##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3554318_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1023642
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31830464166183	34.0	31.0	34.0	30.0	34.0
2	32.49270936518822	34.0	31.0	34.0	30.0	34.0
3	32.59786233859103	34.0	31.0	34.0	31.0	34.0
4	36.10967896979608	37.0	35.0	37.0	35.0	37.0
5	35.94792124590433	37.0	35.0	37.0	35.0	37.0
6	35.91145537209298	37.0	35.0	37.0	35.0	37.0
7	35.92153897554028	37.0	35.0	37.0	35.0	37.0
8	36.04371450174963	37.0	35.0	37.0	35.0	37.0
9	37.67649920577702	39.0	38.0	39.0	35.0	39.0
10	37.336797435040765	39.0	37.0	39.0	34.0	39.0
11	37.28058149235768	39.0	37.0	39.0	34.0	39.0
12	37.191975319496464	39.0	37.0	39.0	34.0	39.0
13	37.16238196556999	39.0	37.0	39.0	33.0	39.0
14	38.40832341775738	40.0	38.0	41.0	34.0	41.0
15	38.432595575406246	40.0	38.0	41.0	34.0	41.0
16	38.425517905674056	40.0	38.0	41.0	34.0	41.0
17	38.3878592320362	40.0	38.0	41.0	34.0	41.0
18	38.29901664839856	40.0	38.0	41.0	34.0	41.0
19	38.29559650737269	40.0	37.0	41.0	34.0	41.0
20	38.200068969424855	40.0	37.0	41.0	34.0	41.0
21	38.177635345169506	40.0	37.0	41.0	34.0	41.0
22	38.1045492467093	40.0	37.0	41.0	33.0	41.0
23	38.04879342582661	40.0	37.0	41.0	33.0	41.0
24	37.972384876744016	40.0	37.0	41.0	33.0	41.0
25	37.91239026925429	40.0	37.0	41.0	33.0	41.0
26	37.806803550460025	40.0	37.0	41.0	33.0	41.0
27	37.72911037257166	40.0	37.0	41.0	33.0	41.0
28	37.65203948255347	40.0	36.0	41.0	33.0	41.0
29	37.55500555858396	40.0	36.0	41.0	33.0	41.0
30	37.530511643719194	40.0	36.0	41.0	33.0	41.0
31	37.473897124189904	40.0	36.0	41.0	32.0	41.0
32	37.4121968422554	40.0	36.0	41.0	32.0	41.0
33	37.331106969038004	40.0	36.0	41.0	32.0	41.0
34	37.18422065526815	39.0	36.0	41.0	31.0	41.0
35	37.185386101781674	40.0	35.0	41.0	31.0	41.0
36	37.05557411673222	39.0	35.0	41.0	31.0	41.0
37	36.94833545321509	39.0	35.0	41.0	31.0	41.0
38	36.87711133384523	39.0	35.0	41.0	31.0	41.0
39	36.79788441662222	39.0	35.0	41.0	31.0	41.0
40	36.7060310147493	39.0	35.0	41.0	31.0	41.0
41	36.59140695672901	39.0	35.0	41.0	30.0	41.0
42	36.51209211814287	39.0	35.0	41.0	30.0	41.0
43	36.441180608064144	39.0	35.0	41.0	30.0	41.0
44	36.342657882345584	39.0	35.0	40.0	30.0	41.0
45	36.21446560418584	39.0	35.0	40.0	30.0	41.0
46	36.11432903300177	38.0	35.0	40.0	29.0	41.0
47	35.957032829837	38.0	35.0	40.0	29.0	41.0
48	35.850756416794155	38.0	35.0	40.0	29.0	41.0
49	35.705623645766785	38.0	34.0	40.0	28.0	41.0
50	35.60228771386872	38.0	34.0	40.0	28.0	41.0
51	34.49904068023782	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	4.0
11	7.0
12	14.0
13	14.0
14	35.0
15	46.0
16	67.0
17	124.0
18	309.0
19	511.0
20	955.0
21	1323.0
22	2055.0
23	3082.0
24	4058.0
25	5313.0
26	6694.0
27	8145.0
28	10073.0
29	12745.0
30	16386.0
31	21245.0
32	27710.0
33	37281.0
34	58875.0
35	73947.0
36	85663.0
37	123260.0
38	206853.0
39	316698.0
40	147.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.46994945498524	34.158035719519134	19.689696202383256	25.682318623112376
2	22.08125496999928	33.1565137030329	20.135066751852698	24.627164575115128
3	22.582406739856317	31.006152541611225	20.754814671535556	25.656626046996898
4	23.1917994767702	31.426807419000003	19.187372147684446	26.194020956545355
5	19.394964255081366	35.1266360700323	16.835084922267747	28.643314752618593
6	21.166188960593647	38.672406954775205	19.30157222935362	20.859831855277527
7	83.17771252058826	7.460518423433192	5.192831087430958	4.168937968547598
8	84.53443684413105	6.419236412730232	4.873774229662323	4.172552513476392
9	73.94987700778202	12.68490351118848	7.850986966146367	5.514232514883133
10	34.33651608667874	35.33911269760326	15.82897145681791	14.495399758900085
11	27.466438461884135	29.94347633254595	25.04996864138048	17.540116564189432
12	28.797958661328867	25.8704703402166	27.498773985436316	17.832797013018222
13	23.904744041373842	27.957918881796566	26.442936104614702	21.694400972214893
14	20.219080498846274	29.145736497720883	28.34594516442272	22.289237839010124
15	21.132583461796216	30.696278581769796	28.292801584929105	19.878336371504883
16	22.905078142553744	29.14856951942183	28.254800017975036	19.691552320049393
17	22.427958211953005	29.758255327546152	27.469857626005968	20.343928834494875
18	21.263390912057144	30.667557603146413	26.60637214963825	21.462679335158192
19	22.845975448447795	30.216325629468116	26.707970169258395	20.229728752825697
20	23.982310221737677	29.070319506233627	27.856223171772942	19.091147100255753
21	23.76338602753697	30.179398656952333	26.88693898843541	19.170276327075285
22	22.492824639864327	30.148430799048885	26.113328683270126	21.245415877816658
23	22.6929922765967	29.215585136209732	27.629386054890283	20.462036532303284
24	21.79101678125751	28.934627535798647	28.740419013678608	20.53393666926523
25	21.312040732990635	31.249010884664756	26.194802479773198	21.244145902571407
26	22.27409582647058	31.078150368976655	25.874084885145393	20.77366891940737
27	21.740706223464844	30.251103413107316	26.30978408467023	21.698406278757613
28	20.89656344698635	30.687388755053036	28.136985391377063	20.279062406583552
29	20.775231965863064	30.198057524017187	26.77576730927414	22.250943200845608
30	21.080807547951334	31.122208740946544	26.636753865120816	21.16022984598131
31	22.11173437588532	30.09128191301256	26.148790299733697	21.64819341136843
32	22.17669849420012	30.026903937118643	26.717641519203	21.078756049478237
33	21.532234902436596	30.956037364625523	26.40942829622075	21.102299436717136
34	19.86739504631502	30.615781689301535	28.16560867959697	21.35121458478648
35	23.29877046858179	27.898132354866252	28.00910865322056	20.7939885233314
36	21.986886040236723	32.11884623725873	25.965913864417445	19.928353858087107
37	20.447773733395074	33.38667229363391	26.666256366972046	19.499297605998972
38	21.176153381748698	31.44947159260757	27.47552366940786	19.898851356235873
39	21.5828385314397	30.572504840559496	27.670904476369667	20.173752151631135
40	22.197701930948515	30.27396296752185	28.100742251685645	19.42759284984399
41	19.531926200761596	30.782246136832995	29.391232481668396	20.294595180737016
42	20.377925094906228	30.366475779618263	27.615220946385556	21.640378179089957
43	21.91527897448522	29.053125995220984	27.501900078347703	21.52969495194609
44	21.358639055451025	29.725822113590496	26.62815710961449	22.287381721343984
45	20.249169143118394	31.25809609218848	27.73450092903574	20.75823383565739
46	19.49382694340404	31.067892876611158	28.707106586091623	20.731173593893175
47	20.60261302291231	30.0485912066914	28.96061318312457	20.38818258727172
48	20.8287663069706	28.882656241146808	29.116136305466167	21.172441146416425
49	19.459537611782242	31.194792710732855	28.217482283845328	21.12818739363957
50	19.628053557786803	29.508656346652444	28.608146207365465	22.255143888195285
51	20.434780909732115	28.51875948818044	28.28586556628196	22.760594035805486
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6540.0
1	5034.5
2	3529.0
3	2543.0
4	1557.0
5	1437.5
6	1318.0
7	1482.5
8	1647.0
9	1837.0
10	2027.0
11	2243.0
12	2459.0
13	2832.0
14	3205.0
15	3115.0
16	3025.0
17	3200.0
18	3375.0
19	3840.0
20	4305.0
21	5120.0
22	5935.0
23	9023.0
24	12111.0
25	12859.5
26	14827.5
27	16047.0
28	20358.0
29	24669.0
30	28024.0
31	31379.0
32	36310.5
33	41242.0
34	41647.5
35	42053.0
36	46247.0
37	50441.0
38	52582.5
39	54724.0
40	56633.0
41	58542.0
42	57790.0
43	57038.0
44	62168.5
45	67299.0
46	69309.0
47	71319.0
48	69929.5
49	68540.0
50	64769.5
51	60999.0
52	56614.0
53	52229.0
54	48039.0
55	43849.0
56	41826.0
57	39803.0
58	38449.0
59	37095.0
60	37684.5
61	38274.0
62	33804.5
63	29335.0
64	25399.5
65	21464.0
66	18916.5
67	16369.0
68	14149.5
69	11930.0
70	10463.5
71	8997.0
72	7348.0
73	5699.0
74	4966.0
75	3389.0
76	2545.0
77	1922.5
78	1300.0
79	1068.5
80	837.0
81	605.0
82	373.0
83	265.5
84	158.0
85	130.5
86	103.0
87	88.0
88	73.0
89	50.0
90	27.0
91	19.0
92	11.0
93	7.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1023642.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.252947235846165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.06837995749736	16.704926982746404
2	8.06236536507179	3.588227821301123
3	3.077225651712529	2.0543202018105373
4	1.7307908984147362	1.5406079415482363
5	1.159192080763267	1.2897720104717854
6	0.8535437264973916	1.1396318105540382
7	0.7080332075789562	1.1029077926636999
8	0.5937775919934712	1.0570641139566805
9	0.5167364122550904	1.0349017305077681
>10	6.694604902592295	33.92445137633568
>50	0.9972498750855119	15.397991787272488
>100	0.5266326185753744	17.902587304750377
>500	0.007057053515247898	1.039704535741342
>1k	0.003969592602326943	1.7270500340701076
>5k	4.410658447029936E-4	0.4958545562697309
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5052	0.49353191838552934	No Hit
AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	4227	0.4129373355137831	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3139	0.3066501765265591	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1998	0.1951854261548471	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1911	0.18668636105200842	No Hit
ACAGGCAGAATTTGAAACCTAAAGAACTCCCAGATTTCAGGCTTATCCTGT	1670	0.16314297381311044	No Hit
CACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAA	1275	0.1245552644381532	No Hit
AAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1256	0.12269914677201599	No Hit
AGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCAAGGAAGGTGAA	1087	0.10618946858374315	No Hit
CTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGCT	1033	0.10091418679577431	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13705963608370894	0.0
2	0.0	0.0	0.0	0.18561176661371848	0.0
3	0.0	0.0	0.0	0.2604426156800913	0.0
4	0.0	0.0	0.0	0.6061689535990121	0.0
5	0.0	0.0	0.0	0.6663462421432493	0.0
6	0.0	0.0	0.0	1.038742060212457	0.0
7	0.0	0.0	0.0	1.3120798091520278	0.0
8	0.0	0.0	0.0	1.56832173748244	0.0
9	0.0	0.0	0.0	1.969340843771553	0.0
10	0.0	0.0	0.0	2.5157232704402515	0.0
11	0.0	0.0	0.0	3.0096459504397046	0.0
12	0.0	0.0	0.0	3.2160657729948556	0.0
13	0.0	0.0	0.0	3.31268158203747	0.0
14	0.0	0.0	0.0	3.4433913418949205	0.0
15	0.0	0.0	0.0	3.5465524079707555	0.0
16	0.0	0.0	0.0	3.733141078619283	0.0
17	0.0	0.0	0.0	3.944054659734556	0.0
18	0.0	0.0	0.0	4.2015665633102195	0.0
19	0.0	0.0	0.0	4.338333128183486	0.0
20	0.0	0.0	0.0	4.487506374298827	0.0
21	0.0	0.0	0.0	4.665107527827112	0.0
22	0.0	0.0	0.0	4.864005189314233	0.0
23	0.0	0.0	0.0	5.050789240769722	0.0
24	0.0	0.0	0.0	5.2009393909198725	0.0
25	0.0	0.0	0.0	5.343860451212436	0.0
26	0.0	0.0	0.0	5.485413845856266	0.0
27	0.0	0.0	0.0	5.622668862746937	0.0
28	0.0	0.0	0.0	5.767641421512599	0.0
29	0.0	0.0	0.0	5.928342135238687	0.0
30	0.0	0.0	0.0	6.109264762485322	0.0
31	0.0	0.0	0.0	6.265862479265212	0.0
32	0.0	0.0	0.0	6.436722994953314	0.0
33	0.0	0.0	0.0	6.606020464185721	0.0
34	0.0	0.0	0.0	6.777369431891228	0.0
35	0.0	0.0	0.0	6.95799898792742	0.0
36	0.0	0.0	0.0	7.1438061353481	0.0
37	0.0	0.0	0.0	7.317206601526705	0.0
38	0.0	0.0	0.0	7.501059940877767	0.0
39	0.0	0.0	0.0	7.702888314469316	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTAT	30	2.1652686E-6	45.000004	24
ACGTTGC	20	7.0329226E-4	45.000004	28
TAACGCG	30	2.1652686E-6	45.000004	1
TAACCCG	30	2.1652686E-6	45.000004	1
ATATACG	20	7.0329226E-4	45.000004	1
TCATGCG	30	2.1652686E-6	45.000004	1
TCGTATA	40	6.8139343E-9	45.000004	23
CGTAATG	20	7.0329226E-4	45.000004	1
CGTAACG	40	6.8139343E-9	45.000004	1
ATACGCA	20	7.0329226E-4	45.000004	32
TCGACCA	20	7.0329226E-4	45.000004	26
CGTTTAG	40	6.8139343E-9	45.000004	1
AAATGCG	20	7.0329226E-4	45.000004	1
CGCATAG	50	2.1827873E-11	45.0	1
TTAGTCG	35	1.2117562E-7	45.0	28
ATACGCG	25	3.890506E-5	45.0	1
GTTGCGT	25	3.890506E-5	45.0	42
TATACGG	90	0.0	42.5	2
TCAAACG	70	0.0	41.785713	1
TACGGGT	130	0.0	41.53846	4
>>END_MODULE