##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554337_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	556348
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.967227346912363	33.0	31.0	34.0	30.0	34.0
2	31.995258363470345	33.0	31.0	34.0	30.0	34.0
3	32.042352987698344	33.0	31.0	34.0	30.0	34.0
4	35.68797227634502	37.0	35.0	37.0	33.0	37.0
5	35.49698390216196	37.0	35.0	37.0	33.0	37.0
6	35.62302371896727	37.0	35.0	37.0	33.0	37.0
7	35.94157613579989	37.0	35.0	37.0	35.0	37.0
8	35.848479728515244	37.0	35.0	37.0	35.0	37.0
9	37.692029089706445	39.0	38.0	39.0	35.0	39.0
10	37.27981587064212	39.0	37.0	39.0	34.0	39.0
11	37.215304090245674	39.0	37.0	39.0	34.0	39.0
12	37.034859476442804	39.0	37.0	39.0	33.0	39.0
13	36.98418435942971	39.0	37.0	39.0	33.0	39.0
14	38.00215692336451	40.0	37.0	41.0	33.0	41.0
15	38.17871907511126	40.0	37.0	41.0	33.0	41.0
16	38.14535326809838	40.0	37.0	41.0	33.0	41.0
17	38.13649730025092	40.0	37.0	41.0	33.0	41.0
18	38.06423677266747	40.0	37.0	41.0	34.0	41.0
19	37.966774393005814	40.0	37.0	41.0	34.0	41.0
20	37.98634847253877	40.0	36.0	41.0	34.0	41.0
21	37.86944322618217	40.0	35.0	41.0	33.0	41.0
22	37.95157527303055	40.0	35.0	41.0	34.0	41.0
23	37.957663189226885	40.0	35.0	41.0	34.0	41.0
24	37.88954215706716	40.0	35.0	41.0	34.0	41.0
25	37.64513218345352	40.0	35.0	41.0	33.0	41.0
26	37.71023172546679	40.0	35.0	41.0	33.0	41.0
27	37.71615787241079	40.0	35.0	41.0	34.0	41.0
28	37.621537239281885	40.0	35.0	41.0	33.0	41.0
29	37.58427638815993	40.0	35.0	41.0	33.0	41.0
30	37.39105919316687	40.0	35.0	41.0	33.0	41.0
31	37.31019793366742	40.0	35.0	41.0	33.0	41.0
32	37.203554250217486	40.0	35.0	41.0	33.0	41.0
33	37.12125504180837	40.0	35.0	41.0	32.0	41.0
34	36.94727041348221	40.0	35.0	41.0	32.0	41.0
35	36.838876027234754	40.0	35.0	41.0	31.0	41.0
36	36.70717248916146	39.0	35.0	41.0	31.0	41.0
37	36.701330821715906	39.0	35.0	41.0	31.0	41.0
38	36.602414316219345	39.0	35.0	41.0	31.0	41.0
39	36.537679653741904	39.0	35.0	41.0	31.0	41.0
40	36.439906317628534	39.0	35.0	41.0	31.0	41.0
41	36.30700029477953	39.0	35.0	41.0	30.0	41.0
42	36.34064110952138	39.0	35.0	41.0	31.0	41.0
43	36.257917706183896	39.0	35.0	41.0	30.0	41.0
44	36.21344733871606	39.0	35.0	41.0	30.0	41.0
45	36.163750026961544	39.0	35.0	41.0	30.0	41.0
46	36.01687612789117	38.0	35.0	41.0	30.0	41.0
47	35.9719438193361	38.0	35.0	41.0	30.0	41.0
48	35.88357826396428	38.0	35.0	41.0	30.0	41.0
49	35.85379654460877	38.0	35.0	41.0	30.0	41.0
50	35.76340527871045	38.0	35.0	40.0	30.0	41.0
51	34.95884230733282	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	8.0
10	11.0
11	21.0
12	16.0
13	16.0
14	31.0
15	33.0
16	78.0
17	89.0
18	195.0
19	342.0
20	532.0
21	814.0
22	1187.0
23	1889.0
24	2962.0
25	4360.0
26	5869.0
27	6727.0
28	7156.0
29	7477.0
30	8668.0
31	10561.0
32	13526.0
33	19374.0
34	37753.0
35	46741.0
36	39730.0
37	57421.0
38	98093.0
39	184503.0
40	160.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.15553574381502	20.5928663354591	23.687691876307635	14.563906044418243
2	35.08128006211939	23.05499435605053	23.992177557931367	17.87154802389871
3	32.987266962404824	22.724805337666353	27.273936457037685	17.01399124289114
4	28.724287676058868	24.50426711338946	27.388972369811697	19.38247284073997
5	25.61292572274907	28.85406256515706	26.515777894411414	19.01723381768246
6	26.412425316528505	32.91752644028558	26.09949168506043	14.570556558125489
7	77.2437395299345	6.5920970327924255	11.265790476464371	4.898372960808702
8	78.63998792122916	5.8610797558362755	10.891025041880262	4.607907281054304
9	72.19869578033892	7.985829013495151	13.175566372126799	6.639908834039127
10	42.65172158433211	23.675109823347977	19.736567759747498	13.936600832572418
11	33.36652598733167	24.26664605606563	24.837511773206696	17.529316183396006
12	30.557852279508506	22.107026537347128	28.4553912299496	18.87972995319476
13	26.86681717198588	22.298992716788774	30.308547887293564	20.525642223931783
14	21.67186724855666	25.20508746324243	32.37865508638478	20.744390201816127
15	21.88216727659666	23.579486220854573	34.546183324106494	19.992163178442272
16	25.864566781942237	23.495545953252282	31.811743728745316	18.828143536060164
17	25.433541596267084	23.44395953611768	29.903945012833695	21.21855385478154
18	26.061745526181458	23.86186343799205	30.05043605800686	20.025954977819637
19	26.639261757029775	25.826461135835842	28.11945041592672	19.41482669120766
20	27.955883727451162	25.73317420032066	28.16798119162826	18.14296088059991
21	25.893146016522035	26.18451041434498	29.294614162358812	18.627729406774176
22	24.305470676626857	23.772351118364764	30.442456879507073	21.479721325501306
23	24.355978632079204	25.0668646242999	29.939893735575573	20.637263008045323
24	24.081510133944942	23.91003472646617	30.2289214664203	21.77953367316859
25	24.596295843608676	24.82762587445268	28.236643252065253	22.33943502987339
26	22.266459122707367	26.03604218942101	28.866824361730426	22.830674326141192
27	23.218381300912377	24.940684607475898	29.761408327162137	22.07952576444959
28	20.836598675649054	26.938175386628515	29.705867550525927	22.519358387196505
29	23.13983334172137	26.616793805316096	28.80229640440875	21.441076448553783
30	23.982830890018477	25.1414582239893	28.953640527152068	21.922070358840152
31	24.621819436755413	25.66972470468124	27.925866543961693	21.78258931460165
32	25.566731614025755	25.658580600631257	28.074155025271953	20.700532760071034
33	25.1872928454852	26.455204296591344	26.760588696283623	21.596914161639837
34	22.390122728939442	26.546514052355718	29.452608798809376	21.61075441989546
35	22.800117911810595	27.136792079777404	28.112080927764637	21.951009080647367
36	22.54811736539001	30.14893555831961	25.626226750163568	21.676720326126812
37	23.369725423655694	28.332626341786078	26.333697613723782	21.963950620834442
38	22.76794380495661	28.233407867018485	25.498788528043598	23.49985979998131
39	23.2838079763026	26.162401949858722	26.61535585640642	23.938434217432256
40	23.430838252316896	24.946975633955727	28.21399555673787	23.40819055698951
41	20.92179714854731	26.24562324300618	27.401015191930227	25.431564416516284
42	22.416365296541013	26.11459014861202	27.459971097226916	24.00907345762005
43	22.180721418967984	25.71466060810859	28.178945552064533	23.925672420858888
44	22.570046086262554	25.28830875638989	27.598733167010575	24.542911990336982
45	22.25189988999691	24.82439048940591	27.620661887883124	25.303047732714056
46	21.777736237031498	26.293435044252877	27.13535413086773	24.793474587847893
47	21.13407435633812	26.584979185689534	28.761674347710425	23.51927211026192
48	21.535441845751222	25.71376189004005	28.96047078447303	23.790325479735706
49	22.729119184395376	25.221084644862568	28.449819177924606	23.599976992817446
50	20.394788873151338	25.223061824613374	29.007563611264892	25.3745856909704
51	20.46938247284074	25.527906993464523	27.9699037293205	26.032806804374243
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	399.0
1	853.5
2	1308.0
3	4732.0
4	8156.0
5	5349.5
6	2543.0
7	2424.5
8	2306.0
9	2233.0
10	2160.0
11	2122.0
12	2084.0
13	1990.0
14	1896.0
15	1996.0
16	2096.0
17	2007.0
18	1918.0
19	1983.5
20	2049.0
21	2237.5
22	2426.0
23	2535.0
24	2644.0
25	3023.5
26	4270.5
27	5138.0
28	5829.5
29	6521.0
30	7320.0
31	8119.0
32	9623.0
33	11127.0
34	12395.5
35	13664.0
36	14615.5
37	15567.0
38	16912.0
39	18257.0
40	20684.5
41	23112.0
42	24520.0
43	25928.0
44	28122.0
45	30316.0
46	33447.0
47	36578.0
48	40518.0
49	44458.0
50	44800.5
51	45143.0
52	41869.5
53	38596.0
54	36132.5
55	33669.0
56	32416.5
57	31164.0
58	30696.5
59	30229.0
60	29116.5
61	28004.0
62	25883.5
63	23763.0
64	20220.0
65	16677.0
66	13942.0
67	11207.0
68	9545.0
69	7883.0
70	6855.5
71	5828.0
72	4938.5
73	4049.0
74	3412.0
75	2134.0
76	1493.0
77	1215.0
78	937.0
79	670.0
80	403.0
81	308.0
82	213.0
83	146.5
84	80.0
85	50.5
86	21.0
87	20.0
88	19.0
89	12.5
90	6.0
91	10.5
92	15.0
93	8.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	556348.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.04174359672994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.38848189906638	24.301883965678144
2	8.295563481725756	5.6479089007060885
3	3.614929942918231	3.6917555471089205
4	2.257840195441091	3.07443268062385
5	1.7465764641932484	2.9728254083074868
6	1.4353655660890725	2.93174079410276
7	1.2670942143610269	3.0193867450724627
8	1.0809478178473406	2.943787876528312
9	0.9580789004919302	2.9353208648384905
>10	7.877647123800556	44.396295400992436
>50	0.04881948119187164	1.1309953843197893
>100	0.026532326734701767	1.7153700957510405
>500	0.0015919396040821056	0.33021235625873024
>1k	0.0	0.0
>5k	5.306465346940352E-4	0.9080839797115081
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5027	0.9035711461171785	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC	744	0.13372924859979723	No Hit
CTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGCT	559	0.10047668006355735	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.594872274188098E-4	0.0	0.0	0.10335257788290782	0.0
2	3.594872274188098E-4	0.0	0.0	0.4078382595066397	0.0
3	5.392308411282147E-4	0.0	0.0	0.6053764909732757	0.0
4	5.392308411282147E-4	0.0	0.0	0.8381444707269551	0.0
5	5.392308411282147E-4	0.0	0.0	1.48000891528324	0.0
6	5.392308411282147E-4	0.0	0.0	2.0138474480001727	0.0
7	5.392308411282147E-4	0.0	0.0	2.50993982183813	0.0
8	5.392308411282147E-4	0.0	0.0	3.345388138359444	0.0
9	5.392308411282147E-4	0.0	0.0	3.8098456361845465	0.0
10	5.392308411282147E-4	0.0	0.0	4.601975741801894	0.0
11	5.392308411282147E-4	0.0	0.0	5.504827913464235	0.0
12	5.392308411282147E-4	0.0	0.0	6.243574165809889	0.0
13	5.392308411282147E-4	0.0	0.0	6.564955747122305	0.0
14	5.392308411282147E-4	0.0	0.0	6.725287050551094	0.0
15	5.392308411282147E-4	0.0	0.0	6.945832464572534	1.797436137094049E-4
16	7.189744548376197E-4	0.0	0.0	7.347020210371926	1.797436137094049E-4
17	7.189744548376197E-4	0.0	0.0	7.820464888882498	1.797436137094049E-4
18	7.189744548376197E-4	0.0	0.0	8.335969573001071	1.797436137094049E-4
19	7.189744548376197E-4	0.0	0.0	8.67784192627636	1.797436137094049E-4
20	7.189744548376197E-4	0.0	0.0	9.050809924723374	1.797436137094049E-4
21	7.189744548376197E-4	0.0	0.0	9.473027673326767	1.797436137094049E-4
22	7.189744548376197E-4	0.0	0.0	9.931733375513168	1.797436137094049E-4
23	7.189744548376197E-4	0.0	0.0	10.36293830480203	1.797436137094049E-4
24	7.189744548376197E-4	0.0	0.0	10.717033223809558	1.797436137094049E-4
25	7.189744548376197E-4	0.0	0.0	11.04254890823729	1.797436137094049E-4
26	7.189744548376197E-4	0.0	0.0	11.352247154658595	1.797436137094049E-4
27	7.189744548376197E-4	0.0	0.0	11.691423353728242	1.797436137094049E-4
28	7.189744548376197E-4	0.0	0.0	12.01819724345194	1.797436137094049E-4
29	7.189744548376197E-4	0.0	0.0	12.364383443456253	1.797436137094049E-4
30	8.987180685470246E-4	0.0	0.0	12.782287345330621	1.797436137094049E-4
31	8.987180685470246E-4	0.0	0.0	13.145189701409908	0.0012582052959658344
32	8.987180685470246E-4	0.0	0.0	13.50701359580694	0.0012582052959658344
33	0.0010784616822564295	0.0	0.0	13.86955646465881	0.0012582052959658344
34	0.0010784616822564295	0.0	0.0	14.22023625500586	0.0012582052959658344
35	0.0010784616822564295	0.0	0.0	14.57666784099161	0.0012582052959658344
36	0.0010784616822564295	0.0	0.0	14.935076606728163	0.0012582052959658344
37	0.0010784616822564295	0.0	0.0	15.318469734770323	0.0012582052959658344
38	0.0010784616822564295	0.0	0.0	15.777534924184144	0.0012582052959658344
39	0.0010784616822564295	0.0	0.0	16.406278084939643	0.0012582052959658344
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATACGG	45	3.8380676E-10	45.000004	2
TCGACGG	20	7.0301443E-4	45.0	2
TCGTTTA	25	3.8882026E-5	44.999996	38
GAGTACG	30	2.163475E-6	44.999996	1
TAACGCG	30	2.163475E-6	44.999996	1
CGTTTAT	25	3.8882026E-5	44.999996	39
CGTTTTT	2170	0.0	42.200462	1
CGCACGG	45	1.9252184E-8	40.000004	2
CGAGGGT	80	0.0	39.375	4
TGTAGCG	40	3.4539335E-7	39.375	1
GCGATAT	35	6.242479E-6	38.571426	9
GCTACGA	100	0.0	38.249996	10
CCCTACG	30	1.13916474E-4	37.499996	32
TAACGCC	30	1.13916474E-4	37.499996	12
TCGGCAT	30	1.13916474E-4	37.499996	32
CGCCAGT	30	1.13916474E-4	37.499996	26
CGTAAGG	115	0.0	37.173912	2
CCTATGC	85	0.0	37.058823	35
ACGGGTA	80	0.0	36.5625	5
GCGCGAC	185	0.0	36.48649	9
>>END_MODULE