FastQCFastQC Report
Sat 18 Jun 2016
SRR3554321_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554321_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486871
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58191.1951831183208694No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA22770.4676803506472967No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12270.25201747485473563No Hit
CTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGCT12000.24647185804864122No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10260.21073343863158822No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG9430.1936858017832239No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA8460.17376265992429207No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6850.14069435230276603No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6520.1339163762064284No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA6060.12446828831456382No Hit
CTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACTC5600.11502020042269924No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5480.11255548184221283No Hit
TGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA5440.11173390898205068No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT5380.11050154969180748No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG302.162913E-645.0000041
GTATTCG302.162913E-645.00000410
CAACCCG351.2099918E-745.0000041
ATATGGG1400.045.0000043
CGTTATG302.162913E-645.0000041
CCCAATG302.162913E-645.00000417
AAGCACG351.2099918E-745.0000041
TACAATC351.2099918E-745.00000436
CTAGCAG453.8380676E-1045.0000041
TCACGAC253.8874816E-545.025
AAGTACG207.0292765E-445.01
CGTATAG253.8874816E-545.01
GTTTCGT253.8874816E-545.042
CGTGCGT253.8874816E-545.012
CGTTGTA207.0292765E-445.032
TAGGTAT253.8874816E-545.014
CGTTGAG406.8012014E-945.01
CTCCCGA207.0292765E-445.020
CAACCGA207.0292765E-445.042
CCAGCGA207.0292765E-445.041