E-8	40.0	3
>>END_MODULE