SRR3554345_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3554345_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	357333
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2532	0.7085827505436105	No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	710	0.1986942151998276	TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	667	0.18666062188490848	TruSeq Adapter, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	373	0.10438442573174044	Illumina PCR Primer Index 8 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAACA	20	7.026755E-4	45.0	36
GCAAACT	20	7.026755E-4	45.0	30
CTAACGG	20	7.026755E-4	45.0	2
ACGGAAC	20	7.026755E-4	45.0	35
CACAACG	40	6.7921064E-9	45.0	12
CGCAAAC	20	7.026755E-4	45.0	29
CGATAGT	40	6.7921064E-9	45.0	10
GTACGAG	20	7.026755E-4	45.0	1
CGTTTTT	1475	0.0	42.406776	1
ACGGGCT	60	3.6379788E-12	41.250004	5
AACGAGC	45	1.9219442E-8	40.0	15
ACTACGG	40	3.4493132E-7	39.375	2
CATACGG	40	3.4493132E-7	39.375	2
CGTTATT	115	0.0	39.130436	1
TTAGCGG	70	0.0	38.57143	2
CGGTCTA	35	6.2362087E-6	38.57143	31
TGCACGG	35	6.2362087E-6	38.57143	2
TATGCGG	65	9.094947E-12	38.07692	2
ACGCGGT	30	1.1383479E-4	37.500004	30
TACGGGT	55	2.737579E-9	36.81818	4