FastQCFastQC Report
Sat 18 Jun 2016
SRR3554337_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554337_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences556348
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50270.9035711461171785No Hit
GCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC7440.13372924859979723No Hit
CTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGCT5590.10047668006355735No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGG453.8380676E-1045.0000042
TCGACGG207.0301443E-445.02
TCGTTTA253.8882026E-544.99999638
GAGTACG302.163475E-644.9999961
TAACGCG302.163475E-644.9999961
CGTTTAT253.8882026E-544.99999639
CGTTTTT21700.042.2004621
CGCACGG451.9252184E-840.0000042
CGAGGGT800.039.3754
TGTAGCG403.4539335E-739.3751
GCGATAT356.242479E-638.5714269
GCTACGA1000.038.24999610
CCCTACG301.13916474E-437.49999632
TAACGCC301.13916474E-437.49999612
TCGGCAT301.13916474E-437.49999632
CGCCAGT301.13916474E-437.49999626
CGTAAGG1150.037.1739122
CCTATGC850.037.05882335
ACGGGTA800.036.56255
GCGCGAC1850.036.486499
Produced by FastQC (version 0.11.5)