FastQCFastQC Report
Sat 18 Jun 2016
SRR3554334_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554334_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405619
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCG80501.9846210359968346No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGC76291.8808290538658199No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTC72791.7945411827355227No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25840.6370510257162509No Hit
GCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC24910.6141231056730577TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCTGTTTT19320.4763090486392403No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCT16840.4151679285240583No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTA11740.28943417344848243No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTC11380.28055884956079474No Hit
GAACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCT11090.27340928309571294No Hit
CCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC7150.17627379388046419TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGCT5580.13756752025915947TruSeq Adapter, Index 22 (95% over 23bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCGCCTGTTTT4750.11710496796254613No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTT4370.10773657052554245No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCTA650.045.00000430
CGCATGG406.7957444E-945.02
TCTCGAG253.8863276E-545.01
ATAAGCG207.0278824E-445.01
GATGCGA207.0278824E-445.09
GCGATCG406.7957444E-945.033
GCAATAT207.0278824E-445.09
GCTCGTC207.0278824E-445.042
TTCGCGC207.0278824E-445.021
CGATCGA406.7957444E-945.041
CCGTTCG253.8863276E-545.040
CGATCAC1200.044.99999634
TTCGAAT1450.043.44827343
CCGATGA17200.043.2994218
CGATGAA18450.043.29268319
TGAATGA20150.043.21339822
ATGAATG20350.043.1203921
GATGAAT20400.043.01470620
TCGAATG2350.042.1276644
ACCCGTC750.042.039