##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554345_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	357333
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.978902032557865	33.0	31.0	34.0	30.0	34.0
2	32.05673699322481	33.0	31.0	34.0	30.0	34.0
3	32.01074627868123	33.0	31.0	34.0	30.0	34.0
4	35.66886629558423	37.0	35.0	37.0	33.0	37.0
5	33.865302113154954	37.0	35.0	37.0	32.0	37.0
6	34.59570764524967	37.0	35.0	37.0	31.0	37.0
7	35.648845754520295	37.0	35.0	37.0	33.0	37.0
8	35.54157886341312	37.0	35.0	37.0	33.0	37.0
9	37.42456756023093	39.0	37.0	39.0	34.0	39.0
10	36.92543090058852	39.0	37.0	39.0	33.0	39.0
11	37.09641426904316	39.0	37.0	39.0	34.0	39.0
12	37.02812502623603	39.0	37.0	39.0	33.0	39.0
13	37.1849255456395	39.0	37.0	39.0	34.0	39.0
14	38.19118861118341	40.0	38.0	41.0	33.0	41.0
15	38.31317566527581	40.0	38.0	41.0	34.0	41.0
16	38.16742926066162	40.0	37.0	41.0	33.0	41.0
17	38.185616777627594	40.0	37.0	41.0	34.0	41.0
18	37.96391321260561	39.0	37.0	41.0	33.0	41.0
19	37.79916772310422	39.0	37.0	41.0	33.0	41.0
20	38.049480456604904	40.0	37.0	41.0	34.0	41.0
21	37.88549336333336	40.0	36.0	41.0	34.0	41.0
22	38.089499150652195	40.0	36.0	41.0	34.0	41.0
23	38.11058312554396	40.0	36.0	41.0	34.0	41.0
24	37.94023781738603	40.0	36.0	41.0	34.0	41.0
25	37.563163771608	39.0	35.0	41.0	33.0	41.0
26	37.77549512639471	39.0	36.0	41.0	34.0	41.0
27	37.40758899961661	40.0	36.0	41.0	33.0	41.0
28	37.44202186755771	40.0	35.0	41.0	33.0	41.0
29	37.602432464955655	40.0	35.0	41.0	33.0	41.0
30	37.355189697005315	39.0	35.0	41.0	33.0	41.0
31	37.24447504148791	39.0	35.0	41.0	33.0	41.0
32	37.29964207056163	39.0	35.0	41.0	33.0	41.0
33	37.23929499934235	40.0	35.0	41.0	33.0	41.0
34	37.079681977315275	40.0	35.0	41.0	32.0	41.0
35	36.97401303545992	40.0	35.0	41.0	32.0	41.0
36	36.79589906333868	40.0	35.0	41.0	31.0	41.0
37	36.82218266994652	40.0	35.0	41.0	31.0	41.0
38	36.58564140451624	39.0	35.0	41.0	31.0	41.0
39	36.551186148494544	39.0	35.0	41.0	31.0	41.0
40	36.41377650538853	39.0	35.0	41.0	30.0	41.0
41	36.19219607480977	39.0	35.0	41.0	30.0	41.0
42	36.25203381719572	39.0	35.0	41.0	30.0	41.0
43	36.202861196698876	39.0	35.0	41.0	30.0	41.0
44	36.23565693624714	39.0	35.0	41.0	30.0	41.0
45	36.10786577226285	39.0	35.0	41.0	30.0	41.0
46	36.03660171324787	39.0	35.0	41.0	30.0	41.0
47	35.97696826209726	38.0	35.0	40.0	30.0	41.0
48	35.870896334791354	38.0	35.0	40.0	30.0	41.0
49	35.80692799153731	38.0	35.0	40.0	30.0	41.0
50	35.65011068107339	37.0	35.0	40.0	30.0	41.0
51	34.722650860681775	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	18.0
10	18.0
11	14.0
12	12.0
13	18.0
14	16.0
15	17.0
16	41.0
17	59.0
18	125.0
19	189.0
20	292.0
21	475.0
22	725.0
23	1158.0
24	1733.0
25	2694.0
26	3847.0
27	4561.0
28	4721.0
29	4830.0
30	5435.0
31	6708.0
32	8576.0
33	12498.0
34	22252.0
35	27082.0
36	30404.0
37	46736.0
38	77356.0
39	94699.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.71179264159761	18.335558148841557	23.172502959424403	16.780146250136426
2	35.10534991170701	23.186495509790586	24.44442578771062	17.26372879079178
3	32.01915300294124	23.118211864003605	27.79732070645588	17.06531442659928
4	29.381557258915354	24.996571825160284	26.958327386499427	18.663543529424935
5	23.694425088083104	33.03501215952627	25.283139256659755	17.987423495730873
6	25.522971569936164	33.356001264926554	26.184259500242067	14.936767664895209
7	77.88533384825918	6.314837980259309	11.304021738826249	4.495806432655254
8	79.30082024330247	5.0440345560023845	10.955047532693595	4.700097668001556
9	73.087008476687	7.348047899298414	13.528557396042348	6.036386227972228
10	38.55507327898627	25.139575689902692	20.714851413107663	15.590499618003376
11	29.864020395541413	25.64694556618057	26.14228184914352	18.346752189134506
12	27.569242135487066	22.30020736959643	30.882678062199687	19.24787243271682
13	25.325396758765635	23.043491645048174	31.970738778674235	19.660372817511956
14	21.146381666400806	25.943587633943686	32.35385480770038	20.55617589195512
15	21.009814374826842	24.667746891554938	35.250312733500685	19.072126000117535
16	23.474742047334	23.017745352374398	33.2916355332421	20.215877067049504
17	23.143398454662737	23.057204344407037	30.327733514676787	23.47166368625344
18	23.926701424161774	24.044798549252377	30.765420490131056	21.26307953645479
19	25.354501263527297	24.60757892498034	28.81457911807754	21.223340693414826
20	25.86746815995164	24.555806488625457	29.30347882787204	20.27324652355086
21	25.172038406752247	24.9554337270837	29.824841254516095	20.047686611647958
22	23.247503029387154	24.677541676811266	29.188460063862	22.88649522993958
23	22.795263801552053	25.180993638986603	29.33314303464835	22.690599524812992
24	22.789946632412903	24.287149521594703	30.419244794071638	22.503659051920756
25	23.085469296146734	24.782205953550328	29.28892657549121	22.84339817481173
26	22.163919929029785	26.04965116571937	29.57941192109321	22.207016984157633
27	23.037894624901703	26.4042223919985	28.951706111666148	21.60617687143365
28	22.075487010715495	26.004595153540254	30.26924465414613	21.65067318159812
29	23.391346447151538	25.046665155471224	29.28193030030812	22.28005809706912
30	23.827354316561863	24.929127732395273	29.095269678423207	22.148248272619657
31	24.649836427086218	25.790509132937622	27.72148108347116	21.838173356504996
32	26.17138635390518	25.957300333302552	27.173532811131352	20.697780501660915
33	24.14414565685229	26.26877450445383	26.955808727433517	22.631271111260364
34	24.801515673055665	25.555434286785715	28.041070933834828	21.601979106323792
35	24.564202018845165	25.366534856842215	27.688458664606962	22.380804459705654
36	24.756739511883875	27.327450865159392	26.49881203247391	21.41699759048283
37	24.97418374457439	27.422040505634797	27.10217080426381	20.501604945527003
38	24.57567591014544	25.819053935684643	27.28995083017801	22.315319323991904
39	24.885470975252773	24.640321492837213	27.569242135487066	22.904965396422945
40	24.741907408495717	24.244052466466854	29.61047538290614	21.40356474213129
41	21.609535083521532	26.28304690582734	28.983049424486403	23.124368586164724
42	23.348809094038334	27.052637175967515	27.976705202150377	21.621848527843778
43	23.50888387022749	26.332860385130957	28.491631055625984	21.666624689015567
44	24.369145866740546	25.630434356748466	27.308980698676027	22.69143907783496
45	23.723249741837446	24.43966832058612	27.439671119096193	24.397410818480243
46	23.408697209605606	25.740135951619358	27.595268279168174	23.255898559606866
47	22.794144397522757	26.25981927221947	29.190978722927913	21.75505760732986
48	22.86606610640495	25.780994198688617	28.641071493536842	22.711868201369594
49	23.24554407233589	25.447971499973416	28.69004541981849	22.61643900787221
50	22.02903174349976	24.891627697413895	29.12045626908234	23.958884290004
51	21.711960552202008	24.98873599695522	27.89163049592397	25.4076729549188
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	148.0
1	451.0
2	754.0
3	2768.5
4	4783.0
5	3262.5
6	1742.0
7	1722.5
8	1703.0
9	1683.5
10	1664.0
11	1605.5
12	1547.0
13	1529.0
14	1511.0
15	1405.0
16	1299.0
17	1366.5
18	1434.0
19	1433.0
20	1432.0
21	1514.5
22	1597.0
23	1818.5
24	2040.0
25	2154.5
26	2724.0
27	3179.0
28	3798.0
29	4417.0
30	4918.5
31	5420.0
32	6033.5
33	6647.0
34	7521.5
35	8396.0
36	9478.0
37	10560.0
38	11348.5
39	12137.0
40	13017.0
41	13897.0
42	14976.5
43	16056.0
44	17270.5
45	18485.0
46	20233.5
47	21982.0
48	24511.5
49	27041.0
50	28281.5
51	29522.0
52	29851.5
53	30181.0
54	27477.5
55	24774.0
56	22728.5
57	20683.0
58	19768.5
59	18854.0
60	18002.0
61	17150.0
62	15480.0
63	13810.0
64	11901.0
65	9992.0
66	8425.5
67	6859.0
68	5820.5
69	4782.0
70	3984.5
71	3187.0
72	2678.0
73	2169.0
74	1921.0
75	1196.0
76	719.0
77	566.0
78	413.0
79	320.0
80	227.0
81	180.0
82	133.0
83	90.5
84	48.0
85	30.0
86	12.0
87	8.0
88	4.0
89	2.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	357333.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.33837621568158
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.48269454571968	29.30028259597215
2	8.192459278022731	6.445560904210186
3	3.3534115804865103	3.9575329907760506
4	2.006660637849948	3.1575508443576323
5	1.5674798085499315	3.083105520961087
6	1.2252799317289167	2.892031375432606
7	1.0980440625629355	3.023668930414731
8	0.9758603345559727	3.0711008779778988
9	0.8909026644255713	3.154199776625362
>10	6.140858335830595	38.51273191566727
>50	0.04729118040320216	1.2911441560730568
>100	0.016940124323535102	1.0256960242008326
>500	0.0014116770269612584	0.3823452694435697
>1k	7.058385134806292E-4	0.7030488178875225
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2532	0.7085827505436105	No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	710	0.1986942151998276	TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	667	0.18666062188490848	TruSeq Adapter, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	373	0.10438442573174044	Illumina PCR Primer Index 8 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10774263781962483	0.0
2	0.0	0.0	0.0	0.61931027920735	0.0
3	0.0	0.0	0.0	0.8415119790223685	0.0
4	2.7985100732370085E-4	0.0	0.0	1.0947771406503177	0.0
5	2.7985100732370085E-4	0.0	0.0	1.8268673758091192	0.0
6	2.7985100732370085E-4	0.0	0.0	2.471084394668279	0.0
7	2.7985100732370085E-4	0.0	0.0	3.0081184777224608	0.0
8	2.7985100732370085E-4	0.0	0.0	3.95177607441798	0.0
9	2.7985100732370085E-4	0.0	0.0	4.348604802802988	0.0
10	2.7985100732370085E-4	0.0	0.0	5.182001102612968	0.0
11	2.7985100732370085E-4	0.0	0.0	6.207934895461656	0.0
12	2.7985100732370085E-4	0.0	0.0	7.024819985839539	0.0
13	2.7985100732370085E-4	0.0	0.0	7.398980782631327	0.0
14	2.7985100732370085E-4	0.0	0.0	7.553178687666686	0.0
15	2.7985100732370085E-4	0.0	0.0	7.820436399660821	0.0
16	2.7985100732370085E-4	0.0	0.0	8.35719063170768	0.0
17	2.7985100732370085E-4	0.0	0.0	9.01288154186711	0.0
18	2.7985100732370085E-4	0.0	0.0	9.706352338015241	0.0
19	2.7985100732370085E-4	0.0	0.0	10.073237008616612	0.0
20	2.7985100732370085E-4	0.0	0.0	10.41493508855885	0.0
21	2.7985100732370085E-4	0.0	0.0	10.846185490844674	0.0
22	2.7985100732370085E-4	0.0	0.0	11.287230678386827	0.0
23	5.597020146474017E-4	0.0	0.0	11.73051467398757	0.0
24	5.597020146474017E-4	0.0	0.0	12.085085900266698	0.0
25	5.597020146474017E-4	0.0	0.0	12.423145917113729	0.0
26	5.597020146474017E-4	0.0	0.0	12.729302919125857	0.0
27	5.597020146474017E-4	0.0	0.0	13.079676380295131	0.0
28	5.597020146474017E-4	0.0	0.0	13.400105783680768	0.0
29	5.597020146474017E-4	0.0	0.0	13.747680734776806	0.0
30	5.597020146474017E-4	0.0	0.0	14.155423652447437	0.0
31	5.597020146474017E-4	0.0	0.0	14.50047994447756	0.0
32	5.597020146474017E-4	0.0	0.0	14.867364615078932	0.0
33	5.597020146474017E-4	0.0	0.0	15.241805262878044	0.0
34	5.597020146474017E-4	0.0	0.0	15.626880248955455	0.0
35	5.597020146474017E-4	0.0	0.0	16.02958584849426	0.0
36	5.597020146474017E-4	0.0	0.0	16.414940685578998	0.0
37	5.597020146474017E-4	0.0	0.0	16.802814181729648	0.0
38	5.597020146474017E-4	0.0	0.0	17.176135425499464	0.0
39	5.597020146474017E-4	0.0	0.0	17.676788877601567	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAACA	20	7.026755E-4	45.0	36
GCAAACT	20	7.026755E-4	45.0	30
CTAACGG	20	7.026755E-4	45.0	2
ACGGAAC	20	7.026755E-4	45.0	35
CACAACG	40	6.7921064E-9	45.0	12
CGCAAAC	20	7.026755E-4	45.0	29
CGATAGT	40	6.7921064E-9	45.0	10
GTACGAG	20	7.026755E-4	45.0	1
CGTTTTT	1475	0.0	42.406776	1
ACGGGCT	60	3.6379788E-12	41.250004	5
AACGAGC	45	1.9219442E-8	40.0	15
ACTACGG	40	3.4493132E-7	39.375	2
CATACGG	40	3.4493132E-7	39.375	2
CGTTATT	115	0.0	39.130436	1
TTAGCGG	70	0.0	38.57143	2
CGGTCTA	35	6.2362087E-6	38.57143	31
TGCACGG	35	6.2362087E-6	38.57143	2
TATGCGG	65	9.094947E-12	38.07692	2
ACGCGGT	30	1.1383479E-4	37.500004	30
TACGGGT	55	2.737579E-9	36.81818	4
>>END_MODULE