##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554323_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	854781
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40839115516138	34.0	31.0	34.0	31.0	34.0
2	32.58224621277263	34.0	31.0	34.0	31.0	34.0
3	32.66385074071604	34.0	31.0	34.0	31.0	34.0
4	36.15319362503378	37.0	35.0	37.0	35.0	37.0
5	36.00501882938437	37.0	35.0	37.0	35.0	37.0
6	35.9877921947259	37.0	35.0	37.0	35.0	37.0
7	36.01108939014789	37.0	35.0	37.0	35.0	37.0
8	36.123973275026	37.0	36.0	37.0	35.0	37.0
9	37.75764903524996	39.0	38.0	39.0	35.0	39.0
10	37.40316525519402	39.0	37.0	39.0	35.0	39.0
11	37.38423526025964	39.0	37.0	39.0	35.0	39.0
12	37.35729268666477	39.0	37.0	39.0	35.0	39.0
13	37.34234733809011	39.0	37.0	39.0	34.0	39.0
14	38.627034292994345	40.0	38.0	41.0	35.0	41.0
15	38.62867447919409	40.0	38.0	41.0	34.0	41.0
16	38.621587283760405	40.0	38.0	41.0	34.0	41.0
17	38.586470686643715	40.0	38.0	41.0	34.0	41.0
18	38.508837936266715	40.0	38.0	41.0	34.0	41.0
19	38.525105260879684	40.0	38.0	41.0	34.0	41.0
20	38.438463185307114	40.0	38.0	41.0	34.0	41.0
21	38.398807413828806	40.0	38.0	41.0	34.0	41.0
22	38.354999701677976	40.0	38.0	41.0	34.0	41.0
23	38.28896641361939	40.0	38.0	41.0	34.0	41.0
24	38.25009680842228	40.0	38.0	41.0	34.0	41.0
25	38.20100587167941	40.0	37.0	41.0	34.0	41.0
26	38.14633221842788	40.0	37.0	41.0	34.0	41.0
27	38.07410904079524	40.0	37.0	41.0	34.0	41.0
28	37.974829810208696	40.0	37.0	41.0	33.0	41.0
29	37.90628125800644	40.0	37.0	41.0	33.0	41.0
30	37.86274496040506	40.0	37.0	41.0	33.0	41.0
31	37.785249087193094	40.0	37.0	41.0	33.0	41.0
32	37.74139574932059	40.0	37.0	41.0	33.0	41.0
33	37.674109508751364	40.0	37.0	41.0	33.0	41.0
34	37.58600039074336	40.0	36.0	41.0	33.0	41.0
35	37.49632361973418	40.0	36.0	41.0	33.0	41.0
36	37.41594396693422	40.0	36.0	41.0	32.0	41.0
37	37.303172391524846	40.0	36.0	41.0	32.0	41.0
38	37.26719007558661	40.0	36.0	41.0	32.0	41.0
39	37.21481291699278	39.0	35.0	41.0	32.0	41.0
40	37.080617140530734	39.0	35.0	41.0	31.0	41.0
41	36.988815848737865	39.0	35.0	41.0	31.0	41.0
42	36.9200801140877	39.0	35.0	41.0	31.0	41.0
43	36.877409535307876	39.0	35.0	41.0	31.0	41.0
44	36.815556265289004	39.0	35.0	41.0	31.0	41.0
45	36.689885479438594	39.0	35.0	41.0	31.0	41.0
46	36.59035823210858	39.0	35.0	41.0	31.0	41.0
47	36.47579555465084	39.0	35.0	41.0	30.0	41.0
48	36.36022326186473	39.0	35.0	40.0	30.0	41.0
49	36.236569366890464	39.0	35.0	40.0	30.0	41.0
50	36.12585562851771	38.0	35.0	40.0	29.0	41.0
51	35.10714206328873	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	4.0
11	6.0
12	5.0
13	9.0
14	19.0
15	30.0
16	53.0
17	87.0
18	168.0
19	320.0
20	537.0
21	887.0
22	1246.0
23	1881.0
24	2554.0
25	3373.0
26	4233.0
27	5354.0
28	6846.0
29	9169.0
30	12093.0
31	15758.0
32	20566.0
33	28004.0
34	43539.0
35	57209.0
36	69293.0
37	102646.0
38	178171.0
39	290587.0
40	131.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.207749119365076	32.70124160457474	20.925944774158527	26.165064501901657
2	21.48632222756472	31.846636740872807	21.556047689408164	25.11099334215431
3	21.480589765097726	31.047952633481557	21.98656731958244	25.484890281838275
4	21.94456825783446	31.367449674244046	19.69849587204208	26.989486195879415
5	19.560331827684518	34.27497803530963	17.91663595704631	28.248054179959546
6	20.617210724150397	37.90772139296498	18.84903852565745	22.626029357227175
7	85.39731229402618	6.390876727489263	4.300165773455423	3.911645205029125
8	86.83265070234364	5.863139213435956	3.9283746363103536	3.3758354479100494
9	76.85430537178529	11.651639425771046	6.988924648535706	4.50513055390796
10	32.0526544225948	41.29034220461148	13.537970544502043	13.11903282829169
11	24.96534199988067	28.82855374651519	28.87570032558047	17.330403928023667
12	27.1794763804998	24.752889921512057	29.33850892801782	18.72912476997032
13	24.004394107964497	26.628809016578515	28.00846064664516	21.358336228811826
14	19.58969607419912	28.980405507375572	29.114006979565527	22.31589143885978
15	20.07695538389365	30.511908898302607	29.624313128157976	19.78682258964577
16	22.208495509376085	29.3454112807842	28.60487072127247	19.841222488567247
17	21.15313747029941	29.341082686676472	28.647103761080324	20.8586760819438
18	20.664474292245615	30.606553023522988	27.972545014453992	20.756427669777405
19	22.33145097984162	29.79418119962891	28.060169797878054	19.814198022651418
20	23.663722052783108	28.378964904460908	28.85967282847887	19.097640214277106
21	23.48016626480935	29.25895638765953	27.697152837978383	19.56372450955274
22	22.357305555458066	29.12757770703841	27.43907503793369	21.07604169956983
23	22.134090486335094	28.416869350161033	28.569072078111233	20.879968085392633
24	20.803340270782808	28.854057355041817	29.462751277812682	20.879851096362696
25	20.43225106781737	31.371895257381716	27.365605927132215	20.8302477476687
26	21.76124644792058	31.22542499189851	26.5304212424001	20.482907317780814
27	21.55897241515663	29.744577850934917	27.54518408808806	21.15126564582039
28	21.649638913359095	30.115082108750663	28.392418642903856	19.84286033498639
29	21.84220285663813	29.707726306504238	27.108931995446788	21.341138841410842
30	21.25363104701672	29.83290456853861	28.06765709579413	20.84580728865054
31	22.02973627162981	29.943693179890523	27.438958048903757	20.587612499575915
32	23.011625199905005	29.086865524619753	28.187804829541136	19.713704445934106
33	21.169632923520762	30.980917919326707	27.640881114577887	20.208568042574647
34	20.823930340052012	29.531189860326794	29.534699531224955	20.11018026839623
35	23.289123178919514	27.750733813690292	28.776376639162542	20.18376636822765
36	22.986004602348437	30.680022134324464	27.463525745190875	18.870447518136224
37	21.135472126778673	31.949938054308646	27.808643383509928	19.10594643540275
38	21.42256320624815	29.246087594366276	29.260360256018792	20.07098894336678
39	22.6355054686522	28.7541487234742	28.135393744128613	20.474952063744983
40	22.782326701225227	29.029306921889937	29.16290839407989	19.02545798280495
41	19.691125563155943	29.19940897142075	30.857026536621664	20.25243892880165
42	20.337372964537114	29.764582975054427	28.540994710925954	21.357049349482498
43	21.527970322222885	29.212862709863696	29.674852389091477	19.584314578821942
44	21.667772212999587	29.259541332809224	28.084620505135234	20.988065949055958
45	20.331991469159938	29.575762680733426	28.17517001430776	21.917075835798876
46	19.581623831133356	30.2328900618989	28.751808942875428	21.433677164092323
47	21.102013264216215	28.955954800118395	28.96168726258539	20.980344673080005
48	20.893889779955334	28.113165828440266	29.798509793736645	21.194434597867758
49	19.899015069356945	30.169715985732015	28.711564716576525	21.21970422833451
50	19.913404720039402	29.481001566483112	29.623962161068157	20.98163155240933
51	21.115233024599284	27.851344379437542	29.210288951205044	21.82313364475813
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4565.0
1	3618.5
2	2672.0
3	1942.5
4	1213.0
5	1186.0
6	1159.0
7	1278.0
8	1397.0
9	1596.5
10	1796.0
11	1936.0
12	2076.0
13	2171.0
14	2266.0
15	2491.5
16	2717.0
17	2942.5
18	3168.0
19	3102.0
20	3036.0
21	3772.5
22	4509.0
23	6884.0
24	9259.0
25	9213.0
26	10910.0
27	12653.0
28	16033.5
29	19414.0
30	22795.0
31	26176.0
32	30667.5
33	35159.0
34	38821.5
35	42484.0
36	43491.0
37	44498.0
38	45699.0
39	46900.0
40	47522.0
41	48144.0
42	49791.5
43	51439.0
44	53406.0
45	55373.0
46	58553.0
47	61733.0
48	61267.5
49	60802.0
50	57820.5
51	54839.0
52	49171.0
53	43503.0
54	39931.0
55	36359.0
56	34046.5
57	31734.0
58	30740.0
59	29746.0
60	29024.5
61	28303.0
62	25788.5
63	23274.0
64	20129.0
65	16984.0
66	14279.5
67	11575.0
68	10123.5
69	8672.0
70	7281.5
71	5891.0
72	4965.0
73	4039.0
74	3395.0
75	2163.5
76	1576.0
77	1193.0
78	810.0
79	617.5
80	425.0
81	357.5
82	290.0
83	198.5
84	107.0
85	87.0
86	67.0
87	47.0
88	27.0
89	23.0
90	19.0
91	13.5
92	8.0
93	6.5
94	5.0
95	2.5
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	854781.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.595595967325455
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.41273602970921	17.810343982753185
2	8.431355617828803	4.147484324659138
3	3.3975147043431084	2.5069169688321087
4	1.9872661295710605	1.9551197920992178
5	1.3781192341563995	1.6947831939055389
6	1.119350704128408	1.6518658598690148
7	0.9377012107838556	1.6144324082958115
8	0.7692207361631218	1.5135553949085438
9	0.6853948428613791	1.5171925169796299
>10	7.740374105866141	40.650261484895715
>50	0.9261403213873842	15.502030570203809
>100	0.2071880925095148	7.1833609255746795
>500	0.005251311100471573	0.9216090736022043
>1k	0.0023869595911234424	1.3310435034214025
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3611	0.4224473871085108	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	3000	0.35096708981598795	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1888	0.2208752885241951	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1550	0.1813329964049271	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1287	0.15056488153105885	No Hit
CTGTCTCTTATACACATCTGACGCTGTAGGGTTCGTATGCCGTCTTCTGCT	986	0.11535118351952138	Illumina Single End Adapter 1 (95% over 21bp)
ATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	903	0.10564109403461237	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.339780598773253E-4	0.0	0.0	0.1393339346569472	0.0
2	2.339780598773253E-4	0.0	0.0	0.2214602336738884	0.0
3	2.339780598773253E-4	0.0	0.0	0.3172742491936531	0.0
4	2.339780598773253E-4	0.0	0.0	0.6917561340273123	0.0
5	2.339780598773253E-4	0.0	0.0	0.7791469393914933	0.0
6	2.339780598773253E-4	0.0	0.0	1.2660552819962072	0.0
7	2.339780598773253E-4	0.0	0.0	1.6252116039079016	0.0
8	2.339780598773253E-4	0.0	0.0	1.9394441383231495	0.0
9	2.339780598773253E-4	0.0	0.0	2.4089211154670025	0.0
10	2.339780598773253E-4	0.0	0.0	3.0494360543811805	0.0
11	2.339780598773253E-4	0.0	0.0	3.576705612314733	0.0
12	2.339780598773253E-4	0.0	0.0	3.8310397634013857	0.0
13	2.339780598773253E-4	0.0	0.0	3.9577388828249576	0.0
14	2.339780598773253E-4	0.0	0.0	4.112047413314054	0.0
15	2.339780598773253E-4	0.0	0.0	4.2181564634684205	0.0
16	2.339780598773253E-4	0.0	0.0	4.428034783178381	0.0
17	2.339780598773253E-4	0.0	0.0	4.672424866720248	0.0
18	2.339780598773253E-4	0.0	0.0	4.962089704848377	0.0
19	2.339780598773253E-4	0.0	0.0	5.124353489373301	0.0
20	2.339780598773253E-4	0.0	0.0	5.297731231742399	0.0
21	2.339780598773253E-4	0.0	0.0	5.495325703308801	0.0
22	2.339780598773253E-4	0.0	0.0	5.730473653485513	0.0
23	2.339780598773253E-4	0.0	0.0	5.9488921723809955	0.0
24	2.339780598773253E-4	0.0	0.0	6.130810113935616	0.0
25	2.339780598773253E-4	0.0	0.0	6.29061712883183	0.0
26	2.339780598773253E-4	0.0	0.0	6.443404801931723	0.0
27	2.339780598773253E-4	0.0	0.0	6.61163502698352	0.0
28	2.339780598773253E-4	0.0	0.0	6.772377954119242	0.0
29	2.339780598773253E-4	0.0	0.0	6.944936773278769	0.0
30	2.339780598773253E-4	0.0	0.0	7.175054195168119	0.0
31	2.339780598773253E-4	0.0	0.0	7.353930421944335	0.0
32	2.339780598773253E-4	0.0	0.0	7.5393580343971145	0.0
33	2.339780598773253E-4	0.0	0.0	7.724902635879833	0.0
34	2.339780598773253E-4	0.0	0.0	7.90623563228476	0.0
35	2.339780598773253E-4	0.0	0.0	8.10043742198294	0.0
36	2.339780598773253E-4	0.0	0.0	8.298967805788852	0.0
37	2.339780598773253E-4	0.0	0.0	8.492818628397215	0.0
38	2.339780598773253E-4	0.0	0.0	8.694975672131225	0.0
39	2.339780598773253E-4	0.0	0.0	8.924274170811003	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	40	6.8121153E-9	45.000004	1
ATTACCG	40	6.8121153E-9	45.000004	1
GTTAGCG	20	7.0322695E-4	45.000004	1
CGTTCGG	20	7.0322695E-4	45.000004	2
GCGTTAG	20	7.0322695E-4	45.000004	1
CGAATTA	20	7.0322695E-4	45.000004	10
CGCACGA	20	7.0322695E-4	45.000004	40
TATACCG	20	7.0322695E-4	45.000004	1
ATTACGC	25	3.889963E-5	45.0	25
TAGCACG	55	1.8189894E-12	45.0	1
TAGATCG	35	1.211447E-7	45.0	1
CGACCCG	30	2.1648466E-6	44.999996	32
CGTAGCG	30	2.1648466E-6	44.999996	1
TATCGCG	30	2.1648466E-6	44.999996	1
TACGAAT	120	0.0	41.249996	11
CGTATAG	50	1.0804797E-9	40.5	1
CACGACG	50	1.0804797E-9	40.5	1
CTCAACG	45	1.9272193E-8	40.000004	1
TACAGCG	45	1.9272193E-8	40.000004	1
TACCGCG	40	3.4568257E-7	39.375004	1
>>END_MODULE