##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554321_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	486871
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24614322890458	34.0	31.0	34.0	30.0	34.0
2	32.42139909750221	34.0	31.0	34.0	30.0	34.0
3	32.513337208418655	34.0	31.0	34.0	30.0	34.0
4	36.048881531247496	37.0	35.0	37.0	35.0	37.0
5	35.875178435355565	37.0	35.0	37.0	35.0	37.0
6	35.84378819030092	37.0	35.0	37.0	35.0	37.0
7	35.76449819356667	37.0	35.0	37.0	35.0	37.0
8	35.921118735763685	37.0	35.0	37.0	35.0	37.0
9	37.56385777752218	39.0	37.0	39.0	35.0	39.0
10	37.24689291413947	39.0	37.0	39.0	34.0	39.0
11	37.24228799825826	39.0	37.0	39.0	34.0	39.0
12	37.1618929860271	39.0	37.0	39.0	34.0	39.0
13	37.108468156862905	39.0	37.0	39.0	33.0	39.0
14	38.344304754236745	40.0	38.0	41.0	34.0	41.0
15	38.35836186587412	40.0	38.0	41.0	34.0	41.0
16	38.35505503511197	40.0	38.0	41.0	34.0	41.0
17	38.29972621084435	40.0	38.0	41.0	34.0	41.0
18	38.20098547664576	40.0	37.0	41.0	34.0	41.0
19	38.15229496108825	40.0	37.0	41.0	34.0	41.0
20	38.063115281049804	40.0	37.0	41.0	33.0	41.0
21	38.00540800335202	40.0	37.0	41.0	33.0	41.0
22	37.95255005946134	40.0	37.0	41.0	33.0	41.0
23	37.8692795422196	40.0	37.0	41.0	33.0	41.0
24	37.809614867182475	40.0	36.0	41.0	33.0	41.0
25	37.76045810902683	40.0	36.0	41.0	33.0	41.0
26	37.68115578869968	40.0	36.0	41.0	33.0	41.0
27	37.61217653136046	40.0	36.0	41.0	33.0	41.0
28	37.47844500904757	40.0	36.0	41.0	33.0	41.0
29	37.40257070147945	39.0	36.0	41.0	32.0	41.0
30	37.323607279957116	39.0	36.0	41.0	32.0	41.0
31	37.23820478114326	39.0	35.0	41.0	32.0	41.0
32	37.16158900406884	39.0	35.0	41.0	32.0	41.0
33	37.08906876770233	39.0	35.0	41.0	31.0	41.0
34	36.98363221469342	39.0	35.0	41.0	31.0	41.0
35	36.90196581846115	39.0	35.0	41.0	31.0	41.0
36	36.81521799408878	39.0	35.0	41.0	31.0	41.0
37	36.7134497639005	39.0	35.0	41.0	31.0	41.0
38	36.69586399682873	39.0	35.0	41.0	31.0	41.0
39	36.64150257460395	39.0	35.0	41.0	31.0	41.0
40	36.53847323007532	39.0	35.0	41.0	30.0	41.0
41	36.4423594750971	39.0	35.0	41.0	30.0	41.0
42	36.31939671904878	39.0	35.0	40.0	30.0	41.0
43	36.22148988130326	39.0	35.0	40.0	30.0	41.0
44	36.14892651236159	38.0	35.0	40.0	30.0	41.0
45	36.00364572956697	38.0	35.0	40.0	29.0	41.0
46	35.8591372252609	38.0	35.0	40.0	29.0	41.0
47	35.73431566061647	38.0	35.0	40.0	28.0	41.0
48	35.625120411772315	38.0	34.0	40.0	28.0	41.0
49	35.54637265312578	38.0	34.0	40.0	28.0	41.0
50	35.42550696180302	38.0	34.0	40.0	27.0	41.0
51	34.336705205280246	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	0.0
12	3.0
13	5.0
14	6.0
15	18.0
16	30.0
17	74.0
18	158.0
19	291.0
20	495.0
21	818.0
22	1120.0
23	1672.0
24	2159.0
25	2653.0
26	3399.0
27	4169.0
28	5130.0
29	6310.0
30	8093.0
31	10711.0
32	14039.0
33	18686.0
34	29282.0
35	39145.0
36	41826.0
37	59867.0
38	96527.0
39	140131.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.704603478128703	34.4347886811907	19.622446192112488	26.238161648568102
2	22.34370089818453	33.75740185798703	19.54809384826782	24.35080339556063
3	21.1020989132645	32.39030461867723	20.302092340681618	26.205504127376656
4	22.315767420939018	32.925764730287895	18.73083424562153	26.02763360315155
5	18.80744591483165	35.282241086447954	17.64779582271279	28.262517176007606
6	20.05664744870818	39.61706488987843	19.120465174553424	21.20582248685997
7	79.21995764791906	9.592273928823035	6.2425159847269605	4.945252438530946
8	81.28313249300123	8.337937564570492	5.597170503069602	4.78175943935868
9	70.84833559608192	13.88417055031004	9.265082537263464	6.002411316344576
10	33.251312976127146	36.28640851478112	15.68238814798992	14.77989036110181
11	26.903841058514473	29.731900236407586	24.813759702261994	18.55049900281594
12	27.031801031484726	25.804987358047615	29.0037812890889	18.159430321378764
13	24.079478958492086	28.658104508175676	26.503735075615513	20.758681457716726
14	18.875636462225106	30.53930096473193	28.310373795112053	22.27468877793091
15	18.83496860564708	31.709220717602815	28.29373694469377	21.162073732056335
16	21.158376654185606	29.09497587656689	28.68932427686184	21.057323192385663
17	20.814753805422793	30.118039480683795	27.29203423494108	21.77517247895233
18	20.875139410644707	30.458170644790922	27.148875164057827	21.51781478050654
19	21.41860985764196	31.788913285038547	26.772389400888528	20.020087456430964
20	22.265856869684168	30.17719272661547	27.216860318236247	20.340090085464116
21	21.353910994904194	32.28062464184558	25.98840349907881	20.377060864171412
22	21.20561709364493	30.757633952320017	26.616495950672764	21.420253003362287
23	21.763670458910063	29.811592803843318	26.26876523760914	22.15597149963748
24	19.501469588453617	29.430999176373206	28.987966011530773	22.0795652236424
25	19.592869569146654	32.46588932181214	26.61074494065163	21.330496168389573
26	21.290033705026588	31.994511893294113	25.812586907004114	20.90286749467518
27	19.769096947651434	30.601329715674176	26.219060079569335	23.410513257105066
28	20.415264002168954	30.977815478843475	27.684335275668502	20.922585243319073
29	20.915191087577615	30.892577294601647	26.36221915045258	21.830012467368153
30	20.83036368976587	31.326778551197336	26.771362434813327	21.07149532422346
31	22.453380875016173	30.028898825356205	25.173197828582932	22.34452247104469
32	21.83966594847506	29.80912808526283	27.804490306467216	20.546715659794895
33	20.410745351438063	30.777351700963912	25.388860704375492	23.423042243222536
34	18.755481431426393	30.668082510562343	28.34426367559374	22.23217238241752
35	22.43961952960846	28.899236142633267	28.20582864865642	20.455315679101858
36	22.145496445670414	31.252836993782747	26.783480634500723	19.81818592604612
37	18.628959210961423	32.34676125708864	27.882334334967577	21.141945196982363
38	19.826607047862783	28.988992977605974	29.62386340529627	21.560536569234973
39	20.146404283680894	28.964961971446236	28.13620034875768	22.752433396115194
40	21.769421468931196	28.667757989282585	28.003927118271577	21.558893423514647
41	18.966420263273022	28.135789562327595	30.09955409133015	22.79823608306923
42	20.047815540461436	29.14036777709085	28.046032727354884	22.76578395509283
43	20.007969256743575	28.16043674813246	30.0810687019765	21.750525293147465
44	21.607571615479255	27.275191991307757	27.498454416056823	23.618781977156168
45	20.535829819397748	29.48604455800407	27.26615468984598	22.711970932752205
46	18.685031558667493	29.92332671282537	29.334053578874077	22.057588149633066
47	19.973257803401722	29.014872522701086	29.053281053913665	21.958588619983527
48	20.45593185874698	27.83057524477736	29.956805806876975	21.756687089598685
49	19.216794592407435	29.19274304692619	28.95530849033933	22.63515387032705
50	19.262802672576516	27.653320900197382	28.993511628336872	24.090364798889233
51	20.14229641938008	26.742607384707657	29.383142557268766	23.731953638643503
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7154.0
1	5501.0
2	3848.0
3	2494.0
4	1140.0
5	1019.5
6	899.0
7	997.5
8	1096.0
9	1062.0
10	1028.0
11	1246.5
12	1465.0
13	1496.0
14	1527.0
15	1701.5
16	1876.0
17	2091.0
18	2306.0
19	2371.0
20	2436.0
21	2713.0
22	2990.0
23	3997.0
24	5004.0
25	4901.0
26	5186.5
27	5575.0
28	7268.0
29	8961.0
30	11258.5
31	13556.0
32	15607.5
33	17659.0
34	18210.5
35	18762.0
36	18792.0
37	18822.0
38	20377.5
39	21933.0
40	22906.5
41	23880.0
42	25676.5
43	27473.0
44	31113.5
45	34754.0
46	36935.5
47	39117.0
48	38252.0
49	37387.0
50	35920.5
51	34454.0
52	30913.5
53	27373.0
54	24303.5
55	21234.0
56	20143.0
57	19052.0
58	17775.0
59	16498.0
60	16082.0
61	15666.0
62	14421.0
63	13176.0
64	11553.5
65	9931.0
66	8472.0
67	7013.0
68	6245.0
69	5477.0
70	4843.5
71	4210.0
72	3686.0
73	3162.0
74	2434.5
75	1423.0
76	1139.0
77	914.0
78	689.0
79	521.0
80	353.0
81	260.5
82	168.0
83	108.5
84	49.0
85	44.0
86	39.0
87	21.0
88	3.0
89	4.5
90	6.0
91	8.0
92	10.0
93	9.5
94	9.0
95	7.5
96	6.0
97	3.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	486871.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.684144131683414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.79068880811826	20.224096639953924
2	9.164043430922277	4.890693114794733
3	3.378348244023674	2.7044499441164183
4	1.7468252856490531	1.864501507805135
5	1.0929712397955254	1.4582501047244256
6	0.7288565353189441	1.1669347703862063
7	0.6013374834296832	1.1232323255735015
8	0.4956560308687992	1.0580925573952928
9	0.409306050639242	0.9829783484304879
>10	5.453480961604351	31.908995067304552
>50	0.7623307951281388	14.441894397527143
>100	0.3653637986761758	14.582376550281737
>500	0.00693728731663625	1.2180604811313378
>1k	0.003083238807393888	1.1785691585414666
>5k	7.70809701848472E-4	1.1968750320336463
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5819	1.1951831183208694	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2277	0.4676803506472967	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1227	0.25201747485473563	No Hit
CTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGCT	1200	0.24647185804864122	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1026	0.21073343863158822	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	943	0.1936858017832239	No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA	846	0.17376265992429207	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	685	0.14069435230276603	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	652	0.1339163762064284	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	606	0.12446828831456382	No Hit
CTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACTC	560	0.11502020042269924	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	548	0.11255548184221283	No Hit
TGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	544	0.11173390898205068	No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT	538	0.11050154969180748	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0539321504053435E-4	0.0	0.0	0.2912475789274777	0.0
2	2.0539321504053435E-4	0.0	0.0	0.4498111409387702	0.0
3	2.0539321504053435E-4	0.0	0.0	0.6348704276902917	0.0
4	2.0539321504053435E-4	0.0	0.0	1.4722585654105502	0.0
5	2.0539321504053435E-4	0.0	0.0	1.592413596209263	0.0
6	2.0539321504053435E-4	0.0	0.0	2.472728915872993	0.0
7	2.0539321504053435E-4	0.0	0.0	3.047008345126327	0.0
8	2.0539321504053435E-4	0.0	0.0	3.5105808314728133	0.0
9	2.0539321504053435E-4	0.0	0.0	4.217749670857373	0.0
10	2.0539321504053435E-4	0.0	0.0	5.395679759114837	0.0
11	2.0539321504053435E-4	0.0	0.0	6.526985587558101	0.0
12	2.0539321504053435E-4	0.0	0.0	7.078671763156976	0.0
13	2.0539321504053435E-4	0.0	0.0	7.291048347508888	0.0
14	2.0539321504053435E-4	0.0	0.0	7.580241994285961	0.0
15	2.0539321504053435E-4	0.0	0.0	7.805352957970387	0.0
16	2.0539321504053435E-4	0.0	0.0	8.235240957050225	0.0
17	2.0539321504053435E-4	0.0	0.0	8.711342429514183	0.0
18	2.0539321504053435E-4	0.0	0.0	9.31334994279799	0.0
19	2.0539321504053435E-4	0.0	0.0	9.609321565671399	0.0
20	2.0539321504053435E-4	0.0	0.0	9.934459025080566	0.0
21	2.0539321504053435E-4	0.0	0.0	10.287324568520203	0.0
22	2.0539321504053435E-4	0.0	0.0	10.665864263839909	0.0
23	2.0539321504053435E-4	0.0	0.0	11.019756773354748	0.0
24	2.0539321504053435E-4	0.0	0.0	11.314290643722876	0.0
25	2.0539321504053435E-4	0.0	0.0	11.591982270457677	0.0
26	2.0539321504053435E-4	0.0	0.0	11.829622220259576	0.0
27	2.0539321504053435E-4	0.0	0.0	12.107313846994378	0.0
28	2.0539321504053435E-4	0.0	0.0	12.374530419762113	0.0
29	2.0539321504053435E-4	0.0	0.0	12.655713731152606	0.0
30	2.0539321504053435E-4	0.0	0.0	12.997282647765013	0.0
31	2.0539321504053435E-4	0.0	0.0	13.270866410199005	0.0
32	2.0539321504053435E-4	0.0	0.0	13.563551741631766	0.0
33	2.0539321504053435E-4	0.0	0.0	13.859112578075095	0.0
34	2.0539321504053435E-4	0.0	0.0	14.153030268798101	0.0
35	2.0539321504053435E-4	0.0	0.0	14.43339200732843	0.0
36	2.0539321504053435E-4	0.0	0.0	14.71026206120307	0.0
37	2.0539321504053435E-4	0.0	0.0	14.991034586163481	0.0
38	2.0539321504053435E-4	0.0	0.0	15.280639019370634	0.0
39	2.0539321504053435E-4	0.0	0.0	15.584620977630625	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	30	2.162913E-6	45.000004	1
GTATTCG	30	2.162913E-6	45.000004	10
CAACCCG	35	1.2099918E-7	45.000004	1
ATATGGG	140	0.0	45.000004	3
CGTTATG	30	2.162913E-6	45.000004	1
CCCAATG	30	2.162913E-6	45.000004	17
AAGCACG	35	1.2099918E-7	45.000004	1
TACAATC	35	1.2099918E-7	45.000004	36
CTAGCAG	45	3.8380676E-10	45.000004	1
TCACGAC	25	3.8874816E-5	45.0	25
AAGTACG	20	7.0292765E-4	45.0	1
CGTATAG	25	3.8874816E-5	45.0	1
GTTTCGT	25	3.8874816E-5	45.0	42
CGTGCGT	25	3.8874816E-5	45.0	12
CGTTGTA	20	7.0292765E-4	45.0	32
TAGGTAT	25	3.8874816E-5	45.0	14
CGTTGAG	40	6.8012014E-9	45.0	1
CTCCCGA	20	7.0292765E-4	45.0	20
CAACCGA	20	7.0292765E-4	45.0	42
CCAGCGA	20	7.0292765E-4	45.0	41
>>END_MODULE