##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3554320_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1116935 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.310150545913594 34.0 31.0 34.0 30.0 34.0 2 32.50604108564957 34.0 31.0 34.0 31.0 34.0 3 32.587871272724016 34.0 31.0 34.0 31.0 34.0 4 36.11000013429609 37.0 35.0 37.0 35.0 37.0 5 35.940600840693506 37.0 35.0 37.0 35.0 37.0 6 35.92523468241214 37.0 35.0 37.0 35.0 37.0 7 35.97492423462422 37.0 35.0 37.0 35.0 37.0 8 36.084704123337524 37.0 35.0 37.0 35.0 37.0 9 37.70885951286333 39.0 38.0 39.0 35.0 39.0 10 37.322937324016166 39.0 37.0 39.0 34.0 39.0 11 37.284759632386844 39.0 37.0 39.0 34.0 39.0 12 37.226270105243366 39.0 37.0 39.0 34.0 39.0 13 37.209401621401426 39.0 37.0 39.0 34.0 39.0 14 38.48635327928662 40.0 38.0 41.0 34.0 41.0 15 38.50621656586999 40.0 38.0 41.0 34.0 41.0 16 38.4837676319571 40.0 38.0 41.0 34.0 41.0 17 38.46480502446427 40.0 38.0 41.0 34.0 41.0 18 38.39664349313075 40.0 38.0 41.0 34.0 41.0 19 38.39056525223043 40.0 38.0 41.0 34.0 41.0 20 38.30524694812142 40.0 38.0 41.0 34.0 41.0 21 38.27417172888306 40.0 38.0 41.0 34.0 41.0 22 38.22449381566519 40.0 37.0 41.0 34.0 41.0 23 38.16658444761781 40.0 37.0 41.0 34.0 41.0 24 38.10251088917439 40.0 37.0 41.0 33.0 41.0 25 38.03748382851285 40.0 37.0 41.0 33.0 41.0 26 37.927390582263065 40.0 37.0 41.0 33.0 41.0 27 37.85148643385694 40.0 37.0 41.0 33.0 41.0 28 37.79743046820092 40.0 37.0 41.0 33.0 41.0 29 37.7099965530671 40.0 37.0 41.0 33.0 41.0 30 37.69151562087319 40.0 37.0 41.0 33.0 41.0 31 37.65303710600885 40.0 37.0 41.0 33.0 41.0 32 37.58908620465829 40.0 36.0 41.0 33.0 41.0 33 37.50035857055245 40.0 36.0 41.0 33.0 41.0 34 37.38604036940377 40.0 36.0 41.0 32.0 41.0 35 37.3556536414384 40.0 36.0 41.0 32.0 41.0 36 37.21383070635265 39.0 36.0 41.0 31.0 41.0 37 37.14449900844723 39.0 36.0 41.0 31.0 41.0 38 37.085862650915224 39.0 35.0 41.0 31.0 41.0 39 37.03176997766209 39.0 35.0 41.0 31.0 41.0 40 36.931033587451374 39.0 35.0 41.0 31.0 41.0 41 36.83910881116627 39.0 35.0 41.0 31.0 41.0 42 36.75619440701563 39.0 35.0 41.0 31.0 41.0 43 36.66944450661856 39.0 35.0 41.0 30.0 41.0 44 36.580499312851686 39.0 35.0 41.0 30.0 41.0 45 36.47860618567777 39.0 35.0 41.0 30.0 41.0 46 36.38724455765107 39.0 35.0 40.0 30.0 41.0 47 36.23601104809143 39.0 35.0 40.0 30.0 41.0 48 36.131640605764886 38.0 35.0 40.0 29.0 41.0 49 35.97629226409773 38.0 35.0 40.0 29.0 41.0 50 35.84993397109053 38.0 35.0 40.0 28.0 41.0 51 34.81127729008402 37.0 33.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 3.0 10 6.0 11 13.0 12 14.0 13 20.0 14 27.0 15 55.0 16 83.0 17 156.0 18 303.0 19 487.0 20 807.0 21 1274.0 22 1795.0 23 2686.0 24 3660.0 25 4844.0 26 6216.0 27 8163.0 28 10360.0 29 13500.0 30 17203.0 31 22669.0 32 29048.0 33 39030.0 34 59395.0 35 75973.0 36 92614.0 37 135947.0 38 231607.0 39 358841.0 40 134.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.220961828575522 33.27418336787727 20.94660835232131 25.5582464512259 2 22.847435168563972 31.541405721908617 20.430374193663912 25.1807849158635 3 21.688280875789548 31.84957047634822 20.979197536114455 25.482951111747777 4 22.499160649455877 31.71402095914265 19.347500078339383 26.43931831306208 5 19.58708429765385 34.34147913710287 16.84556397641761 29.22587258882567 6 20.33976910026098 38.14975804321648 19.252418448701132 22.258054407821405 7 85.60659304256738 6.408072090139534 4.275629289081281 3.7097055782118025 8 86.9850080801479 5.7350696325211405 3.937740334039134 3.3421819532918207 9 77.05112652034362 12.021558998509313 6.516762389933166 4.410552091213902 10 31.845899716635255 40.714007529533944 14.330108735065156 13.109984018765639 11 25.834090614046474 29.224529627955075 27.93376516986217 17.00761458813628 12 27.709490704472508 25.84948989869599 28.774906328479275 17.66611306835223 13 23.701289690089396 26.829582742057507 27.93770452174925 21.531423046103846 14 20.068311942951023 29.584443141275006 27.94773196291637 22.3995129528576 15 20.60316849234736 31.48500136534355 28.215249768339252 19.696580373969837 16 22.0234839090905 29.8591234046744 28.21193713152511 19.905455554709988 17 20.929687045351788 30.15000872924566 28.27299708577491 20.647307139627642 18 21.43544610921853 30.6307887209193 27.01356838132926 20.920196788532905 19 23.017722606955644 29.587845308813854 27.28717427603218 20.107257808198327 20 23.985191618133552 29.041886949553913 28.403891005295744 18.56903042701679 21 23.64112504308666 29.334741950068715 27.02422253756933 19.999910469275296 22 22.775452465899985 29.52338318702521 26.909981332843895 20.791183014230906 23 22.75736725950928 29.3887289770667 27.98918468845546 19.864719074968555 24 21.36811900423928 28.615273046327673 29.547108829072418 20.46949912036063 25 20.52223271721273 32.202948246764585 26.03777301275365 21.237046023269034 26 21.56866782758173 31.31757891014249 26.74998992779347 20.36376333448231 27 21.879339442313118 30.592200978570823 27.040427598741196 20.488031980374867 28 20.998446641926343 30.88272818024326 28.658426855636183 19.46039832219422 29 20.518293365325647 30.169884550130494 27.65613039254746 21.655691691996402 30 21.04043655181367 30.331756100399755 27.515298562584217 21.112508785202362 31 21.697054886810783 30.751565668548302 26.434036000304406 21.117343444336512 32 22.138620421063 30.5725042191354 27.396670352348167 19.892205007453434 33 21.0908423498234 31.432625891390277 26.47674215598938 20.99978960279694 34 20.195356041309477 30.75899671869894 28.887625510884696 20.15802172910689 35 23.51193220733525 28.896578583355343 27.670097185601666 19.921392023707735 36 21.100064014468163 32.29516489321223 27.27267029862973 19.332100793689875 37 20.971139770890876 32.56483143602806 27.576089924659897 18.88793886842117 38 20.998088519027515 30.459695506005275 28.570418153249744 19.97179782171747 39 21.74611772394992 29.806926992170542 27.86581134980997 20.581143934069573 40 22.147752554983054 30.130043377636117 29.25255274478819 18.469651322592632 41 19.65539624060487 29.549705220088907 30.672868161531337 20.122030377774895 42 20.19168528159651 30.380729406814183 28.196448316150896 21.23113699543841 43 21.11286690810119 30.529350409826893 27.849337696463984 20.508444985607934 44 21.127102293329514 30.173197186944627 27.632673342674373 21.067027177051482 45 19.950579039962037 30.962947709580234 28.048006374587597 21.038466875870128 46 19.103528853514305 31.571219453235866 28.20396889702624 21.121282796223596 47 21.121551388397712 30.82954692976762 28.298423811591544 19.75047787024312 48 20.80255341626863 28.837935958672617 29.41612537882688 20.943385246231873 49 19.951474347209103 31.892366162757906 27.95999767220116 20.196161817831833 50 19.64733847538129 29.93003173864191 29.597872749980976 20.824757035995827 51 20.5148911977868 28.94555188976977 28.44677622243013 22.092780690013296 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5258.0 1 4143.0 2 3028.0 3 2133.5 4 1239.0 5 1221.5 6 1204.0 7 1444.5 8 1685.0 9 2077.5 10 2470.0 11 2503.0 12 2536.0 13 2770.5 14 3005.0 15 3575.5 16 4146.0 17 4177.0 18 4208.0 19 4741.5 20 5275.0 21 5587.5 22 5900.0 23 8572.5 24 11245.0 25 12986.0 26 16406.0 27 18085.0 28 22723.5 29 27362.0 30 32639.0 31 37916.0 32 41321.0 33 44726.0 34 48150.5 35 51575.0 36 55573.0 37 59571.0 38 62726.0 39 65881.0 40 66361.5 41 66842.0 42 68365.5 43 69889.0 44 70945.5 45 72002.0 46 72467.0 47 72932.0 48 73238.0 49 73544.0 50 70382.0 51 67220.0 52 62428.0 53 57636.0 54 52402.0 55 47168.0 56 44019.0 57 40870.0 58 40845.0 59 40820.0 60 39824.0 61 38828.0 62 35186.0 63 31544.0 64 26877.5 65 22211.0 66 18574.0 67 14937.0 68 12351.0 69 9765.0 70 8575.5 71 7386.0 72 6247.0 73 5108.0 74 4126.5 75 2436.5 76 1728.0 77 1371.0 78 1014.0 79 806.5 80 599.0 81 474.0 82 349.0 83 253.0 84 157.0 85 135.0 86 113.0 87 74.5 88 36.0 89 31.5 90 27.0 91 19.5 92 12.0 93 9.5 94 7.0 95 4.0 96 1.0 97 1.0 98 1.0 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1116935.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.685070659202406 #Duplication Level Percentage of deduplicated Percentage of total 1 73.62614433039431 15.965881421692348 2 8.31278186952136 3.6052652443021462 3 3.158185240124764 2.0545641026086683 4 1.7753039060125702 1.5399036257376242 5 1.2378836396632733 1.3421797096984363 6 0.9332637254364984 1.2142733897856564 7 0.7598214511885778 1.1533747300181414 8 0.6604841388515924 1.1458116176223385 9 0.5462899256357291 1.0661702074039092 >10 7.335450927075543 35.90911963188526 >50 1.1672923374600515 17.63927825991688 >100 0.4783780749773327 15.113219396521771 >500 0.005398363693320595 0.8345755445410842 >1k 0.0033220699651203664 1.416383118265722 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3992 0.35740665302815294 No Hit AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 3247 0.29070626312184683 No Hit AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA 2717 0.24325497902742774 No Hit AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 1405 0.12579066821256385 No Hit CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA 1246 0.11155528298423811 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 8.953072470645113E-5 0.0 0.0 0.0905155626782221 0.0 2 8.953072470645113E-5 0.0 0.0 0.1293718972008219 0.0 3 8.953072470645113E-5 0.0 0.0 0.17834520361525066 0.0 4 8.953072470645113E-5 0.0 0.0 0.37773012753651736 0.0 5 8.953072470645113E-5 0.0 0.0 0.42016769104737517 0.0 6 8.953072470645113E-5 0.0 0.0 0.7417620541929477 0.0 7 8.953072470645113E-5 0.0 0.0 0.9733780390085367 0.0 8 8.953072470645113E-5 0.0 0.0 1.2382994534149256 0.0 9 8.953072470645113E-5 0.0 0.0 1.529095247261479 0.0 10 8.953072470645113E-5 0.0 0.0 1.987313496309096 0.0 11 8.953072470645113E-5 0.0 0.0 2.3393483058548616 0.0 12 8.953072470645113E-5 0.0 0.0 2.5073079454041642 0.0 13 8.953072470645113E-5 0.0 0.0 2.5903029272070444 0.0 14 8.953072470645113E-5 0.0 0.0 2.6782220988687793 0.0 15 8.953072470645113E-5 0.0 0.0 2.761127549946953 0.0 16 8.953072470645113E-5 0.0 0.0 2.909748552959662 0.0 17 8.953072470645113E-5 0.0 0.0 3.0853183041090126 0.0 18 8.953072470645113E-5 0.0 0.0 3.2795999767220114 0.0 19 8.953072470645113E-5 0.0 0.0 3.393393527823911 0.0 20 8.953072470645113E-5 0.0 0.0 3.513275168205849 0.0 21 8.953072470645113E-5 0.0 0.0 3.6618066404938516 0.0 22 8.953072470645113E-5 0.0 0.0 3.8319150174361085 0.0 23 8.953072470645113E-5 0.0 0.0 4.00846960655723 0.0 24 8.953072470645113E-5 0.0 0.0 4.148853782896945 0.0 25 8.953072470645113E-5 0.0 0.0 4.2731224287895 0.0 26 8.953072470645113E-5 0.0 0.0 4.384050996700793 0.0 27 8.953072470645113E-5 0.0 0.0 4.504559352155676 0.0 28 8.953072470645113E-5 0.0 0.0 4.62632113775645 0.0 29 8.953072470645113E-5 0.0 0.0 4.764019392354972 0.0 30 1.7906144941290226E-4 0.0 0.0 4.934306830746642 0.0 31 1.7906144941290226E-4 0.0 0.0 5.079883789119331 0.0 32 1.7906144941290226E-4 0.0 0.0 5.234951004310904 0.0 33 1.7906144941290226E-4 0.0 0.0 5.375514242100032 0.0 34 1.7906144941290226E-4 0.0 0.0 5.525299144533926 0.0 35 1.7906144941290226E-4 0.0 0.0 5.678307153057251 0.0 36 1.7906144941290226E-4 0.0 0.0 5.8380299659335595 0.0 37 1.7906144941290226E-4 0.0 0.0 6.0080488121511095 0.0 38 1.7906144941290226E-4 0.0 0.0 6.175381736627467 0.0 39 1.7906144941290226E-4 0.0 0.0 6.349250404007395 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCGTGC 30 2.1654469E-6 45.000004 36 TATCTCG 30 2.1654469E-6 45.000004 1 CGTTTAG 30 2.1654469E-6 45.000004 1 AATCGCG 30 2.1654469E-6 45.000004 1 ATTACCG 35 1.2118835E-7 45.0 1 GACCGAA 20 7.0332014E-4 45.0 25 TATGCGA 25 3.8907354E-5 45.0 24 ATCGTAC 35 1.2118835E-7 45.0 28 CGTTACG 20 7.0332014E-4 45.0 1 AATTGCG 40 6.8139343E-9 45.0 1 TATTCGT 20 7.0332014E-4 45.0 33 GCGATCG 20 7.0332014E-4 45.0 9 TTACGGT 20 7.0332014E-4 45.0 31 TAGTCCG 50 2.1827873E-11 45.0 1 AATAGCG 110 0.0 42.954548 1 TACGTAG 90 0.0 42.5 1 CATACCG 80 0.0 42.1875 1 CCATGCG 70 0.0 41.785713 1 ACGATAG 60 3.6379788E-12 41.250004 1 CGTTCGA 55 6.184564E-11 40.909092 42 >>END_MODULE