FastQCFastQC Report
Sat 18 Jun 2016
SRR3554318_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3554318_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1023642
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50520.49353191838552934No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA42270.4129373355137831No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG31390.3066501765265591No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG19980.1951854261548471No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG19110.18668636105200842No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT16700.16314297381311044No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA12750.1245552644381532No Hit
AAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG12560.12269914677201599No Hit
AGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAAGGAAGGTGAA10870.10618946858374315No Hit
CTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGCT10330.10091418679577431No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAT302.1652686E-645.00000424
ACGTTGC207.0329226E-445.00000428
TAACGCG302.1652686E-645.0000041
TAACCCG302.1652686E-645.0000041
ATATACG207.0329226E-445.0000041
TCATGCG302.1652686E-645.0000041
TCGTATA406.8139343E-945.00000423
CGTAATG207.0329226E-445.0000041
CGTAACG406.8139343E-945.0000041
ATACGCA207.0329226E-445.00000432
TCGACCA207.0329226E-445.00000426
CGTTTAG406.8139343E-945.0000041
AAATGCG207.0329226E-445.0000041
CGCATAG502.1827873E-1145.01
TTAGTCG351.2117562E-745.028
ATACGCG253.890506E-545.01
GTTGCGT253.890506E-545.042
TATACGG900.042.52
TCAAACG700.041.7857131
TACGGGT1300.041.538464