FastQCFastQC Report
Sat 18 Jun 2016
SRR3554339_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554339_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474414
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27150.572284966295261No Hit
GCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGC10490.2211148912131598No Hit
CCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGC7690.16209471052709237No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTC7570.1595652742119752No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCG6660.14038371548900327No Hit
CTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGCT6590.1389082109718516Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGC6180.13026597022853456No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT4980.1049716070773628No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC4890.10307452984102491No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCC253.887331E-545.031
GTTAGCG351.209919E-745.01
TTGCGAC207.029094E-445.022
CGATCAT207.029094E-445.010
CGAACGG302.1627966E-644.9999962
CGTTTTT14650.041.4675751
TAGACGG403.4524965E-739.3752
ACGGGTA403.4524965E-739.3755
AGTCCGA301.13891176E-437.49999633
CGAACGT301.13891176E-437.49999637
CGTTAGG301.13891176E-437.4999962
ATGAACG301.13891176E-437.4999961
TTAGGGA3650.036.98634
GTCGAGG552.741217E-936.818182
TAGGGAC3250.036.6923075
AGGGCGA2850.036.315796
TTAGGCG250.002105864536.01
ACGTTGG250.002105864536.02
TAACGCG250.002105864536.01
AACGGGC250.002105864536.04