##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554335_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	582851
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.949549713391587	33.0	31.0	34.0	30.0	34.0
2	32.03875604571323	33.0	31.0	34.0	30.0	34.0
3	32.069362495732186	33.0	31.0	34.0	30.0	34.0
4	35.71560313013103	37.0	35.0	37.0	33.0	37.0
5	35.47445058857238	37.0	35.0	37.0	33.0	37.0
6	35.606134329356905	37.0	35.0	37.0	33.0	37.0
7	35.91224515356412	37.0	35.0	37.0	35.0	37.0
8	35.770478218275336	37.0	35.0	37.0	35.0	37.0
9	37.586412307776776	39.0	37.0	39.0	35.0	39.0
10	37.17053758164608	39.0	37.0	39.0	34.0	39.0
11	37.1536499036632	39.0	37.0	39.0	34.0	39.0
12	37.056019462950225	39.0	37.0	39.0	33.0	39.0
13	37.02217547881019	39.0	37.0	39.0	33.0	39.0
14	38.162334799116756	40.0	37.0	41.0	33.0	41.0
15	38.28719175226602	40.0	38.0	41.0	33.0	41.0
16	38.276592130750394	40.0	37.0	41.0	34.0	41.0
17	38.22985462836986	40.0	37.0	41.0	34.0	41.0
18	38.19834228645057	40.0	37.0	41.0	34.0	41.0
19	38.10182019075201	40.0	37.0	41.0	34.0	41.0
20	38.04501836661514	40.0	37.0	41.0	34.0	41.0
21	38.00553314655032	40.0	36.0	41.0	33.0	41.0
22	38.04231270084464	40.0	37.0	41.0	34.0	41.0
23	37.978586293924174	40.0	36.0	41.0	34.0	41.0
24	37.96651974518359	40.0	36.0	41.0	33.0	41.0
25	37.80673448274087	40.0	36.0	41.0	33.0	41.0
26	37.80550260701277	40.0	36.0	41.0	33.0	41.0
27	37.75360598163167	40.0	36.0	41.0	33.0	41.0
28	37.65816134826911	40.0	36.0	41.0	33.0	41.0
29	37.63505767340195	40.0	36.0	41.0	33.0	41.0
30	37.486491401747614	40.0	36.0	41.0	33.0	41.0
31	37.44564562812794	40.0	36.0	41.0	33.0	41.0
32	37.31392414184757	40.0	35.0	41.0	33.0	41.0
33	37.241658674343874	40.0	35.0	41.0	32.0	41.0
34	37.11123082914845	40.0	35.0	41.0	32.0	41.0
35	37.00122501291067	40.0	35.0	41.0	31.0	41.0
36	36.87562687547933	40.0	35.0	41.0	31.0	41.0
37	36.79622922496487	40.0	35.0	41.0	31.0	41.0
38	36.71878233030397	40.0	35.0	41.0	31.0	41.0
39	36.63914962829265	39.0	35.0	41.0	31.0	41.0
40	36.57959753007201	39.0	35.0	41.0	30.0	41.0
41	36.43836932595123	39.0	35.0	41.0	30.0	41.0
42	36.4550236681416	39.0	35.0	41.0	30.0	41.0
43	36.363164856884524	39.0	35.0	41.0	30.0	41.0
44	36.356281451005486	39.0	35.0	41.0	30.0	41.0
45	36.33079123137817	39.0	35.0	41.0	30.0	41.0
46	36.1905649986017	39.0	35.0	41.0	30.0	41.0
47	36.08294401141973	39.0	35.0	41.0	30.0	41.0
48	36.0241914314293	39.0	35.0	41.0	30.0	41.0
49	36.01724111308036	39.0	35.0	41.0	30.0	41.0
50	35.90190460340636	38.0	35.0	41.0	29.0	41.0
51	35.105038852125155	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	15.0
10	14.0
11	17.0
12	20.0
13	14.0
14	19.0
15	44.0
16	57.0
17	98.0
18	188.0
19	401.0
20	943.0
21	1604.0
22	2148.0
23	2928.0
24	3972.0
25	4758.0
26	5889.0
27	6356.0
28	6640.0
29	6980.0
30	8164.0
31	10393.0
32	13195.0
33	18629.0
34	34182.0
35	42639.0
36	40737.0
37	59903.0
38	105473.0
39	206231.0
40	194.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.139922553105336	18.387375161061748	21.58596279323532	13.886739492597593
2	32.121073825042764	27.344552896023167	23.281764979385812	17.252608299548257
3	31.531214667213405	26.735992560705906	25.31744819859621	16.415344573484475
4	28.5891248363647	23.948316121959127	28.68297386467554	18.77958517700064
5	28.541428255248768	28.344980106407984	24.107190345388442	19.006401292954802
6	25.858238211824293	36.401927765415174	23.579611255706862	14.160222767053673
7	77.2935106914117	5.9943278813967895	12.407630766696807	4.304530660494707
8	78.33923249681308	8.75197949390153	8.640115569845467	4.268672439439925
9	72.42811627671566	7.78655265239315	10.283760343552641	9.50157072733855
10	40.921607752238565	26.12966264105234	18.0311949366133	14.917534670095788
11	33.012896949649225	25.353821130958	23.984002772578243	17.649279146814536
12	27.769704435610475	22.70666087902397	27.75563565988563	21.767999025479927
13	26.990259946367086	23.0618116808584	30.132915616512623	19.815012756261893
14	21.368068339935935	28.851799173373642	28.75846485636981	21.021667630320614
15	20.74715493325052	25.268035913123594	32.66821194439059	21.316597209235294
16	24.170499836150235	25.013253816155416	29.17280745850998	21.64343888918437
17	24.52084666578594	26.0248331048587	28.225223942311157	21.229096287044204
18	24.750236338275133	24.626019342850917	28.146301541903508	22.477442776970445
19	24.860556128410177	26.609545149617997	26.50179891601799	22.028099805953836
20	27.78857718353404	25.975077678514747	27.83215607419392	18.40418906375729
21	25.487817641215337	27.610658641745488	28.01625115166655	18.885272565372624
22	24.186112745796095	24.052116235538755	29.84141744631132	21.920353572353825
23	24.763961973128637	26.41790097297594	28.351499782963398	20.466637270932022
24	23.051517454718272	26.211844879737704	28.46902553139653	22.26761213414749
25	23.499659432685196	28.50625631593666	26.735134708527564	21.258949542850576
26	21.947804842060837	26.649521061128826	28.811308550555804	22.591365546254533
27	24.529082046698043	25.953288233184814	27.52744698044612	21.99018273967103
28	21.02904515905437	28.52375650037488	28.85059818032396	21.596600160246787
29	23.097155190606173	25.70948664409943	29.225136441388965	21.968221723905422
30	23.162180385724653	27.09731989822442	28.340862415951936	21.399637300098995
31	23.323113454381993	26.797586347111015	26.752806463401452	23.12649373510554
32	25.261859377439517	26.994034495951798	27.300974005363294	20.443132121245394
33	25.007935132649685	25.88431691804595	27.105555279136524	22.002192670167847
34	22.18989072678952	26.783002860079165	28.166203712441085	22.86090270069023
35	23.65613167001515	26.214590006708406	27.474260145388783	22.65501817788766
36	23.776230974983314	29.016506791615697	25.228917853791106	21.978344379609883
37	23.28639738114887	27.801444966209203	27.05322629625753	21.858931356384392
38	22.39611839046343	27.500338851610444	26.997465904665173	23.106076853260955
39	23.344559758840596	26.536284573587416	27.53448136830854	22.584674299263447
40	23.510296799696665	25.048768896338856	28.639738114887	22.801196189077483
41	21.93888317940606	26.870503782270255	27.087197242519956	24.10341579580373
42	22.398177235691456	26.076647376430685	27.927720806861444	23.597454581016418
43	23.406153545245697	25.868189297093082	27.194257194377293	23.531399963283928
44	23.468262042957804	25.6799765291644	27.840906166413028	23.010855261464766
45	22.02895765813218	25.023548042295545	27.598305570377335	25.34918872919494
46	22.756416305367924	27.182418834316145	27.051510591900847	23.009654268415083
47	21.48988334926079	27.025603456114855	29.647028142698563	21.837485051925793
48	21.593683462840417	26.910822834652425	28.313582716680592	23.18191098582657
49	22.436609013281267	24.886291693760498	29.41111879365395	23.26598049930428
50	21.35622997987479	25.47769498551088	29.22307759616094	23.942997438453396
51	21.326719864939754	25.714633757169498	27.27369430609195	25.684952071798794
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	219.0
1	702.0
2	1185.0
3	2626.0
4	4067.0
5	2924.5
6	1782.0
7	1724.0
8	1666.0
9	1697.5
10	1729.0
11	1669.5
12	1610.0
13	1645.5
14	1681.0
15	1671.5
16	1662.0
17	1711.0
18	1760.0
19	1715.5
20	1671.0
21	1779.5
22	1888.0
23	2171.5
24	2455.0
25	2766.5
26	3858.0
27	4638.0
28	5504.0
29	6370.0
30	7767.0
31	9164.0
32	10498.5
33	11833.0
34	13303.0
35	14773.0
36	16067.5
37	17362.0
38	19044.5
39	20727.0
40	23412.5
41	26098.0
42	28373.0
43	30648.0
44	32779.5
45	34911.0
46	45131.5
47	55352.0
48	51208.5
49	47065.0
50	48043.0
51	49021.0
52	45626.5
53	42232.0
54	39692.5
55	37153.0
56	34485.0
57	31817.0
58	29680.0
59	27543.0
60	26450.0
61	25357.0
62	23107.5
63	20858.0
64	18055.5
65	15253.0
66	12666.5
67	10080.0
68	8521.0
69	6962.0
70	5840.0
71	4718.0
72	3743.5
73	2769.0
74	2260.5
75	1412.0
76	1072.0
77	800.5
78	529.0
79	378.0
80	227.0
81	156.5
82	86.0
83	53.5
84	21.0
85	11.5
86	2.0
87	1.0
88	0.0
89	2.0
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	582851.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.507238124212456
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.80796804542665	22.30963510298884
2	7.811656291500673	4.922474298416281
3	3.325422909128258	3.143246743848461
4	2.217540889106947	2.7947435537308154
5	1.7635724458919269	2.778264850100837
6	1.4627677012676468	2.765266217054797
7	1.315699294524841	2.901783568071675
8	1.1167011341993545	2.8147334839037756
9	1.0755482502329994	3.049879935075404
>10	9.016533688611904	46.99762963352923
>50	0.056447487729143896	1.2080016547592167
>100	0.02356545604226095	1.2425357215046413
>500	0.003836237030135504	0.768037644420879
>1k	0.0027401693072396454	2.303767592595181
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCG	3504	0.6011828065835008	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTC	3504	0.6011828065835008	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGC	3006	0.5157407296204347	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2104	0.3609841966471706	No Hit
GCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	1224	0.21000221325862012	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGATTACGT	780	0.13382493982166968	No Hit
GAACTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCT	749	0.12850625631593665	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCT	686	0.11769731886880182	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTT	665	0.11409433971975684	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.147113070064219E-4	0.0	0.0	0.061079075098095396	0.0
2	5.147113070064219E-4	0.0	0.0	0.39649927683061365	0.0
3	6.862817426752292E-4	0.0	0.0	0.51642701136311	0.0
4	6.862817426752292E-4	0.0	0.0	0.800376082394986	0.0
5	6.862817426752292E-4	0.0	0.0	1.832200682507193	0.0
6	6.862817426752292E-4	0.0	0.0	2.3678435826652096	0.0
7	6.862817426752292E-4	0.0	0.0	2.765543852545505	0.0
8	6.862817426752292E-4	0.0	0.0	3.2980984848614825	0.0
9	6.862817426752292E-4	0.0	0.0	3.494203492830929	0.0
10	6.862817426752292E-4	0.0	0.0	4.541812573024667	0.0
11	6.862817426752292E-4	0.0	0.0	5.130985449111351	0.0
12	6.862817426752292E-4	0.0	0.0	6.12506455337642	0.0
13	6.862817426752292E-4	0.0	0.0	6.370067135511477	0.0
14	6.862817426752292E-4	0.0	0.0	6.483646763924227	0.0
15	6.862817426752292E-4	0.0	0.0	6.7464926713688405	0.0
16	6.862817426752292E-4	0.0	0.0	7.088260979221104	0.0
17	6.862817426752292E-4	0.0	0.0	7.48458868561605	0.0
18	6.862817426752292E-4	0.0	0.0	7.910426506946029	0.0
19	6.862817426752292E-4	0.0	0.0	8.311215044668362	0.0
20	6.862817426752292E-4	0.0	0.0	8.60065436964164	0.0
21	6.862817426752292E-4	0.0	0.0	8.941907966186898	0.0
22	6.862817426752292E-4	0.0	0.0	9.31284324810286	0.0
23	6.862817426752292E-4	0.0	0.0	9.669881324729648	0.0
24	6.862817426752292E-4	0.0	0.0	9.965154044515666	0.0
25	6.862817426752292E-4	0.0	0.0	10.237264755486393	0.0
26	6.862817426752292E-4	0.0	0.0	10.506801909922089	0.0
27	6.862817426752292E-4	0.0	0.0	10.820604236760339	0.0
28	6.862817426752292E-4	0.0	0.0	11.09957776515782	0.0
29	6.862817426752292E-4	0.0	0.0	11.387472956210077	0.0
30	6.862817426752292E-4	0.0	0.0	11.734045236261068	0.0
31	6.862817426752292E-4	0.0	0.0	12.03875433000887	0.0
32	6.862817426752292E-4	0.0	0.0	12.3378015993796	0.0
33	6.862817426752292E-4	0.0	0.0	12.630672333066256	0.0
34	6.862817426752292E-4	0.0	0.0	12.929891172872654	0.0
35	6.862817426752292E-4	0.0	0.0	13.246781767552942	0.0
36	6.862817426752292E-4	0.0	0.0	13.538108367318577	0.0
37	6.862817426752292E-4	0.0	0.0	13.858087229840903	0.0
38	6.862817426752292E-4	0.0	0.0	14.195566276801447	0.0
39	6.862817426752292E-4	0.0	0.0	14.650056360888117	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	25	3.8884315E-5	45.0	1
GTTTACG	20	7.0304226E-4	45.0	1
ACGGGTC	55	1.8189894E-12	45.0	5
ACGGGTA	35	1.2105556E-7	45.0	5
CTCGGTC	20	7.0304226E-4	45.0	12
GCGATAC	20	7.0304226E-4	45.0	9
GTTATCG	20	7.0304226E-4	45.0	1
AAATGCG	20	7.0304226E-4	45.0	1
CCGATGA	975	0.0	42.0	18
CGATGAA	1015	0.0	41.674877	19
CGAATGC	125	0.0	39.6	45
ATTCCGG	40	3.4543154E-7	39.375	2
ATTTGCG	40	3.4543154E-7	39.375	1
GATGAAT	1100	0.0	39.272728	20
TCCGCTA	35	6.24299E-6	38.571426	2
ACGTCGA	70	0.0	38.571426	41
CCACCGA	1185	0.0	37.974686	15
CGTCGAA	125	0.0	37.8	42
TGATCGG	30	1.1392319E-4	37.499996	2
GACGTAA	30	1.1392319E-4	37.499996	18
>>END_MODULE