FastQCFastQC Report
Sat 18 Jun 2016
SRR3554323_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554323_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences854781
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36110.4224473871085108No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA30000.35096708981598795No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG18880.2208752885241951No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG15500.1813329964049271No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG12870.15056488153105885No Hit
CTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGCT9860.11535118351952138Illumina Single End Adapter 1 (95% over 21bp)
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA9030.10564109403461237No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG406.8121153E-945.0000041
ATTACCG406.8121153E-945.0000041
GTTAGCG207.0322695E-445.0000041
CGTTCGG207.0322695E-445.0000042
GCGTTAG207.0322695E-445.0000041
CGAATTA207.0322695E-445.00000410
CGCACGA207.0322695E-445.00000440
TATACCG207.0322695E-445.0000041
ATTACGC253.889963E-545.025
TAGCACG551.8189894E-1245.01
TAGATCG351.211447E-745.01
CGACCCG302.1648466E-644.99999632
CGTAGCG302.1648466E-644.9999961
TATCGCG302.1648466E-644.9999961
TACGAAT1200.041.24999611
CGTATAG501.0804797E-940.51
CACGACG501.0804797E-940.51
CTCAACG451.9272193E-840.0000041
TACAGCG451.9272193E-840.0000041
TACCGCG403.4568257E-739.3750041