##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554347_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	495103
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.963221794252913	33.0	31.0	34.0	30.0	34.0
2	32.048297021023906	33.0	31.0	34.0	30.0	34.0
3	32.01052508265957	33.0	31.0	34.0	30.0	34.0
4	35.70458470257704	37.0	35.0	37.0	33.0	37.0
5	33.729977398642305	37.0	35.0	37.0	32.0	37.0
6	34.52129758858258	37.0	35.0	37.0	30.0	37.0
7	35.65565548986776	37.0	35.0	37.0	32.0	37.0
8	35.56869378694938	37.0	35.0	37.0	33.0	37.0
9	37.49636136319109	39.0	37.0	39.0	34.0	39.0
10	36.949461021242044	39.0	37.0	39.0	33.0	39.0
11	37.070017753881515	39.0	37.0	39.0	34.0	39.0
12	36.952516951018275	39.0	37.0	39.0	33.0	39.0
13	37.10161925902287	39.0	37.0	39.0	33.0	39.0
14	38.027325627192724	40.0	37.0	41.0	33.0	41.0
15	38.15201685305886	40.0	37.0	41.0	33.0	41.0
16	38.04032090292323	40.0	37.0	41.0	33.0	41.0
17	38.033744493570026	40.0	37.0	41.0	33.0	41.0
18	37.8325176781397	39.0	37.0	41.0	33.0	41.0
19	37.62736642678392	39.0	36.0	41.0	33.0	41.0
20	37.88440789088331	39.0	36.0	41.0	34.0	41.0
21	37.70746693112342	39.0	35.0	41.0	33.0	41.0
22	37.93379761382985	40.0	36.0	41.0	34.0	41.0
23	37.951894858241616	40.0	36.0	41.0	34.0	41.0
24	37.764745921555715	40.0	35.0	41.0	33.0	41.0
25	37.40258289689216	39.0	35.0	41.0	33.0	41.0
26	37.62584149156842	39.0	35.0	41.0	33.0	41.0
27	37.21742950456774	39.0	35.0	41.0	33.0	41.0
28	37.287382625433494	39.0	35.0	41.0	33.0	41.0
29	37.464119587237406	40.0	35.0	41.0	33.0	41.0
30	37.19200853155808	39.0	35.0	41.0	33.0	41.0
31	37.0685352340826	39.0	35.0	41.0	32.0	41.0
32	37.10242515193808	39.0	35.0	41.0	33.0	41.0
33	37.06008244749072	40.0	35.0	41.0	32.0	41.0
34	36.8198799037776	40.0	35.0	41.0	31.0	41.0
35	36.73215876292408	40.0	35.0	41.0	31.0	41.0
36	36.570166207839584	39.0	35.0	41.0	31.0	41.0
37	36.591773832919614	39.0	35.0	41.0	31.0	41.0
38	36.359511051235806	39.0	35.0	41.0	30.0	41.0
39	36.318965952539166	39.0	35.0	41.0	30.0	41.0
40	36.19467262367628	39.0	35.0	41.0	30.0	41.0
41	36.015265510408945	39.0	35.0	41.0	29.0	41.0
42	36.14746426501152	39.0	35.0	41.0	30.0	41.0
43	36.09854515121096	39.0	35.0	41.0	30.0	41.0
44	36.08724649214406	39.0	35.0	41.0	30.0	41.0
45	35.9569119960897	39.0	35.0	41.0	30.0	41.0
46	35.922723150536356	39.0	35.0	41.0	30.0	41.0
47	35.88579952050382	38.0	35.0	41.0	30.0	41.0
48	35.82665021217807	38.0	35.0	40.0	30.0	41.0
49	35.82088979464879	38.0	35.0	40.0	30.0	41.0
50	35.72732138565107	38.0	35.0	40.0	29.0	41.0
51	34.850909810685856	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	18.0
10	17.0
11	22.0
12	20.0
13	24.0
14	31.0
15	43.0
16	62.0
17	82.0
18	173.0
19	289.0
20	477.0
21	707.0
22	1137.0
23	1803.0
24	2593.0
25	4180.0
26	5604.0
27	6802.0
28	6863.0
29	6847.0
30	7728.0
31	9735.0
32	12760.0
33	18661.0
34	33547.0
35	38041.0
36	41764.0
37	61783.0
38	103466.0
39	129790.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.69918986554313	18.034025243232215	22.090554894638085	16.17622999658657
2	35.710347139888064	22.445632524949353	23.701734790538534	18.14228554462405
3	32.00586544617989	22.71850503834556	28.273510764426796	17.002118751047764
4	28.33349828217563	24.937841216878105	27.57204056529651	19.156619935649754
5	23.279398428205848	32.257732229455286	25.933997572222346	18.52887177011652
6	25.632444158084276	33.04766886890202	27.06608523882909	14.253801734184604
7	79.0092162640905	5.444725642947023	11.402475848459815	4.143582244502659
8	80.40306764451033	4.655192959848758	10.68383750451926	4.257901891121645
9	75.12416608261312	6.120746592123255	13.129187260024683	5.62590006523895
10	38.82485058664561	26.221008557815246	19.68196516684407	15.27217568869508
11	29.96083643201516	25.181628873183964	26.62294512454984	18.23458957025104
12	26.889354336370413	23.18143901370018	31.056568027258972	18.872638622670436
13	25.967929905494415	23.78010232214307	32.146846211798355	18.105121560564168
14	22.040060351078463	25.159815230366206	33.00767719040281	19.792447228152525
15	21.139439672149027	24.093774426735447	34.92121841313828	19.84556748797725
16	25.652843953682364	22.725372296269665	31.927093958226877	19.694689791821098
17	24.814836508766863	23.150132396693213	30.57646590709408	21.458565187445842
18	26.84653496343185	21.58500352451914	31.30459722522384	20.263864286825164
19	25.387444632732986	25.146484670866464	29.03517045948015	20.430900236920397
20	26.472471384742164	24.93440758791605	29.424180423063483	19.1689406042783
21	25.361995382778936	25.557712233616037	30.282587663577075	18.797704720027955
22	24.692639713352573	22.968957974401285	30.453865155331318	21.884537156914824
23	22.63104848890029	25.168500291858464	30.783089579340057	21.41736163990119
24	23.005112067589977	24.163860853196205	31.660684746406304	21.170342332807518
25	23.781718147536974	24.868158746765825	29.622522990165685	21.727600115531516
26	21.474521463210685	24.592458538930284	30.293898441334427	23.639121556524604
27	22.170740229810768	25.03479074051258	30.40801610977918	22.386452919897476
28	21.10429546983153	25.48439415636746	30.485575728686758	22.925734645114247
29	22.443006808684256	24.952383645423275	29.206851907582866	23.397757638309606
30	24.288279408527114	23.959458940866853	30.76248780556773	20.989773845038307
31	24.503992098613825	25.7899871339903	28.294718472721836	21.411302294674037
32	24.08569529976591	24.89158821497749	28.89277584664201	22.129940638614592
33	25.024893809974895	24.46177866019798	27.799871945837534	22.713455583989596
34	22.03622276576793	25.74191632852154	30.14241481065556	22.07944609505497
35	21.929174333421532	26.262818039882614	29.11535579465283	22.69265183204303
36	23.5724687590259	28.220188526427837	27.29270475032468	20.914637964221587
37	22.921291125281	28.471247397006277	27.27937419082494	21.32808728688778
38	22.710829867724495	27.96468613601614	27.62637269416667	21.698111302092695
39	23.352312549105942	25.629818441819175	27.589410688281024	23.428458320793855
40	24.406234662282394	24.84614312577383	29.398933151283675	21.348689060660107
41	21.80071621460585	25.37249824783934	28.368440506318887	24.458345031235925
42	23.187296380753093	25.267065640886845	28.1040510762407	23.441586902119358
43	23.065301563513046	24.636287802740036	28.70776383903955	23.590646794707364
44	22.606407151643193	24.638307584482423	29.042845630101212	23.712439633773176
45	22.18750441827256	24.53671256284046	28.135155715073427	25.140627303813552
46	22.6783113816721	25.400169257710008	28.552240644875916	23.369278715741977
47	21.24083271561675	26.316948190578525	30.089698507179314	22.35252058662541
48	22.015217035647126	26.491457333120582	29.250479193218382	22.242846438013906
49	23.679315213198063	24.6623429872168	29.291480762588794	22.36686103699634
50	21.600555843935503	25.00954346873277	29.772592773624883	23.617307913706846
51	21.87464022637714	24.360991551252972	28.85843955702147	24.905928665348423
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	149.0
1	548.0
2	947.0
3	3847.5
4	6748.0
5	4594.5
6	2441.0
7	2383.5
8	2326.0
9	2359.0
10	2392.0
11	2371.5
12	2351.0
13	2366.5
14	2382.0
15	2313.5
16	2245.0
17	2143.5
18	2042.0
19	2029.0
20	2016.0
21	2066.5
22	2117.0
23	2255.0
24	2393.0
25	2779.5
26	3718.0
27	4270.0
28	4699.5
29	5129.0
30	5912.5
31	6696.0
32	7672.5
33	8649.0
34	10367.0
35	12085.0
36	12608.5
37	13132.0
38	14838.0
39	16544.0
40	18011.0
41	19478.0
42	21314.5
43	23151.0
44	25274.5
45	27398.0
46	31084.5
47	34771.0
48	38154.0
49	41537.0
50	42760.5
51	43984.0
52	41584.0
53	39184.0
54	35989.0
55	32794.0
56	31096.0
57	29398.0
58	27112.0
59	24826.0
60	22562.0
61	20298.0
62	19193.0
63	18088.0
64	15448.5
65	12809.0
66	11051.5
67	9294.0
68	7824.5
69	6355.0
70	5180.0
71	4005.0
72	3453.0
73	2901.0
74	2448.0
75	1754.0
76	1513.0
77	1036.0
78	559.0
79	414.5
80	270.0
81	232.0
82	194.0
83	117.0
84	40.0
85	25.0
86	10.0
87	16.0
88	22.0
89	14.0
90	6.0
91	3.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	495103.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.82668595169942
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.445681303122	27.415877258103748
2	8.568624814342122	6.311081101514321
3	3.291342240706716	3.6362768117420674
4	1.9157304622391518	2.8220001640394083
5	1.3920196961675382	2.5631736094670985
6	1.138204970444698	2.514979019713812
7	0.963879891522593	2.4847551442182447
8	0.8626090772809616	2.541362687048895
9	0.7220589776867711	2.393193528888827
>10	6.623338140976967	43.63184663596689
>50	0.05355729785735569	1.3558816001934264
>100	0.021313618535069773	1.3391771066775935
>500	5.465030393607634E-4	0.11451634711445006
>1k	0.0010930060787215268	0.8758789853112243
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3330	0.6725873202141777	No Hit
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	1022	0.2064216940717386	RNA PCR Primer, Index 27 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	569	0.11492558114170183	RNA PCR Primer, Index 38 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0197817423849178E-4	0.0	0.0	0.09210204745275226	0.0
2	2.0197817423849178E-4	0.0	0.0	0.5283749038078945	0.0
3	2.0197817423849178E-4	0.0	0.0	0.6962187666000812	0.0
4	2.0197817423849178E-4	0.0	0.0	0.920414540004807	0.0
5	2.0197817423849178E-4	0.0	0.0	1.5425073166593617	0.0
6	2.0197817423849178E-4	0.0	0.0	2.1153174187997243	0.0
7	2.0197817423849178E-4	0.0	0.0	2.577039525108917	0.0
8	2.0197817423849178E-4	0.0	0.0	3.3946471744263316	0.0
9	2.0197817423849178E-4	0.0	0.0	3.748108979343692	0.0
10	2.0197817423849178E-4	0.0	0.0	4.528552644601224	0.0
11	2.0197817423849178E-4	0.0	0.0	5.46653928576478	0.0
12	2.0197817423849178E-4	0.0	0.0	6.240721627620919	0.0
13	2.0197817423849178E-4	0.0	0.0	6.533589980266733	0.0
14	2.0197817423849178E-4	0.0	0.0	6.6660876625671825	0.0
15	2.0197817423849178E-4	0.0	0.0	6.885032003441708	0.0
16	2.0197817423849178E-4	0.0	0.0	7.350995651409908	0.0
17	2.0197817423849178E-4	0.0	0.0	7.910273215876292	0.0
18	2.0197817423849178E-4	0.0	0.0	8.550544028212311	0.0
19	2.0197817423849178E-4	0.0	0.0	8.891483186326886	0.0
20	4.0395634847698356E-4	0.0	0.0	9.214446286934233	0.0
21	4.0395634847698356E-4	0.0	0.0	9.610525486615916	0.0
22	4.0395634847698356E-4	0.0	0.0	10.02660052554721	0.0
23	4.0395634847698356E-4	0.0	0.0	10.465701076341691	0.0
24	4.0395634847698356E-4	0.0	0.0	10.817345077690904	0.0
25	4.0395634847698356E-4	0.0	0.0	11.11647475373811	0.0
26	4.0395634847698356E-4	0.0	0.0	11.407727280990017	0.0
27	4.0395634847698356E-4	0.0	0.0	11.761391064081616	0.0
28	4.0395634847698356E-4	0.0	0.0	12.074457234151277	0.0
29	4.0395634847698356E-4	0.0	0.0	12.439229816825994	0.0
30	4.0395634847698356E-4	0.0	0.0	12.848639576007416	0.0
31	4.0395634847698356E-4	0.0	0.0	13.198263795614245	0.0
32	4.0395634847698356E-4	0.0	0.0	13.556977033061807	0.0
33	4.0395634847698356E-4	0.0	0.0	13.925385222872816	0.0
34	6.059345227154753E-4	0.0	0.0	14.298640888865549	0.0
35	6.059345227154753E-4	0.0	0.0	14.70825262622121	0.0
36	6.059345227154753E-4	0.0	0.0	15.035457268487567	0.0
37	6.059345227154753E-4	0.0	0.0	15.389726986101882	0.0
38	6.059345227154753E-4	0.0	0.0	15.807619828601322	0.0
39	6.059345227154753E-4	0.0	0.0	16.387297188665794	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	40	6.8012014E-9	45.000004	25
TAGACGG	30	2.1629894E-6	45.000004	2
AACTTCG	20	7.029391E-4	45.000004	11
CTATACG	20	7.029391E-4	45.000004	1
TTAGACG	20	7.029391E-4	45.000004	1
ATCACGC	20	7.029391E-4	45.000004	9
GGTGCGT	20	7.029391E-4	45.000004	8
CTCGACG	20	7.029391E-4	45.000004	1
AATACGG	20	7.029391E-4	45.000004	2
GATAGCG	20	7.029391E-4	45.000004	1
TATACGG	45	3.8380676E-10	45.0	2
TCGACGG	35	1.2100463E-7	45.0	2
CGTTTTT	2240	0.0	41.886158	1
GCGATAT	65	0.0	41.53846	9
CTCACGA	45	1.9244908E-8	40.0	24
CGGTCTA	45	1.9244908E-8	40.0	31
ACGGTCT	45	1.9244908E-8	40.0	30
CGTTATT	160	0.0	39.375004	1
TAGTGCG	40	3.4528966E-7	39.375004	1
GACCGAT	230	0.0	39.130436	9
>>END_MODULE