##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554331_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	904672
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.844069452796152	33.0	31.0	34.0	30.0	34.0
2	31.943477857168123	33.0	31.0	34.0	30.0	34.0
3	31.96309049025503	33.0	31.0	34.0	30.0	34.0
4	35.61690093205051	37.0	35.0	37.0	33.0	37.0
5	35.41610661101482	37.0	35.0	37.0	33.0	37.0
6	35.56312895723533	37.0	35.0	37.0	33.0	37.0
7	35.90264537865657	37.0	35.0	37.0	35.0	37.0
8	35.79755646245269	37.0	35.0	37.0	35.0	37.0
9	37.567039766898944	39.0	37.0	39.0	35.0	39.0
10	37.14658793463266	39.0	37.0	39.0	34.0	39.0
11	37.21762473028899	39.0	37.0	39.0	34.0	39.0
12	37.186844513812744	39.0	37.0	39.0	34.0	39.0
13	37.18740383254926	39.0	37.0	39.0	34.0	39.0
14	38.28396479608079	40.0	38.0	41.0	33.0	41.0
15	38.42441017296877	40.0	38.0	41.0	34.0	41.0
16	38.34529641682289	40.0	38.0	41.0	34.0	41.0
17	38.335769206961196	40.0	38.0	41.0	34.0	41.0
18	38.268856557956916	40.0	37.0	41.0	34.0	41.0
19	38.124528005730255	40.0	37.0	41.0	34.0	41.0
20	38.133353303738815	40.0	37.0	41.0	34.0	41.0
21	38.021413285699126	40.0	36.0	41.0	34.0	41.0
22	38.088985842382655	40.0	36.0	41.0	34.0	41.0
23	38.0840735647837	40.0	36.0	41.0	34.0	41.0
24	38.00700695942839	40.0	36.0	41.0	34.0	41.0
25	37.77130716989141	40.0	36.0	41.0	33.0	41.0
26	37.845733039156734	40.0	36.0	41.0	33.0	41.0
27	37.84358308867744	40.0	36.0	41.0	33.0	41.0
28	37.705855823989246	40.0	36.0	41.0	33.0	41.0
29	37.65220322945775	40.0	36.0	41.0	33.0	41.0
30	37.44153240069329	40.0	35.0	41.0	33.0	41.0
31	37.36501848183651	40.0	35.0	41.0	33.0	41.0
32	37.246546814757174	40.0	35.0	41.0	32.0	41.0
33	37.154367549786	40.0	35.0	41.0	32.0	41.0
34	36.98372780411022	40.0	35.0	41.0	31.0	41.0
35	36.852083407024864	40.0	35.0	41.0	31.0	41.0
36	36.68978370061193	40.0	35.0	41.0	31.0	41.0
37	36.68485373704503	40.0	35.0	41.0	31.0	41.0
38	36.53656684411588	39.0	35.0	41.0	30.0	41.0
39	36.48989357468784	39.0	35.0	41.0	30.0	41.0
40	36.39022872378055	39.0	35.0	41.0	30.0	41.0
41	36.25903974036999	39.0	35.0	41.0	30.0	41.0
42	36.30604683244314	39.0	35.0	41.0	30.0	41.0
43	36.21173088146864	39.0	35.0	41.0	30.0	41.0
44	36.14074161685119	39.0	35.0	41.0	30.0	41.0
45	36.08305330550741	39.0	35.0	41.0	30.0	41.0
46	35.933800316578825	39.0	35.0	41.0	29.0	41.0
47	35.90155990237346	39.0	35.0	41.0	29.0	41.0
48	35.80623474585264	38.0	35.0	41.0	29.0	41.0
49	35.800106557956916	38.0	35.0	41.0	29.0	41.0
50	35.70100102578614	38.0	35.0	41.0	29.0	41.0
51	34.89760598316296	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	10.0
10	18.0
11	12.0
12	30.0
13	28.0
14	47.0
15	73.0
16	101.0
17	166.0
18	256.0
19	542.0
20	897.0
21	1469.0
22	2164.0
23	3346.0
24	5023.0
25	7727.0
26	10531.0
27	12006.0
28	12326.0
29	12594.0
30	14120.0
31	17008.0
32	21582.0
33	29422.0
34	51075.0
35	64607.0
36	69065.0
37	98037.0
38	168314.0
39	301818.0
40	250.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.20099218280216	18.265183403487672	22.430671005624138	15.103153408086026
2	37.458880124509214	22.437524318205934	22.414532559867002	17.68906299741785
3	32.64674931909023	22.934389480386262	27.497037600367868	16.921823600155637
4	27.939076261893813	25.095283152346926	27.67643963779138	19.28920094796788
5	24.816397545187648	28.806241378090625	26.773349899189984	19.604011177531746
6	24.776493579993634	33.70414912808178	26.70603268366878	14.81332460825581
7	77.33366347140179	5.738764988857841	12.259581373138552	4.667990166601818
8	76.53127321283294	5.585228679565633	11.968536663011568	5.914961444589863
9	69.7394193696721	7.70234958084256	14.973272080930988	7.584958968554349
10	34.53030490608751	26.28599094478441	22.24176276042588	16.941941388702205
11	27.946040111775318	25.78978900640232	27.689040889957905	18.575129991864454
12	24.51949435817622	22.895480350889606	32.150215768809026	20.434809522125146
13	24.471631707403347	23.134240918255454	33.79467917654133	18.599448197799866
14	21.468996498178345	25.46779385235754	33.09840472569064	19.964804923773478
15	20.80079852145308	23.926793357150437	35.17186339358353	20.100544727812952
16	24.09370467970712	24.248235824696685	31.607919776449368	20.050139719146827
17	23.70361854904319	23.576169042481695	30.359511513565135	22.36070089490998
18	26.08260231332461	21.805472038484666	30.97144600473984	21.140479643450885
19	23.810618655158997	25.32298998974214	29.22915708676736	21.637234268331508
20	24.791858264652824	24.957664214212443	29.150786141275514	21.09969137985922
21	23.966918396943864	26.242218174100667	29.701261893813445	20.08960153514202
22	22.51556365179866	23.51283116974992	30.144516465636162	23.827088712815254
23	21.48612977963284	25.936250928513317	29.999491528421352	22.578127763432494
24	21.263397120724417	25.09495154044781	30.569974532206146	23.07167680662163
25	22.414974709065827	25.535442679777866	29.361359697216226	22.688222913940077
26	20.898071345194722	24.932903859078205	29.719832160164128	24.449192635562945
27	22.874588801245093	23.55781985073043	30.447057054932618	23.12053429309186
28	21.056360758374307	25.328959003926283	30.870525450107884	22.744154787591526
29	21.982663329914047	24.461241201230944	29.60874217395918	23.94735329489583
30	23.138330798344594	24.090830709914755	30.823436560432953	21.9474019313077
31	22.991205652435358	24.68596353153408	29.12005677195713	23.202774044073433
32	22.037268755969013	24.2243597679601	30.213270666053553	23.52510081001733
33	21.200501397191466	24.317542711612607	30.260691167627606	24.22126472356832
34	20.08960153514202	24.904385235753953	31.441229528492094	23.564783700611937
35	19.99376569629656	24.580400410314457	31.179587740087015	24.24624615330197
36	20.968373067808002	26.875596901418415	29.54883095751831	22.60719907325528
37	20.54192016554066	25.593806374022847	30.540129461285414	23.324143999151072
38	20.479466591206535	26.538789749212977	29.44205192600191	23.53969173357858
39	22.156206890453113	24.230881468642778	28.88582823387924	24.727083407024868
40	22.701597927204556	23.29617806232535	29.780296063103535	24.22192794736656
41	20.125084008347777	24.093925754306532	29.282657139825265	26.498333097520426
42	21.531560609812175	23.983277917300413	30.07532011601995	24.409841356867464
43	21.500168016695554	23.83637384599059	30.995764210675254	23.667693926638606
44	21.89434402744862	23.926793357150437	30.04646988079658	24.132392734604366
45	21.50834777687383	22.94334300166248	29.632065544197232	25.916243677266458
46	21.969509391248984	24.19970995012557	28.90428796293021	24.926492695695234
47	20.218488026599697	24.718793109546887	31.070708499876197	23.99201036397722
48	21.10433394644689	24.106748081072478	30.492266810512543	24.296651161968093
49	22.453883838562486	22.9212355417212	30.61728449648049	24.007596123235825
50	20.84921385872449	22.822304658483958	31.256632237982384	25.07184924480917
51	21.32772982915355	22.474554313607584	29.446362880690458	26.75135297654841
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	281.0
1	778.5
2	1276.0
3	7223.5
4	13171.0
5	8616.0
6	4061.0
7	3943.5
8	3826.0
9	3754.5
10	3683.0
11	3678.0
12	3673.0
13	3676.0
14	3679.0
15	3693.0
16	3707.0
17	3504.5
18	3302.0
19	3278.5
20	3255.0
21	3395.5
22	3536.0
23	3901.0
24	4266.0
25	4731.5
26	5801.5
27	6406.0
28	7535.5
29	8665.0
30	10032.0
31	11399.0
32	13703.0
33	16007.0
34	17540.5
35	19074.0
36	21247.0
37	23420.0
38	25553.5
39	27687.0
40	32038.0
41	36389.0
42	41886.0
43	47383.0
44	59747.5
45	72112.0
46	78579.5
47	85047.0
48	85782.5
49	86518.0
50	79201.0
51	71884.0
52	67849.5
53	63815.0
54	58006.0
55	52197.0
56	49575.5
57	46954.0
58	43458.0
59	39962.0
60	36721.0
61	33480.0
62	30600.0
63	27720.0
64	24982.0
65	22244.0
66	18789.5
67	15335.0
68	13368.5
69	11402.0
70	9663.0
71	7924.0
72	6834.0
73	5744.0
74	4973.5
75	3267.0
76	2331.0
77	1812.0
78	1293.0
79	968.5
80	644.0
81	469.5
82	295.0
83	230.5
84	166.0
85	102.0
86	38.0
87	24.5
88	11.0
89	9.0
90	7.0
91	4.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	904672.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.69773663460147
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.8058561224884	25.608961578293908
2	8.65211996110318	6.004179594826729
3	3.3668187175126914	3.5046296747010612
4	1.9761183499666668	2.7426733626378645
5	1.428510496321555	2.478304049056457
6	1.1236917674337539	2.339373660293174
7	0.9665976810143424	2.347712623721652
8	0.8329514698887817	2.31212245852841
9	0.7263270374766878	2.268171383126078
>10	7.01087607905215	44.013790543924166
>50	0.07299471982060408	1.713161420272095
>100	0.0320151706217386	2.0196435820254837
>500	0.002881365355939756	0.7453830061793139
>1k	0.0019209102372931709	0.9976579401634007
>5k	3.2015170621552846E-4	0.9042351222503152
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8140	0.8997736196102012	No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	2332	0.25777298291535494	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1522	0.1682377701531605	No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	1493	0.1650321884616745	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTC	1403	0.15508383148809735	No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT	1208	0.13352905804534684	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG	1023	0.11307965759966042	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCG	945	0.10445774822256022	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1053729970641293E-4	0.0	0.0	0.138392699232429	0.0
2	1.1053729970641293E-4	0.0	0.0	0.6949480032542181	0.0
3	1.1053729970641293E-4	0.0	0.0	1.0621529128789218	0.0
4	1.1053729970641293E-4	0.0	0.0	1.4992173959180786	0.0
5	1.1053729970641293E-4	0.0	0.0	2.6287980616179123	0.0
6	1.1053729970641293E-4	0.0	0.0	3.696256764882742	0.0
7	1.1053729970641293E-4	0.0	0.0	4.605426054967988	0.0
8	2.2107459941282586E-4	0.0	0.0	6.159248876941035	0.0
9	2.2107459941282586E-4	0.0	0.0	6.998226981712709	0.0
10	2.2107459941282586E-4	0.0	0.0	8.346450426231828	0.0
11	2.2107459941282586E-4	0.0	0.0	9.555728485019985	0.0
12	2.2107459941282586E-4	0.0	0.0	10.634572530154575	0.0
13	2.2107459941282586E-4	0.0	0.0	11.1089986204945	0.0
14	2.2107459941282586E-4	0.0	0.0	11.327862473913198	0.0
15	2.2107459941282586E-4	0.0	0.0	11.654610731845354	0.0
16	2.2107459941282586E-4	0.0	0.0	12.219787945244244	0.0
17	2.2107459941282586E-4	0.0	0.0	12.914625411198754	0.0
18	3.316118991192388E-4	0.0	0.0	13.701540447808709	0.0
19	3.316118991192388E-4	0.0	0.0	14.117934455802766	0.0
20	3.316118991192388E-4	0.0	0.0	14.541071239078915	0.0
21	3.316118991192388E-4	0.0	0.0	15.007096494641152	0.0
22	3.316118991192388E-4	0.0	0.0	15.491139330055534	0.0
23	3.316118991192388E-4	0.0	0.0	15.95473276502423	0.0
24	3.316118991192388E-4	0.0	0.0	16.33807612040607	0.0
25	3.316118991192388E-4	0.0	0.0	16.693121927063068	0.0
26	3.316118991192388E-4	0.0	0.0	17.04374624173181	0.0
27	3.316118991192388E-4	0.0	0.0	17.45914541402851	0.0
28	3.316118991192388E-4	0.0	0.0	17.819828622970537	0.0
29	3.316118991192388E-4	0.0	0.0	18.216989140815677	0.0
30	3.316118991192388E-4	0.0	0.0	18.722807824272223	0.0
31	3.316118991192388E-4	0.0	0.0	19.122842311909732	0.0
32	3.316118991192388E-4	0.0	0.0	19.5687497789254	0.0
33	4.421491988256517E-4	0.0	0.0	19.984812175020338	0.0
34	4.421491988256517E-4	0.0	0.0	20.404301227406176	0.0
35	4.421491988256517E-4	0.0	0.0	20.837828516854728	0.0
36	4.421491988256517E-4	0.0	0.0	21.21951381274097	0.0
37	5.526864985320646E-4	0.0	0.0	21.59644600473984	0.0
38	5.526864985320646E-4	0.0	0.0	22.037821442467546	0.0
39	5.526864985320646E-4	0.0	0.0	22.611620565243534	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGAA	45	3.8380676E-10	45.0	11
GTATACG	25	3.8901453E-5	45.0	1
TGTCGTG	20	7.03249E-4	45.0	40
GCGTAAG	65	0.0	41.53846	1
CGTTTTT	4310	0.0	41.397915	1
GCTACGA	50	1.0804797E-9	40.5	10
GCGTACG	40	3.457135E-7	39.375	1
GCCGATA	35	6.2468134E-6	38.57143	9
TGATTCG	70	0.0	38.57143	15
GCTTACG	35	6.2468134E-6	38.57143	1
ATAACGG	35	6.2468134E-6	38.57143	2
CTAACGG	65	9.094947E-12	38.07692	2
CGTCATA	30	1.13972994E-4	37.499996	38
TCTACGG	55	2.748493E-9	36.81818	2
TACGAGG	130	0.0	36.346153	2
TCGGCGT	280	0.0	36.160717	4
GTCGGCG	280	0.0	36.160717	3
TAGGGCG	375	0.0	36.0	5
CATACGA	50	4.8770744E-8	36.0	18
TTACGTA	25	0.0021068722	36.0	12
>>END_MODULE