##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554332_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	603579
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76435893230215	33.0	31.0	34.0	30.0	34.0
2	31.85952791598117	33.0	31.0	34.0	30.0	34.0
3	31.871985274504247	33.0	31.0	34.0	30.0	34.0
4	35.56809961910537	37.0	35.0	37.0	33.0	37.0
5	35.35347485581838	37.0	35.0	37.0	33.0	37.0
6	35.505845962169	37.0	35.0	37.0	33.0	37.0
7	35.84436668605104	37.0	35.0	37.0	35.0	37.0
8	35.69389094053968	37.0	35.0	37.0	35.0	37.0
9	37.460642268866216	39.0	37.0	39.0	35.0	39.0
10	37.062467382065975	39.0	37.0	39.0	33.0	39.0
11	37.069142564602146	39.0	37.0	39.0	33.0	39.0
12	37.122275625891554	39.0	37.0	39.0	34.0	39.0
13	37.12512860785415	39.0	37.0	39.0	33.0	39.0
14	38.30154627646091	40.0	38.0	41.0	33.0	41.0
15	38.36287544795296	40.0	38.0	41.0	34.0	41.0
16	38.32148401452005	40.0	38.0	41.0	34.0	41.0
17	38.251960389609316	40.0	37.0	41.0	33.0	41.0
18	38.175269517329134	40.0	37.0	41.0	34.0	41.0
19	38.062248686584525	40.0	37.0	41.0	34.0	41.0
20	37.93194594245327	40.0	36.0	41.0	33.0	41.0
21	37.92608258405279	40.0	36.0	41.0	33.0	41.0
22	37.977537323200444	40.0	36.0	41.0	33.0	41.0
23	37.872363021244944	40.0	36.0	41.0	33.0	41.0
24	37.88377494909531	40.0	36.0	41.0	33.0	41.0
25	37.71404240372843	40.0	36.0	41.0	33.0	41.0
26	37.719759965141265	40.0	36.0	41.0	33.0	41.0
27	37.62535972921523	40.0	36.0	41.0	33.0	41.0
28	37.502401508336106	40.0	36.0	41.0	33.0	41.0
29	37.428214036604984	40.0	36.0	41.0	33.0	41.0
30	37.243101565826514	40.0	35.0	41.0	32.0	41.0
31	37.18300007124171	40.0	35.0	41.0	32.0	41.0
32	36.96856086775716	40.0	35.0	41.0	31.0	41.0
33	36.80412671746366	40.0	35.0	41.0	30.0	41.0
34	36.62355217792534	40.0	35.0	41.0	30.0	41.0
35	36.46197763673024	40.0	35.0	41.0	30.0	41.0
36	36.28579191787653	40.0	35.0	41.0	29.0	41.0
37	36.204922636473434	39.0	35.0	41.0	29.0	41.0
38	36.11837555647231	39.0	35.0	41.0	28.0	41.0
39	36.046774324487764	39.0	35.0	41.0	27.0	41.0
40	35.95616149667235	39.0	35.0	41.0	27.0	41.0
41	35.88907500095265	39.0	35.0	41.0	26.0	41.0
42	35.868598808109624	39.0	35.0	41.0	26.0	41.0
43	35.73739808707725	39.0	35.0	41.0	26.0	41.0
44	35.75681228140807	39.0	35.0	41.0	26.0	41.0
45	35.7524118632358	39.0	35.0	41.0	26.0	41.0
46	35.624834528702955	39.0	35.0	41.0	26.0	41.0
47	35.4789083119194	39.0	35.0	41.0	26.0	41.0
48	35.44416223891156	39.0	35.0	41.0	26.0	41.0
49	35.428452613493846	39.0	35.0	41.0	26.0	41.0
50	35.27816574135283	39.0	35.0	41.0	25.0	41.0
51	34.39223034598619	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	2.0
10	24.0
11	9.0
12	10.0
13	19.0
14	16.0
15	44.0
16	75.0
17	108.0
18	250.0
19	460.0
20	1025.0
21	1973.0
22	3010.0
23	3982.0
24	5311.0
25	6793.0
26	8372.0
27	8916.0
28	8843.0
29	8869.0
30	9756.0
31	11727.0
32	14584.0
33	18947.0
34	30258.0
35	39876.0
36	43465.0
37	62254.0
38	111817.0
39	202586.0
40	189.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.8507983213465	18.243676469857302	22.113426742812457	14.792098465983742
2	34.19187877643191	27.801994436519493	22.58312499275157	15.423001794297017
3	28.328851732747495	28.641983899373567	28.55434002839728	14.474824339481659
4	25.237789916481525	23.745524612353975	33.964236661646616	17.05244880951789
5	28.191338664864084	27.02661954773112	26.862432258246226	17.91960952915857
6	23.328346413642624	36.211995447157705	26.670576676789615	13.789081462410058
7	74.68169038352892	4.473482344481833	16.652832520680803	4.191994751308445
8	74.54318324527527	8.955414287110717	11.812206852789775	4.68919561482424
9	67.92350297144202	7.617395568765646	13.534599447628231	10.924502012164108
10	32.987065487699205	33.00827232226436	20.498725104750164	13.505937085286268
11	25.683299120744756	23.239542793901048	33.221831773471244	17.85532631188295
12	21.724579549652987	20.689255259046455	35.836236847206415	21.749928344094144
13	24.028503311082723	22.290868303900567	37.39609893651038	16.284529448506326
14	19.80502966471663	28.768562193184323	32.17341888965653	19.252989252442514
15	17.653861383514005	24.140170549339853	37.808803818555646	20.397164248590492
16	20.686273047935728	24.88274111591026	31.35563033173785	23.075355504416155
17	20.734651139287482	25.121980718348386	32.29121622853015	21.852151913833982
18	21.880979954570982	22.384310918703267	33.07487503707054	22.659834089655206
19	20.62762289609148	25.102927702918755	30.919399117596868	23.350050283392896
20	22.975285753811843	24.914054332572867	32.91251020993109	19.19814970368419
21	21.625835226209	27.89030102107595	31.761376721191425	18.722487031523627
22	20.078067659742967	22.739028362484447	33.21321649692915	23.969687480843437
23	19.82921871039251	26.433822250277096	31.926392402651516	21.810566636678878
24	19.913383335072957	25.956668472561173	31.24959615891209	22.880352033453782
25	18.93936005063132	29.561996027032087	29.790632212187635	21.708011710148963
26	19.698995491890873	24.208098691306358	32.829836690806005	23.263069125996765
27	22.505256147082655	24.36748130733508	31.011681983634286	22.115580561947983
28	18.79654527410662	26.524116975574035	32.75064241797676	21.928695332342578
29	21.955203875549017	22.954575954431814	32.59192251552821	22.498297654490962
30	20.905465564573984	26.201541140430663	32.8001802580938	20.09281303690155
31	21.22621893737191	25.950869728734766	28.92396852773208	23.898942806161248
32	24.008953260467976	25.958656613301656	28.15936273462132	21.87302739160905
33	21.97028060949768	24.099579342554993	30.282365688667102	23.647774359280227
34	22.53176469028909	23.905901298752937	29.160225919059478	24.40210809189849
35	23.434214908073344	23.20922364760868	30.020925181293585	23.33563626302439
36	24.067106377127104	26.93052607860777	28.74669264503901	20.25567489922612
37	21.032872250359937	24.96574599182543	30.88676047377394	23.114621284040698
38	20.834389533101717	26.753747231099823	28.631877517276116	23.779985718522347
39	23.065911835898863	24.109188689467327	27.881023030953695	24.943876443680114
40	23.416321641408995	22.554793987199687	30.799447959587727	23.22943641180359
41	22.296832726122016	23.976645973435126	28.061281124757485	25.665240175685373
42	23.786115819138836	24.247364470930897	29.810844976382544	22.15567473354772
43	24.013923612319182	23.7509919993903	28.805508475278295	23.429575913012215
44	21.59535040152159	23.65473285187192	30.34085016211631	24.409066584490184
45	21.204680746016678	22.743170322360452	29.343466223973998	26.708682707648872
46	24.120454820330064	24.659903674581123	28.60851686357544	22.61112464151337
47	20.233473994290723	24.493396887565673	32.88583598832962	22.387293129813994
48	21.731206685454595	24.30137562771402	29.349264967800405	24.61815271903098
49	22.337589611301915	21.83094507926883	32.66995703959217	23.16150826983709
50	22.236360111932324	21.801785681741745	31.748122449588205	24.213731756737726
51	21.698899398421748	21.49677175647264	29.902133772049723	26.90219507305589
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	214.0
1	563.5
2	913.0
3	4740.0
4	8567.0
5	5879.5
6	3192.0
7	3020.5
8	2849.0
9	2863.0
10	2877.0
11	2837.0
12	2797.0
13	2827.0
14	2857.0
15	2709.0
16	2561.0
17	2443.0
18	2325.0
19	2318.0
20	2311.0
21	2526.0
22	2741.0
23	2850.5
24	2960.0
25	3491.5
26	4631.0
27	5239.0
28	6421.0
29	7603.0
30	8924.0
31	10245.0
32	11241.0
33	12237.0
34	14230.0
35	16223.0
36	17896.5
37	19570.0
38	20716.0
39	21862.0
40	23866.0
41	25870.0
42	27772.0
43	29674.0
44	32681.0
45	35688.0
46	38696.0
47	41704.0
48	59095.0
49	76486.0
50	68158.5
51	59831.0
52	57179.5
53	54528.0
54	45798.0
55	37068.0
56	32731.0
57	28394.0
58	25124.0
59	21854.0
60	19258.5
61	16663.0
62	14774.0
63	12885.0
64	11405.5
65	9926.0
66	8291.5
67	6657.0
68	5368.5
69	4080.0
70	3443.5
71	2807.0
72	2441.0
73	2075.0
74	1779.0
75	1172.5
76	862.0
77	668.5
78	475.0
79	376.0
80	277.0
81	178.5
82	80.0
83	56.5
84	33.0
85	18.0
86	3.0
87	6.5
88	10.0
89	5.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	603579.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.74355954387127
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.52530523239938	28.12850295564247
2	8.47420825404683	6.39693567647564
3	3.429530650543426	3.8832808294895225
4	2.1819318917395596	3.2941550510617508
5	1.579526281591663	2.980847213018226
6	1.2776683007519827	2.8934249752049572
7	1.0376636687928127	2.741558432871541
8	0.9259067465063053	2.795761313506629
9	0.8330753249712537	2.829890531932418
>10	5.634449100745252	34.037244163572645
>50	0.06378388405322293	1.6770661220211998
>100	0.02947255331558003	1.8128784262675484
>500	0.0030792219881949283	0.9186423969720987
>1k	0.00263933313273851	1.5962096519952569
>5k	0.0017595554218256732	4.013602259968103
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTC	7245	1.2003399720666226	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCG	6198	1.0268746924594792	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGC	5556	0.9205091628436376	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5175	0.8573856943333019	No Hit
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC	2715	0.449816842534283	No Hit
GAACTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT	1586	0.262765934533839	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCT	1436	0.23791417527780126	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTACTCGGT	1419	0.23509764256211696	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTT	1279	0.21190266725648177	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTC	1179	0.19533482775245659	No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC	966	0.160045329608883	No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT	955	0.15822286726344023	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTACTCGGTCGTA	868	0.14380884689493836	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	811	0.13436517837764403	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	737	0.12210497714466541	No Hit
CGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG	635	0.10520578085055975	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6567839504025156E-4	0.0	0.0	0.16700382220057358	0.0
2	1.6567839504025156E-4	0.0	0.0	0.9553016258020905	0.0
3	1.6567839504025156E-4	0.0	0.0	1.3115101751386313	0.0
4	1.6567839504025156E-4	0.0	0.0	1.9377745083907822	0.0
5	1.6567839504025156E-4	0.0	0.0	4.062434246386968	0.0
6	1.6567839504025156E-4	0.0	0.0	5.04474145058062	0.0
7	1.6567839504025156E-4	0.0	0.0	5.952659055401199	0.0
8	1.6567839504025156E-4	0.0	0.0	7.234181441037545	0.0
9	1.6567839504025156E-4	0.0	0.0	7.795002808248796	0.0
10	1.6567839504025156E-4	0.0	0.0	9.820752544405952	0.0
11	1.6567839504025156E-4	0.0	0.0	10.976856384996827	0.0
12	1.6567839504025156E-4	0.0	0.0	12.91496225017769	0.0
13	1.6567839504025156E-4	0.0	0.0	13.444470400726335	0.0
14	1.6567839504025156E-4	0.0	0.0	13.686360857485102	0.0
15	3.3135679008050313E-4	0.0	0.0	14.138662875944988	0.0
16	3.3135679008050313E-4	0.0	0.0	14.754323791914564	0.0
17	4.970351851207547E-4	0.0	0.0	15.556869937489541	0.0
18	4.970351851207547E-4	0.0	0.0	16.46561593428532	0.0
19	4.970351851207547E-4	0.0	0.0	17.190293234191383	0.0
20	6.627135801610063E-4	0.0	0.0	17.714831032888817	0.0
21	6.627135801610063E-4	0.0	0.0	18.202256871097237	0.0
22	6.627135801610063E-4	0.0	0.0	18.767385876579535	0.0
23	6.627135801610063E-4	0.0	0.0	19.29722538391826	0.0
24	6.627135801610063E-4	0.0	0.0	19.716060366580017	0.0
25	6.627135801610063E-4	0.0	0.0	20.10623298689981	0.0
26	6.627135801610063E-4	0.0	0.0	20.525730683141727	0.0
27	6.627135801610063E-4	0.0	0.0	20.96742928431904	0.0
28	6.627135801610063E-4	0.0	0.0	21.35809893982395	0.0
29	6.627135801610063E-4	0.0	0.0	21.78455512865756	0.0
30	6.627135801610063E-4	0.0	0.0	22.288548806370002	0.0
31	6.627135801610063E-4	0.0	0.0	22.716827457549055	0.0
32	6.627135801610063E-4	0.0	0.0	23.118597565521664	0.0
33	6.627135801610063E-4	0.0	0.0	23.542071543244546	0.0
34	6.627135801610063E-4	0.0	0.0	23.976314616645045	0.0
35	6.627135801610063E-4	0.0	0.0	24.414202614736432	0.0
36	6.627135801610063E-4	0.0	0.0	24.8166354362892	0.0
37	8.283919752012578E-4	0.0	0.0	25.208299162164355	0.0
38	8.283919752012578E-4	0.0	0.0	25.610234948532007	0.0
39	8.283919752012578E-4	0.0	0.0	26.044312343537467	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCGGT	30	2.1637825E-6	45.000004	28
CTATGCG	25	3.8885966E-5	45.0	1
ACGTTGT	20	7.030621E-4	45.0	16
GTTTACG	35	1.2106466E-7	45.0	1
TTGTCCG	20	7.030621E-4	45.0	24
GCCGATT	20	7.030621E-4	45.0	9
AATTGCG	20	7.030621E-4	45.0	1
CGGTCTA	20	7.030621E-4	45.0	31
ATACGGT	20	7.030621E-4	45.0	28
TAGTGCG	35	1.2106466E-7	45.0	1
CGCTTTC	20	7.030621E-4	45.0	5
GTACGAG	20	7.030621E-4	45.0	1
ACGGTCT	20	7.030621E-4	45.0	30
GCATACG	20	7.030621E-4	45.0	1
CGTTTTT	3065	0.0	43.311584	1
CGATGAA	905	0.0	41.519333	19
CCGATGA	885	0.0	41.18644	18
CTAACGG	55	6.002665E-11	40.909092	2
CCGTACA	55	6.002665E-11	40.909092	41
TACGAAT	110	0.0	40.909092	12
>>END_MODULE