##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554340_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	733280
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.97308940650229	33.0	31.0	34.0	30.0	34.0
2	32.097490726598295	33.0	31.0	34.0	30.0	34.0
3	32.06971961597207	33.0	31.0	34.0	30.0	34.0
4	35.70585451669212	37.0	35.0	37.0	33.0	37.0
5	35.522261619026835	37.0	35.0	37.0	33.0	37.0
6	35.641928049312675	37.0	35.0	37.0	33.0	37.0
7	35.95607135064368	37.0	35.0	37.0	35.0	37.0
8	35.88498663539166	37.0	35.0	37.0	35.0	37.0
9	37.74111117172158	39.0	38.0	39.0	35.0	39.0
10	37.31828360244381	39.0	37.0	39.0	34.0	39.0
11	37.26608526074624	39.0	37.0	39.0	34.0	39.0
12	37.127291075714595	39.0	37.0	39.0	34.0	39.0
13	37.118349061749946	39.0	37.0	39.0	33.0	39.0
14	38.17006873227144	40.0	38.0	41.0	33.0	41.0
15	38.333302421994325	40.0	38.0	41.0	33.0	41.0
16	38.273019855989524	40.0	37.0	41.0	33.0	41.0
17	38.26996781584115	40.0	37.0	41.0	34.0	41.0
18	38.11467243072224	40.0	37.0	41.0	34.0	41.0
19	37.93074814531966	40.0	37.0	41.0	34.0	41.0
20	37.85540175649138	40.0	35.0	41.0	34.0	41.0
21	37.74639019201396	40.0	35.0	41.0	33.0	41.0
22	37.817567641283006	40.0	35.0	41.0	34.0	41.0
23	37.82377945668776	40.0	35.0	41.0	34.0	41.0
24	37.74611062622736	40.0	35.0	41.0	34.0	41.0
25	37.52317123063496	39.0	35.0	41.0	33.0	41.0
26	37.57411084442505	39.0	35.0	41.0	33.0	41.0
27	37.58473979925813	39.0	35.0	41.0	33.0	41.0
28	37.47765246563387	39.0	35.0	41.0	33.0	41.0
29	37.39710888064587	39.0	35.0	41.0	33.0	41.0
30	37.14188713724634	39.0	35.0	41.0	33.0	41.0
31	36.97028011128082	39.0	35.0	41.0	32.0	41.0
32	36.72735380754964	39.0	35.0	41.0	31.0	41.0
33	36.50792875845516	39.0	35.0	41.0	31.0	41.0
34	36.22504636700851	39.0	35.0	41.0	30.0	41.0
35	36.02273210778966	39.0	35.0	41.0	29.0	41.0
36	35.85630182195069	39.0	35.0	41.0	27.0	41.0
37	35.83091588479162	39.0	35.0	41.0	27.0	41.0
38	35.72998990835697	39.0	35.0	41.0	27.0	41.0
39	35.65969070477853	39.0	35.0	41.0	26.0	41.0
40	35.56895046912503	39.0	35.0	41.0	25.0	41.0
41	35.432735108007854	39.0	35.0	41.0	24.0	41.0
42	35.469943268601355	39.0	35.0	41.0	25.0	41.0
43	35.39491463015492	39.0	35.0	41.0	25.0	41.0
44	35.32478043857735	39.0	35.0	41.0	24.0	41.0
45	35.27878845734235	38.0	35.0	41.0	24.0	41.0
46	35.133988380973165	38.0	35.0	41.0	24.0	41.0
47	35.081821405193104	38.0	35.0	41.0	23.0	41.0
48	34.98318650447305	38.0	35.0	40.0	23.0	41.0
49	34.97540775692778	38.0	35.0	40.0	24.0	41.0
50	34.90906202269256	38.0	34.0	40.0	24.0	41.0
51	34.09742117608553	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	6.0
10	14.0
11	24.0
12	16.0
13	29.0
14	38.0
15	50.0
16	104.0
17	165.0
18	319.0
19	525.0
20	889.0
21	1264.0
22	1952.0
23	3180.0
24	5053.0
25	8602.0
26	12157.0
27	13963.0
28	13520.0
29	12887.0
30	12857.0
31	14837.0
32	17854.0
33	25105.0
34	48267.0
35	55483.0
36	51203.0
37	71251.0
38	125967.0
39	235482.0
40	210.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.47316168448614	19.528283875190926	22.523183504254852	19.475370936068078
2	39.56619572332533	22.01382827842025	22.30184922539821	16.118126772856208
3	30.777874754527602	21.696350643683175	31.916048439886534	15.609726161902685
4	27.33594261400829	23.206960506218635	31.49888173685359	17.958215142919485
5	24.28294785075278	26.603343879554874	30.876472834387958	18.237235435304385
6	25.07214161029893	30.758509709797078	30.137737289984727	14.031611389919266
7	73.64171939777438	5.711597207069604	16.07776020074187	4.56892319441414
8	75.21697032511454	5.017592188522801	15.49012655465852	4.275310931704124
9	69.40118372245254	6.668394065022911	17.54391228452978	6.386509927994763
10	39.7710288020947	22.00591861226271	24.37145428758455	13.85159829805804
11	30.026456469561424	23.018083133318786	29.778256600480034	17.177203796639755
12	27.002918394065023	21.099307222343445	33.7486362644556	18.149138119135937
13	24.59892537639101	20.561313550076367	35.30479489417412	19.5349661793585
14	20.022638010037095	22.751336460833514	36.82413266419376	20.40189286493563
15	19.966861226271	23.07754200305477	38.087360898974474	18.86823587169976
16	24.279674885446216	23.358880645865156	34.50400938250054	17.857435086188087
17	23.97733471525202	23.45011455378573	33.09308858826097	19.479462142701287
18	23.83482435086188	23.379882173248966	34.13020946977962	18.655084006109533
19	23.418748636264457	25.545494217761295	32.117472179794895	18.918284966179357
20	24.212988217324895	25.905247654374865	32.54991272092516	17.331851407375083
21	23.839461051712853	25.564995636046255	33.280465852061965	17.31507746017892
22	22.838070041457563	22.48404429413048	34.92554003927558	19.752345625136375
23	22.046694305040365	23.59889810168012	34.82653283875191	19.527874754527602
24	22.176521928867555	23.57680558586079	34.176985598952655	20.069686886319005
25	23.299967270346933	24.6032893301331	31.76958324241763	20.327160157102334
26	21.21154265764783	24.547785293475886	32.71615208378791	21.52451996508837
27	21.244681431376826	23.772638010037095	34.17371263364608	20.808967924939996
28	20.48589897447087	25.109507964215577	33.10618044948724	21.298412611826315
29	22.805067641283003	25.06750490944796	32.25166375736418	19.875763691904865
30	24.254582151429194	23.3448341697578	32.957669648701724	19.44291403011128
31	24.317586733580622	23.537257255073097	32.21347916212088	19.9316768492254
32	24.786575387300893	24.380182195068734	31.440922976216452	19.39231944141392
33	25.226107353262055	24.580924067204887	30.21478834824351	19.97818023128955
34	22.843524983635174	24.78493890464761	32.08760637137246	20.283929740344753
35	22.84175212742745	26.156584115208382	30.961706305913157	20.039957451451016
36	23.32287802749291	28.135500763691905	28.962606371372463	19.579014837442724
37	22.987535457124157	27.815704778529348	29.34104298494436	19.855716779402137
38	22.502045603316606	27.328578442068512	29.61910866244818	20.5502672921667
39	23.10018001309186	25.749236308095135	29.08834278856644	22.062240890246564
40	23.8409611608117	24.094343224961815	31.198178049312673	20.86651756491381
41	20.82901483744272	25.745554222125243	30.842106698668992	22.583324241763037
42	21.426058258782458	25.97411629936723	30.75073641719398	21.849089024656337
43	21.751172812568186	25.305476761946323	30.751691032075062	22.19165939341043
44	22.674830896792493	24.30994981453197	30.331114990181103	22.684104298494436
45	22.405084006109536	24.13948287148156	29.63820096006982	23.81723216233908
46	21.99364499236308	24.972043421339734	29.980634955269476	23.053676631027713
47	21.080078551167357	25.87401811040803	31.58329696705215	21.462606371372463
48	21.015028365699322	25.26974689068296	31.87036329914903	21.844861444468687
49	21.91850316386646	24.337360898974474	31.33782456905957	22.406311368099498
50	20.417030329478507	24.62838206415012	31.71926140082915	23.23532620554222
51	20.53253873008946	23.8775092734017	31.306458651538293	24.283493344970545
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	307.0
1	814.5
2	1322.0
3	11742.0
4	22162.0
5	14349.5
6	6537.0
7	6287.5
8	6038.0
9	5904.5
10	5771.0
11	5592.0
12	5413.0
13	5243.0
14	5073.0
15	4771.0
16	4469.0
17	4254.0
18	4039.0
19	3885.0
20	3731.0
21	4009.0
22	4287.0
23	4524.5
24	4762.0
25	5051.5
26	6187.0
27	7033.0
28	7944.0
29	8855.0
30	9989.5
31	11124.0
32	12763.5
33	14403.0
34	16817.0
35	19231.0
36	20252.5
37	21274.0
38	22917.0
39	24560.0
40	26861.0
41	29162.0
42	31274.0
43	33386.0
44	35596.5
45	37807.0
46	43754.5
47	49702.0
48	53809.0
49	57916.0
50	58585.0
51	59254.0
52	54593.0
53	49932.0
54	46496.5
55	43061.0
56	41108.5
57	39156.0
58	37344.0
59	35532.0
60	33531.0
61	31530.0
62	28960.5
63	26391.0
64	23402.0
65	20413.0
66	16767.5
67	13122.0
68	10741.0
69	8360.0
70	6824.0
71	5288.0
72	4314.5
73	3341.0
74	2698.5
75	1634.0
76	1212.0
77	873.5
78	535.0
79	367.0
80	199.0
81	169.5
82	140.0
83	88.5
84	37.0
85	22.5
86	8.0
87	6.0
88	4.0
89	3.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	733280.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.02173827828171
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.23461169303312	25.659945387614652
2	8.05834434420008	5.80551141846094
3	3.7482796914392438	4.050586501164689
4	2.6216858881302394	3.777507316403682
5	2.0072948038512104	3.615312403584156
6	1.6951862323683748	3.6638132877192024
7	1.4218425897560327	3.5852069148774266
8	1.2058649601155598	3.4749881593786647
9	1.1016518618378874	3.571507353681554
>10	6.826621538643177	36.62960547428537
>50	0.04747367107612952	1.1896713206644938
>100	0.026205464181435524	1.7784873679216056
>500	0.003797893359628229	0.9907542706529281
>1k	7.595786719256459E-4	0.30726789448860486
>5k	0.0	0.0
>10k+	3.7978933596282294E-4	1.8998349291020729
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13887	1.8938195505127646	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTT	1236	0.16855771328823915	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC	1010	0.13773728998472615	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC	887	0.12096334278856645	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTG	837	0.1141446650665503	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	835	0.11387191795766964	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	800	0.10909884355225835	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	757	0.10323478071132446	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3637355444032293E-4	0.0	0.0	0.06436831769583243	0.0
2	1.3637355444032293E-4	0.0	0.0	0.28133864281038623	0.0
3	1.3637355444032293E-4	0.0	0.0	0.5165830242199433	0.0
4	1.3637355444032293E-4	0.0	0.0	0.8093770456033166	0.0
5	1.3637355444032293E-4	0.0	0.0	1.616981234998909	0.0
6	1.3637355444032293E-4	0.0	0.0	2.5062731835042547	0.0
7	1.3637355444032293E-4	0.0	0.0	3.2004145756054987	0.0
8	1.3637355444032293E-4	0.0	0.0	4.05820423303513	0.0
9	1.3637355444032293E-4	0.0	0.0	4.716206633209688	0.0
10	1.3637355444032293E-4	0.0	0.0	5.630864062840934	0.0
11	1.3637355444032293E-4	0.0	0.0	6.567068514073751	0.0
12	1.3637355444032293E-4	0.0	0.0	7.263937377263801	0.0
13	1.3637355444032293E-4	0.0	0.0	7.598734453414794	1.3637355444032293E-4
14	1.3637355444032293E-4	0.0	0.0	7.830705869517783	1.3637355444032293E-4
15	1.3637355444032293E-4	0.0	0.0	8.025992799476326	1.3637355444032293E-4
16	1.3637355444032293E-4	0.0	0.0	8.316877591097535	1.3637355444032293E-4
17	1.3637355444032293E-4	0.0	0.0	8.650310931704125	1.3637355444032293E-4
18	1.3637355444032293E-4	0.0	0.0	9.026020074187214	1.3637355444032293E-4
19	1.3637355444032293E-4	0.0	0.0	9.292493999563604	1.3637355444032293E-4
20	1.3637355444032293E-4	0.0	0.0	9.570696050621864	1.3637355444032293E-4
21	1.3637355444032293E-4	0.0	0.0	9.889401047348898	1.3637355444032293E-4
22	1.3637355444032293E-4	0.0	0.0	10.233335151647392	1.3637355444032293E-4
23	1.3637355444032293E-4	0.0	0.0	10.556676849225399	1.3637355444032293E-4
24	1.3637355444032293E-4	0.0	0.0	10.83187868208597	1.3637355444032293E-4
25	1.3637355444032293E-4	0.0	0.0	11.095352389264674	1.3637355444032293E-4
26	1.3637355444032293E-4	0.0	0.0	11.352962033602443	1.3637355444032293E-4
27	1.3637355444032293E-4	0.0	0.0	11.625163648265328	1.3637355444032293E-4
28	1.3637355444032293E-4	0.0	0.0	11.898865372027057	1.3637355444032293E-4
29	1.3637355444032293E-4	0.0	0.0	12.181295003272965	1.3637355444032293E-4
30	2.7274710888064587E-4	0.0	0.0	12.555776783766092	1.3637355444032293E-4
31	2.7274710888064587E-4	0.0	0.0	12.864662884573423	1.3637355444032293E-4
32	2.7274710888064587E-4	0.0	0.0	13.180640410211652	1.3637355444032293E-4
33	2.7274710888064587E-4	0.0	0.0	13.47493454069387	1.3637355444032293E-4
34	2.7274710888064587E-4	0.0	0.0	13.786820859698887	1.3637355444032293E-4
35	2.7274710888064587E-4	0.0	0.0	14.120936068077679	1.3637355444032293E-4
36	2.7274710888064587E-4	0.0	0.0	14.462006327732926	1.3637355444032293E-4
37	2.7274710888064587E-4	0.0	0.0	14.800621863408248	1.3637355444032293E-4
38	2.7274710888064587E-4	0.0	0.0	15.213560986253546	1.3637355444032293E-4
39	2.7274710888064587E-4	0.0	0.0	15.82765110189832	1.3637355444032293E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATTGCG	40	6.8084773E-9	45.000004	1
AATAGCG	20	7.031613E-4	45.000004	1
TCGGTCA	20	7.031613E-4	45.000004	12
TCGTAAG	25	3.8894188E-5	45.0	1
CGTTTTT	6645	0.0	42.83296	1
CCTCGTC	120	0.0	41.250004	36
GTTACGG	95	0.0	40.263157	2
CTCGTCC	125	0.0	39.600002	37
GTTTGCG	35	6.2451945E-6	38.57143	1
TCTACGG	35	6.2451945E-6	38.57143	2
GCGATCT	35	6.2451945E-6	38.57143	9
TCGACGT	35	6.2451945E-6	38.57143	26
TTCGACG	35	6.2451945E-6	38.57143	25
AGTGTCG	35	6.2451945E-6	38.57143	1
GCATACG	35	6.2451945E-6	38.57143	1
TTACGGG	190	0.0	37.894737	3
TCACGAC	60	1.546141E-10	37.500004	25
TCTAGCG	30	1.13951864E-4	37.500004	1
TGCGACG	30	1.13951864E-4	37.500004	1
TATCGAG	30	1.13951864E-4	37.500004	1
>>END_MODULE