FastQCFastQC Report
Sat 18 Jun 2016
SRR3554340_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554340_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences733280
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138871.8938195505127646No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTT12360.16855771328823915No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC10100.13773728998472615No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC8870.12096334278856645No Hit
CGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTG8370.1141446650665503No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8350.11387191795766964No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG8000.10909884355225835No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG7570.10323478071132446No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTGCG406.8084773E-945.0000041
AATAGCG207.031613E-445.0000041
TCGGTCA207.031613E-445.00000412
TCGTAAG253.8894188E-545.01
CGTTTTT66450.042.832961
CCTCGTC1200.041.25000436
GTTACGG950.040.2631572
CTCGTCC1250.039.60000237
GTTTGCG356.2451945E-638.571431
TCTACGG356.2451945E-638.571432
GCGATCT356.2451945E-638.571439
TCGACGT356.2451945E-638.5714326
TTCGACG356.2451945E-638.5714325
AGTGTCG356.2451945E-638.571431
GCATACG356.2451945E-638.571431
TTACGGG1900.037.8947373
TCACGAC601.546141E-1037.50000425
TCTAGCG301.13951864E-437.5000041
TGCGACG301.13951864E-437.5000041
TATCGAG301.13951864E-437.5000041